Abstract
Designer receptors exclusively activated by designer drug (DREADDs) are a novel tool with the potential to bidirectionally drive cellular, circuit, and ultimately, behavioral changes. We used DREADDs to evaluate memory formation in a hippocampus-dependent task in mice and effects on synaptic physiology in the dorsal hippocampus. We expressed neuron-specific (hSyn promoter) DREADDs that were either excitatory (HM3D) or inhibitory (HM4D) in the dorsal hippocampus. As predicted, hSyn–HM3D was able to transform a subthreshold learning event into long-term memory (LTM), and hSyn–HM4D completely impaired LTM formation. Surprisingly, the opposite was observed during experiments examining the effects on hippocampal long-term potentiation (LTP). hSyn–HM3D impaired LTP and hSyn–HM4D facilitated LTP. Follow-up experiments indicated that the hSyn–HM3D-mediated depression of fEPSP appears to be driven by presynaptic activation of inhibitory currents, whereas the hSyn–HM4D-mediated increase of fEPSP is induced by a reduction in GABAA receptor function. To determine whether these observations were promoter specific, we next examined the effects of using the CaMKIIα promoter that limits expression to forebrain excitatory neurons. CaMKIIα–HM3D in the dorsal hippocampus led to the transformation of a subthreshold learning event into LTM, whereas CaMKIIα–HM4D blocked LTM formation. Consistent with these findings, baseline synaptic transmission and LTP was increased in CaMKIIα–HM3D hippocampal slices, whereas slices from CaMKIIα–HM4D mice produced expected decreases in baseline synaptic transmission and LTP. Together, these experiments further demonstrate DREADDs as being a robust and reliable means of modulating neuronal function to manipulate long-term changes in behavior, while providing evidence for specific dissociations between LTM and LTP.
SIGNIFICANCE STATEMENT This study evaluates the efficacy of designer receptors exclusively activated by designer drug (DREADDs) as a means of bidirectionally modulating the hippocampus in not only a hippocampus-dependent task but also in hippocampal synaptic plasticity. This is the first study to evaluate the effects of DREADD-mediated inhibition and excitation in hippocampal long-term potentiation. More specifically, this study evaluates the effect of promoter-specific expression of DREADD viruses in a heterogenic cell population, which revealed surprising effects of different promoters. With chemogenetics becoming a more ubiquitous tool throughout studies investigating circuit-specific function, these data are of broad interest to the neuroscientific community because we have shown that promoter-specific effects can drastically alter synaptic function within a specific region, without parallel changes at the level of behavior.
- DREADDs
- long-term memory formation
- long-term potentiation
- object location memory
- object recognition memory
- theta-burst stimulation
Introduction
Chemogenetics is providing a novel method of controlling and studying neural function. Specifically, designer receptors exclusively activated by designer drug (DREADDs) allow for a unique approach to investigate circuit activity of specific brain regions, circuits, and their role in driving behavior. DREADDs are a family of mutated muscarinic acetylcholine receptors that provide reversible activation of G-protein-coupled receptor (GPCR) signaling cascades on application of an otherwise inert ligand, clozapine-n-oxide (CNO). These receptors have no other active ligand, whereas CNO has no other active receptor, creating a two-way exclusive approach for activating (Gs, Gq) or inhibiting (Gi) cellular activity. Gi, Gq, and Gs GPCR signaling cascades can be used for studying long-term cellular processing. As a result of this mechanism, DREADDs have diminished temporal resolution (because of both CNO metabolism and the temporal dynamics of GPCR) but are useful to study long-term processes. Activation of HM3Dq receptors induces internal Ca2+ waves through activation of PLC-dependent IP3 production. In this study, we use Gq-dependent signaling because of the known role activity-dependent Ca2+ plays in gene expression and learning and memory processes (Wu et al., 1995; Xia and Storm, 1997; Berridge et al., 1998; Wong et al., 1999; Pradhan and Liu, 2004). With regard to Gi signaling, it is important to note that there are four major signaling processes potentially being negatively affected: (1) cAMP; (2) GIRK channels; (3) β-arrestin; and (4) Ca2+ (Armbruster et al., 2007; Rogan and Roth, 2011; Ferguson and Neumaier, 2014). Previous work has shown that these designer receptors can be effectively used to bidirectionally modulate neuronal activity in a spatially and temporally specific manner (Armbruster et al., 2007; Alexander et al., 2009; Dong et al., 2010a,b; Rogan and Roth, 2011; Lee et al., 2014; Zhu and Roth, 2014), yet the majority of this work has been focused on aspects of motivated behaviors and addiction (Ferguson et al., 2011; Nair et al., 2013; Ferguson and Neumaier, 2014). There has been a relatively limited focus in using DREADDs to study memory consolidation (Garner et al., 2012; Robinson et al., 2014; Sano et al., 2014; Zhu et al., 2014; Fortress et al., 2015; Ishii et al., 2015; Tsuda et al., 2015; Yau and McNally, 2015). Moreover, the literature investigating the effect of DREADD modulation on long-term potentiation (LTP), a form of synaptic plasticity, is limited, especially in the hippocampus. However, as this tool becomes more ubiquitously used, it is critical to have a clearer understanding of how DREADDs induce changes to synaptic function that consequentially alter learning and memory performance. In this study, we used a DREADD approach to investigate their ability to regulate hippocampal LTP and hippocampus-dependent long-term memory (LTM).
The novel object recognition (NOR) series of tasks have been widely used to assess memory formation (for review, see Vogel-Ciernia and Wood, 2014). These tasks exploit rodents' inherent preference for novelty and can be used to study LTM formation in an incidental, noninvasive manner. Recent work, by our laboratory and others, has shown that the object location memory (OLM) task is a hippocampus-dependent task in rodents. Specifically, OLM requires the dorsal region of the CA1 subfield for LTM retrieval (Mumby, 2001; Mumby et al., 2002; Dere et al., 2007; Winters et al., 2008; Assini et al., 2009; Ennaceur, 2010; Barrett et al., 2011; Haettig et al., 2011; McQuown et al., 2011). Conversely, retrieval of memory in the object recognition memory (ORM) task has been shown to be hippocampus independent in rodents, relying on cortical structures (Haettig et al., 2011, 2013; McQuown et al., 2011; Vogel-Ciernia et al., 2013). Here, we show that DREADDs in the dorsal hippocampus can be used to bidirectionally modulate LTM formation in the OLM task but not the ORM task. Surprisingly, however, we discovered promoter-specific effects of expressing DREADDs in the hippocampus on hippocampal LTP.
Materials and Methods
Animals.
Eight-week old male C57BL/6 mice were purchased from The Jackson Laboratory. Animals were maintained in 12 h light/dark cycle with food and water provided ad libitum. All experiments were conducted according to the National Institutes of Health guidelines for animal care and use. Furthermore, experiments were approved by the Institutional Animal Care and Use Committee of the University of California, Irvine.
Stereotaxic surgeries.
