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Articles, Development/Plasticity/Repair

BDNF Signaling Promotes Vestibular Compensation by Increasing Neurogenesis and Remodeling the Expression of Potassium-Chloride Cotransporter KCC2 and GABAA Receptor in the Vestibular Nuclei

Sophie Dutheil, Isabelle Watabe, Karina Sadlaoud, Alain Tonetto and Brahim Tighilet
Journal of Neuroscience 8 June 2016, 36 (23) 6199-6212; DOI: https://doi.org/10.1523/JNEUROSCI.0945-16.2016
Sophie Dutheil
1Department of Psychiatry, Yale University School of Medicine, New Haven, Connecticut 06519,
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Isabelle Watabe
2Laboratoire de Neurosciences Intégratives et Adaptatives, UMR 7260 Aix-Marseille Université-CNRS, Fédération de Recherche 3C, 13331 Marseille Cedex 03, France, and
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Karina Sadlaoud
2Laboratoire de Neurosciences Intégratives et Adaptatives, UMR 7260 Aix-Marseille Université-CNRS, Fédération de Recherche 3C, 13331 Marseille Cedex 03, France, and
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Alain Tonetto
3Fédération de Recherche Sciences Chimiques Marseille FR 1739, Pôle PRATIM, 13331 Marseille Cedex 03, France
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Brahim Tighilet
2Laboratoire de Neurosciences Intégratives et Adaptatives, UMR 7260 Aix-Marseille Université-CNRS, Fédération de Recherche 3C, 13331 Marseille Cedex 03, France, and
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Abstract

Reactive cell proliferation occurs rapidly in the cat vestibular nuclei (VN) after unilateral vestibular neurectomy (UVN) and has been reported to facilitate the recovery of posturo-locomotor functions. Interestingly, whereas animals experience impairments for several weeks, extraordinary plasticity mechanisms take place in the local microenvironment of the VN: newborn cells survive and acquire different phenotypes, such as microglia, astrocytes, or GABAergic neurons, whereas animals eventually recover completely from their lesion-induced deficits. Because brain-derived neurotrophic factor (BDNF) can modulate vestibular functional recovery and neurogenesis in mammals, in this study, we examined the effect of BDNF chronic intracerebroventricular infusion versus K252a (a Trk receptor antagonist) in our UVN model. Results showed that long-term intracerebroventricular infusion of BDNF accelerated the restoration of vestibular functions and significantly increased UVN-induced neurogenesis, whereas K252a blocked that effect and drastically delayed and prevented the complete restoration of vestibular functions. Further, because the level of excitability in the deafferented VN is correlated with behavioral recovery, we examined the state of neuronal excitability using two specific markers: the cation-chloride cotransporter KCC2 (which determines the hyperpolarizing action of GABA) and GABAA receptors. We report for the first time that, during an early time window after UVN, significant BDNF-dependent remodeling of excitability markers occurs in the brainstem. These data suggest that GABA acquires a transient depolarizing action during recovery from UVN, which potentiates the observed reactive neurogenesis and accelerates vestibular functional recovery. These findings suggest that BDNF and/or KCC2 could represent novel treatment strategies for vestibular pathologies.

SIGNIFICANCE STATEMENT In this study, we report for the first time that brain-derived neurotrophic factor potentiates vestibular neurogenesis and significantly accelerates functional recovery after unilateral vestibular injury. We also show that specific markers of excitability, the potassium-chloride cotransporter KCC2 and GABAA receptors, undergo remarkable fluctuations within vestibular nuclei (VN), strongly suggesting that GABA acquires a transient depolarizing action in the VN during the recovery period. This novel plasticity mechanism could explain in part how the system returns to electrophysiological homeostasis between the deafferented and intact VN, considered in the literature to be a key parameter of vestibular compensation. In this context, our results open new perspectives for the development of therapeutic approaches to alleviate the vestibular symptoms and favor vestibular function recovery.

  • BDNF
  • GABA
  • gliogenesis
  • KCC2
  • neurogenesis
  • vestibular compensation

Introduction

Cell proliferation in the adult mammal sometimes occurs after lesions or pathological injury to the CNS, but these immature new cells rarely provide any functional benefit. In addition, it is rare for newly generated cells to differentiate into neurons in the adult mammal without the assistance of drug treatment and it is even rarer to observe a complete recovery of function within a few weeks. However, this unusual generative and restorative process, which is more typical of the developing brain, happens after vestibular loss in the adult cat's brain.

Sudden, unilateral vestibular lesion abruptly shuts down the spontaneous resting activity of the vestibular nuclei (VN) neurons of the brainstem on the damaged side, resulting in an imbalance with the intact contralateral VN neurons (Zennou-Azogui et al., 1993; Ris et al., 1995; Ris and Godaux, 1998; Vidal et al., 1998). In a wide variety of species, this imbalance produces diverse symptoms such as balance and gait problems, oscillopsia, spontaneous nystagmus, vegetative disorders including nausea, and other severe impairments (Cass and Goshgarian, 1990; Lacour, 2006). These symptoms are described by patients as extremely unpleasant, but in most cases, they last a few weeks and ameliorate over time in a process called vestibular compensation (Smith and Darlington, 1991; Dieringer, 1995; Vidal et al., 1998; Lacour and Tighilet, 2010). This functional recovery involves sensory and behavioral substitution processes, which contribute to the recovery of dynamic functions, and plasticity mechanisms sustaining the restoration of activity between the deafferented and the intact VN, which leads to the recovery of the static functions of the vestibular system (Lacour and Tighilet, 2010). Many neuroplasticity mechanisms have been described to support vestibular compensation, such as changes in transcription factors expression, alteration in neurotransmitter and hormonal systems, changes in neurotrophins and their receptors, modifications of the density of membrane receptors, and astrocyte and microglial reactions (Lacour and Tighilet, 2010). Interestingly, we were the first to demonstrate that a unilateral vestibular neurectomy (UVN) induces a reactive neurogenesis in the VN of adult cats (Tighilet et al., 2007). In a follow-up study, continuous intracerebroventricular administration of an antimitotic drug immediately after UVN completely blocked the reactive cell proliferation and delayed 3-fold the recovery of posturo-locomotor functions (Dutheil et al., 2009), indicating that UVN-induced newborn cells contribute to behavioral recovery. In another study that confirmed previous results, we reported that GABA is a major regulator of vestibular compensation, not only by coordinating cellular events—including the different steps of reactive neurogenesis—but also by regulating the speed of recovery of posturo-locomotor functions (Dutheil et al., 2013).

