Article Figures & Data
Figures
- Figure 1.
Effects of long-term voluntary WR on body weight. a, Running distances (m/d) for the WR group were recorded and averaged between 4 and 24 months of age (n = 5–10). b, Body weights of WR and NWR groups were recorded and averaged (n = 5–10) between 4 and 24 months of age. c, Body weight of WR and NWR groups at 3, 6, 12, and 24 months of age (n = 5–10). Data are shown as means ± SEM. *p < 0.001 versus NWR.
- Figure 2.
Effects of long-term WR on tissue weights and blood glucose. The blood glucose level (g) and weights of liver (a), heart (b), lung (c), kidney (d), quadriceps (e), and gastrocnemius (f) were measured in NWR and WR groups at 6 or 24 months of age (n = 5–10). Data are shown as means ± SEM. *p < 0.05 versus NWR.
- Figure 3.
Effects of long-term WR on ABR (auditory brainstem response) hearing thresholds. a–f, ABR hearing thresholds from the 3-month-old NWR, 24-month-old NWR, and 24-month-old WR groups were measured at 4 (a), 8 (b), 16 (c), 32 (d), 48 (e), and 64 (f) kHz (n = 5–10). Data are shown as means ± SEM. *p < 0.05 versus 3-month-old NWR group. #p < 0.05 versus 24-month-old NWR group. g, ABR hearing thresholds at all frequencies from the 3-month-old NWR, 24-month-old NWR, and 24-month-old WR groups.
- Figure 4.
Effects of long-term WR on age-related hair cell loss. a, b, Cochleograms were recorded and averaged in the cochlear tissues of the young NWR (3-month-old), 24-month-old NWR, and WR groups (n = 5). The graphs show the quantification of cell loss in the IHCs (a) and OHCs (b). Data are shown as means ± SEM. *p < 0.05 versus 3-month-old NWR group; #p < 0.05 versus 24-month-old NWR.
- Figure 5.
Effects of long-term WR on SGN loss. a–i, Number of SGNs in each different region (apical, middle, and basal) of cochlea tissue from young NWR (3-month-old; a–c), old NWR (24-month-old, d–f), and old WR (24-month-old, g–i) groups (n = 4–5) was counted and quantified (j–l). Data are shown as means ± SEM. *p < 0.05 vs 3-month-old NWR; #p < 0.05 versus 24-month-old NWR. Scale bar, 25 μm.
- Figure 6.
GO term analysis. a, Average running distance per day of 6-month-old WR group, b, Body weight of NWR and WR groups between 3 and 6 months of age. Data are shown as means ± SEM. c, 529 BP categories displayed in a pie chart with seven subdivisions. d, 88 “Biological Regulation” categories displayed in a pie chart with seven subdivisions. e, 60 “Cellular Process” categories displayed in a pie chart with five subdivisions. f, 50 “Response to Stimulus” categories displayed in a pie chart with five subdivisions.
- Figure 7.
PCR array analysis. mRNA expression levels of 15 genes involved in inflammation were measured in the inner ears from the 6-month-old NWR (black bars) and WR (red bars) groups. Data are shown as means ± SEM. *p < 0.05 versus controls (NWR), Student's t test.
- Figure 8.
Effects of long-term WR on SV atrophy. a–i, Thickness of SV in each different region (apical, middle, and basal) of cochlea tissue from young NWR (3-month-old; a–c), old NWR (24-month-old; d–f), and old WR (24-month-old, g–i) groups (n = 4–5) was measured and quantified (j–l). Data are shown as means ± SEM. Scale bar, 25 μm. *p < 0.05 versus 3-month-old NWR; #p < 0.05 versus 24-month-old NWR.
- Figure 9.
Effects of long-term WR on age-related loss of strial capillaries. a–i, Numbers of endomucin-positive capillaries (red circles) in the SV in each different region (apical, middle, and basal region) of cochlea tissue from young NWR (3-month-old; a–c), old NWR (24-month-old; d–f), and old WR (24-month-old; g–i) groups (n = 4–5) were counted and quantified (j–l). Arrows indicate endomucin-positive capillaries in the SV. Data are shown as means ± SEM. Scale bar, 25 μm. *p < 0.05 versus 3-month-old NWR; #p < 0.05 versus 24-month-old NWR.
Tables
Mouse ID Running activity (m/d) 6 mo 12 mo 24 mo WR 1 11088 8904 4895 WR 2 14372 7713 3045 WR 3 11082 10781 6151 WR 4 10371 7441 3395 WR 5 12928 9794 3340 WR 6 10356 6879 2112 WR 7 12281 5606 3771 WR 8 13606 11185 4436 WR 9 14124 4752 4741 Mean 12245 8117 3987