For hSyn–HM3D experiments, animals received 1 μl of bilateral infusions to the dorsal hippocampus (ML, ±1.5 mm; AP, −2.0 mm; DV, −1.5 mm) of either AAV2.8—hSyn–GFP (n = 10–12; viral titer, 3.7 × 1012) control or AAV2.8–hSyn–HA–HM3D–IRES–mCitrine (n = 10–12; 2.3 × 1012). For hSyn–HM4D experiments, animals received similar infusions of either AAV2.8–hSyn–GFP (n = 10–12; 3.7 × 1012) control or AAV2.8–hSyn–HA–HM4D–IRES–mCitrine (n = 10–12; 4.2 × 1012). For CaMKIIα–HM3D experiments, animals received 1 μl of bilateral infusions to the dorsal hippocampus of either AAV2.8–CaMKIIα–GFP (n = 10–12; 5.6 × 1012) or AAV2.8–CaMKIIα–HA–HM3D–IRES–mCitrine (n = 10–12; 3.1 × 1012). For CaMKIIα–HM4D experiments, animals received similar infusions of either AAV2.8–CaMKIIα–GFP (n = 10–12; 5.6 × 1012) or AAV2.8–CaMKIIα–HA–HM4D—IRES–mCitrine (n = 10–12; 3.3 × 1012). Viruses were infused at a rate of 6 μl/h using a 30 gauge Neuros Hamilton syringe (product #65459-01) mounted to either a Harvard Apparatus Nanomite Syringe Pump (product #MA1 70-2217) or Leica Biosystems Nanoinjector Motorized f/Stereotaxics (product #39462901). All infusions used the Leica Microsystems Angle Two Stereotaxic system. Animals were allowed to recover for 7 d before handling. Behavioral testing and electrophysiological recordings began at day 21 after surgery, to allow for full expression of DREADD receptors. All viruses were purchased from the UNC Vector Core.
NOR tasks.
NOR tasks were performed as described previously (Vogel-Ciernia et al., 2013; Vogel-Ciernia and Wood, 2014). Briefly, animals were handled for 2 min over 5 consecutive days. Beginning on day 4 of handling, animals were habituated for 5 min to the OLM chamber for 6 consecutive days in the absence of the test objects. Animals then underwent a task training session. For HM3D experiments, animals were presented with two identical 100 ml beakers for 3 min. For HM4D experiments, animals were presented with these OLM training objects for 10 min. Animals were injected systemically 40 min before the training session with 3 mg/kg CNO (i.p., 0.3 mg/ml, 0.25% DMSO, 0.9% saline; made fresh daily). Animals were injected 40 min before behavior to allow for peak activation of DREADD receptors by CNO. After 24 h, LTM formation was tested for 5 min, in which the OLM training objects were presented, one of which in a novel location. After OLM testing, animals were allowed to recover for 5 d. Animals were then habituated to the ORM chamber for 6 consecutive days in the absence of the test objects. For HM3D experiments, animals were presented with two identical objects (either metal tins or glass candle holders) for 3 min. For HM4D, animals were presented with these ORM training objects for 10 min. Animals were injected systemically 40 min before the training session with 3 mg/kg CNO (i.p., 0.3 mg/ml, 0.25% DMSO, 0.9% saline; made fresh daily). Twenty-four hours later, animals' retention was tested for 5 min, in which one of the ORM training objects was replaced with a novel, previously unexplored object. Both the Training and Testing sessions were video recorded and hand scored by individuals blind to animal treatments. Videos were analyzed for total exploration of objects in addition to the discrimination index (DI) [(time spent exploring novel object − time spent exploring familiar object)/(total time exploring both objects)].
Tissue harvesting for immunohistochemistry and PCR.
For HM3D experiments, at least 72 h after testing, both HM3D and GFP animals received a subsequent dose of CNO, were returned to their home cage, and were killed after 70 min. For HM4D experiments, at least 72 h after testing, animals received a subsequent dose of CNO, were returned to their home cage for 40 min, were introduced to a novel context for 10 min, and were killed after 30 min. Because the HM4D virus was predicted to inhibit hippocampal activity, it would be difficult to interpret this by simply giving a subsequent CNO injection and returning the animals to the home cage. This would lead to a floor effect because there would be very limited basal hippocampal activity and would be difficult to parse out differences between GFP and HM4D animals. The introduction to the novel context was an attempt to induce hippocampal activity, which presumably the HM4D virus would have inhibited. For immunohistological and c-fos expression experiments, animals were dislocated cervically, and the brains were flash frozen in chilled isopentane. Flash-frozen 20 μm coronal sections and 500 μm, 1.0 mm2 punches of CA1 subfield of dorsal hippocampus were collected using a Leica CM 1850 cryostat at −18°C. To examine c-fos expression, RNA was isolated from aforementioned punches using RNeasy Mini kit (catalog #74106; Qiagen), and total RNA (50 ng) was reverse transcribed. cDNA was analyzed using Roche Light Cycler via Roche proprietary algorithms and REST 2009 software Pfaffl method (Pfaffl, 2001; Pfaffl et al., 2002). All values were normalized to GAPDH expression levels generated simultaneously. Both c-fos and gapdh primers were generated from the Roche Universal Probe Library; c-fos: left c-Fos primer, 5′-ggggcaaagtagagcagcta-3′; right c-fos primer, 5′-agctccctcctccgattc-3′; c-fos probe 46, atggctgc; gapdh: right GAPDH primer, 5′-atggtgaaggtcggtgtga-3′; left GAPDH primer, 5′-aatctccactttgccactgc-3′; GAPDH probe, tggcggtattgg (Rogge et al., 2013). The c-Fos probe is conjugated to FAM, whereas the GAPDH probe is conjugated to Lightcycler Yellow 555. For verification of viral expression in electrophysiological experiments, RNA was isolated using RNeasy Mini kit (catalog #74106; Qiagen), and total RNA was reverse transcribed. cDNA was analyzed using Bio-Rad MJ Mini-Personal Thermal Cycler for expression of either HM3D or HM4D viruses. Both HM3D and HM4D primers were generated from the Roche Universal Probe Library: hm3d: left HM3D primer, 5′-agtacaacctcgcctttgtttc-3′; right HM3D primer, 5′-atcggaggggctgtgtatc-3′; hm4d: left primer, 5′-tgaagcagagcgtcaagaag-3′; right HM4D primer, 5′-tcctccagcttgccattg-3′.
Immunohistochemistry.
To confirm expression of DREADD, flash-frozen sections were mounted to glass slides and stained for HA tag. Briefly, slices were fixed in 4% PFA for 10 min, washed twice in 0.1 m PBS for 5 min, and quenched in 1.5% H2O2 for 20 min. Tissue was permeated with a single 5 min wash in 0.1% Triton X-100 in PBS solution, washed in PBS three times for 5 min, and blocked for 1 h in 8% normal goat serum (NGS) and 0.3% Triton X-100 in 0.1 m PBS. After three 5-min PBS washes, slices were incubated overnight in an HA primary antibody solution containing anti-HA (1:1000, rat monoclonal, product #11867423001; Roche Diagnostics), 2% NGS, and 0.3% Triton X-100 in PBS at 4°C. Slices were washed three times for 5 min in PBS. Slices were then incubated in secondary solution (Alexa Fluor goat anti-rat 555 at 1:1000, 2% NGS, and 0.3% Triton X-100 in PBS) for 2 h at room temperature. After secondary incubation, slices were washed three times for 5 min in PBS, incubated in DAPI (1:15,000) in PBS, and washed in PBS three times. Slides were air dried and coverslipped with VectaShield Mounting Medium (product #H-1000). Tissue was imaged using Olympus Scanner VSBX61 to confirm expression of either HA-tagged DREADD receptor or GFP control.
