Abstract
Cholinergic vulnerability, characterized by loss of acetylcholine (ACh), is one of the hallmarks of Alzheimer's disease (AD). Previous work has suggested that decreased ACh activity in AD may contribute to pathological changes through global alterations in alternative splicing. This occurs, at least partially, via the regulation of the expression of a critical protein family in RNA processing, heterogeneous nuclear ribonucleoprotein (hnRNP) A/B proteins. These proteins regulate several steps of RNA metabolism, including alternative splicing, RNA trafficking, miRNA export, and gene expression, providing multilevel surveillance in RNA functions. To investigate the mechanism by which cholinergic tone regulates hnRNPA2/B1 expression, we used a combination of genetic mouse models and in vivo and in vitro techniques. Decreasing cholinergic tone reduced levels of hnRNPA2/B1, whereas increasing cholinergic signaling in vivo increased expression of hnRNPA2/B1. This effect was not due to decreased hnRNPA2/B1 mRNA expression, increased aggregation, or degradation of the protein, but rather to decreased mRNA translation by nonsense-mediated decay regulation of translation. Cell culture and knock-out mice experiments demonstrated that M1 muscarinic signaling is critical for cholinergic control of hnRNPA2/B1 protein levels. Our experiments suggest an intricate regulation of hnRNPA2/B1 levels by cholinergic activity that interferes with alternative splicing in targeted neurons mimicking deficits found in AD.
SIGNIFICANCE STATEMENT In Alzheimer's disease, degeneration of basal forebrain cholinergic neurons is an early event. These neurons communicate with target cells and regulate their long-term activity by poorly understood mechanisms. Recently, the splicing factor hnRNPA2/B, which is decreased in Alzheimer's disease, was implicated as a potential mediator of long-term cholinergic regulation. Here, we demonstrate a mechanism by which cholinergic signaling controls the translation of hnRNPA2/B1 mRNA by activation of M1 muscarinic type receptors. Loss of cholinergic activity can have profound effects in target cells by modulating hnRNPA2/B1 levels.
Introduction
Dementia affects roughly 44 million individuals worldwide and represents a large economic burden (Wimo et al., 2013). Individuals affected with Alzheimer's disease (AD) present a profound decrease in basal forebrain cholinergic neurons (Whitehouse et al., 1982). These findings led to the cholinergic hypothesis of AD and the use of cholinesterase inhibitors to mitigate cholinergic failure (Bartus et al., 1982). Previous work suggests that the use of cholinesterase inhibitors for one year in possible prodromal AD-affected individuals halved rates of hippocampal atrophy, suggesting an intricate relationship between cholinergic tone and neurodegeneration (Dubois et al., 2015).
Cholinergic tone can modulate signal processing by changing electrical properties of cells and by modulating intracellular signaling (Dajas-Bailador and Wonnacott, 2004; Soreq, 2015). In addition, it has become clear that cholinergic signaling can also regulate long-term gene expression, miRNAs (Shaked et al., 2009; Soreq, 2015), and alternative splicing (Berson et al., 2012; Kolisnyk et al., 2013a), all of which can modulate functional properties of cells (Blencowe, 2006; Novarino et al., 2014). A key protein that regulates splicing events in AD is the heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1, which is reduced in brains of AD patients due to cholinergic deficiency (Berson et al., 2012; Kolisnyk et al., 2013a) and is reduced in mice with a conditional deletion of the vesicular acetylcholine (ACh) transporter in the forebrain (VAChT) (Kolisnyk et al., 2013a).
hnRNPs are a large family of proteins that package pre-mRNA into larger hnRNP particles (Dreyfuss et al., 1993). Each of the hnRNPs contain distinct RNA binding motifs, which allows them to exert their roles in pre-mRNA processing (Weighardt et al., 1996; Black, 2003). hnRNPA2/B1 is one of the major hnRNP isoforms in the brain regulating alternative splicing and the transport of mRNA to distal cellular processes (Han et al., 2010). Importantly, knockdown of hnRNPA2/B1 in vivo caused impairments in learning and memory (Berson et al., 2012). Together, these findings suggest a critical role for this RNA binding protein in neuronal function and cognition.
