Abstract
Differentiation and maturation of oligodendrocyte progenitor cells (OPCs) involve the assembly and disassembly of actin microfilaments. However, how actin dynamics are regulated during this process remains poorly understood. Leucine-rich repeat and Ig-like domain-containing Nogo receptor interacting protein 1 (LINGO-1) is a negative regulator of OPC differentiation. We discovered that anti-LINGO-1 antibody-promoted OPC differentiation was accompanied by upregulation of cytoplasmic gelsolin (cGSN), an abundant actin-severing protein involved in the depolymerization of actin filaments. Treating rat OPCs with cGSN siRNA reduced OPC differentiation, whereas overexpression of cGSN promoted OPC differentiation in vitro and remyelination in vivo. Furthermore, coexpression of cGSN and LINGO-1 blocked the inhibitory effect of LINGO-1. Our study demonstrates that cGSN works downstream of LINGO-1 signaling pathway, which enhances actin dynamics and is essential for OPC morphogenesis and differentiation. This finding may lead to novel therapeutic approaches for the treatment of demyelinating diseases such as multiple sclerosis (MS).
SIGNIFICANCE STATEMENT Myelin loss and subsequent axon degeneration contributes to a variety of neurological diseases, such as multiple sclerosis (MS). Understanding the regulation of myelination by oligodendrocytes is therefore critical for developing therapies for the treatment of MS. We previously demonstrated that leucine-rich repeat and Ig-like domain-containing Nogo receptor interacting protein 1 (LINGO-1) is a negative regulator of oligodendrocyte differentiation and that anti-LINGO-1 promotes remyelination in preclinical animal models for MS and in a phase II acute optic neuritis clinical trial (RENEW). The mechanism by which LINGO-1 regulates oligodendrocyte differentiation is unknown. Here, we demonstrate that LINGO-1 regulates oligodendrocyte differentiation and maturation through the cytoplasmic gelsolin signaling pathway, providing new drug targets for the treatment of demyelination diseases.
Introduction
Oligodendrocyte differentiation and myelination are tightly regulated processes that are essential to normal CNS development and function. Understanding how the processes are regulated should provide insights for developing new therapies for the treatment of demyelinating diseases such as multiple sclerosis (MS). Many factors have been implicated in regulating oligodendrocyte progenitor cell (OPC) differentiation and maturation (Bercury and Macklin, 2015). OPC differentiation requires dynamic assembly and disassembly of actin microfilaments to allow the plasma membrane extension necessary for lamellipodia/filopodia growth, membrane protrusion, and focal adhesions (Bercury and Macklin, 2015). Two recent studies suggest that actin dynamics are regulated in distinct manners for different phases of myelination: actin assembly is required for oligodendrocyte process extension, whereas actin disassembly leads to myelin wrapping (Nawaz et al., 2015; Zuchero et al., 2015).
Actin exists in cells in filamentous (F-actin) and globular/monomeric (G-actin) states. The ratio of F-actin to G-actin, which is tightly controlled by actin-binding proteins such as gelsolin (dos Remedios et al., 2003), regulates cell morphology and can change rapidly under physiological conditions. Gelsolin is an abundant actin-binding protein and plays a critical role in the regulation of actin dynamics (Li et al., 2012). Gelsolin exists in the extracellular (secreted, pGSN) and intracellular (cytoplasmic, cGSN) forms, which are expressed from an alternatively spliced gene under different promoters. pGSN contains an additional N-terminal 24 aa compared with cGSN (Kwiatkowski et al., 1986). Both forms of gelsolin are potent actin filament-severing proteins, converting F-actin to G-actin. pGSN plays an important role in the clearance of actin aggregates in the blood after tissue-injury-induced cell death (Lee and Galbraith, 1992; Spinardi and Witke, 2007; Bucki et al., 2008; Li-ChunHsieh et al., 2015). cGSN is ubiquitously expressed inside cells including myelin-forming oligodendrocytes. However, its function in OPC differentiation and myelination is unknown.
