Figure 1. Coculture systems in CMDs. A, Schematic representation of CMD showing the neuronal soma cultured in a separate compartment (left) projecting axons toward the right compartment where different cell types can be cultured. B, Cocultures of DRG neurons (red) and osteoblasts (green). C, Cocultures of trigeminal ganglia neurons (TGG; left) and stem cells (right). Nuclear mitotic apparatus (NuMA) is stained with violet and βIII-tubulin (Tuj1; green). Adapted with permission from Pagella et al. (2015). D, Coculture of superior cervical ganglia neurons (SCG, left) and cardiomyocytes (right). E, Coculture of DRG neurons (green) and cancer cells (red). F, Coculture of CNS neurons (red) and glial cells (phase). Myelin basic protein (stained in green) and neurofilament (NF) (red). B, D, E, F, Adapted with permission from The Royal Society of Chemistry (Neto et al., 2014; Takeuchi et al., 2012; Lei et al., 2016; and Park et al., 2012, respectively).