Previous work suggests that cholinergic tone can regulate levels of hnRNPA2/B1; however, the mechanisms involved are unclear. Here, we show that hnRNPA2/B1 is a cholinergic controlled splicing factor. M1 muscarinic receptor activity is critical for cholinergic regulation of hnRNPA2B1 translation. This work provides a new mechanism by which acetylcholine can influence targeted neurons, leading to potential widespread changes in neuronal function.
Materials and Methods
Mouse lines.
All animals with targeted elimination of VAChT were generated using the VAChTflox/flox mouse described by Martins-Silva et al. (2011). To eliminate VAChT from the forebrain, VAChTflox/flox mice were crossed with C57BL/6J-Tg(Nkx2–1-cre)2Sand/J mice (Xu et al., 2008) to generate VAChTNkx2.1-Cre-flox/flox (Al-Onaizi et al., 2016). To eliminate VAChT from the striatum, the VAChTflox/flox mice were crossed with D2-Cre mice (Drd2, line ER44) to generate VAChTD2-Cre-flox/flox (Guzman et al., 2011). VAChT-overexpressing ChAT-ChR2-EYFP mice were from The Jackson Laboratory [B6.Cg-Tg(Chat-Cop4*H134R/EYFP)6Gfng/J; Zhao et al., 2011]. Generation of TgR (Shaked et al., 2009), M1 KO (Hamilton et al., 1997), and M4 KO mice (Gomeza et al., 1999) was described previously. All procedures were conducted in accordance with guidelines of the Canadian Council of Animal Care at the University of Western Ontario with an approved institutional animal protocol (2008-127). Only male mice were used for all experiments.
Immunofluorescence.
Immunofluorescence experiments were performed as described previously (Guzman et al., 2011). Primary antibodies used were anti-hnRNPA2/B1 (1:200; Santa Cruz Biotechnology, catalog #sc-10035), anti-NeuN (1:200; PhosphoSolutions, catalog #583-FOX3), and anti-GFAP (1:500; Abcam, catalog #ab7260). Sections were visualized with a Zeiss LSM 510 Meta confocal system (10×, 40×, and 63× objectives), and a 488 nm Ar laser and 633 nm HeNe laser were used for excitation of fluorophores.
Western blotting.
Mouse hippocampi were collected, proteins were isolated, and immunoblotting was performed as described previously using RIPA lysis buffer supplemented with protease and phosphatase inhibitors (Guzman et al., 2011). Band intensities were quantified using FluoroChemQ software (Thermo Fisher Scientific).
Subcellular fractionation.
To isolate nuclear and cytoplasmic proteins from hippocampal tissue, the NE-PER Nuclear Protein Extraction Kit (Thermo Fisher Scientific) was used following the manufacturer's instructions.
Sarkosyl insolubility.
Isolation of sarkosyl-insoluble protein was performed as described previously (Bai et al., 2013). Hippocampal tissue was homogenized in a low salt buffer [10 mm Tris, pH 7.5, 5 mm EDTA, 1 mm DTT, 10% (w/v) sucrose, Sigma protease inhibitor cocktail; ∼10 ml buffer per gram tissue] to detect total protein, a detergent buffer [the low salt buffer with the addition of 1% (w/v) sarkosyl (N-lauroylsarcosine)] for the sarkosyl-soluble fraction, and finally 8 m urea with 2% (w/v) SDS for the sarkosyl-insoluble fraction.
Ubiquitination assay.
The ubiquitination status of the hnRNPA2/B1 protein was determined using previously described protocols (Choo and Zhang, 2009). Briefly, the hnRNPA2/B1 protein was immunoprecipitated using the Santa Cruz Biotechnology (catalog #sc-10035) antibody and run on an SDS-PAGE gel. The gel was then blotted with an anti-ubiquitin antibody (Abcam, catalog #ab7780).
Stereotaxic injections of adeno-associated virus.
Injection of AAV virus was described previously (Al-Onaizi et al., 2016). Briefly, mice were anesthetized with ketamine (100 mg/kg) and xylazine (25 mg/kg), and 1 μl (titer of ∼1013 Gene Copies/ml) of AAV8-GFP-Cre or control virus (AAV8-GFP, Vector BioLabs) was injected into the medial septum (0.98 anteroposterior, 0.1 laterolateral, and 4.1 dorsoventral) of VAChTflox/flox mice. A recovery period of 4 weeks was given to allow transgene expression before subsequent analyses.