LINGO-1, a membrane protein selectively expressed in the CNS, has been identified as a negative regulator of oligodendrocyte differentiation and myelination (Mi et al., 2005; Mi et al., 2013). Blocking LINGO-1 function by antagonists including anti-LINGO-1 antibodies promotes oligodendrocyte differentiation and myelination in vitro and in vivo (Mi et al., 2007; Mi et al., 2009). Opicinumab (BIIB033), an anti-LINGO-1 antibody, improved visual evoked potentials in a phase II acute optic neuritis trial, suggesting a positive effect on remyelination (Cadavid et al., 2015). The downstream players that participate in the LINGO-1 signaling pathway remain unclear. Here, we report that cGSN plays a key role in oligodendrocyte differentiation, acting downstream of the LINGO-1 signaling pathway.
Materials and Methods
Quantitative RT-PCR.
RNA was isolated from cells using the Absolutely RNA Miniprep Kit (Agilent Technologies). Quantitative RT-PCR was used to quantify GSN and myelin basic protein (MBP) mRNA levels with β-actin used as an internal control. All primer sets were from Life Technologies, including custom-made cGSN and pGSN probes.
Human brain samples.
Twenty fresh-frozen brain samples from 11 postmortem human secondary progressive MS patients and 20 age- and sex-matched samples from patients with no neuropathological abnormalities were provided by University of Edinburgh (approved by East of Scotland Research Ethics Service). Tissue attributes were characterized by immunohistochemistry staining performed by University of Edinburgh (for details, see Table 1). Each sample was lysed with RIPA buffer (50 mm Tris, pH 7.2, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS, 150 mm NaCl, 10 mm MgCl2, 5% glycerol) at 1 ml per 0.2 mg of tissue. Equal amounts of protein were loaded onto 4–15% SDS-PAGE gel and subjected to Western blot analysis probed with antibodies against LINGO-1 (3C11, 2 μg/ml; Biogen), GSN (12953, RRID: AB-2632961, 1:1000; Cell Signaling Technology), or pGSN (NBP2-27566, RRID: AB-2632960, 2 μg/ml; Novus Biologicals). Western blot membranes were then stripped and reprobed with anti-GAPDH (NB600-502, RRID: AB-350715, 1:5000 or NB100-56875, RRID: AB-838305, 1:500; Novus Biologicals) as loading controls. Band intensities of the Western blot TIFF images were quantified by the Image Studio software (LI-COR).
Primary OPC culture.
Enriched populations of rat OPCs were purified as described previously (Mi et al., 2005) and maintained in a defined DMEM (DMEM with 4 mm glutamine, 1 mm sodium pyruvate, 0.1% w/v bovine serum albumin, 50 μg/ml apo-transferrin, 5 μg/ml insulin, 30 nm sodium selenite, 10 nm d-biotin, and 10 nm hydrocortisone) plus 10 ng/ml FGF and PDGF (Peprotech). For differentiation, the A2B5+ OPCs were cultured on PDL-coated plates (Fisher) in the defined DMEM supplemented with 15 nm T3 (Sigma-Aldrich) and 10 ng/ml ciliary neurotrophic factor (Peprotech) for 3 d with 2 μg/ml anti-LINGO-1 antibody (BIIB033; Biogen) or an aglycosyl IgG1 isotype control antibody (5C8; Biogen). All experiments related to OPC differentiation were repeated two to three times with two biological replicates for each experiment.
For transfection of plasmid or siRNA into OPCs, the electroporation method was used with an Amaxa kit (Lonza). Transfected OPCs were then plated onto PDL-coated plates in growth medium and then switched to differentiation medium for 3 d as described above. All siRNAs were from Life Technology, including custom-ordered cGSN and pGSN-specific siRNA. Rat GSN siRNA (ID# s150340), sense sequence (5′–3′) GGACAGUUUCAACAACGGUtt. Rat cGSN siRNA (ID# s529615), sense sequence (5′–3′) CCACAGCAGGUACCACCAAtt. Rat pGSN siRNA (ID# s529575), sense sequence (5′–3′) CAAAGUCGGGUGUCUGAGAtt.
