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Articles, Cellular/Molecular

Cannabinoid CB1 Receptor Calibrates Excitatory Synaptic Balance in the Mouse Hippocampus

Krisztina Monory, Martin Polack, Anita Remus, Beat Lutz and Martin Korte
Journal of Neuroscience 4 March 2015, 35 (9) 3842-3850; https://doi.org/10.1523/JNEUROSCI.3167-14.2015
Krisztina Monory
1Institute of Physiological Chemistry, Medical Center of the Johannes Gutenberg University Mainz, 55128 Mainz, Germany,
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Martin Polack
2Zoological Institute, Division Cellular Neurobiology, 38106 Braunschweig, Germany, and
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Anita Remus
2Zoological Institute, Division Cellular Neurobiology, 38106 Braunschweig, Germany, and
3AG NIND, HZI, D-38124 Braunschweig, Germany
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Beat Lutz
1Institute of Physiological Chemistry, Medical Center of the Johannes Gutenberg University Mainz, 55128 Mainz, Germany,
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Martin Korte
2Zoological Institute, Division Cellular Neurobiology, 38106 Braunschweig, Germany, and
3AG NIND, HZI, D-38124 Braunschweig, Germany
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Abstract

The endocannabinoid system negatively regulates the release of various neurotransmitters in an activity-dependent manner, thereby influencing the excitability of neuronal circuits. In the hippocampus, cannabinoid type 1 (CB1) receptor is present on both GABAergic and glutamatergic axon terminals. CB1 receptor-deficient mice were previously shown to have increased hippocampal long-term potentiation (LTP). In this study, we have investigated the consequences of cell-type-specific deletion of the CB1 receptor on the induction of hippocampal LTP and on CA1 pyramidal cell morphology. Deletion of CB1 receptor in GABAergic neurons in GABA-CB1-KO mice leads to a significantly decreased hippocampal LTP compared with WT controls. Concomitantly, CA1 pyramidal neurons have a significantly reduced dendritic branching both on the apical and on the basal dendrites. Moreover, the average spine density on the apical dendrites of CA1 pyramidal neurons is significantly diminished. In contrast, in mice lacking CB1 receptor in glutamatergic cells (Glu-CB1-KO), hippocampal LTP is significantly enhanced and CA1 pyramidal neurons show an increased branching and an increased spine density in the apical dendritic region. Together, these results indicate that the CB1 receptor signaling system both on inhibitory and excitatory neurons controls functional and structural synaptic plasticity of pyramidal neurons in the hippocampal CA1 region to maintain an appropriate homeostatic state upon neuronal activation. Consequently, if the CB1 receptor is lost in either neuronal population, an allostatic shift will occur leading to a long-term dysregulation of neuronal functions.

  • cannabinoids
  • CB1 receptor
  • dendritic morphology
  • LTP
  • spines
  • synaptic plasticity

Introduction

To perform its tremendous task, the brain must process and store new information, and at the same time, maintain and retrieve already stored information. To accomplish this, tightly regulated processes must be in place. Synaptic plasticity needs on the one hand cellular mechanisms to regulate and maintain synaptic strength (Bliss and Collingridge, 1993; Malenka and Bear, 2004). On the other hand, homeostatic mechanisms need to operate to restrict the number of synapses which can be potentiated (Turrigiano, 2011). Even the amount of potentiation at single synapses may be restricted and tightly regulated (Roth-Alpermann et al., 2006). Endocannabinoids, acting through cannabinoid type 1 (CB1) receptor, are important negative regulators of transmitter release and as such effectively influence not only synaptic plasticity (Kano et al., 2009; Castillo et al., 2012), but they are also involved in homeostatic processes; e.g., after long-term silencing of neuronal activity (Kim and Alger, 2010) or after excessive activation of excitatory transmission (Bojnik et al., 2012). Expression of CB1 receptor is especially prominent in the hippocampus, where cholecystokinin-positive inhibitory interneurons contain high levels and excitatory pyramidal neurons in the CA3 and CA1 region provide low levels of this receptor (Marsicano and Lutz, 1999). In fact, genetic disruption of the CB1 receptor leads to an enhanced performance in certain learning paradigms (Marsicano and Lafenetre, 2009; Ruehle et al., 2012). However, CB1-KO mice showed a deficit to unlearn the water maze task (Varvel and Lichtman, 2002) and in extinction of fear memory (Marsicano et al., 2002). Furthermore, administration of cannabinoids impairs certain memory processes via CB1 receptor-mediated signaling, whereas CB1 receptor blockade enhances performance in several different paradigms (Riedel and Davies, 2005).