RNA sequencing.
Total RNA was extracted from hippocampal tissues. The cDNA library was prepared using a TruSeq Stranded Total Sample Preparation kit (Illumina) and run in a HiSeq 2500 platform. Data sets are available on ArrayExpress (http://www.ebi.ac.uk/arrayexpress/) under accession number E-MTAB-3897.
qPCR and RT-PCR.
To measure mRNA expression, total RNA was extracted from freshly dissected hippocampal tissue using the Aurum Total RNA for Fatty and Fibrous Tissue kit (Bio-Rad) according to the manufacturer's instructions. cDNA synthesis and qPCR analysis were performed as described previously (Guzman et al., 2011). For alternative splicing experiments, the alternative exon levels were normalized to a constitutively expressed exon from the same gene. RT-PCR was performed as described previously (Ribeiro et al., 2007).
Isolation of polysomal RNA.
Isolation of polysomal RNA was performed as described previously (Wagnon et al., 2012). Samples were homogenized in 1.5 ml of ice-cold lysis buffer [20 mm Tris-HCl, pH 7.4, 3 mm MgCl2, 10 mm NaCl, 2% sucrose, 0.3% Triton X-100, 2 mm vanadyl ribonucleoside complexes (VRCs)] supplemented with protease inhibitors (cOmplete, mini, EDTA-free, Roche). The homogenate was then centrifuged at 10,000 × g for 10 min at 4°C, and the supernatant was transferred to a fresh tube. The lysate was treated with either 30 mm EDTA or 0.1 mg/ml RNase A for 30 min on ice; if it was to be treated with EDTA, VRCs were not included in the lysis buffer. Lysate was then carefully layered onto 15–55% of sucrose in 25 mm Tris-HCl, pH 7.4, 25 mm NaCl, 5 mm MgCl2 (and 30 mm EDTA for the EDTA samples). Gradients were ultracentrifuged in a Beckman Instruments SW40Ti rotor at 150,000 × g for 2 h and 25 min at 4°C.
To isolate RNA from the fractions, RNA was collected into 1.5 ml of 7.7 m guanidine-HCl, 2 ml of 100% ethanol was added, and samples were stored at −20°C. Samples were then centrifuged at 4000 × g for 50 min at 4°C. The supernatant was removed, and 200 μl DEPC-treated H2O was added to the pellet. To precipitate the RNA, 500 μl of 100% ethanol, 20 μl of 3 m sodium acetate, pH 5.2, and 10 μg glycogen were added, and the samples were stored overnight at −20°C. The samples were then centrifuged at 14,000 × g for 30 min at 4°C. Pellets were washed with ice-cold 75% ethanol and air dried for 15 min at room temperature. RNA was resuspended in 30 μl DEPC-treated H2O and quantified by nanodrop before being converted into cDNA using the Applied Biosystems cDNA conversion kit. RT-PCR was then performed using primers designed to amplify hnRNPA2/B1 or β-Actin.
Primary neuronal cultures.
Primary cultures of hippocampal neurons from embryonic day 17 mouse embryos were obtained as described previously (Beraldo et al., 2013). Cultures were maintained in Neurobasal medium with 2% B-27 supplement (Invitrogen). On day 4, cytosine arabinoside (2 μm; Sigma) was added to prevent astrocyte growth. Half of the culture medium was changed every 2 or 3 d. Neurons were cultured for 15 d.
Pharmacological manipulations in primary neuronal cultures.
On the 15th day of culture, neurons were treated with different doses of carbachol (0, 5, 10, or 50 μm) dissolved in saline, and 48 h later, total protein was isolated and levels of hnRNPA2/B1 were determined by Western blotting and immunofluorescence. To evaluate the contribution of nicotinic and muscarinic receptors in regulating hnRNPA2/B1 protein levels, neurons were pretreated with mechamylamine (100 μm), atropine (100 μm), or both. Then, 1 h later, neurons were treated with carbachol (10 μm). Finally, to assess the effect of M1 muscarinic activation, neurons were treated with different doses of AF102B (0, 10, or 100 μm) dissolved in saline.
Statistical analysis.