For assessing protein expression by Western blot, cells were lysed in RIPA buffer and Western blots were performed as described above using antibodies to MBP (SMI-94 and SMI-99, RRID: AB-87330 and AB-2314772, 1:4000 mixture of each; BioLegend), MAG (34-6200, RRID: AB-87330, 1:1000; Life Technologies), myelin oligodendrocyte glycoprotein (MOG; MAB5680, RRID: AB-1587278, 1:10,000; Millipore), CNPase (ab6319, RRID: AB-2082593, 2 μg/ml; Abcam), GSN (12953S, RRID: AB-2632961, 1:1000; Cell Signaling Technology), phosphorylated-cofilin-1 (C8992, RRID: AB-1078547: 1:2000; Sigma-Aldrich), cofilin-1 (ab42824, RRID: AB-879739, 1:5000; Abcam), CAP-1 (ab155079, RRID: AB-2632962, 1:1000; Abcam), ArpC3 (612234, RRID: AB-399557, 1:1000; BD Biosciences), GAPDH (NB600-502, RRID: AB-350715, 1:5000; Novus Biologicals), and actin (4968S, RRID: AB-10695740, 1:1000; Cell Signaling Technology).
The G-actin/F-actin in vivo assay kit (BK037; Cytoskeleton) was used for the biochemical quantification of F-actin and G-actin amounts in OPCs. Briefly, cells were lysed in LAS2 buffer at 37°C for 10 min. After a 5 min room temperature centrifugation step at 350 × g to remove cell debris, the lysate were subjected to ultracentrifuge at 100,000 × g for 1 h at 37°C. The supernatant containing G-actin was removed and the pellet containing F-actin was solubilized to the equal volume with F-actin deploymerization buffer. Initial volumes of G-actin and F-actin fractions were analyzed by Western blot for actin. All reagents used in the assay were supplied by the kit. Band intensities of the Western blot TIFF images were quantified with Image Studio software (LI-COR).
Immunofluorescence.
All procedures were performed at room temperature. Cells cultured on chamber slides were fixed in 4% paraformaldehyde for 30 min, blocked, and permeablized in 10% normal goat serum and 0.1% Triton X-100 in PBS. MBP stainings were done by incubation with anti-MBP antibody (SMI-94 and SMI-99, RRID: AB-87330 and AB-2314772, 1:500; BioLegend) in blocking buffer for 2 h, followed by Alexa Fluor 488-labeled goat anti-mouse IgG (A11029, RRID: AB-2534088, 1:500; Life Technologies) in blocking buffer for 1 h. For F-actin staining, cells were incubated with Alexa Fluor 594-phalloidin (A12381, RRID: AB-2315633, 5 units/ml; Life Technologies) at room temperature for 1 h. Slides were mounted with Prolong Gold mounting media containing DAPI (P36931; Life Technologies). Images were taken using a Leica DMR epifluorescence microscope with 63× oil lens or Olympus VS120 slide scanner with 20× lens. Image quantification was done with Visiopharm software. For MBP quantification, MBP+ cells per image were counted or quantified by the total area of MBP+ fluorescent signal divided by the number of DAPI+ cells.
Proteomic analysis.
Primary rat OPCs cultured in differentiation medium in the presence/absence of 2 μg/ml anti-LINGO-1 antibody (BIIB033; Biogen) for 3 d were lysed in digestion buffer (50 mm HEPES, 150 mm NaCl, 1 mm EDTA, 4% Rapigest) by sonication. Cell lysates were centrifuged and supernatants were reduced, alkylated, and trypsin digested overnight as described previously (Ross et al., 2004). The resulting peptide mixtures were iTRAQ labeled and subsequently combined into six four-plex mixtures. Each of these four-plex mixtures was analyzed by 2D nanoliquid chromatography mass spectrometry with strong cation exchange and reverse phase as the first and second dimension, respectively.
Animal care.
All animal handling and surgical procedures followed the protocols approved by the Biogen Institutional Animal Care and Use Committee.
Mouse samples.