Brain-derived neurotrophic factor (BDNF), a member of the neurotrophin family of proteins, has been shown to promote neurite outgrowth, cell survival, differentiation, migration, and activity-dependent neuroplasticity in the CNS and peripheral nervous system (Greenberg et al., 2009; Park and Poo, 2013). In the context of adult hippocampal neurogenesis, BDNF is known to influence neuronal activity through GABA-mediated effects (Lee et al., 2002; Sairanen et al., 2005; Bergami et al., 2008; Waterhouse et al., 2012). Interestingly, BDNF, through its associated tyrosine kinase B receptor (TrkB), is also necessary for the normal development of the vestibular system (Ernfors et al., 1994; Schimmang et al., 1995; Fritzsch et al., 1997; Lucas et al., 2014) and for fine vestibular compensation in adult mammals (Bolger et al., 1999; Maingay et al., 2000; Li et al., 2001). Based on this evidence, the current study sought to determine whether, after vestibular injury, BDNF-TrkB signaling affects the following: (1) the time course of recovery of vestibular functions, (2) postlesion plasticity mechanisms in the deafferented VN, and (3) the expression level of two specific markers of excitability, the GABAA receptor (GABAAR) and the cation-chloride cotransporter KCC2.

Materials and Methods

Ethics statement

All experiments were performed in accordance with the National Institutes of Health's Guide for Care and Use of Laboratory Animals (NIH Publication no. 80-23) revised in 1996 for the UK Animals (Scientific Procedures) Act of 1986 and associated guidelines or the Policy on Ethics approved by the Society for Neuroscience in November 1989 and amended in November 1993. Every attempt was made to minimize both the number of animals used and their suffering in this experiment. We selected only the most important post-UVN time delay according to the findings of our previous studies. Animals were housed in a large, confined space with normal diurnal light variations and free access to toys, water, and food.

Surgery and drug protocol

Adult male cats weighing between 4 and 5 kg were anesthetized with ketamine (20 mg/kg, i.m.; Rhône Poulenc), received an analgesic (tolfenamic acid, 4 mg/kg, i.m.; Vetoquinol) and were kept at physiological body temperature using a blanket. The vestibular nerve was sectioned on the left side at the postganglion level to leave the auditory division intact after mastoidectomy, partial destruction of the bony labyrinth, and surgical exposure of the internal auditory canal (Xerri and Lacour, 1980). Animals were maintained under antibiotics for 7 d and the same analgesics for 3 d. The classical postural, locomotor, and oculomotor deficits displayed by the animals in the days after nerve transection were used as criteria indicating the effectiveness of the vestibular nerve lesion. Completeness of vestibular nerve section had been assessed by histological procedures in previous studies (Lacour et al., 1976). For the implantation and use of osmotic minipumps containing saline, BDNF, or TrK antagonist (K252a), concentrations of BDNF and K252a were selected with reference to studies showing the effect of in vivo infusion of BDNF on neurogenesis (Pencea et al., 2001) and vestibular compensation (Maingay et al., 2000). K252a is a BDNF antagonist that has a high affinity for the TrkB receptor and belongs to the K252 family of alkaloid toxins, which are protein kinase inhibitors. BDNF and K252a (Sigma-Aldrich) were diluted to the required concentrations using artificial CSF containing the following (in mm): 124 NaCl, 5 KCl, 1.2 KH2PO4, and 1.3 MgSO4, tested for pH, and adjusted to a pH 7.0, if necessary. Subcutaneous minipumps (Alzet; flow rate 2.5 μl/h for 30 d) were filled with the solution to infuse as described previously (Dutheil et al., 2009, 2013).

Study design

To determine the effects of BDNF or K252a infusion after UVN on the different steps of reactive neurogenesis at the cellular level and on the time course of the cats' recovery at a behavioral level (see Fig. 1A), we studied 10 groups of male adult cats. Based on our previous findings regarding the temporal expression pattern of neurogenesis in the VN (Tighilet et al., 2007), two main time points were chosen: (1) 3 d after UVN to allow us to examine the cell proliferation at its highest expression and (2) 30 d after UVN while BrdU was injected 3 d after UVN to study the survival and the differentiation of the proliferating cells in reaction to the lesion. More specifically, the different groups were as follows: (1) sham group: the animals (Group 1, n = 4) were submitted to anesthesia and the same surgical approach as UVN without sectioning the nerve and immediately received a continuous 0.9% NaCl infusion. These animals were killed 30 d later; (2) UVN-NaCl group: animals underwent UVN with continuous NaCl infusion and then received a BrdU injection (200 mg/kg, i.p.) and killed either 3 d later, when cell proliferation reached a peak (Group 2, n = 4), or 30 d later to study the survival and the differentiation of the proliferating cells (Group 3, n = 4); (3) UVN-BDNF group: animals underwent UVN with continuous BDNF infusion, received a BrdU injection, and were killed 3 d later (Group 4, n = 4) or 30 d later (Group 5, n = 4); and (4) UVN-K252a group: animals underwent UVN with continuous K252a infusion, received a BrdU injection, and were killed 3 d later (Group 6, n = 4) or 30 d later (Group 7, n = 4). The two postlesion survival periods (days 3 and 30) were selected on the basis of our previous data (Tighilet et al., 2007). Concerning the behavioral investigations, 12 UVN cats were used for this study and received continuous infusion of NaCl (Group 8, n = 4), BDNF (Group 9, n = 4), or K252a (Group 10, n = 4) after the surgery. Because the Alzet minipump was filled with enough drug to be delivered continuously for 30 d, animals undergoing behavioral investigation stopped getting NaCl, BDNF, or K252a delivered after 1 month and continued being tested until they reached their pre-UVN performance or 160 d after UVN for the UVN-K252a group (Group 10).

Cellular investigations

Tissue preparation.