Hippocampal slice preparation and recording.
Hippocampal slices were prepared as described previously (Barrett et al., 2011). Briefly, after isoflurane anesthesia, mice were decapitated, and the brain was quickly removed and submerged in ice-cold, oxygenated dissection medium containing the following (in mm): 124 NaCl, 3 KCl, 1.25 KH2PO4, 5 MgSO4, 26 NaHCO3, and 10 glucose. Transverse hippocampal slices (375 μm) through the mid-third of the septotemporal axis of the hippocampus were prepared using an FHC vibrating tissue slicer (model OTS-5000) before being transferred to an interface recording chamber containing preheated artificial CSF (aCSF) of the following composition (in mm): 124 NaCl, 3 KCl, 1.25 KH2PO4, 1.5 MgSO4, 2.5 CaCl2, 26 NaHCO3, and 10 glucose (maintained at 31 ± 1°C). Slices were perfused continuously with this solution at a rate of 1–1.5 ml/min while the surface of the slices were exposed to warm, humidified 95% O2/5% CO2. Recordings began after at least 1.5 h of incubation.
Field EPSPs (fEPSPs) were recorded from CA1b stratum radiatum using a single glass pipette (2–3 MΩ) filled with 2 m NaCl. Stimulation pulses (0.05 Hz) were delivered to Schaffer collateral–commissural projections using a bipolar stimulating electrode (twisted nichrome wire, 65 μm) positioned in CA1c. Current intensity was adjusted to obtain 50% of the maximal fEPSP response. In a separate set of experiments, antagonists of the AMPA and NMDA receptors were infused to block the negative-going fEPSP that is characteristic of excitatory transmission, leaving a positive-going evoked response that is blocked by picrotoxin (PTX), a GABAA receptor antagonist (Lambert et al., 1991; Arai et al., 1995). This response is referred to as the field inhibitory postsynaptic potential (fIPSP). Input/output curves were established before testing began to adjust current intensity that produced near maximal responses.
After establishing a 10–20 min stable baseline, test compounds (see below) were introduced into the infusion line by switching from control aCSF to drug-containing aCSF. To determine whether CNO treatment affects the threshold level of LTP in slices from hSyn–GFP control, hSyn–HM3D, and hSyn–HM4D infused mice, LTP was induced by delivering three theta bursts, with each burst consisting of four pulses at 100 Hz and the bursts themselves separated by 200 ms [i.e., theta burst stimulation (TBS)]. The stimulation intensity was not increased during TBS. Data were collected and digitized by NAC 2.0 Neurodata Acquisition System (Theta Burst) and stored on a disk.
Reagents.
For behavioral experiments, CNO was provided by the National Institute of Mental Health Chemical Synthesis and Drug Supply Program. For electrophysiological experiments, DNQX (Tocris Biosciences), d-(−)APV (Tocris Biosciences), and PTX (Sigma) were prepared fresh in aCSF, whereas a 10 mm stock solution of CNO (catalog #141704; Abcam) was dissolved in water and subsequently diluted to a working concentration (5 μm) in aCSF.
Data analysis.
All statistical tests were performed using GraphPad Prism 5 (GraphPad Software). Habituation data (distance traveled during individual habituation sessions), training, and testing videos were collected using ANY-maze behavioral analysis software. Habituation was analyzed using a two-way ANOVA to compare total distance traveled across the habituation sessions. Training and testing data were analyzed using a Student's t test to compare either exploration or DI between control and test animals. Slice physiology data in the text are presented as means ± SD, and fEPSP slope was measured at 10–90% fall of the slope. Data in figures on LTP were normalized to the last 10 min of baseline and presented as mean ± SE. CNO-induced changes on baseline measures were analyzed using a paired Student's t test to compare the pre-CNO with post-CNO infusion period and assessed as significant if p < 0.05. Baseline measures on paired-pulse facilitation and LTP were analyzed using a two-way ANOVA.
Results
hSyn–HM3D-dependent activation of the dorsal hippocampus can transform subthreshold training into LTM in OLM, but not ORM
To test whether activation of the dorsal hippocampus during a subthreshold training event can lead to LTM, hSyn–HM3D and hSyn–GFP infused animals received CNO 40 min before a 3 min training session. Human synapsin-1 is ubiquitously expressed throughout neurons, and viruses using the hSyn promoter have been shown to have high neuron-specific expression (Kügler et al., 2003). We have shown previously that a 3 min training period is insufficient to generate LTM tested at 24 h (Stefanko et al., 2009; Haettig et al., 2011, 2013; McQuown et al., 2011). After CNO-primed training, animals were tested for LTM in the OLM task (Fig. 1A). hSyn–HM3D animals showed a significant increase in DI compared with hSyn–GFP controls (t(19) = 3.387, p = 0.0031) during the test session, demonstrating that HM3D-mediated activation in the hippocampus transforms a subthreshold training period into robust LTM for object location (Fig. 1Bii). There were no differences between groups in training DI (Fig. 1Bi; t(19) = 0.6511, p = 0.5228), habituation (F(1,5) = 0.02544, p = 0.8749), training exploration (t(19) = 1.083, p = 0.2924), or test exploration (t(19) = 0.2446, p = 0.8094) in the OLM experiment (data not shown).
Bidirectional modulation of the dorsal hippocampus via hSyn–DREADDs affects LTM processes. A, Subthreshold training and testing paradigm for NOR tasks. B, Training and testing DIs of hSyn–GFP (white) and hSyn–HM3D (blue), shown as mean ± SEM. i, Mean DI for OLM training. ii, Mean DI for OLM testing. iii, Mean DI for ORM training. iv, Mean DI for ORM testing. C, Immunohistochemistry against DAPI (blue), GFP (green), and HA (red) in hSyn–GFP or hSyn–HM3D infused dorsal hippocampus. D, Normalized RT-qPCR measuring relative c-fos:GAPD expression in hSyn–GFP (white) and hSyn–HM3D (blue). E, Threshold training and testing paradigm for NOR tasks. F, Training and testing DIs of hSyn–GFP (white) and hSyn–HM4D (red), shown as mean ± SEM. i, Mean DI for OLM training. ii, Mean DI for OLM testing. iii, Mean DI for ORM training. iv, Mean DI for ORM testing. G, Immunohistochemistry against DAPI (blue), GFP (green), and HA (red) in hSyn–GFP or hSyn–HM4D infused dorsal hippocampus. H, Normalized RT-qPCR measuring relative c-fos:GAPD expression in hSyn–GFP (white) and hSyn–HM4D (red). For HM3D OLM and ORM experiments, n = 10 for hSyn–GFP and n = 11 for hSyn–HM3D. For HM4D OLM experiments, n = 11 for hSyn–GFP and n = 12 for hSyn–HM4D. For HM4D ORM experiments, n = 12 for hSyn–GFP and n = 12 for hSyn–HM4D. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.