Heterozygous (Het) Shiverer mice were purchased from The Jackson Laboratory (https://www.jax.org/strain/001428) and bred in house as Het × Het. Mice were housed in cages of two to five animals in 12/12 h light/dark cycles. Fresh-frozen brain and spinal cord samples from P30 homozygous Shiverer mice and wild-type littermates (1 male and 1 female) were collected after perfusion with PBS, lysed in RIPA buffer and subjected to Western blot analysis as described above for human brain samples and primary OPC culture.
Lysolecithin (LPC)-induced demyelination rat model.
Nine-week old female Sprague Dawley rats (http://www.criver.com/products-services/basic-research/find-a-model/sprague-dawley-rat) were anesthetized by isoflurane inhalation. Body temperature was monitored and animals were kept on an electric heating pad during anesthesia. Aseptic technique was used throughout the procedure. AAV9 viruses (4.8 × 1010 genome copy in 20 μl) encoding cGSN or GFP were intrathecally injected into cisterna magna 14 d before LPC injection. LPC (4 μl of 1% in saline; L1381, Sigma-Aldrich) was injected into the dorsal column to induce demyelination in spinal cord. Animals were killed 9 d after LPC injection and the region of the spinal cord encompassing the lesion was excised and sectioned. Remyelinated axons in the lesions were quantified by toluidine blue staining of 1 μm transverse thin sections. Representative images of remyelinated axons were taken by electron microscopy. Studies were performed double blinded. cDNA encoding rat cGSN with C-terminal Flag tag was cloned into an AAV vector under the CAG promoter provided by Vector Biolabs. The production and titering of the AAV9-CAG-GFP or AAV9-CAG-cGSN viruses were performed by Vector Biolabs. GSN protein expression from the AAV-GSN viruses was confirmed by Western blot of transduced 293 cells as described above.
Statistics.
The data collection and analysis were performed blinded. GraphPad Prism 6 software was used for statistical analysis. Comparison of mean values was conducted with unpaired Student's t tests or one-way ANOVA. In all analyses, statistical significance was determined at the 5% level (p < 0.05). No data were excluded from the analyses. Data are presented as mean ± SD except for those shown in Figure 5B, which are shown as mean ± SEM. No statistical methods were used to predetermine sample sizes, which were chosen based on previous literature in the field. Data distribution was assumed to be normal, but this was not formally tested.
Data availability.
Original data are available from the corresponding author upon reasonable request.
Results
Gelsolin is upregulated in OPC differentiation
To unravel the molecular determinants of LINGO-1 function in oligodendrocyte biology, we performed a proteomic profiling study of primary rat OPCs. Anti-LINGO-1 antibody-induced oligodendrocyte differentiation was associated with increased protein expression of myelin components, such as MBP, proteolipid protein-1, and MOG. We discovered that GSN was also upregulated among the proteins most affected by anti-LINGO-1 treatment (Fig. 1A). We confirmed by quantitative PCR and Western blot analysis that anti-LINGO-1 antibody treatment increased GSN expression (Fig. 1B–D). When OPCs were cultured in differentiation medium without anti-LINGO-1, basal levels of GSN and MBP increased slightly as cells differentiated slowly over time. Anti-LINGO-1 antibody treatment accelerated the differentiation, as demonstrated by significant upregulation of MBP mRNA levels at 1 and 2 d. Notably, levels of GSN were also upregulated (Fig. 1B). By 3 d, MBP and GSN protein levels were increased 19- and 6-fold over control antibody-treated cells, respectively (Fig. 1 C,D). Immunocytochemistry (ICC) of the cultures revealed a correspondingly significant increase in the percentage of differentiated oligodendrocytes (Fig. 1E,F). Despite the effect on GSN, anti-LINGO-1 treatment had little or no effect on Arp2/3 complex subunit 3 (ArpC3) or adenylyl cyclase-associated protein (CAP-1), two ubiquitously expressed proteins that have been implicated in the control of actin dynamics. Interestingly, although the amount of cofilin-1 did not change (Fig. 1B,C), phosphorylated cofilin-1 increased approximately 4-fold upon anti-LINGO-1 treatment (Fig. 1C).