As described in a previous study (Dutheil et al., 2013), BrdU (10 mg/ml; Sigma-Aldrich) was dissolved in a solution of NaCl 0.9% heated to 56°C and injected into animals (200 mg/kg). BrdU doses were not likely to generate side effects, but were sufficient to mark the cells in S-phase synthesizing DNA. Before BrdU administration, the cats in each group were deeply anesthetized with ketamine dihydrochoride (20 mg/kg, i.m.; Merial). The last hour before they were killed, animals were deeply anesthetized with ketamine dihydrochloride (20 mg/kg, i.m.; Merial) and then perfused with 0.9% NaCl (1 L per animal), followed by freshly prepared paraformaldehyde 4% solution (2 L per animal). After removal from the skull, brains were cut into several blocks containing the VN. Blocks were rapidly frozen with dry ice and stored at −80°C. Coronal sections (40 μm thick) were cut in a cryostat (Leica) for immunochemistry.

Immunochemistry.

Immunochemical labeling was performed according to previously validated protocols (Brezun and Daszuta, 2000; Tighilet et al., 2007). The differentiation of the newly generated cells was analyzed in the UVN-NaCl, UVN-BDNF, and UVN-K252a groups injected with BrdU 3 d after UVN and killed after 1 month. Double immunofluorescence-stained sections incubated with BrdU and one of four antibodies were used: NeuN, a postmitotic neuronal nuclei marker expressed in most neurons; the glial fibrillary acidic protein (GFAP), a specific type of intermediate filament protein used as an astrocyte marker; IBA1, an ionized calcium-binding adapter molecule 1 that is specific to microglia and macrophages, but not cross-reactive with neurons and astrocytes; GAD 67, the enzyme that catalyzes the decarboxylation of glutamate to GABA and expressed in GABAergic neurons; and ChAT, a transferase enzyme responsible for the synthesis of the neurotransmitter acetylcholine and expressed in cholinergic neurons. Each antibody was processed sequentially, the differentiation marker detection first and then the BrdU labeling. For fluorescence labeling, sections were incubated with a secondary antibody coverslipped in Mowiol. Differentiation of the newly generated cells was analyzed with double-labeling analysis performed using confocal imaging with a Zeiss LM 710 NLO laser-scanning microscope equipped with a 63x/1.32 numerical aperture (NA) oil-immersion lens. The fields of view were then examined by confocal microscopy, and 1 μm-step Z -series were obtained. For GABAAR and KCC2 immunohistochemistry, sections were incubated overnight at 22°C in a mixture of affinity-purified rabbit KCC2-specific polyclonal antibody and the monoclonal mouse antibody against GABAARβ 2,3. Labeling was revealed with a mixture of donkey Cy3-conjugated rabbit-specific antibody and donkey Alexa Fluor 488-conjugated mouse-specific antibody and coverslips were mounted with a gelatinous aqueous medium. The patterns of immunolabeling were analyzed with a laser scanning confocal microscope (Zeiss LSM 710 META) at high magnification (Plan Apochromat 63× 1.4 NA oil-immersion objective). The optimal antibody dilutions and staining procedures are described in Table 1.

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Table 1.

Antibodies and methods of detection

Cell-counting method and quantification of GABAAR and KCC2 immunoreactivity

Cell counts were performed according to a previously validated protocol (Dutheil et al., 2009, 2011). The VN were identified through Berman's stereotaxic atlas (Berman, 1968). BrdU+, GFAP+, IBA1+, ChAT67+, and GAD67+ cells were quantified on both sides (left/right: sham-operated cats; ipsilateral/contralateral: UVN-lesioned cats) for each VN (medial, inferior, superior, and lateral VN: MVN, IVN, SVN, and LVN, respectively) from selected serial frontal sections collected from the dorsal (5.2) to the caudal (12.1) part of the brainstem and depending on the size and the rostrocaudal length of each VN. The cell count was done with a Nikon microscope (Eclipse 80i) equipped with a motorized X-Y-Z-sensitive stage and a video camera connected to a computerized image analysis system (Mercator; Explora Nova). The total number of immunolabeled cells was estimated using the optical fractionator method (West et al., 1991). For quantification of KCC2 and GABAAR immunohistochemistry, double fluorescence labeling was captured using frame-channel mode to avoid any cross-talk between the channels. Each optical section resulted from two scanning averages. Excitation of the fluorochromes was performed with an argon ion laser set at 488 nm and a helium/neon laser set at 575 nm. At high magnification, we only scanned the lateral VN, which is constituted by giant neurons (100 μm average) with visible nuclei and, for each soma, we digitized stacks of 1-μm-thick optical sections. We overlayed the internal and external borders, and digitized intensity measurements of KCC2 and GABAAR labeling using ZEN 2012 software. This method of quantification has been validated in other studies (Boulenguez et al., 2010; Sadlaoud et al., 2010; Bos et al., 2013).

Behavioral investigations

Spontaneous nystagmus recovery.

The day after UVN, each cat of the behavioral cohort was placed on an apparatus with its head fixed, thus maintaining the horizontal semicircular canals in the horizontal plane. The frequency of the horizontal spontaneous nystagmus was measured in the light as the number of quick phase beats toward the contralateral side relative to UVN in 10 s (five repeated measures per animal per sampling time) with a video camera (Sony HDV).

Posture recovery.

Posture deficits and recovery were evaluated by measuring the surface delimited by the four legs of the cats while they were standing erect at rest on a device with a graduated transparent floor that allowed them to be photographed from underneath. Five repeated measurements were done for each cat tested at each preoperative and postoperative time so that each animal acted as its own control. An average was calculated for each experimental session by an image analysis system (canvas, 9TM; Deneba Software).

Equilibrium function recovery.

The rotating beam test was performed according to standard published procedures used in our laboratory (Xerri and Lacour, 1980; Tighilet et al., 2006). Cats were first conditioned to cross over the immobile beam few weeks before surgery and were rewarded by a small piece of food placed in the target compartment. As a rule, rotation velocity of the beam was progressively increased after four consecutive trials without fall. Equilibrium function was quantified by measuring the highest speed of beam rotation that did not induce a fall and was called maximal locomotor balance performance (MaxP). For more details, see Dutheil et al. (2009, 2013).

Statistical analysis of the behavioral and cellular data.

Statistical analysis was done with ANOVA to test for changes at the different postlesion delays in the spontaneous nystagmus, the support surface, and the maximal equilibrium performance of the cats. Results were considered significant at p < 0.05. The statistical analyses of the cellular data were also evaluated by ANOVA to test the effects of the group (UVN-NaCl, UVN-BDNF, or UVN-K252a), the structure (MVN, IVN, LVN, SVN), the side (deafferented vs intact) and the postoperative time on BrdU, IBA1, GFAP, ChAT, GAD67, KCC2, and GABAARs immunostainings to determine whether there were any interactions between these variables. ANOVA was followed by post hoc analysis with the Scheffé test and multicomparison Fisher's test (StatView II; SAS Institute).