After testing in the OLM task, animals underwent a similar training and testing paradigm in the ORM task (Fig. 1A). hSyn–HM3D animals tested for LTM in the ORM task after CNO-primed training showed no difference in DI compared with hSyn–GFP controls (t(19) = 0.05526, p = 0.9565), demonstrating that HM3D-mediated activation in the hippocampus does not affect LTM for object recognition (Fig. 1Biv). There were no measurable differences between groups in training DI (Fig. 1Biii; t(19) = 1.240, p = 0.23), habituation (F(1,5) = 0.4782, p = 0.4976), training exploration (t(19) = 0.9064, p = 0.3761), or test exploration (t(19) = 0.5898, p = 0.5623) in the ORM experiment (data not shown). Immunohistochemistry was used to verify expression of both hSyn–GFP and hSyn–HM3D viruses (Fig. 1C). To confirm in vivo expression and efficacy of hSyn–HM3D function, animals received a subsequent dose of CNO before being killed. RT-qPCR was used to measure c-fos expression in tissue collected from the dorsal hippocampus. Tissue expressing hSyn–HM3D showed a dramatic increase in normalized c-fos:GAPDH expression compared with hSyn–GFP control after a subsequent dose of CNO (Fig. 1D). These results suggest that activation of dorsal hippocampal neurons during training can transform a subthreshold learning event into LTM for OLM, a hippocampus-specific task, but not for ORM.
hSyn–HM4D-dependent inactivation can block LTM formation in OLM, but not ORM
To test whether dorsal hippocampus inactivation can block LTM formation during a training event that normally generates LTM, we administered CNO 40 min before 10 min training of hSyn–HM4D and hSyn–GFP infused animals. We have shown previously that a 10 min training period is sufficient for generating reliable LTM tested at 24 h (Stefanko et al., 2009; McQuown et al., 2011; Vogel-Ciernia et al., 2013; Vogel-Ciernia and Wood, 2014). Twenty-four hours after CNO-primed training, animals were tested for LTM in the OLM task (Fig. 1E). hSyn–HM4D animals showed a significant decrease in DI compared with hSyn–GFP controls (t(22) = 2.177, p = 0.0405) during the test session, demonstrating that HM4D-mediated inhibition of the dorsal hippocampus disrupts LTM for object location (Fig. 1Fii). There were no measurable differences between groups with regard to training DI (Fig. 1Fi; t(22) = 0.6071, p = 0.55), habituation (F(1,5) = 0.3796, p = 0.5441), training exploration (t(22) = 0.4126, p = 0.6839), or test exploration (t(22) = 1.934, p > 0.05) in the OLM experiment (data not shown).
After testing in the OLM task, animals underwent a similar behavioral paradigm for the ORM task (Fig. 1E). hSyn–HM4D animals tested for LTM in the ORM task after CNO-primed training showed no difference in DI compared with hSyn–GFP controls (t(21) = 0.2171, p = 0.8302), demonstrating that HM4D-mediated inhibition of the dorsal hippocampus does not affect LTM for object recognition (Fig. 1Fiv). There were no measurable differences between groups in training DI (Fig. 1Fiii; t(21) = 0.5423, p = 0.5933), habituation (F(1,5) = 0.3923, p = 0.5379), training exploration (t(21) = 1.034, p = 0.3127), or test exploration (t(21) = 0.9842, p = 0.3362) in the ORM experiment (data not shown). Together, these results indicate that inhibition of dorsal hippocampal activity during robust training, which is sufficient for LTM, can block the formation of LTM for OLM, but not for ORM. Expression of hSyn–GFP and hSyn–HM4D viruses was confirmed immunohistologically (Fig. 1G). However, to confirm in vivo efficacy of hSyn–HM4D function, animals received a subsequent dose of CNO, and RT-qPCR was used to measure c-fos expression in tissue collected from the dorsal hippocampus. Surprisingly, tissue expressing hSyn–HM4D also showed a dramatic increase in normalized c-fos:GAPDH expression compared with hSyn–GFP control after a subsequent dose of CNO (Fig. 1H). The cause of this c-fos induction is unknown and could be attributed to several factors, including interneuron function throughout the dorsal hippocampus. To more accurately characterize the hSyn-mediated effects on hippocampal function, we evaluated DREADD modulation electrophysiologically.
hSyn-dependent expression of DREADD receptors differentially affects synaptic transmission and LTP in hippocampal field CA1
We predicted that glutamatergic synaptic transmission involving the activation of Gq-mediated signaling pathways after CNO infusions would increase synaptic transmission and LTP in hippocampal slices expressing hSyn–HM3D DREADD receptors (Greget et al., 2011; Roggenhofer et al., 2013; Suárez et al., 2014). The opposite effects on synaptic transmission and LTP were predicted for CNO-induced excitatory synaptic transmission involving the activation of the inhibitory signaling pathways associated with Gi-mediated pathways regulated by hSyn–HM4D DREADD receptors (DeBock et al., 2003; Bailey et al., 2008). We tested these predictions in acute hippocampal slices prepared from hSyn–GFP control, hSyn–HM3D, and hSyn–HM4D infused mice. The effects of a 20 min infusion of CNO (5 μm) on fEPSPs and fIPSPs recorded in the apical dendrites of hippocampal region CA1 are summarized in Figure 2. CNO infusions had no detectable influence on fEPSP slope and amplitude in slices from hSyn–GFP animals (Fig. 2A,B).
Characterization of baseline synaptic transmission in hippocampal slices expressing hSyn-driven excitatory and inhibitory DREADD receptors. A–C, Hippocampal slices were prepared from adult hSyn-GFP control (gray circles; n = 6), hSyn–HM3D (green circles; n = 7), and hSyn–HM4D (red circles; n = 6) mice. The graphs show the mean ± SEM fEPSP slope (A), amplitude (B), and half-width plotted as a percentage change of baseline (C) in hippocampal slices treated for 20 min with 5 μm CNO, followed by a washout. D, Representative field responses collected from hSyn-GFP control, hSyn–HM3D, and hSyn–HM4D slices during the baseline recording period (pre-CNO) and 30 min after the end of CNO infusion (post-CNO). Calibration: 1 mV, 5 ms. E, i, Pharmacologically isolated fIPSP amplitudes (responses recorded in the presence of 20 μm DNQX and 100 μm APV, antagonists for AMPA and NMDA receptors, respectively) were evoked by stimulation of the Schaffer–commissural projections (pre-CNO, gray line, top trace) in slices prepared from hSyn-GFP control animals (n = 5 slices). Graph shows the mean ± SEM change in fIPSP amplitude as a percentage change of baseline. These responses were completely blocked by the GABAA receptor antagonist PTX (black line, top traces) and were unaffected by infusions of 5 μm CNO (blue line, top traces). Calibration: 0.1 mV, 10 ms. ii, In slices prepared from hSyn–HM3D mice (n = 7 slices), CNO infusions produced a delayed but significant increase in the fIPSP. In contrast, CNO infusions mimicked the effects of PTX by causing a dramatic decrease in the fIPSP and completely eliminating the field response 30 min after the end of the infusion period in slices (n = 5) from hSyn–HM4D mice. fIPSPs were unaffected by infusions of CNO in slices from hSyn-GFP controls (n = 5) during the recording session. Calibration: 0.1 mV, 10 ms.