Data are presented as mean ± SEM unless otherwise stated. GraphPad Prism 6 software was used for statistical analysis. Comparison between two experimental groups was done by Student's t test. When several experimental groups or treatments were analyzed, one-way ANOVA and, when appropriate, a Tukey's HSD post hoc comparison test were used.
Results
Cholinergic modulation of hnRNPA2/B1 protein levels
Previous experiments indicated that hnRNPA2/B1 is decreased in AD brains, but this is not modeled in genetic mouse models of AD (Berson et al., 2012). In contrast, either genetic or immunotoxin disruption of cholinergic tone led to decreased expression of hnRNPA2/B1 (Berson et al., 2012; Kolisnyk et al., 2013a). As expected, VAChTNkx2.1-Cre-flox/flox animals presented a robust decrease in hnRNPA2/B1 levels in the hippocampus (Fig. 1A).
The Nk2.1 promoter turns on Cre expression early during development (Xu et al., 2008), and, therefore, the resulting decrease in hnRNPA2/B1 levels could potentially be a result of developmental suppression of cholinergic tone, rather than being cholinergic regulated in adult mice. To test this possibility, we deleted the VAChT gene specifically in medial septum neurons and parts of the diagonal band (Al-Onaizi et al., 2016), which provides most of the hippocampal cholinergic innervation, of adult VAChTflox/flox mice using AAV8-Cre virus. AAV8-Cre-injected mice showed interrelated decline of both VAChT and hnRNPA2/B1 proteins, whereas AAV8-GFP-injected mice did not (Fig. 1B). There was a significant relationship between VAChT and hnRNPA2/B1 levels (r2 = 0.755, p < 0.001; Fig. 1B). In addition, we tested whether, in the striatum, elimination of cholinergic tone would affect hnRNPA2/B1 expression by using VAChTD2-Cre-flox/flox mice with selective striatal VAChT deficiency (Guzman et al., 2011). These mice showed no changes in hnRNPA2/B1 in their striatum (Fig. 1C), indicating hippocampal specificity of these effects.
If expression of hnRNPA2/B1 is a cholinergic-regulated process, one would expect that increased cholinergic tone should have opposite effects than those observed by decreased VAChT expression. ChAT-ChR2-EGFP mice overexpress VAChT in the hippocampus and consequently present increased cholinergic tone and ACh release (Kolisnyk et al., 2013b). These mice present increased hnRNPA2/B1 protein levels (Fig. 1D), suggesting that VAChT levels critically regulate hnRNPA2/B1 expression.
Decline of hnRNPA2/B1 could be solely related to VAChT depletion, or, alternatively, it could reflect the functional loss of ACh signaling in the CNS. To distinguish between these possibilities, we tested the hippocampus of TgR mice overexpressing soluble AChE (Shaked et al., 2009), which causes cholinergic insufficiency. TgR mice showed decrease in hnRNPA2/B1 levels similar to mice with decreased VAChT (Fig. 1E), suggesting that ACh synaptic levels are causally involved in the regulation of hnRNPA2/B1 expression. Cre expression by itself had no effect on hnRNPA2/B1 levels, as can be seen when we compared expression of hnRNPA2/B1 in the hippocampus of Nkx2.1-Cre and WT mice (Fig. 1F). Together, these data give strong support for the hypothesis that hnRNPA2/B1 is a cholinergic-regulated splicing factor.
RNA binding proteins such as hnRNPA2/B1 are predominantly expressed in the nucleus, but they can accumulate in the cytoplasm and cause neuronal toxicity (Wolozin, 2012; Kim et al., 2013). We therefore determined by immunofluorescence staining whether the localization of hnRNPA2/B1 is changed in response to decreased cholinergic tone. Compared to controls, VAChTNkx2.1-Cre-flox/flox had a decrease in hnRNPA2/B1 immunostaining in the CA1, CA3, and dentate gyrus regions of the hippocampus (Fig. 2A–D). hnRNPA2/B1 was present mainly in the nucleus of both neurons (labeled by NeuN; Fig. 2A–D) as well as in astrocytes (E) in control mice. We did not observe any shift in the localization of hnRNPA2/B1 in VAChT-deficient mice, only an overall decrease in the levels of staining. To confirm these observations, we used subcellular fractionation to assess whether VAChTNkx2.1-Cre-flox/flox mice show changes in hnRNPA2/B1 distribution between the nuclear and cytoplasmic fractions (Fig. 2F). In both control and VAChT-deficient mice, hnRNPA2/B1 was predominantly nuclear, following the distribution of the nuclear enzyme PIAS1 (Soares et al., 2013). However, VAChTNkx2.1-Cre-flox/flox mice showed consistently reduced nuclear hnRNPA2/B1 levels (Fig. 2F).