Long-term potentiation (LTP) of synaptic transmission is a commonly used model for the synaptic changes that may underlie learning and memory (Citri and Malenka, 2008). Several studies have shown that exogenously applied (endo)cannabinoids act at the CB1 receptor to prevent LTP (Stella et al., 1997; Paton et al., 1998; Hoffman et al., 2007); or to modulate LTP (Navakkode and Korte, 2014). In CB1-KO mice, LTP was increased in vitro and in vivo in the hippocampus (Bohme et al., 2000; Jacob et al., 2012) and in vitro in the amygdala (Marsicano et al., 2002), suggesting that endocannabinoids restrict potentiation. Interestingly, however, LTP stimulation paradigms increase the formation of endocannabinoids in hippocampal slices (Stella et al., 1997). Moreover, the endocannabinoid-elicited decrease of inhibitory transmission facilitates hippocampal LTP by disinhibiting pyramidal neurons (Carlson et al., 2002; Chevaleyre and Castillo, 2004). These seemingly contradictory findings might be explained by the fact that the CB1 receptor signaling in the hippocampus regulates the release of both glutamate and GABA, two neurotransmitters of opposing effects. To understand how the CB1 receptor-mediated regulation of glutamatergic and GABAergic transmission contributes to the fine tuning of synaptic strength, in the present study we measured hippocampal LTP in mice specifically lacking CB1 receptor in cortical glutamatergic (Glu-CB1-KO) or forebrain GABAergic (GABA-CB1-KO) neurons. Furthermore, we have identified morphological substrates of the modified hippocampal neurotransmission elicited by neuronal type-specific deletion of CB1 receptor.

Materials and Methods

Animals.

Mutant mice, lacking CB1 receptor specifically in cortical glutamatergic (Glu-CB1-KO; Monory et al., 2006) or GABAergic neurons (GABA-CB1-KO; Monory et al., 2006) and their wild-type (WT) littermates in a predominant C57BL/6N background (>7 backcrosses into C57BL/6N) were used in this study. Glu-CB1-KO mice were obtained by crossing CB1f/f (Marsicano et al., 2003) with NEX-Cre mice (Kleppisch et al., 2003). GABA-CB1-KO mice were generated by crossing CB1f/f (Marsicano et al., 2003) with Dlx5/6-Cre (Monory et al., 2006). Genotyping was performed by PCR as described for Glu-CB1-KO (Marsicano et al., 2003; Monory et al., 2006), GABA-CB1-KO (Monory et al., 2007), and for CB1f/f (Marsicano et al., 2003). X-gal histochemistry for Dlx5/6-Cre (Monory et al., 2006) and NEX-Cre (Monory et al., 2006, Goebbels et al., 2006) showed that Cre recombinase expression in these transgenic mice recapitulates GABAergic and cortical glutamatergic neuronal expression with high accuracy. The detailed anatomical characterization of CB1 receptor expression in GABA-CB1-KO and Glu-CB1-KO was described by Monory et al. (2007) and Bellocchio et al. (2010). The breeding of GABA-CB1-KO is detailed by Massa et al. (2010). In this study, 8-week-old male adult mice were used. Animals were housed in a temperature- and humidity-controlled room with a 12 h light/dark cycle and had access to food and water ad libitum. The experimental protocols were performed in accordance with the European Communities Council Directive of 24 November, 1986 (86/609/EEC).

Slice preparation.

Mice were anesthetized and decapitated. The brains were transferred into ice-cold carbogenated (95% O2, 5% CO2) ACSF (124 mm NaCl, 3 mm KCl, 1.25 mm KH2PO4, 2 mm MgSO4, 2.5 mm CaCl2, 26 mm NaHCO3, 10 mm d(+)-glucose). Transverse hippocampal slices (400 μm) were prepared as described previously (Rösch et al., 2005) and maintained at room temperature in carbogenated ACSF until transfer into a submerged recording chamber (Warner Instruments), perfused with carbogenated ACSF (32°C).