Results

BDNF signaling is required for behavioral recovery after UVN

Unilateral vestibular damage results in a constellation of heterogeneous symptoms in mammals: ocular nystagmus (fast phase directed to the intact side), head tilt toward the side of the lesion, postural asymmetry, enhancement of the support surface, and falling to the lesioned side in the first postlesion week in cats. To determine whether BDNF signaling modulates behavioral recovery of animals after UVN, we infused NaCl, BDNF, or K252a intracerebroventricularly for longitudinal behavioral analyses of Groups 8, 9, and 10 (see “Study design” section and Fig. 1A for more information).

Figure 1.
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Figure 1.

After UVN, behavioral recovery, reactive cell proliferation, and cell survival are altered by BDNF and K252a infusions in the deafferented VN. A, Experimental protocol to study the effects of continuous infusion in the fourth ventricle of BDNF or K252a (a TrK receptor blocker) at the cellular level, accompanied by behavioral analyses (n = 4 animals per group). B, Curves illustrating the time course of disappearance of horizontal spontaneous nystagmus frequency for each group of cats at different postoperative days. C, Curves indicating the mean postoperative recovery of the support surface after vestibular damage. D, Curves indicating the mean postoperative recovery of the equilibrium function after vestibular damage. The MaxP is defined as the highest beam rotation speed that did not lead to a fall on four consecutive crossings. The curves are expressed in percentage of the preoperative maximal performance as a function of the postoperative time in days.

Nystagmus

Unilateral vestibular damage produces a spontaneous nystagmus. BDNF infusion led to a decrease in the number of nystagmus beats at day 3 compared with K252a- and NaCl-treated cats (vehicle: 10.25 beats/10 s, BDNF: 3 beats/10 s, K252a: 13.5 beats/10 s, p < 0.0001; Fig. 1B). Overall, the total disappearance of spontaneous horizontal nystagmus was faster for the UVN-BDNF group (4 d) than for the vehicle group (8 d) and the K252a-treated group (13 d) (p < 0.0001).

Posture function recovery

Unilateral vestibular damage leads to an increased support surface delimited by the four paw pads of four-footed animals. This parameter reflects a good estimation of postural stability and recovery. As a rule, the surface was ∼50–100 cm2 in cats before UVN and significantly increased in the days after unilateral vestibular lesion. Return to pre-UVN control values, normalized to 1, was faster when animals were infused with BDNF (22 d) rather than NaCl (48 d). K252a drastically delayed the return to control values: cats of this group recovered preoperative values only after 98 d (Fig. 1C). Two-way ANOVA established significant effects depending on the groups (p < 0.0001), the postlesion time (p < 0.0001), and the interaction between these two factors (p < 0.0001).

Locomotor balance recovery

The rotating beam experimental device test provides a reliable behavioral measure of locomotor balance function consistent with the time lag necessary for the progressive disappearance of the posturo-locomotor vestibular symptoms experienced after vestibular damage. The role of BDNF in the posturo-locomotor recovery was tested in cats exposed to pre-UVN rotating beam training. Congruent with the data of the posture function and the nystagmus, BDNF improved substantially the dynamic locomotor balance of the animals, which were able to cross the rotating beam at their maximal performance after only 18 d after UVN, whereas NaCl animals reached the same performance at 36 d. In contrast, cats infused with K252a could not walk on the beam rotating at the lowest speed until 66 d after UVN and finally reached a plateau of 70% of their MaxP at 160 d (Fig. 1D). Two-way ANOVA showed significant effects depending on the drug infused (p < 0.0001), the postlesion time (p < 0.0001), and the interaction between these two factors (p < 0.0001).

BDNF expression influence cell proliferation and survival of new cells in the VN after vestibular damage

Based on our previous studies (Dutheil et al., 2009, 2013), exogenous NaCl, BDNF or K252a was continuously delivered (intracerebroventricularly via minipump) immediately after UVN in adult cats to examine postlesion plasticity mechanisms in the VN. We first assessed reactive cell proliferation in the VN (Groups 1, 2, 4, and 6 as described in the “Study design” section) by measuring the number of cells that incorporated the DNA synthesis marker BrdU after short-term infusion (at day 3, when cell proliferation has been described to reach a peak in this model of lesion; Tighilet et al., 2007). Results showed that BDNF increases the number of BrdU+ cells by ∼4-fold compared with the UVN-NaCl group (p < 0.0001; Fig. 2A,B). In contrast, intracerebroventricular administration of the high-affinity TrK receptor blocker K252a caused an ∼2-fold decrease of the BrdU+ cells in all of the deafferented VN group compared with the UVN-NaCl group (p < 0.0001) and, as described previously, we did not observe a significant number of BrdU+ cells in sham animals.

Figure 2.
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Figure 2.

BDNF treatment alters vestibular cell proliferation and cell survival. A, Photomicrographs showing BrdU immunostainings in the deafferented MVN 3 or 30 d after UVN with continuous drug infusion in the fourth ventricle (NaCl, BDNF, or K252a). B, Histograms comparing the mean values (SEM) of the number of BrdU+ cells that incorporated BrdU at day 3 afater UVN. C, Histograms comparing the mean values (SEM) of the number of BrdU+ cells that incorporated BrdU at day 3 and survived 27 d later in the different deafferented VN. *p < 0.0001 compared with the same VN in other groups.

Next, to assess the phenotype of the proliferative cells, BrdU was administered 3 d after UVN and the phenotype of the BrdU+ cells was analyzed 27 d later (Groups 3, 5, and 7, as described in the “Study design” section). Results showed that a substantial number of cells that had incorporated BrdU at day 3 survived up to 30 d in the NaCl-infused group. We also observed that BDNF strongly increased the number of cells that had survived at this time point (Fig. 2C), whereas K252a blocked this effect, decreasing the number of BrdU+ surviving cells by ∼2-fold compared with UVN-NaCl group. Therefore, our results suggest that, after UVN, BDNF treatment facilitates, not only cell proliferation, but also cell survival in the deafferented VN.