Mechanisms of cholinergic modulation of hnRNPA2/B1
To investigate mechanisms by which cholinergic tone may regulate the levels of hnRNPA2/B1 protein, we evaluated ubiquitination, a modification that can facilitate protein degradation by the proteasome (Hochstrasser, 1996). Immunoprecipitated hnRNPA2/B1 from the hippocampi of controls and VAChTNkx2.1-Cre-flox/flox mice was resolved by SDS-PAGE and probed with ubiquitin antibodies. VAChT-deficient mice showed no change in ubiquitination status of hnRNPA2/B1 protein when normalized to total hnRNPA2/B1 protein levels (Fig. 3A).
We also examined whether cholinergic tone affects aggregation of hnRNPA2/B1. Notably, hnRNPA2/B1 has a prion-like domain that favors increased aggregation when mutated (Kim et al., 2013). Protein aggregation was investigated by fractionating hippocampal extracts into sarkosyl-soluble and sarkosyl-insoluble fractions (Fig. 3B). In both controls and VAChT-deficient mice, hnRNPA2/B1 was mainly present in soluble fractions, unlike the U1-70k small nuclear ribonucleoprotein, which has been shown to be present in insoluble fractions (Bai et al., 2013; Fig. 3B). This result excluded the option that cholinergic tone increases aggregation of hnRNPA2/B1. Interestingly, despite different levels of hnRNPA2/B1 protein expression, both control and VAChT-deficient mice exhibited similar hnRNPA2/B1 mRNA levels as determined by RNA sequencing (Fig. 3C), as observed in AD brains (Berson et al., 2012).
It was reported previously that protein levels of hnRNPA2/B1 are directly proportional to changes in the RNA editing of the 3′ UTR of its mRNA, with a shift away from a nonsense-mediated decay (NMD)-sensitive transcript increasing protein levels (Bonomi et al., 2013; Fig. 3D). We evaluated by qPCR the ratio of NMD-sensitive to NMD-insensitive versions of the hnRNPA2/B1 transcript in the hippocampus of VAChTNkx2.1-Cre-flox/flox mice. Compared to controls, we observed a significant shift toward the NMD+ product in VAChT-deficient mice (Fig. 3E).
We then tested whether cholinergic tone modulation of NMD+ transcript could regulate hnRNPA2/B1 protein expression levels by limiting protein translation. For this, we studied the recruitment of hnRNPA2/B1 mRNA to ribosomes. Ribonucleotide-protein complexes (RNPs) were isolated from hippocampal lysates, and sucrose density gradient fractionation was used to separate polyribosomes from large neuronal RNA granules (Fig. 4A; Wagnon et al., 2012). Distribution of hnRNPA2/B1 transcripts in individual fractions was determined by RT-PCR in three individual mice of each genotype (Fig. 4B,C). In control mice, hnRNPA2/B1 mRNAs associated with polysomes and RNA granules, and treatment with EDTA, which dissociates mRNA from polysomes, equally distributed hnRNPA2/B1 mRNAs across fractions (Fig. 4D,E). However, in VAChTNkx2.1-Cre-flox/flox mice, distribution of hnRNPA2/B1 mRNAs was widespread throughout the fractions, resembling the distribution observed after EDTA treatment (Fig. 4D,E). The abundant β-actin mRNA remained unaltered between genotypes, demonstrating specificity toward hnRNPA2/B1 (Fig. 3F,G). These results indicate that decreased cholinergic tone leads to diminished translational capacity of hnRNPA2/B1 mRNA transcripts to modulate the efficiency of hnRNPA2/B1 protein translation.
Muscarinic signaling regulates hnRNPA2/B1 translation by an NMD mechanism
To further understand how cholinergic signaling regulates hnRNPA2/B1 levels, we treated neuronal hippocampal cultures from wild-type mice with the cholinergic mimetic carbachol (10 μm; Fig. 5A,B). This treatment effectively increased hnRNPA2/B1 protein levels in immunofluorescence and immunoblot experiments, and this effect could be blocked by muscarinic, but not by nicotinic antagonist treatment (Fig. 5C).