Because cGSN is an actin disassembly factor that functions through its capping and severing activities, we next determined whether anti-LINGO-1 treatment decreased F-actin microfilaments using fluorescent phalloidin staining (Zuchero et al., 2015). In control antibody-treated cultures, poorly differentiated oligodendrocytes with very few processes showed strong phalloidin labeling and poorly differentiated cell morphology. In contrast, anti-LINGO-1 antibody treatment led to strong MBP staining and poor phalloidin staining in processes (Fig. 1E). Levels of F- and G-actin in oligodendrocytes were further determined by biochemical separation and analyzed by Western blot. The F-actin/G-actin ratio in the cells treated with anti-LINGO-1 antibody was decreased by 40% versus control-treated cells, with no significant change in total actin (Fig. 1G–I). Therefore, OPC differentiation promoted by anti-LINGO-1 antibody correlates with a decrease in F-actin and an increase in G-actin levels, potentially reflecting the selective increase in GSN.
cGSN is required for OPC differentiation
Actin dynamics, triggered by actin depolymerizing proteins such as GSN, is a necessary step to promote actin disassembly/reassembly into new structures such as lamellipodia and filopodia, the formation of which is required for OPC differentiation (Bauer et al., 2009). To explore the role of GSN and actin disassembly in OPC differentiation, siRNAs against either total GSN or cGSN were tested and gave similar results. cGSN siRNA treatment significantly reduced both the mRNA and protein levels of GSN in OPCs (Fig. 2A,B, and mRNA data not shown). Strikingly, cGSN siRNA treatment inhibited OPC differentiation induced by anti-LINGO-1 antibody, reducing MBP protein expression by 78% (Fig. 2A,C). We also observed strong inhibition of membrane process formation by cGSN siRNA (Fig. 2D,E). This study suggests that cGSN is required for OPC differentiation. In contrast, pGSN-specific siRNA had no effect on OPC differentiation in the presence of anti-LINGO-1 antibody treatment (Fig. 2F) despite decreasing corresponding pGSN mRNA levels by ∼60% (Fig. 2G). Therefore, cGSN, but not pGSN, is required for OPC differentiation.
To further confirm the effect of cGSN on OPC differentiation, we overexpressed either cGSN or pGSN in OPCs by transfection. Overexpression of cGSN promoted cell differentiation. We observed a 10-fold increase in MBP protein production by Western blot and a 15-fold increase in the number of MBP+ myelin sheet-forming cells by ICC (Fig. 3A–D). In contrast, overexpression of pGSN did not promote OPC differentiation (Fig. 3A–D). We further confirmed the pGSN overexpression result by treating OPCs with purified pGSN protein. Similar to the pGSN overexpression data, pGSN protein treatment did not increase MBP expression (Fig. 3E) compared with control antibody treatment. This result further suggests that cGSN, but not pGSN, is involved in promoting OPC differentiation.
Previous studies have shown that LINGO-1 inhibits OPC differentiation (Mi et al., 2005). To determine whether cGSN functions downstream of the LINGO-1 OPC differentiation pathway, we looked at the effect of overexpressing cGSN and/or LINGO-1 on MBP expression. As shown in Figure 3F, overexpression of both LINGO-1 and cGSN at the same time induced MBP production to similar levels as seen in cells transfected with cGSN plasmid alone, indicating that overexpression of LINGO-1 did not block cGSN-induced OPC differentiation. In contrast, overexpression of both LINGO-1 and pGSN did not promote OPC differentiation. Together, our data showed that cGSN, but not pGSN, is required for OPC differentiation and that cGSN works downstream of LINGO-1.
cGSN is downregulated in Shiverer mice spinal cord and brain and human MS brain tissues
To further explore the connection between cGSN and myelination, we measured GSN levels in spinal cord and brain lysates of Shiverer mice, which have hypomyelination in CNS due to the lack of MBP protein (Chernoff, 1981). As expected, MBP protein was not detectable in spinal cord and brain lysates of Shiverer mice (Fig. 4A). As reported previously, we also observed lower levels of MOG and MAG in Shiverer mice and that CNPase levels did not change (Mikoshiba et al., 1980). cGSN levels were 2- to 3-fold lower in Shiverer mice spinal cord and brain than in wild-type littermates (Fig. 4A,B), which is consistent with our finding that cGSN expression correlates with oligodendrocyte differentiation and maturation.