Electrophysiological recordings.

Field EPSPs were recorded in stratum radiatum of CA1 with glass micropipettes (3 m NaCl, 10–12 MΩ). Synaptic responses were evoked by delivering square pulses (0.2 ms, 20–120 μA) to the Schaffer collaterals with monopolar tungsten electrodes (WPI). Baseline stimulation was delivered at an intensity eliciting a fEPSP of 50% of the maximal amplitude for 20 min, before a theta-burst stimulation (TBS) was applied. A TBS consisting of four pulses at 100 Hz was repeated 10 times with 200 μs intervals. In total, three such stimulus trains were given at 0.1 Hz. Basal synaptic transmission was assessed by determining the strength of postsynaptic responses to afferent fiber stimulation. Presynapse function was assessed with the paired pulse paradigm, delivering two closely spaced stimuli at interstimulus intervals of 160, 80, 40, 20, and 10 ms. For analysis of fEPSP sizes, the initial slope of the fEPSP was calculated. The ratio of paired pulse facilitation (PPF) was calculated as EPSP2 slope/EPSP1 slope. All values are presented as mean ± SEM. Differences were detected with two-tailed Student's t test, accepting p < 0.05 as significant. In LTP experiments, t test was applied to the mean values 55–60 min (or 175–180 min) after TBS. The analysis of fEPSP responses during TBS application was calculated as follows: each TBS consisted of four single pulses at 100 Hz (defined as a “burst”) which were repeated 10 times. We always compared the fourth fEPSP response of the 10th burst between genotypes; this is the 40th fEPSP and therefore the last response in one TBS. This last fEPSP response was calculated in percentage (fourth EPSP response divided by the first EPSP response in that last burst). Significance was tested by ANOVA, followed by a Fisher's post hoc test. Differences under these circumstances would mainly indicate that CA3 axons could not follow faithfully the stimulus, either due to changes in presynaptic properties, or due to defects in myelination.

For all experiments, only littermates were used. All experiments were performed and analyzed without the experimenter knowing the genotype of the preparation.

DiOlistics and morphological analysis.

Hippocampal neurons were labeled using Diolistic on acute slices prepared as described above. Slices were immediately fixed after preparation in 4% PFA overnight at 4°C. Tungsten particles (50 mg; 0.7 and 1.7 μm in diameter; Bio-Rad) were spread on a glass slide, and 100 μl of dye solution prepared by dissolving 3 mg of lipophilic dye DiI (Invitrogen) in 100 μl of methanol using a hand-held gene gun (Bio-Rad; Helios Gene Gun System). To prevent clusters of large particles from landing on the tissue, a membrane filter (3 μm; Millipore) was inserted between the gene gun and the preparation. After shooting, slices were kept in PBS for 3 d at room temperature to allow dye diffusion. The slices were postfixed with 4% PFA, washed, and mounted using an antifading water-based mounting medium (Biomeda). The neurons were imaged using an Axioplan 2 imaging microscope (Zeiss) equipped with an ApoTome (Zeiss). Each neuron was first imaged with a 20× objective with a z-sectioning of 0.975 μm. The morphological reconstruction of the neurons and their processes was achieved using the Neurolucida software (MicroBrightField), and the Sholl analysis (Sholl, 1953), as well as the number, length, and volume of dendrites was performed with the Neuroexplorer software (MicroBrightField). The spine density of the pyramidal cells was measured for mid-apical dendrites using a LSM510 Meta confocal microscope (Zeiss) with a 40× water-immersion objective and a zoom 4, and by z-sectioning at 0.3 μm. The statistical analysis was performed using GraphPad Prism 4. All data shown are presented as ±SEM. The data obtained were compared between two different experimental conditions using a two-tailed Student's t test. All experiments were performed and analyzed without the experimenter knowing the genotype of the preparation.