Unexpectedly, CNO-treated slices from hSyn–HM3D mice caused a substantial decrease in the slope (−28 ± 14%, t(6) = 5.3, p = 0.0018) and amplitude (−17 ± 9%, t(6) = 4.4, p = 0.004) of the fEPSP relative to pre-CNO infusion (Fig. 2A,B,D). Field responses in slices from hSyn–HM4D mice also produced surprising results (Fig. 2A,B,D). CNO treatment caused a transient increase in slope, whereas the amplitude of the field response remained significantly elevated 20 min into the washout period relative to pre-CNO baseline values (9 ± 5%, t(5) = 4.5, p = 0.006). The sustained increase in fEPSP amplitude is likely attributable to the onset of a change in half-width that can cause changes in waveform as described below. The enhanced responses after CNO infusion in hSyn–HM4D slices was not accompanied by changes in paired-pulse facilitation when compared with pre-CNO treatment (F(1,27) = 0.32, p = 0.37), but a notable increase was observed at the shortest interval tested in hSyn–HM3D slices (F(1,21) = 5.6, p < 0.001). Thus, viral expression of DREADD receptors driven by the hSyn promoter in the hippocampus appears to recruit various cell types that mask the desired effects on synaptic transmission and suggests that CNO-induced decrease in synaptic transmission may involve changes in presynaptic function in hSyn–HM3D mice.
It is important to note that, unlike field responses recorded from hSyn–GFP control and hSyn–HM3D animals, the fEPSP in slices from hSyn–HM4D was accompanied by a significant broadening of the evoked potential (half-width, 45 ± 23%; Fig. 2C,D). This measure was significantly greater in hSyn–HM4D slices (t(5) = 4.5, p = 0.006) compared with slices from hSyn–HM3D (3 ± 8%, t(6) = 1.1, p = 0.31) and GFP control (1 ± 4%, t(9) = 0.77, p = 0.46) mice. The dramatic change in response waveform induced by CNO infusion in this group of animals suggests that the CNO-induced effect on field responses were not selective to glutamate receptors but rather included a GABAA receptor component that mediates feedforward inhibition. The fast feedforward IPSP disynaptically activated by the Schaffer–commissural fibers affects the amplitude and waveform of the fEPSP (Alger and Nicoll, 1982; Grover and Yan, 1999). Therefore, changes in these potentials could account for some, although not all, of the CNO-induced increases described above. To test this possibility, we pharmacologically isolated the fIPSP to test CNO-induced effects on inhibitory currents in hSyn–HM4D slices. As described in previous reports studying fIPSP in slices (Lambert et al., 1991; Arai et al., 1995), a PTX-sensitive response recorded in the presence of AMPA and NMDA receptor antagonists yields a positive-going fIPSP in slices from hSyn–GFP control mice as shown in Figure 2Ei. After a stable baseline recording, the fIPSP remained unchanged during the 20 min CNO infusion period in control slices. After a brief washout period, 100 μm PTX was added to the infusion line to confirm that the positive-going potential is the extracellular reflection of an IPSP. As illustrated, infusions of PTX completely eliminated the isolated fIPSP (Fig. 2Ei). We then tested CNO effects on the fIPSP in slices prepared from hSyn–HM3D and hSyn–HM4D mice (Fig. 2Eii). As anticipated, CNO-treated slices from hSyn–HM4D mice caused a dramatic decrease in the fIPSP beginning 10–15 min after the start of infusion and ultimately completely blocked fIPSP 30 min into washout. In contrast, bath applications of CNO did not cause an immediate, but rather a delayed, increase on fIPSP in slices from hSyn–HM3D mice. The increase in the fIPSP began 10–15 min into the washout period and reached a significant level by the end of the testing period (14 ± 12%, t(5) = 3.0, p = 0.03). CNO infusions did not alter fIPSPs in slices from hSyn–GFP controls during the duration of the experimental session. Collectively, these results suggest that, on one hand, the hSyn for the HM4D DREADD is increasing excitatory drive in hippocampal subfield CA1 by reducing inhibitory tone, whereas on the other hand, the HM3D DREADD is decreasing excitatory transmission on synaptic inputs to CA1 by increasing inhibitory tone.
We then were interested in determining whether the hSyn–DREADD receptor effects on synaptic transmission would remain consistent with predicted outcomes for changes in long-term plasticity. That is, would CNO-treated slices from hSyn–HM3D and hSyn–HM4D mice show impaired and enhanced LTP, respectively? Because the hSyn–HM4D animals appeared to have a reduction in GABAergic transmission (Fig. 2C,Eii), we used a subthreshold level of stimulation to induce LTP that consisted of a train of three theta bursts. Figure 3 summarizes the effects of theta burst-induced LTP in slices from hSyn–HM3D and hSyn–HM4D with respect to hSyn–GFP mice. TBS in CNO-treated slices from hSyn–GFP injected mice produced strong short-term potentiation that gradually stabilized over a 20 min period to a level that was 27 ± 12% above pre-TBS baseline 60 min after induction. This is in agreement with previous work showing that subthreshold levels of theta stimulation can produce lasting potentiation in mouse hippocampal slices (Vogel-Ciernia et al., 2013). In accordance with CNO-induced effects on baseline transmission in hSyn–HM4D mice, TBS caused a marked enhancement in LTP in this group of CNO-treated slices (55 ± 18%), whereas LTP failed to stabilize in CNO-treated slices from hSyn–HM3D mice (0 ± 14%). The enhancement in LTP in hSyn–HM4D slices is consistent with previous studies showing that blocking GABAA receptor activation enhances LTP in area CA1 (Chapman et al., 1998; Grover and Yan, 1999). The difference between groups of slices with CNO was significantly different 60 min after TBS (Fig. 3; F(2,15) = 73, p = 0.001). To confirm viral infusions in hSyn–HM3D and hSyn–HM4D slices, PCR on collected tissue was used with primers specific to either HM3D or HM4D receptors to rule out the possibility of crossed viral infusion, i.e., to validate that the inhibitory effect of the hSyn–HM3D virus was not attributable to being accidentally injected with hSyn–HM4D but is truly a characteristic of the hSyn–HM3D virus and vice versa. PCR with primers specific to the HM3D virus yielded bands only in tissue isolated from HM3D animals. Moreover, PCR with primers specific to the HM4D virus yielded bands only in tissue isolated from HM4D animals, thus precluding the possibility of virus cross-contamination (data not shown). Together, these studies strongly suggest that the hSyn promoter virus is transducing a global population of neurons in hippocampal region CA1 that regulate both excitatory and inhibitory synaptic inputs, thus making it difficult to interpret behavioral results on learning and memory.