BDNF treatment for 30 d increases astrocytes and microglia on the deafferented side

Next, we examined the impact of BDNF on astrocytes and microglia in the deafferented VN. Results showed that BDNF infused continuously for 30 d significantly increased the average number of GFAP cells in all the VN compared with the sham and the UVN-NaCl groups (average of all VN, sham: 20,876.73 cells, UVN-NaCl: 94,559 cells, UVN-BDNF: 163,759.09 cells), whereas administration of K252a significantly decreased this population in a proportion almost similar to what is observed in sham cats (UVN-K252a: 39,473.3 cells, p < 0.0001; Fig. 3A,B).

Figure 3.
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Figure 3.

BDNF treatment increases astrocytic and microglial cell population in the VN. A, C, Illustrations of GFAP− (A) or IBA1+ (C) cells in the left (lesioned) MVN of sham, UVN-NaCl, UVN-BDNF, and UVN-K252a animals 30 d after UVN. Scale bars: A, 50 μm; B, 20 μm. n = 4 animals per group. B, D, Histograms showing the effects of NaCl, BDNF, or K252a continuous infusion on the number of GFAP− (B) or IBA1+ (D) cells in the four VN 30 d after UVN. Only values recorded on the lesioned side are illustrated and data from both sides of the sham group were pooled for direct comparison with the subgroups of vestibular deafferented cats. SEMs are shown as vertical lines. Analyzes were assessed by ANOVA followed by Scheffe test for all of the VN and all groups (*p < 0.0001 vs the same VN of other groups).

We also observed that UVN induced a significant increase in microglial reaction at the day 30 after lesion in all of the deafferented VN of animals infused with NaCl. This effect was enhanced by BDNF, whereas K252a blocked this effect because we observed a number of IBA1+ cells almost similar to basal sham conditions (average of all VN, sham: 6880.05 cells, UVN-NaCl: 32,017.93 cells, UVN-BDNF: 57,732.74 cells, UVN-K252: 11,877.71 cells, p < 0.0001; Fig. 3C,D). Together, these results demonstrate that BDNF is a key modulator of post-UVN local increases in astrocytes and microglia.

BDNF treatment for 30 d increases the number of cholinergic and GABAergic neurons

To determine whether BDNF influences neuromodulator systems involved in vestibular compensation processes, we used ChAT and GAD67 immunomarkers to label cholinergic and GABAergic neurons in the VN 30 d after UVN. The results indicated that, in the sham group, the number of ChAT+ neurons was moderate and symmetric in both sides of the MVN (60.0 cells). Such proportion was comparable to the intact side of the MVN in all experimental groups (intact side, UVN-NaCl: 65 cells, UVN-BDNF: 68.7 cells, UVN-K252a: 58.0 cells). However, on the deafferented side, the number of ChAT+ neurons was significantly increased in the UVN-NaCl group and was increased by BDNF (UVN-NaCl: 104.5 cells, UVN-BDNF: 176.8 cells, p < 0.0001), whereas K252a did not have any effect (UVN-K252a: 56.8 cells). This suggests that UVN upregulates cholinergic neurons exclusively on the lesioned side and this effect is reinforced by BDNF but blocked by K252a administration (Fig. 4B,C).

Figure 4.
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Figure 4.

One month after UVN, BDNF treatment increases the number of GABAergic and cholinergic neurons in the VN. A, Illustrations of GAD67 immunoreactivity in the in the right (intact) and left (lesioned) MVN of sham, UVN-NaCl, UVN-BDNF, and UVN-K252a animals 30 d after UVN. Scale bar, 40 μm. n = 4 animals per group. B, Illustrations of ChAT immunoreactivity in the left (lesioned) MVN of sham, UVN-NaCl, UVN-BDNF, and UVN-K252a animals 30 d after UVN. Scale bar, 20 μm. n = 4 animals per group. C, Histograms showing the effects of NaCl, BDNF, or K252a continuous infusion on the number of ChAT+ neurons in both right (intact) and left (lesioned) medial VN 30 d after UVN. D, Histograms showing the effects of NaCl, BDNF, or K252a continuous infusion on the number of GAD67+ neurons in both right (intact) and left (lesioned) medial VN 30 d after UVN. SEMs are shown as vertical lines. Different letters indicate significant differences between groups of animals: a, significantly different from sham non-lesioned group; b, significantly different from UVN-NaCl group; c, significantly different from intact MVN of UVN-K252a group. Analyses were assessed by ANOVA followed by Scheffe test for all of the VN and all groups (p < 0.0001).

Although the number of GAD67+ neurons was moderate and symmetric in both sides of the MVN in the sham group (95.9 cells), UVN increased the number of GAD67+ exclusively on the deafferented side (365.1 cells, p < 0.0001 vs both the sham group and the intact side). In contrast, a significant and symmetrical bilateral increase (p < 0.0001) was observed in the UVN-BDNF group (intact side: 368.2 cells, deafferented side: 372.0 cells) and a smaller but significant increase of GAD67+ cells was also detected in the deafferented MVN of the UVN-K252a group compared with the sham group (186.4 cells, p < 0.0001; Fig. 2A,D). Overall, this finding suggests that, in vestibular pathological conditions, BDNF modulates cholinergic and GABAergic neuromodulation in the VN complexes.

BDNF influences the cell differentiation pattern of newborn cells in the deafferented VN

The new cells that survived more than several weeks after UVN are supposed to differentiate and to acquire a neurochemical phenotype ∼20 d after birth. Cell differentiation was investigated by double immunohistochemical labeling using BrdU combined with one of the four cell-type-specific markers: GFAP, NeuN, IBA1, or GAD67. To avoid the possibility that cells being BrdU+/IBA1+ reflects phagocytosis of dying proliferating cells by microglia, we counted only IBA1+ cells that displayed the typical ramified morphology and in which BrdU staining was in the center of the cell. As already described (Tighilet et al., 2007), no colocalization of BrdU+/ChAT+ was found in any group (data not shown), suggesting that newly generated cells in the deafferented VN did not differentiate into cholinergic neurons.