Results

Increased hippocampal LTP in Glu-CB1-KO mice

Presynaptically located and activated CB1 receptor on glutamatergic terminals inhibits the release of glutamate (Kano et al., 2009; Castillo et al., 2012). Thus, it can be assumed that glutamatergic synapses where the CB1 receptor is genetically deleted (Glu-CB1-KO mice) will show increased glutamatergic synaptic transmission following neuronal activation. This may affect the induction of NMDA receptor-dependent LTP. Indeed, we observed that LTP was significantly increased (Fig. 1A; p < 0.001, t test) in Glu-CB1-KO mice (n = 9 slices from 5 mice) compared with littermate controls (n = 9 slices from 5 mice) for the whole range of 60 min after tetanization. Basal transmission was analyzed by modulation of the stimulus strength and the corresponding fEPSP size (Fig. 1B). Basal transmission is compromised at higher currents and comparable with controls at low current values: the slope values of Glu-CB1-KO mice stayed significantly lower at 125 μA (p = 0.045), 150 μA (p = 0.03), 175 μA (p = 0.015), and 200 μA (p = 0.009). In addition, we evaluated fEPSP responses during TBS as an indicator for the ability to follow the TBS was analyzed in GABA-CB1-KO compared with the corresponding controls. No significant differences were observed here (p > 0.1 for all conditions tested, ANOVA, followed by Fisher's post hoc test).

Figure 1.
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Figure 1.

Baseline synaptic transmission and LTP experiments in Glu-CB1-KO mice. Summary of a set of extracellular fEPSP recordings in Glu-CB1-KO mice and their WT littermates. A, LTP with 20 min baseline recording, followed by TBS inducing a long lasting potentiation in both genotypes. Glu-CB1-KO mice showed an increased potentiation, significant for 60 min after tetanization. Insets, Original traces of representative individual experiments 5 min before (gray), and 55 min after (black) TBS application. Vertical scale bar, 1 mV; horizontal scale bar, 4 ms. B, EPSP slopes plotted against stimulus intensities showing significantly decreased slopes from 125 to 200 μA for Glu-CB1-KO mice (p < 0.05). C, Glu-CB1-KO mice showed comparable PPF ratios to control littermates. Two-tailed Student's t test. Error bars = SEM.

The ability of the presynapse to react adequately to two temporary fast following stimuli was probed with PPF. The relation between the value of the second slope and the first slope is shown with different interstimulus intervals (ISI) in Figure 1C. PPF was not altered between genotypes at any ISI values tested (Fig. 1C).

These results demonstrate that CB1 receptor located on glutamatergic neurons has a strong effect on LTP by limiting the amount of possible potentiation, but it only mildly influences basal synaptic transmission in the mouse hippocampus and does not influence the glutamate release for processes of short-term plasticity, such as PPF.

Decreased hippocampal LTP in GABA-CB1-KO mice

In the hippocampus, the CB1 receptor is expressed in excitatory (glutamatergic), as well as in inhibitory (GABAergic) cells (Marsicano and Lutz, 1999), but the amount of CB1 receptor mRNA and protein on GABAergic neurons greatly outnumbers that on glutamatergic cells (Steindel et al., 2013). We therefore also investigated the role of CB1 receptor on GABAergic neurons for basal synaptic transmission and LTP. We performed electrophysiological recordings using 8-week-old GABA-CB1-KO mice, in which CB1 receptor is specifically deleted in forebrain GABAergic neurons (Monory et al., 2006) and their WT littermates. The summary data of all experiments are shown as mean with a stable baseline (3 ± 1%; Fig. 2). Unlike mice with CB1 receptor deficiency in the entire animal (Bohme et al., 2000; Jacob et al., 2012), GABA-CB1-KO mice (n = 10) showed a significantly (p < 0.05) decreased potentiation starting from 4 min after tetanization until the end of the experiment compared with littermate controls (n = 10; Fig. 2A).

Figure 2.
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Figure 2.

Baseline synaptic transmission and LTP induction in GABA-CB1-KO mice. Summary of a set of extracellular fEPSP recordings in GABA-CB1-KO mice and their WT littermates. A, LTP with 20 min of baseline recording, followed by a TBS inducing a long lasting potentiation in both genotypes. GABA-CB1-KO mice showed a decreased potentiation, significant from 4 min after tetanization. Insets, Original traces of representative individual experiments 5 min before (gray), and 55 min after (black) TBS application. Vertical scale bar, 1 mV; horizontal scale bar, 4 ms. B, EPSP slopes plotted against stimulus intensities showing significantly increased slopes at 25 and 50 μA for GABA-CB1-KO mice, (p < 0.05). C, GABA-CB1-KO mice showed comparable PPF ratios to control littermates (p = 0.11, t test). Two-tailed Student's t test.