To study the contribution of muscarinic receptor subtypes, we evaluated hnRNPA2/B1 levels in the hippocampus of muscarinic receptor knock-out mice. Compared to wild-type mice, M1, but not M4, receptor knock-out mice showed a decrease in hnRNPA2/B1 protein levels resembling that in VAChTNkx2.1-Cre-flox/flox mice (Fig. 5D). Together, these experiments suggest that decreased cholinergic tone, likely due to insufficient M1 receptor activation, changes hnRNPA2/B1 protein levels by regulating mRNA translation.
To further investigate the importance of M1 muscarinic receptors in the regulation of hnRNPA2/B1 protein levels in hippocampal neurons, we treated neuronal hippocampal cultures from wild-type mice with the M1 muscarinic agonist AF102B (Fisher et al., 1989). Compared to saline-treated neurons, those treated with 100 μm of AF102B showed a significant increase in protein levels of hnRNPA2/B1 (Fig. 5E).
We then evaluated whether in vitro cholinergic regulation also changes the NMD+ product. Compared to saline treatment, carbachol shifted the expression of hnRNPA2/B1 RNA toward the NMD insensitive full-length mRNA product (Fig. 5F). Furthermore, cotreatment with atropine blocked this effect and returned the ratio to control levels, similar to what we observed for hnRNPA2/B1 protein levels (Fig. 5C). These data suggest that the ratio of NMD+ hnRNPA2/B1 gene products predicts change in protein levels, and that the regulation of NMD sensitivity is dependent on muscarinic signaling.
Discussion
In this study, we combined a variety of in vivo and in vitro techniques to evaluate the contribution of cholinergic signaling to expression levels of hippocampal hnRNPA2/B1 protein. Using a number of mouse lines, we demonstrated that hnRNPA2/B1 protein levels in the hippocampus are exquisitely sensitive to changes in cholinergic tone.
Interestingly, we observed no change in hnRNPA2/B1 protein levels in striatum- specific VAChT mutants, whereas there is a body of evidence that cholinergic signaling can affect the levels of this protein in both cortical and hippocampal regions in vivo (Berson et al., 2012; Kolisnyk et al., 2013a). Given these findings, it is likely that it is an intrinsic property of the target cells themselves that dictate their change in hnRNPA2/B1 translation in response to cholinergic activity. Our results highlight the critical role of the Gq coupled M1 muscarinic receptor in governing hnRNPA2/B1 protein levels.
Unlike the rare hnRNPA2/B1 mutation that increases aggregation and nuclear exclusion (Kim et al., 2013), we did not find aggregation of hnRNPA2/B1 in mice with forebrain cholinergic deficiency, suggesting that in these mice, and likely in AD brains, hnRNPA2/B1 dysfunction occurs by a separate and distinct mechanism. Furthermore, we did not see an increase in ubiquitination, suggesting that changes in hnRNPA2/B1 protein levels do not occur at the posttranslational level. In line with this, we found that it may in fact be abnormal translation that drives regulation of hnRNPA2/B1.
A common mechanism for the regulation of the translation of RNA binding proteins (RBPs) is regulation by unproductive splicing and translation (RUST), where the alternative spicing of a transcript affects its translation efficiency (Lareau et al., 2007; McGlincy and Smith, 2008). This may serve as a potential mechanism for cholinergic control of the translation of this RBP. Accordingly, we found that the levels of hnRNPA2/B1 transcripts were maintained in cholinergic-deficient mice or AD brains (Berson et al., 2012), but hnRNPA2/B1 translation was selectively decreased. Notably, hnRNPA2/B1 has been shown to be autoregulated by a RUST mechanism involving alternative splicing in its 3′ untranslated region that leads to NMD driven by mTOR1C (McGlincy et al., 2010; Dempsey, 2012), which is a key effector of muscarinic receptor signaling (Slack and Blusztajn, 2008). Correspondingly, we found that cholinergic control of hnRNPA2/B1 translation is mediated by M1 muscarinic receptors (Fig. 5G).