The photomicrographs in Figure 5, A and B, show the colocalization of BrdU with GFAP, NeuN, IBA1, and GAD67 cells observed in the deafferented MVN at day 30 after lesion in the UVN-BDNF (Fig. 5A) and UVN-K252a (Fig. 5B) groups. As described previously (Dutheil et al., 2013), in the UVN-NaCl group, the newly generated cells differentiated approximately in similar proportion in all the VN (Fig. 5C). Many of the BrdU+ cells were double-immunolabeled with NeuN in all experimental groups, indicating that some new cells differentiated into new non-GABAergic neurons and acquired a different neuronal phenotype (Fig. 5C–E). As for the UVN NaCl-group, when BDNF was infused for 30 d, the newly generated cells differentiated approximately in similar proportion in the MVN (12% GFAP, 14% IBA1, and 11% NeuN, respectively), IVN (12%, 13%, and 12%, respectively), and LVN (10%, 10%, and 8%, respectively) (Fig. 5A,D). In contrast, in the SVN, GFAP and IBA1 labeling were higher (12% and 14%, respectively) than NeuN labeling (5%). Interestingly, a larger soma surface with an enhanced dendritic branching of BrdU+/GAD67+ cells was observed in the group treated with BDNF (Fig. 5A) compared with those treated with K252a, where BrdU+/GAD67+ cells exhibited both smaller soma and dendritic fields (Fig. 5B). As for the rate of cell proliferation and survival, the number of BrdU+ cells that differentiated into astrocytes (BrdU+/GFAP+ cells), neurons (BrdU+/NeuN+ cells), microglial (BrdU+/IBA1+ cells), and GABAergic neurons (BrdU+/GAD67+) was four times greater in the UVN-BDNF group and two times lower in the UVN-K252a group compared with the UVN-NaCl group. Overall, these findings show that, in all the experimental groups, the newly generated cells differentiated in the four different lineages approximately in similar proportion in all VN.

Figure 5.
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Figure 5.

BDNF influences the cell differentiation pattern of newborn cells in the deafferented VN. Confocal analysis of differentiated newly generated cells by double immunostainings processed on consecutive serial sections in the deafferented MVN of cats infused with BDNF (A) or K252a (B) infusion from days 0–30 after UVN. The BrdU+ nuclei are shown in red and the other markers of differentiation (GFAP, IBA1, NeuN, and GAD67) are shown in green. Histograms illustrating the percentage are defined as the ratio between the mean number of immunopostive elements colocalizing a cell-type marker (GFAP, IBA1, NeuN, or GAD 67) and BrdU relative to the total mean number of BrdU+ nuclei counted in the areas of quantification of the UVN-NaCl (C), UVN-BDNF (D), or UVN-K252a (E) groups. SEMs are shown as vertical lines.

KCC2 and GABAAR expression are modulated by the vestibular lesion and by BDNF infusion

To determine how BDNF can accelerate vestibular functional recovery, we studied some of the local microenvironment changes that could influence network excitability in the first 3 d after UVN. We focused our analysis on the giant neurons of the LVN because it contains excitatory glutamatergic neuronsinvolved in vestibulo-spinal pathways regulating posturo-locomotor functions. Two specific markers of neuronal excitability were selected: the cation-chloride cotransporters KCC2, which determines the hyperpolarizing action of GABA, and GABAARs. In the UVN-NaCl group, we observed a significant decrease of KCC2 expression in the deafferented (ipsilateral) side (−32% vs sham group, p < 0.05; Fig. 6A,B), whereas a significant increase was found in the intact (contralateral) side (+28% vs sham group, p < 0.05), revealing a significant asymmetry between the two sides of the VN complex (p < 0.001) in this short time window. In addition, GABAAR expression was significantly increased in both the lesioned side (+88%, p < 0.0001) and the intact side (+186%, p < 0.0001) of the UVN-NaCl animals.

Figure 6.
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Figure 6.

KCC2 and GABAAR expression is modulated either by vestibular lesion and BDNF infusion. A, Confocal analysis of neurons immunostained with either the KCC2 or GABAAR located in the contralateral (intact) and ipsilateral (lesioned) lateral VN of sham or UVN animals infused with NaCl, BDNF, or K252a from days 0–3 after UVN. Scale bar, 20 μm. n = 4 animals per group. B, Histograms showing the effects of NaCl, BDNF, or K252a continuous infusion on KCC2 or GABAAR immunostainings in both intact and lesioned lateral VN 3 d after UVN. SEMs are shown as vertical lines. Data from both sides of sham group were pooled for direct comparison with the subgroups of vestibular deafferented cats. *Significant differences between groups of animals or between lesioned versus intact side in the same animal in the UVN-NaCl, UVN-BDNF, and UVN-K252a groups. *p < 0.005; **p < 0.001; ***p < 0.0001.

BDNF infusion did not alter KCC2 expression 3 d after UVN compared with the sham group, but did induce a bilateral downregulation of the GABAAR that was significant in the intact side compared with shams (−43%, p < 0.001; Fig. 6A,B). A significant decrease in GABAAR expression was also observed in the intact side compared with the deafferented side of UVN-BDNF animals (−23%, p < 0.005). Therefore, BDNF seems to be involved in the regulation of GABAAR expression in an early time window after UVN (days 1–3). Interestingly, blocking the action of endogenous BDNF by K252a infusion during these first 3 d after UVN led to a significant bilateral increase of KCC2 and GABAAR expression in the deafferented side (+70%, p < 0.05 for KCC2 and +100%, p < 0.001 for GABAAR) and the intact side (+128%, p < 0.001 for KCC2 and +183%, p < 0.0001 for GABAAR) compared with shams. Overall, this suggests that blocking BDNF action after UVN increases both KCC2 and GABAAR immunolabeling.

Discussion

We report here for the first time that BDNF potentiates vestibular neurogenesis and accelerates functional recovery after vestibular injury. We also show that KCC2 and GABAARs undergo remarkable fluctuations within the VN, suggesting that GABA acquires a transient depolarizing action in the VN during the recovery period. This novel plasticity mechanism could explain in part how the VN returns to electrophysiological homeostasis, considered in the literature to be a key parameter of vestibular compensation.

Neurotrophins and their TrK receptors are widely distributed in the main VN in mammals (Furukawa et al., 1998; Zhang et al., 2003, 2007; Lacour and Tighilet, 2010). Here, we show that BDNF treatment accelerates the restoration of oculomotor and posturo-locomotor functions. Consistent with these experiments, a previous study of intra-VN administration of BDNF in the guinea pig showed decreased spontaneous nystagmus after vestibular damage (Maingay et al., 2000). Moreover, we found that TrkB blockade delays the recovery of all impaired functions, especially posturo-locomotor activity, as described in another study conducted in guinea pigs showing that BDNF blockade delayed postural compensation after unilateral labyrinthectomy (Bolger et al., 1999). Therefore, vestibular compensation time course is strongly accelerated by local action of BDNF in the VN.