Long-term plasticity changes associated with hSyn-driven DREADD receptor expression in adult hippocampal slices. The slope of the fEPSP was normalized to the mean of the last 60 responses (10 min) collected in the presence of 5 μm CNO before application of TBS (upward arrow). A single train of three theta bursts was effective in inducing stable potentiation in slices from hSyn-GFP control mice (n = 6) but produced a supranormal amount of potentiation in slices from hSyn–HM4D mice (n = 6). In contrast, theta burst-induced LTP decayed toward baseline values in slices from hSyn–HM3D (n = 6) mice. Inset, Traces collected during the last 5 min of CNO treatment (gray line) and 60 min after TBS (black, green, and red lines). Calibration: 1 mV, 5 ms.
Targeting the excitatory cell population via promoter specificity corrects for predicted outcome on synaptic transmission and long-term plasticity
Use of the CaMKIIα promoter has been shown to restrict viral expression to forebrain excitatory neurons (Mayford et al., 1996; Dittgen et al., 2004; White et al., 2011; Scheyltjens et al., 2015). Thus, we predicted that animals infused with the CaMKIIα-regulated HM3D and HM4D DREADD receptors would alter excitatory transmission in a positive and negative manner, respectively. In agreement with our hypotheses, bath-applied CNO (5 μm) caused a rapid increase in glutamatergic transmission beginning 5–10 min after the start of infusion in slices prepared from animals treated with CaMKIIα–HM3D (Fig. 4A). This effect was highly reproducible and consisted of a 20% increase over baseline by 30 min after the start of infusion. It is noteworthy to mention that there were no dramatic changes in the duration of the fEPSP (for comparison, see hSyn–HM4D; Fig. 2E,D). The opposite result was obtained with a 20 min application of CNO in slices from CaMKIIα–HM4D infused mice; CNO caused a significant drop, albeit more gradual, in fEPSP slope that was 15% below baseline values, whereas the synaptic responses collected from CaMKIIα–GFP control slices remained unchanged during the infusion and recording session. (Fig. 4A). The change in responses after CNO infusion of CaMKIIα–HM3D (F(1,21) = 3.7, p = 0.0003) was accompanied by changes in paired-pulse facilitation, as was in slices from the CaMKIIα–HM4D group (F(1,15) = 5.0, p < 0.001), suggesting that CNO-induced changes in baseline are mediating transmitter release kinetics.
Promotor-specific effects of CaMKIIα DREADD receptors on synaptic transmission and long-term plasticity. A, After stable baseline recordings, infusions of CNO (5 μm) produced a rapid increase in field responses collected from CaMKIIα–HM3D (n = 8) slices. In contrast, CNO infusions caused a marked decrease in fEPSP slope in slices from CaMKIIα–HM4D (n = 6) mice. Field responses from CaMKIIα–GFP (n = 6) slices were unaffected by CNO infusion. Inset, Representative traces collected during baseline recording (gray line) and 10 min after the end of CNO infusion (colored lines). Calibration: 1 mV, 5 ms. B, The slope of the fEPSP was normalized to the mean of the last 60 responses (10 min) collected in the presence of 5 μm CNO before application of TBS (upward arrow). A single train of three theta bursts delivered at the end of CNO infusion period was sufficient to induce short-term potentiation that then stabilized for the remainder of the recording period in control slices (CaMKIIα–GFP, n = 6) but not in slices from CaMKIIα–HM4D (n = 6). The same train applied at the end of the CNO infusion generated robust and stable LTP that was greater in magnitude in slices from CaMKIIα–HM3D (n = 8) mice than controls. Inset, Traces collected during the last 5 min of CNO treatment (gray line) and 60 min after TBS (black, green, and red lines). Calibration: 1 mV, 5 ms.
We then tested the effects of CNO on theta burst-induced LTP in CaMKIIα–HM3D and CaMKIIα–HM4D infused mice (Fig. 4B). Consistent with our previous predictions, the delivery of three theta bursts in the presence of CNO produced stable potentiation in slices from CaMKIIα–GFP mice (32 ± 7%) but generated robust LTP when delivered to slices with CaMKIIα–HM3D (59 ± 11%). The opposite was found in slices with CaMKIIα–HM4D in which potentiation failed to stabilize 30 min after induction and continued to drop toward baseline levels 60 min after TBS (7 ± 10%). The differences between groups were highly significant (F(2,17) = 81, p < 0.001). Post hoc tests revealed that LTP was increased reliably in slices from CaMKIIα–HM3D mice and reduced in CNO-treated slices from CaMKIIα–HM4D mice.
CaMKIIα–HM3D-dependent activation of dorsal hippocampus can transform subthreshold training into LTM in OLM, but not ORM
Although the effects of CaMKIIα–HM3D excitation of the dorsal hippocampus on LTP were as predicted, it was necessary to compare these hippocampal synaptic plasticity results with those of hippocampal learning. To test whether CaMKIIα–HM3D-mediated excitation of the dorsal hippocampus can transform a subthreshold training event into LTM, CaMKIIα–HM3D and CaMKIIα–GFP infused animals received CNO 40 min before a 3 min training session (Fig. 5A). Twenty-four hours after CNO-coupled training, animals were tested for LTM in the OLM task. CaMKIIα–HM3D animals showed a significant increase in DI compared with CaMKIIα–GFP controls (t(21) = 4.774, p = 0.0001), demonstrating that HM3D-mediated excitation in the hippocampus transforms a subthreshold training period into robust LTM for object location (Fig. 5Bii). There were no significant differences between groups in training DI (Fig. 5Bi; t(21) = 0.01476, p = 0.9884) or test exploration (t(21) = 1.298, p = 0.2082; data not shown). However, there was a modest, yet statistically significant, increase in CaMKIIα–HM3D animals' training exploration (7.891 ± 0.3704 s) compared with CaMKIIα–GFP (6.394 ± 0.4156 s; t(21) = 2.698, p = 0.0135). Furthermore, there was a significant difference in habituation (F(1,5) = 4.821, p = 0.0395). However, a Bonferroni's post hoc test revealed that the difference was only significant during day 1 of habituation and was no longer significant throughout days 2–6, suggesting that both groups of animals equally habituated to the OLM training context before training (data not shown).