Basal transmission was analyzed in the same way as for Glu-CB1-KO mice by modulation of the stimulus strength and the corresponding fEPSP size (Fig. 2B). The slope was significantly increased at 25 μA (p = 0.0008) and 50 μA (p = 0.023) stimulus strength in GABA-CB1-KO compared with littermate controls. With the application of higher currents, the responses of GABA-CB1-KO were indistinguishable from controls. In PPF experiments, both genotypes showed comparable values with a maximum at 20 ms ISI (Fig. 2C). There is a tendency for higher values in controls between 10 and 40 ms, but it does not reach statistical significance (p = 0.11, t test). In addition, we evaluated fEPSP responses during TBS as an indicator for the ability to follow the TBS. This was evaluated in Glu-CB1-KO mice compared with the corresponding controls. No significant differences were observed here (p > 0.1 for all conditions tested, ANOVA, followed by Fisher's post hoc test).

Together, these results showed a strong effect of CB1 receptor in GABAergic neurons on LTP, but no statistical significant effect on basal synaptic transmission.

Dendritic morphology of Glu-CB1-KO and GABA-CB1-KO mice

Because our electrophysiological experiments revealed an opposite phenotype in these two mutant lines, we next wondered whether the dendritic morphology of CA1 pyramidal neurons of the hippocampus would also be altered in the corresponding conditional CB1 receptor mutant mice. This has so far not been explored in the context of CB1 receptor signaling. Pyramidal neurons in the CA1 region were labeled with DiI in acute hippocampal slices (Fig. 3A–C). In view of their different morphology and connectivity, we analyzed the complexity of apical and basal dendrites separately. The apical and basal dendritic complexity was analyzed up to a distance of 700 and 300 μm from the cell body, respectively. The total dendritic complexity in animals lacking CB1 receptor on glutamatergic neurons (Glu-CB1-KO) was significantly increased compared with WT littermate controls in the apical dendrites (Glu-CB1-WT: 129.9 ± 14.4 intersections/neuron, n = 14; Glu-CB1-KO: 176.1 ± 13.1, n = 8; p = 0.044, t test). The total dendritic complexity in basal dendrites was comparable between Glu-CB1-KO and controls (Glu-CB1-WT: 97.4 ± 8.3, n = 16; Glu-CB1-KO: 85.3 ± 9.9, n = 8; p = 0.379, t test). A more detailed Sholl analysis was performed by plotting the number of intersections against the distance from the cell body. These results confirmed a general increase of dendritic complexity with a local significant range (240–290 μm; p < 0.05) in the apical part (Fig. 3D), but not in the basal dendritic segment (Fig. 3F). In these mice, the density of dendritic spines on excitatory, pyramidal neurons was also analyzed at the mid-apical portion of the apical dendrite (Fig. 4). Spine density was significantly increased in Glu-CB1-KO mice (Glu-CB1-WT: 1.70 ± 0.04, n = 31; vs Glu-CB1-KO: 1.89 ± 0.05, n = 29, p = 0.0036, t test; Fig. 4A).

Figure 3.
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Figure 3.

Morphology and Sholl analysis of Glu-CB1-KO and GABA-CB1-KO mice. A, Representative image of a DiI-labeled CA1 pyramidal neuron of Glu-CB1-KO mice. B, Representative image of a DiI-labeled CA1 pyramidal neuron of a GABA-CB1-WT mouse. Note that there was no significant difference between CA1 pyramidal neuron morphology of GABA-CB1-WT and Glu-CB1-WT mice. C, Representative image of a DiI-labeled CA1 pyramidal neuron of GABA-CB1-KO mice. Scale bar, 100 μm. D, Sholl analysis of Glu-CB1-KO mice versus WT littermate controls at apical dendrites of CA1 hippocampal neurons. E, Sholl analysis of GABA-CB1-KO mice versus WT littermate controls at apical dendrites of CA1 hippocampal neurons. F, Sholl analysis of Glu-CB1-KO mice versus WT littermate controls at basal dendrites of CA1 hippocampal neurons. G, Sholl analysis of GABA-CB1-KO mice versus WT littermate controls at basal dendrites of CA1 hippocampal neurons. Two-tailed Student's t test; *p < 0.05, **p < 0.01; error bars = SEM.