We have shown that BDNF and its receptor TrkB are strongly upregulated at 3 d after UVN in the deafferented VN, which coincides with the highest production rate of newborn cells (Lacour and Tighilet, 2010). Here, we show that continuous BDNF administration into the fourth ventricle after UVN significantly increases the production of newly generated cells, confirming the proneurogenic properties of this growth factor in vivo. Similarly, a previous study showed that BDNF infusion into the lateral ventricle of the adult rat leads to new neurons in the striatum, septum, thalamus, and hypothalamus (Pencea et al., 2001). Next, we used K252a and showed that it strongly attenuated the proneurogenic action of UVN. One month after the lesion, we observed that BDNF increased cell survival compared with saline, whereas K252a-treated animals had the lowest rate of cell survival and did not achieve complete vestibular compensation. More specifically, we observed that newborn GABAergic neurons exhibited both smaller soma and dendritic fields in K252a-treated animals, whereas these cells exhibited a larger soma surface with enhanced dendritic branching in the UVN-BDNF group. Axon growth and dendrite development are key processes for the establishment of a functional neuronal network and it was reported recently that dendritic branching is BDNF dependent (Ribeiro et al., 2015). Together, our results demonstrate that BDNF action through TrkB receptors amplifies cell proliferation, survival, and development of functional dendritic branching in the deafferented VN of the vestibular-lesioned cats. We suggest that BDNF may contribute to the integration of newborn neurons into functional networks, which would favor the behavioral recovery.

Astrocyte-expressed factors are known to modulate neural progenitor cell differentiation (Song et al., 2002; Gonzalez-Perez and Quiñones-Hinojosa, 2012) and are potent regulators of synapse formation, tuning, and maintenance in both developing and adult brain (Xu et al., 2009; Roberts et al., 2010; Chung et al., 2013). Microglia are also involved in synapse elimination and can release various neurotrophic and anti-inflammatory factors favoring cell proliferation, survival, and regeneration (Aguzzi et al., 2013; Cherry et al., 2014). M2-like microglial activation has been shown to be protective and to belong to the healthy neurogenic niche in vitro by maintaining normal neuronal precursor cell activity (Butovsky et al., 2006; Walton et al., 2006). In addition, environmental enrichment known to increase neurogenesis is accompanied by an increase in anti-inflammatory-type microglia (Ziv et al., 2006). Likewise, in this study, we observed an increase in both astrocytes and microglia concurrently with the formation of new neurons at day 3 after UVN that were all promoted by BDNF and reduced by K252a. We hypothesize that astrocytes and microglia promote a benefic environment for neurogenesis in our model and could also remodel the synaptic environment by removing unwanted and dysfunctional synapses uncorrelated to activity. Such a mechanism would shape neuronal networks and help to keep the most efficient connections to rebalance electrical activity between the deafferented and intact VN.

We have shown that the expression of cholinergic neurons increases after UVN, but newly generated neurons do not acquire a cholinergic phenotype (Tighilet and Lacour, 1998; Tighilet et al., 2007). This observation suggests that, after deafferentation, ChAT protein expression is upregulated locally. In the present study, we confirmed this result and also show that, 30 d after lesion, BDNF infusion potentiated this effect. Likewise, it has been described that BDNF enhances acetylcholine release (Auld et al., 2001) and promotes a cholinergic phenotype in basal forebrain neurons (Nonner et al., 2000) through a TrkB-dependent mechanism (Burgess and Aubert, 2006). Interestingly, ACh agonists and acetylcholinesterase inhibitors have also been found to increase the firing activity of VN neurons in vitro and in vivo through muscarinic and nicotinic receptors (Yamamoto, 1967; Ujihara et al., 1989; Phelan and Gallagher, 1992). Therefore, we strongly suggest that BDNF-TrkB signaling causes the upregulation of ChAT expression in the ipsilateral VN of the BDNF-UVN group and may help rebalance the activity between the ipsilateral and contralateral VN, which is considered to be a pivotal step in behavioral recovery.

GABAergic transmission is essential for healthy brain physiology during development and throughout adult life; any discrepancy between excitation and inhibition can cause severe pathological conditions such as epilepsy (Ben-Ari, 2014). Recent evidence indicates that GABAergic signaling alters post-UVN-related plasticity mechanisms occurring in the VN and can influence directly the time course of vestibular compensation (Dutheil et al., 2013). Consistent with our previous studies (Tighilet and Lacour, 2001; Dutheil et al., 2009, 2011, 2013), we confirm here that UVN promotes proliferation of GABAergic neurons exclusively on the ipsilateral side, which likely helps to rebalance the ratio of excitation to inhibition between the two VN. Surprisingly, we observed that BDNF infusion led to a symmetric increase of GABAergic neurons in both the intact and lesioned VN, whereas a TrkB receptor antagonist blocked this effect. TrkB and its ligand, BDNF, could modulate GABAergic transmission in the VN, as demonstrated in the rat hippocampus (Frerking et al., 1998) and mouse cerebellum (Rico et al., 2002). These data imply that BDNF and GABA both facilitate a return to electrophysiological homeostasis between the VN on the deafferented and intact sides, which is a key component of vestibular functional recovery.

Restoration of vestibular neurons' excitability on the deafferented side is known to be a key parameter for functional recovery (Lacour and Tighilet, 2010). It is also well accepted that the excitability level of the local environment significantly affects adult neurogenesis (Chun et al., 2006; Segi-Nishida et al., 2008; Kokaia, 2011). We thus examined how the TrKB-BDNF signaling pathway can accelerate vestibular compensation, focusing on two neuronal markers that reflect the level of excitability: the cation-chloride cotransporter KCC2 and the GABAAR. Downregulation of KCC2 has been shown to decrease the chloride extrusion capacity of the cell, which leads to intracellular accumulation of [Cl−] ions and thus induces a depolarizing outward chloride current from GABAARs in response to GABA (Coull et al., 2003, 2005), as illustrated in Figure 7. Here, we show that whereas GABAAR expression is increased on both sides of the LVN 3 d after UVN in lesioned cats infused with saline, KCC2 expression is asymmetrical (i.e., decreased in the lesioned LVN and increased in the intact LVN). Consistent with this result, several studies have shown a decreased KCC2 expression after a different type of lesion in the adult mammal brain (Cramer et al., 2008; Mòdol et al., 2014a, 2014b), thus triggering a reduction in KCC2 expression responsible for an increase in local neurons' excitability (i.e., a depolarizing response to GABA; Nabekura et al., 2002; Toyoda et al., 2003; Kahle et al., 2008; Boulenguez et al., 2010; Sadlaoud et al., 2010).

