Figure 3. Pharmacologic inhibition of ROCKs reduces tau in neurons. Unless otherwise noted, n = 3 biological replicates per condition. Measurements of tau and mTOR phosphorylation were normalized to overall tau and mTOR levels, respectively. Statistics data for A, ii–iv, and C are located in Table 2. Ai, Representative blots are shown. Aii, Densitometry analysis of soluble tau, insoluble tau, and tau phosphorylation. Aiii, Densitometry analysis of autophagy-related markers after SR3677 treatment. Aiv, Densitometry analysis of LC3-II after SR3677 treatment. B, SR3677 reduced tau mRNA level (5 μm, 36%; 10 μm, 43%; 20 μm, 38%; 40 μm, 32%). n = 6 biological replicates per condition. C, Representative blots are shown. Densitometry analysis of soluble tau, insoluble tau, tau phosphorylation, and LC3-II levels after Fasudil treatment. D, Fasudil reduced tau mRNA level (20 μm, 22%; 40 μm, 24%). n = 6 biological replicates per condition. E, No gross changes in cell viability were observed at the indicated doses of Fasudil or SR3677 treatments. All data are expressed as the percentage of the mean ± SEM with respect to mock.