Targeting the interactions between RBPs and RNA may serve as a new potential therapeutic avenue to restore the RNA-editing deficits observed in neurodegenerative diseases (Tollervey et al., 2011; Berson et al., 2012; Bai et al., 2013; Qian and Liu, 2014). A number of substances, including regularly prescribed antibiotics, have been shown to nonselectively alter alternative splicing in the brain (Graveley, 2005; Tollervey et al., 2011; Kole et al., 2012); however, this approach lacks the ability to specifically target “impaired” RBP-RNA interactions.
Cholinergic failure is one of the hallmarks of AD, with the basal forebrain cholinergic system being heavily affected by the disease (Whitehouse et al., 1982). Data from the Alzheimer's Disease Neuroimaging Initiative has linked cholinergic failure in AD to both pathological outcomes (Teipel et al., 2014) as well as cognitive impairments in AD (Grothe et al., 2014). Further evidence for the importance of cholinergic signaling to the etiology of AD comes from clinical evidence that the long-term use of anticholinergic medication, specifically antimuscarinic drugs, significantly increases the risk of developing dementia (Gray et al., 2015). Interestingly, administration of antimuscarinic agents to AD patients exacerbates their symptoms (Lim et al., 2015). Together, these results suggest a crucial role for cholinergic tone in AD, with specific importance of muscarinic signaling.
The main pathological hallmarks of AD are the accumulation of Aβ plaques and of hyperphosphorylated tau (Huang and Jiang, 2009). M1 muscarinic signaling has been linked to both of these processes. Activation of the receptor has been shown to alter tau phosphorylation both in vitro (Sadot et al., 1996) and in vivo (Genis et al., 1999). Moreover, deletion of M1 muscarinic receptors increases Aβ related pathology in a transgenic mouse model overexpressing mutated APP (Davis et al., 2010). Furthermore, M1 agonists have been shown to reverse Aβ related pathology in mouse models of AD (Caccamo et al., 2006). These findings suggest that M1 receptors are key mediators of AD pathology. How hnRNPA2/B1 protein expression may contribute to pathology remains to be determined.
Cholinergic failure also plays an important role in one of the most important and apparent cognitive deficits of AD, memory loss (Bartus et al., 1982). There is a strong correlation between loss of basal forebrain cholinergic neurons and cognitive functioning in AD patients. Furthermore, mice with a forebrain specific deletion of VAChT have severe deficits in performance on the paired-associates learning touch-screen task (Al-Onaizi et al., 2016), a rodent version of the Cambridge Neuropsychological Test Automated Battery tests used in humans, which has been shown to be selective for the memory impairments in AD patients (Egerhazi et al., 2007). Importantly, lentiviral mediated knockdown of hnRNPA2/B1 also produced cognition impairments in mice (Berson et al., 2012).
Together, our findings indicate an intricate relationship between M1 muscarinic signaling and hnRNPA2/B1 translation. These findings lay the ground work for new therapeutic avenues for the treatment of AD. Specifically, they point to the potential of M1 muscarinic positive allosteric modulators to improve long-term changes in RNA metabolism and cognitive deficits due to cholinergic malfunction in AD. Noteworthy M1 muscarinic positive allosteric modulators have shown promising results to improve cognition in nonhuman primates (Lange et al., 2015).
Footnotes
This work was supported by the Canadian Institute of Health Research (MOP 136930, MOP 126000, and MOP 89919), Natural Sciences and Engineering Research Council of Canada (402524-2013), Brain Canada, the Canadian Foundation for Innovation, and the Ontario research fund (M.A.M.P., V.F.P.); the European Research Council (Advanced Award 321501) and the Legacy Heritage Science Initiative of the Israel Science Foundation (Grant 378/11) (H.S.); and an Annie Darkens Research Fund Award from the Alzheimer's Society of Canada Fellowship (B.K.). M.A.A.-O. received fellowship support from Kuwait University, and G.M.P. was a recipient of a Science without Borders fellowship from the Brazilian government. We thank Dr. Jurgen Wess, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, for providing M1 and M4 knockout mice.
The authors declare no competing financial interests.
- Correspondence should be addressed to either Marco Prado or Vania Prado, The University of Western Ontario, 1151 Richmond Street North, London, Ontario, Canada N6A 5B7. mprado{at}robarts.ca or vprado{at}robarts.ca