Figure 7.
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Figure 7.

Summary of the cascade of events underlying modulation of chloride homeostasis in the deafferented VN after UVN. In the adult, in physiological conditions or on the intact side of the VN (right side), the presence of KCC2 on the cells leads to a low [Cl−]i concentration. When GABA activates GABAAR, it then triggers a hyperpolarizing action through the influx of Cl−. After UVN (left side), microglia and astrocytes are strongly upregulated in the deafferented VN. We hypothesize that microglia, as well as nerve terminals, release BDNF, which in turn activates TrkB receptors and downregulates KCC2 expression. A lower concentration in KCC2 then decreases the chloride extrusion capacity of the cell; [Cl−]i then accumulates and causes a collapse in the transmembrane chloride gradient. The anion flux being inverted, GABAAR activation leads, in such conditions, to an efflux of Cl−, thus increasing the excitability of the cells. Interestingly, at the same time point (3 d after UVN), a peak of BrdU+ cells is observed on the lesioned side of the VN and is correlated with a peak of BDNF expression as well. This suggests that BDNF, released by both neurons and glia, could modulate cell proliferation, survival, and excitability.

In addition, the microglia–BDNF–TrkB signaling also appears to be able to shift neurons toward a more excitable state. Under nerve injury conditions, microglia become activated (McMahon et al., 2005) and express purinergic P2X4 receptors (Tsuda et al., 2003). Stimulation of P2X4 receptors cause microglia to secrete BDNF, which in turn acts on TrkB receptors' neurons that downregulate KCC2 (Rivera et al., 2002, 2004; Ferrini and De Koninck, 2013), thus decreasing the chloride extrusion capacity of the cell. A similar mechanism could be envisioned in our UVN model, in which microglia are significantly upregulated in the deafferented VN. BDNF would act on TrkB receptors and cause an excitatory action of GABA through the observed downregulation of KCC2 expression. Interestingly, it has been shown that blockade of microglia–BDNF–TrkB signaling at the spinal level caused a return of EGABA to more negative values in spinal cord slices from rats with established allodynia (Coull et al., 2005). Consistent with this, our results showed a downregulation of microglia in the VN of the UVN cats in which BDNF action was blocked, combined with an upregulation of both KCC2 and GABAAR expression. Behaviorally, this group showed a very slow and incomplete functional recovery. We therefore suggest that blocking BDNF action and its cellular consequences on KCC2 and GABAAR expression causes a strong inhibitory action of GABA in the vestibular environment of these animals, which is responsible for their slower and incomplete compensation. In contrast, because we found that, 3 d after UVN, BDNF infusion downregulated GABAAR and did not induce KCC2 expression changes in the LVN, we believe that bilateral reduction of GABAAR immunoreactivity is likely to reflect a reduction of local inhibition that seems beneficial for vestibular compensation. Collectively, the data described here reveal that KCC2 modulation appears to be a novel and important mechanism contributing to vestibular compensation through the restoration of spontaneous activity in the deafferented VN. Our results also highlight a short therapeutic critical time window between 1 and 3 d after UVN when some markers of excitability are locally altered and during which the effects of pharmacological treatments are likely to be more efficient.

In conclusion, this study provides new information about the remarkable plastic changes in the structural organization and functional properties of neuronal circuits during vestibular compensation. It highlights the importance of both BDNF and KCC2 in the normalization of local excitability through GABAARs, which could represent a novel treatment strategy.

Footnotes

  • This work was supported by the Ministère de l'enseignement supérieur et de la recherche and the Centre National de la Recherche Scientifique (Unité Mixte de Recherche 7260 Aix-Marseille Université). We thank Dr. Alexandra Thomas from Yale University for reviewing and improving this manuscript, Valérie Gilbert and Elodie Mansour for taking care of the animals, and Abdessadek El Ahmadi for expertise in statistical analysis.

  • The authors declare no competing financial interests.

  • S.D.'s temporary affiliation was Laboratory of Molecular Psychiatry, Department of Psychiatry, Yale University School of Medicine, 34 Park Street, New Haven, CT 06508, USA.

  • Correspondence should be addressed to Brahim Tighilet, Laboratoire de Neurosciences Intégratives et Adaptatives, UMR 7260 Aix-Marseille Université-CNRS, Fédération de Recherche 3C, 3 Place Victor Hugo, 13331 Marseille Cedex 03, France. brahim.tighilet{at}univ-amu.fr

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BDNF Signaling Promotes Vestibular Compensation by Increasing Neurogenesis and Remodeling the Expression of Potassium-Chloride Cotransporter KCC2 and GABAA Receptor in the Vestibular Nuclei
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BDNF Signaling Promotes Vestibular Compensation by Increasing Neurogenesis and Remodeling the Expression of Potassium-Chloride Cotransporter KCC2 and GABAA Receptor in the Vestibular Nuclei
Sophie Dutheil, Isabelle Watabe, Karina Sadlaoud, Alain Tonetto, Brahim Tighilet
Journal of Neuroscience 8 June 2016, 36 (23) 6199-6212; DOI: 10.1523/JNEUROSCI.0945-16.2016

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BDNF Signaling Promotes Vestibular Compensation by Increasing Neurogenesis and Remodeling the Expression of Potassium-Chloride Cotransporter KCC2 and GABAA Receptor in the Vestibular Nuclei
Sophie Dutheil, Isabelle Watabe, Karina Sadlaoud, Alain Tonetto, Brahim Tighilet
Journal of Neuroscience 8 June 2016, 36 (23) 6199-6212; DOI: 10.1523/JNEUROSCI.0945-16.2016
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Keywords

  • BDNF
  • GABA
  • gliogenesis
  • KCC2
  • neurogenesis
  • vestibular compensation

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