Modulation of dorsal hippocampus via CaMKIIα–DREADDs leads to bidirectional changes in LTM processes. A, Subthreshold training and testing paradigm for NOR task. B, Training and testing DI of CaMKIIα–GFP (white) and CaMKIIα–HM3D (blue), shown as mean ± SEM. i, Mean DI for OLM training. ii, Mean DI for OLM testing. iii, Mean DI for ORM training. iv, Mean DI for ORM testing. C, Immunohistochemistry against DAPI (blue), GFP (green), and HA (red) in CaMKIIα–GFP or CaMKIIα–HM3D infused dorsal hippocampus. D, Threshold training and testing paradigm for NOR task. E, Training and testing DI of CaMKIIα–GFP (white) and CaMKIIα–HM4D (red), shown as mean ± SEM. i, Mean DI for OLM training. ii, Mean DI for OLM testing. iii, Mean DI for ORM training. iv, Mean DI for ORM testing. F, Immunohistochemistry against DAPI (blue), GFP (green), and HA (red) in CaMKIIα–GFP or CaMKIIα–HM4D infused dorsal hippocampus. For HM3D OLM and ORM experiments, n = 11 for CaMKIIα–GFP and n = 12 for CaMKIIα–HM3D. For HM4D OLM experiments, n = 12 for CaMKIIα–GFP and n = 11 for CaMKIIα–HM3D. For HM4D ORM experiments, n = 12 for CaMKIIα–GFP and n = 12 for CaMKIIα–HM3D. ***p ≤ 0.001.
After OLM testing, animals were subsequently trained and tested in the ORM task (Fig. 5A). CaMKIIα–HM3D and CaMKIIα–GFP animals tested for LTM in the ORM task 24 h after CNO-primed training showed no difference in DI compared with CaMKIIα–GFP controls (Fig. 5Biii; t(21) = 0.1542, 0.8789), with no measureable differences in habituation (F(1,5) = 3.276, p = 0.0846), training DI (Fig. 5Bii; t(21) = 0.1478, p = 0.8839), training exploration (t(21) = 1.079, p = 0.2928), or test exploration (t(21) = 1.079, p = 0.2928; data not shown). Expression of either CaMKIIα–GFP or CaMKIIα–HM3D in the dorsal hippocampus was confirmed immunohistologically (Fig. 5C). These results indicate that CaMKIIα–HM3D-mediated excitation of the dorsal hippocampus parallels the electrophysiological effects on LTP and is able to transform the subthreshold training event into LTM for the OLM task, but not the ORM task.
CaMKIIα–HM4D-dependent inactivation of the dorsal hippocampus can block LTM formation in OLM, but not ORM
To test whether CaMKIIα–HM4D-mediated inhibition of dorsal hippocampal neurons can disrupt LTM formation, we primed 10 min training of CaMKIIα–HM4D or CaMKIIα–GFP infused animals with CNO administration. Twenty-four hours after CNO-coupled training, animals were tested for LTM in the OLM task (Fig. 5D). CaMKIIα–HM4D animals showed a significant decrease in DI compared with CaMKIIα–GFP controls (Fig. 5Eii; t(21) = 5.490, p < 0.0001), with no significant differences in training DI (Fig. 5Ei; t(21) = 1.296, p = 0.2090), habituation (F(1,5) = 0.0569, p = 0.8136), training exploration (t(21) = 0.4539, p = 0.6545), or test exploration (t(21) = 0.5871, p = 0.5634; data not shown).
Animals were subsequently tested for LTM in the ORM task 24 h after CNO-primed training (Fig. 5D). CaMKIIα–HM4D animals showed no differences in testing DI compared with CaMKIIα-GFP controls (Fig. 5Eiv; t(22) = 0.1125, p = 0.9114). Moreover, there were no measurable differences in training DI (Fig. 5Eiii; t(22) = 0.07847, p = 0.9382), habituation (F(1) = 0.2521, p = 0.6206), training exploration (t(22) = 0.6564, p = 0.5184), or test exploration (t(22) = 0.8065, p = 0.4286; data not shown). Immunohistochemistry was used to confirm expression of CaMKIIα–GFP or CaMKIIα–HM4D (Fig. 5F). These results suggest that CaMKIIα–HM4D-mediated inhibition of the dorsal hippocampus parallels the electrophysiological effects on LTP and is able to prevent the formation of LTM for the OLM task, but not the ORM task.
Discussion
We implemented DREADDs to bidirectionally modulate the dorsal area of the hippocampus to understand the effect of DREADD-dependent modulation of hippocampus-dependent LTM and hippocampal LTP. Both hSyn–HM3D- and CaMKIIα–HM3D-mediated activation of the dorsal hippocampus was able to transform a subthreshold learning event into LTM for object location, but not object recognition. Additionally, hSyn–HM4D- and CaMKIIα–HM4D-mediated inactivation of the same region was able to block LTM formation for object location, but not object recognition. These behavioral results support previous findings in mice that the dorsal hippocampus appears to be particularly involved in OLM, but not object recognition (Mumby et al., 2002; Assini et al., 2009; Barrett et al., 2011; Haettig et al., 2011; Vogel-Ciernia et al., 2013; Vogel-Ciernia and Wood, 2014). Memory for object recognition appears to be more dependent on the perirhinal cortex (Winters et al., 2004; Winters and Bussey, 2005; Balderas et al., 2008) and the insular cortex (Bermudez-Rattoni et al., 2005; Balderas et al., 2008). However, the role of the hippocampus in memory for object recognition is more controversial (Mumby, 2001; Dere et al., 2007), because results tend to be dependent on various parameters and time between training and testing (Rossato et al., 2007; Balderas et al., 2008; Haettig et al., 2011).
It is possible that there are experimental sequence-dependent effects with regard to initial CNO administration for the OLM task and the subsequent acquisition of ORM in the protocol used in this study. Specifically, there may be long-lasting network effects of CNO administration during OLM training that may affect the training in the ORM task. However, given that both hSyn–HM4D and CaMKIIα–HM4D infused animals were able to acquire LTM for ORM after multiple administrations of CNO, this is unlikely. There were no detectable differences in motivation (exploration and total locomotion) during training sessions, and animals are able to acquire LTM for both OLM and ORM tasks. Thus, it is unlikely that CNO is affecting performance during the training sessions.
Most importantly in this study, we discovered promoter-specific effects of expressing DREADDs in the hippocampus on hippocampal LTP. The function of DREADDs on hippocampal LTP induced by TBS, a neuronal firing pattern occurring during exploratory behavior in rodents (Otto et al., 1991), had not been investigated previously. The hSyn–HM3D and hSyn–HM4D behavioral experiments led us to predict that hSyn–HM3D expression in the hippocampus would produce an enhancement in LTP, whereas an impairment in LTP was expected in slices from hSyn–HM4D-expressing mice. Surprisingly, we found the opposite. Application of CNO to slices expressing hSyn–HM3D in the dorsal hippocampus led to a significant decrease in amplitude of fEPSP and blocked TBS-induced LTP. In contrast, application of CNO to slices expressing hSyn–HM4D led to a significant increase in fEPSP amplitude and an enhancement of TBS-induced LTP. Our electrophysiological data suggest that CNO-induced depression of field responses in hSyn–HM3D mice may be driven by presynaptic activation of GABAA nerve terminals (Takahashi et al., 2007; Mathew and Hablitz, 2008), whereas hSyn–HM4D-mediated increase of fEPSP appears to be driven by depressing GABAA-mediated currents. These results suggest that results obtained with hSyn promoter-driven DREADDs should be interpreted carefully, particularly when used in adult ex vivo tissue with diverse local subpopulations.