Figure 4.
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Figure 4.

Dendritic spine density in Glu-CB1-KO and GABA-CB1-KO mice. A, Spine density of CA1 hippocampal neurons from the mid-apical dendrites in Glu-CB1-KO mice versus WT littermate controls. B, Spine density of CA1 hippocampal neurons from the mid-apical dendrites in GABA-CB1-KO mice versus WT littermate controls. C, Individual examples of spines on mid-apical dendrites for all three genotypes. Statistics: t test; **p < 0.01; ***p < 0.001; error bars = SEM.

In contrast to these findings, the dendritic complexity of pyramidal cells in animals lacking the CB1 receptor on GABAergic neurons was significantly reduced in both apical (GABA-CB1-WT: 168.8 ± 22.5, n = 13; GABA-CB1-KO: 100.6 ± 11.6, n = 11; p = 0.019, t test) and basal dendrites (GABA-CB1-WT: 109.1 ± 9.7, n = 11; GABA-CB1-KO: 66.0 ± 10.9 n = 8; p = 0.009, t test). These results were supported by the Sholl analysis, showing decreased dendritic complexity locally in the basal and apical compartment (Fig. 3E,G). Significant differences were found between 230 and 440 μm in the apical dendrites (p < 0.05, t test). We also compared the density of dendritic spines at the mid-apical portion of the apical dendrite of GABA-CB1-KO animals and their WT littermates and found a significant reduction compared with control neurons (GABA-CB1-WT: 1.68 ± 0.04, n = 33; GABA-CB1-KO: 1.42 ± 0.04, n = 30; p = 0.00003, t test; Fig. 4B, cf. C for original pictures of mid-apical dendritic segments for all genotypes).

Discussion

Memory restricting molecules might function as checkpoints determining the occurrence, the rate and the specificity at which changes in neuronal connectivity are either implemented or prevented. Thereby, these memory controlling proteins might ensure that only relevant features of an event are stored (Abel et al., 1998; Zagrebelsky and Korte, 2014). This memory barrier is of special importance for animals in general and humans in particular, since it is a hallmark of higher cognitive function to select salient information from a noisy background by attending to and remembering only the most critical features (Zagrebelsky and Korte, 2014). It is a clear advantage for individuals to filter facts that are important for survival, rather than randomly storing all encountered information into their long-term memory. Moreover, it might be even more important to consolidate the most important facts and features and protect them from changes.

In this context, the endocannabinoid system might be of high relevance, as we show here the potential of the CB1 receptor to calibrate synaptic potentiation and dendritic morphology via the GABAergic and the glutamatergic system. LTP, an increase in synaptic strength resulting from synchronous or high-frequency stimulation of a neuronal pathway, is thought to be an electrophysiological correlate of learning and memory processes (Citri and Malenka, 2008). For the highly complex task of memory formation to properly operate, the brain has to maintain a well tuned activity; a refined and dynamic balance of excitation and inhibition (Hensch and Fagiolini, 2005). The CB1 receptor, present both in GABAergic and glutamatergic terminals, is particularly well positioned to accomplish this goal, as the endocannabinoids act as retrograde signals to tune neurotransmission in a negative feedback (Castillo et al., 2012).

Our study revealed that the lack of CB1 receptor in cortical glutamatergic neurons facilitates hippocampal LTP formation, whereas inactivation of CB1 receptor function in forebrain GABAergic neurons leads to diminished hippocampal LTP formation. How can this dichotomy be reconciled? It was shown that LTP induces the production of endocannabinoids (Stella et al., 1997; Castillo et al., 2012). They are synthesized at the postsynapse of activated glutamatergic neurons, and then travel in a retrograde manner to local presynaptic CB1 receptors, located on both glutamatergic and GABAergic terminals, which both can innervate the endocannabinoid-producing postsynaptic glutamatergic neuron. Upon binding of the endocannabinoids to the presynaptic CB1 receptor, neurotransmission is suppressed. In the hippocampus, short-term depolarization-induced suppression of glutamate transmission and GABA transmission was reported, in addition to a long-term suppression at the GABAergic synapse, called inhibitory LTD (Chevaleyre and Castillo, 2004; for review, see Castillo et al., 2012). Thus, the endocannabinoid system represents an important negative feedback mechanism at both glutamatergic and GABAergic synapses. This includes homosynaptic and heterosynaptic processes. We found evidence that in the Glu-CB1-KO (the CB1 receptor is deleted at glutamatergic terminals) the endocannabinoid-mediated transient suppression of neurotransmitter release will not be accomplished, although endocannabinoids are produced (due to the LTP stimulation protocol), but the target, the presynaptic CB1 receptor, is missing. Therefore, glutamate release and transmission are not suppressed. Consequently, excitability of glutamatergic neurons will be increased in Glu-CB1-KO, and LTP is facilitated compared with WT controls. In case of CB1 receptor deletion at the GABAergic terminal in GABA-CB1-KO, the postsynaptic glutamatergic neurons can produce endocannabinoids (due to the LTP stimulation protocol), but the presynaptic incoming GABAergic terminal lacks the CB1 receptor. Therefore, GABAergic inhibition is not suppressed, and the glutamatergic neurons are less excitable, leading to decreased LTP.

We did not only observe increased and decreased LTP in the Glu-CB1-KO and GABA-CB1-KO, respectively, compared with controls, but also alterations in spine density and dendritic morphology. The long-term loss of CB1 receptor in glutamatergic neurons, which started shortly after the last mitosis of the glutamatergic neurons during development (Mulder et al., 2008), appears to cause a hyperexcitable state in Glu-CB1-KO, leading to increased spine density and dendritic branching. Decreased spine density and dendritic branching was observed in the adult GABA-CB1-KO, where the loss of CB1 receptor also occurred during embryonic development (Berghuis et al., 2007). This suggests the presence of a hypoexcitable state in GABA-CB1-KO. The general hyperexcitable state of Glu-CB1-KO is well documented by the increased susceptibility to kainic acid-induced seizures (Monory et al., 2006). Using the same seizure model, GABA-CB1-KO, however, did not show altered seizure susceptibility. The reasons for this have not yet been clarified, but it is noteworthy that CB1 receptor is specifically expressed on cholecystokinin-positive interneurons.

The dichotomy of LTP and morphological phenotypes reported in this study is congruent with behavioral investigations, where we frequently observed opposite phenotypes. In Glu-CB1-KO, for example, object exploration (Häring et al., 2011), impulsivity (Lafenêtre et al., 2009), feeding response (Bellocchio et al., 2010), and active fear coping (Metna-Laurent et al., 2012) are decreased, whereas these behavioral outcomes are increased in GABA-CB1-KO. Based on these observations, such opposite phenotypes might be predicted for other behavioral or cellular experimental paradigms.

It is interesting to note that in global CB1 receptor-deficient mice (CB1-KO), where both the low to moderate CB1 receptor expression in glutamatergic pyramidal neurons and the very high CB1 receptor expression in GABAergic interneurons is missing, the phenotype is similar to that observed in Glu-CB1-KO. This has been reported for several behavioral phenotypes (Monory et al., 2006; Kamprath et al., 2009; Metna-Laurent et al., 2012), and facilitated hippocampal LTP (Bohme et al., 2000). In addition, CB1 receptor antagonism also facilitates hippocampal (Hoffman et al., 2007) and prefrontal cortex (Auclair et al., 2000) LTP. These observations suggest that CB1 receptor function in pyramidal cells “overwrites” the function of interneurons in terms of LTP generation. Although CB1 receptor expression in pyramidal cells is much lower than in the GABAergic cells, pyramidal cells greatly outnumber GABAergic interneurons (of which approximately one third expresses the CB1 receptor; Marsicano and Lutz, 1999; Steindel et al., 2013). Moreover, the computational potency of the pyramidal cells is much higher, too: they receive up to 30,000 inputs and target 10,000–60,000 other neurons themselves, whereas interneurons receive a maximum of 15,000 inputs and have up to 5000 synaptic targets (Buhl and Whittington, 2007). Consequently, CA1 pyramidal neurons receive more excitatory than inhibitory inputs. Finally, our recent data of CB1 receptor-mediated intracellular signaling showed that in the hippocampus agonist stimulation of CB1 receptor is more effectively transduced to G-protein activation in glutamatergic than in GABAergic cells (Steindel et al., 2013). Therefore, it is reasonable to argue that even with lower CB1 receptor expression levels, the higher connectivity of pyramidal neurons and the more effective signal transduction mechanisms of CB1 receptor in these cells lend this neuronal population a powerful control over local synaptic strength.