CaMKIIα has been shown to drive expression in excitatory glutamatergic forebrain neurons. As expected, application of CNO to hippocampal slices led to decreased amplitude of fEPSP in CaMKIIα–HM4D-expressing slices, while leading to significantly increased amplitude in CaMKIIα–HM3D-expressing slices. Moreover, CNO priming in CaMKIIα–HM3D-expressing slices led to enhanced TBS-induced LTP, whereas TBS-induced LTP was blocked in CaMKIIα–HM4D-expressing dorsal CA1 slices. These results matched the predicted outcome of the experiments as suggested by the expression pattern of CaMKIIα and indicates that CaMKIIα may be a more ideal promoter for DREADD manipulations in the hippocampus, especially for excitatory neuronal manipulation.
The contrast between electrophysiological and behavioral results via hSyn-mediated altered signaling remains poorly understood. The disparate results may be primarily attributable to nonspecific viral-infected cells within local interneuron population that are known to tightly regulate activity of CA1 pyramidal cells in this region (Sik et al., 1995; Klausberger, 2009; Haettig et al., 2013). hSyn viral expression in local interneurons would explain the increase in c-fos expression after CNO exposure in hSyn–HM4D infused mice (Fig. 1H). Interestingly, numerous reports have shown a dissociation between hippocampal LTP and hippocampus-dependent LTM using genetically modified mice (Nosten-Bertrand et al., 1996; Gerlai et al., 1998; Migaud et al., 1998; Brakebusch et al., 2002; Kaksonen et al., 2002; Vaillend et al., 2004; Nakauchi et al., 2015). Several of these studies focus on elements typically associated with modification to NMDA/AMPA receptor function that produce an increase in stable LTP and a deficit in learning and memory tasks. Alternatively, Gerlai et al. (1998) proposed that an unregulated hyperexcitability induced by mutations to GluR2 led to enhanced LTP and impaired spatial learning. The commonality among several of these reports is that a subthreshold to threshold level of stimulation produced an increase in short-term potentiation followed by an enhancement of stable LTP relative to controls. The underlying mechanism responsible for a few of these mutant models was a decrease in inhibitory tone (Nosten-Bertrand et al., 1996; Vaillend et al., 2004). Consistent with these studies, we show that a subthreshold level of theta stimulation produced a marked increase in LTP in hSyn–HM4D infused mice relative to controls.
We also provide evidence that CNO infusions substantially reduced the amplitude of the fIPSP in hSyn–HM4D infused mice that was identical in time course and effect size after infusing the GABAA receptor antagonist PTX in control slices. These results strongly suggest that hSyn–HM4D virus interferes with inhibitory tone, which manifests as a notable increase in LTP and a decrease in learning and memory performance. In support of this conclusion, blocking GABAA receptors has been shown to enhance LTP by allowing greater postsynaptic depolarization and, thus, greater Ca2+ influx through NMDA receptors (Wigström and Gustafsson, 1983; Wigström and Gustafsson, 1985). Although at first glance, a reduction in stimulation threshold to raise the LTP ceiling for producing stable potentiation may seem an attractive approach to overcome disease-induced plasticity impairments associated with cognitive decline, neural network models of learning and memory that incorporate algorithms that can bidirectionally modify synapses predict that this type of animal model would ultimately cause saturation of synaptic function in a behaving animal and lead to negative consequences in processing information and its subsequent output to receiving cortical structures (Sejnowski, 1977; Bienenstock et al., 1982). Thus, animal models with similar phenotypes (enhanced LTP and impaired learning and memory) should be studied with caution. Although the goal of this study was not to determine the specific mechanism that interferes with the predicted outcome of hSyn–DREADD experiments, future studies may evaluate the particular interneuron population that leads to the counterintuitive effects on baseline synaptic signaling and hippocampal LTP.
Constructs implementing the hSyn promoter in other neuronal populations are likely to and already have been shown to yield the predicted effects on modulating cell activity (Chang et al., 2015; DiBenedictis et al., 2015; Gschwend et al., 2015; Huckstepp et al., 2015; Jovasevic et al., 2015; Pina et al., 2015; Robinson and Adelman, 2015; Sachs et al., 2015; Siegel et al., 2015; Ward et al., 2015; Koike et al., 2016). However, as DREADDs become more commonly used in regions with heterogeneous cell populations, it is critical to implement additional levels of specificity when using generic promoters, such as hSyn. In conjunction with transgenic animals expressing Cre under cell-type-specific promoters, Cre-dependent DREADDs (DIO–DREADD/FLEX–DREADD) can be used to investigate directly the role particular cell types have in various neural circuits and behaviors. These FLEX constructs are inactive until exposed to Cre recombinase, which correctly orients the vector to allow full expression. (Stamatakis and Stuber, 2012; Andero et al., 2014; Boender et al., 2014; Cai et al., 2014; Cassataro et al., 2014; Mahler et al., 2014; Robinson et al., 2014). By limiting DREADD expression with particular promoters, future work can exclusively modulate neural activity at the cell-type-specific level.
In summary, there are many new exciting developments in the use of chemogenetics for exploring the function of specific cell types in specific circuits at relatively precise time periods. In this study, we found promoter-specific effects with regard to the hSyn promoter. The results demonstrated that, although the behavioral results obtained were as predicted, the underlying synaptic physiology was not. Thus, promoter-specific chemogenetic approaches should be used with multiple levels of analysis to interpret the ultimate behavioral effects as correctly as possible. With regard to hippocampal function, we further demonstrated that HM3D-mediated activation is capable of transforming a subthreshold learning event into a robust LTM. Finally, the results continue to add to the literature in rodents demonstrating that OLM is hippocampus dependent, whereas ORM is hippocampus independent.
Footnotes
This work was supported by Department of Education Graduate Assistance in Areas of National Need Grants P200A120165 (A.J.L.) and P200A120165 (A.O.W.), Minority Biomedical Research Support–Initiative for Minority Student Development Grant GM055246 (A.J.L.), National Institute on Aging Grant AG000096-31 (J.K.), National Institute on Drug Abuse Grant F31DA038505 (A.O.W.), and National Institutes of Health Grants GM055246 (A.O.W.) and MH81004, MH101491, DA025922, DA036984, and DA031989 (M.A.W.). We acknowledge the Roth Laboratory, particularly Dr. Bryan Roth, at the University of North Carolina (UNC) at Chapel Hill, whose efforts developing DREADD constructs made this work possible, and UNC Vector Core, particularly Dr. R. Jude Samulski for providing viral vectors. We thank the National Institute of Mental Health Chemical Synthesis and Drug Supply Program for providing clozapine-n-oxide. Special thanks to Dr. John Guzowski of University of California, Irvine for use of the Olympus Scanner BX61VS for imaging.
- Correspondence should be addressed to Dr. Marcelo A. Wood, University of California, Irvine, Department of Neurobiology and Behavior, 301 Qureshey Research Building, Irvine, CA 92697. mwood{at}uci.edu