In the medial prefrontal cortex, CB1-KO mice showed decreased dendritic branching and dendritic length (Hill et al., 2011), and dendritic area and number of spines are decreased in the motor cortex (Ballesteros-Yáñez et al., 2007). In the basolateral amygdala, however, dendritic branching and length is increased in CB1-KO mice (Hill et al., 2011).

Both in vivo and in vitro applications of CB1 receptor agonists prevent the formation of hippocampal LTP (Paton et al., 1998; Hoffman et al., 2007; Fan et al., 2010), consistent with the memory impairments observed after systemic CB1 receptor agonist (e.g., THC) treatments in animals and humans. Chronic high-dose THC treatments lead to a strong downregulation of CB1 receptor in GABAergic neurons (Puighermanal et al., 2013), and to a decrease of CB1 receptor-mediated suppression of GABA, but not glutamate transmission (Hoffman et al., 2007). In congruency, the amnesic effects of acute high THC dose was not present in GABA-CB1-KO (Puighermanal et al., 2009), and chronic high-dose THC did not induce cognitive deficits in GABA-CB1-KO (Puighermanal et al., 2013). In the light that chronic THC downregulates CB1 receptor in GABAergic neurons in particular, one would expect a decrease of LTP formation, but an abolishment of LTP is observed (Hoffman et al., 2007). Thus, further investigations are needed to clarify this issue.

The well functioning endocannabinoid and CB1 receptor signaling helps to maintain a stable and balanced activity at neuronal and circuit levels, thereby representing an important homeostatic factor of the nervous system. Neuronal excitability relies on the summation of excitatory and inhibitory signals, both of which are controlled by endocannabinoids in the cerebral cortex and hippocampus. By deleting CB1 receptor from cortical/hippocampal glutamatergic or forebrain GABAergic neurons, an activity-related control mechanism of the release of glutamate or GABA, respectively, is removed. Consequently, the excitability of the whole system slightly shifts upward or downward, and reaches a new stable equilibrium state, a new allostatic set point, which is opposite to each other in Glu-CB1-KO and GABA-CB1-KO, but which both represent a nonfavorable and pathophysiological state, leading to cellular and behavioral alterations. Previous works have already reported the homeostatic role of the hippocampal endocannabinoid system after long-term neuronal silencing (Kim and Alger, 2010), and after strong neuronal activity (Bojnik et al., 2012). It remains to be investigated how the new allostatic set points in Glu-CB1-KO and GABA-CB1-KO are reached and regulated by possibly non-endocannabinoid-mediated mechanisms.

Footnotes

  • This work was supported in part by CRC 1080 to B.L. We thank Reinhard Huwe for sophisticated technical assistance, and Andrea Conrad, Anne Rohrbacher, and Anisa Kosan for genotyping of mice.

  • The authors declare no competing financial interests.

  • Correspondence should be addressed to Dr Martin Korte, TU Braunschweig, Divison of Cellular Neurobiology, Zoological Institute, Spielmannstr 7, D-38106 Braunschweig, Germany. m.korte{at}tu-bs.de

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Cannabinoid CB1 Receptor Calibrates Excitatory Synaptic Balance in the Mouse Hippocampus
Krisztina Monory, Martin Polack, Anita Remus, Beat Lutz, Martin Korte
Journal of Neuroscience 4 March 2015, 35 (9) 3842-3850; DOI: 10.1523/JNEUROSCI.3167-14.2015

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Cannabinoid CB1 Receptor Calibrates Excitatory Synaptic Balance in the Mouse Hippocampus
Krisztina Monory, Martin Polack, Anita Remus, Beat Lutz, Martin Korte
Journal of Neuroscience 4 March 2015, 35 (9) 3842-3850; DOI: 10.1523/JNEUROSCI.3167-14.2015
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  • cannabinoids
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