Because LINGO-1 and cGSN levels showed an inverse correlation during oligodendrocyte differentiation, we examined MS lesions, in which LINGO-1 is upregulated, to determine whether cGSN is downregulated. For this purpose, we examined the protein levels of LINGO-1 and GSN from 20 MS (5 chronic lesions, 5 active lesions, 6 chronic active lesions, and 4 active/chronic active lesions as listed in Table 1) and 20 sex- and age-matched non-MS brain tissues by Western blot analysis. Consistent with our finding, LINGO-1 levels increased by 4-fold and cGSN levels decreased by ∼5-fold across all types of MS tissues compared with non-MS controls (Fig. 4C–F). pGSN levels in MS tissue were below the detection limit (data not shown).
cGSN overexpression promotes remyelination in vivo
Because cGSN is required for OPC differentiation in vitro, we next tested whether cGSN overexpression promoted remyelination in the rat LPC model in which LPC was injected into the spinal cord to induce local demyelination (Fig. 5). AAV virus containing genes for cGSN or GFP as a control were administrated to the animals 14 d before LPC injection. GSN expression in oligodendrocytes in LPC lesions was confirmed by immunohistochemistry (data not shown). Remyelination was evaluated by toluidine blue staining on 1 μm thin sections 9 d after LPC injection (Fig. 5A). As shown in Figure 5, AAV-cGSN-treated rats contained ∼1.7-fold more remyelinated axons than AAV-GFP-treated rats. This result is consistent with our in vitro data showing the critical role of cGSN in OPC differentiation and maturation.
Discussion
MS is caused by inflammation and demyelination in the CNS. Demyelinated axons continue to degenerate and the accumulative axonal loss in chronic lesions leads to irreversible disability for the MS patients. Current treatments focus on managing the inflammation to slow the relapse rate. There is a large unmet need for therapies that can restore myelination and repair the damaged axons (Kremer et al., 2015; Najm et al., 2015; Kremer et al., 2016; Mei et al., 2016; Thiruvalluvan et al., 2016; Yao et al., 2016). The reasons for remyelination failure in MS are poorly understood. The presence of significant numbers of OPCs within demyelinated lesions or plaques suggests that inhibitory mechanisms in the lesions block OPC differentiation and maturation, thereby preventing them from myelinating the damaged axons (Chang et al., 2000).
Oligodendrocyte differentiation from bipolar OPCs to mature oligodendrocytes involves dramatic morphological changes that are regulated by actin microfilament dynamics (Bauer et al., 2009). These morphological changes are closely linked to the myelination process. Recent studies suggest that actin assembly is first required for initial outgrowth of actin-rich structures, lamellipodia, and filopodia and then actin disassembly is required for myelin sheet formation and myelination (Nawaz et al., 2015; Zuchero et al., 2015). Our data are in agreement that actin disassembly is required for OPC differentiation and, more importantly, suggest that cGSN, a key regulator of actin dynamics, plays a critical role for myelin sheet formation.
Gelsolin is a capping and severing actin-binding protein. pGSN functions to scavenge actin circulating in blood after tissue injury (Lee and Galbraith, 1992) and has been shown to decrease actin toxicity and inflammation in a murine model of MS (Li-ChunHsieh et al., 2015). The role of cGSN in regulating OPC intracellular actin for cell morphology is largely undefined. In this study, we demonstrated that cGSN plays an important role for regulating OPC differentiation and maturation. cGSN overexpression facilitated OPC differentiation, whereas cGSN knockdown exerted an opposite effect. In Shiverer mice in which myelination was deficient due to lack of OPC terminal differentiation and maturation resulting from the lack of MBP protein (Seiwa et al., 2002), cGSN levels were also significantly reduced, along with other oligodendrocyte maturation markers (such as MOG and MAG). Conversely, the level of CNPase, which appears earlier than MBP (Reynolds and Wilkin, 1988; Pfeiffer et al., 1993), did not change. Therefore, increasing cGSN levels could facilitate OPC differentiation and myelination, which could be critical for remyelination of demyelinated axons in the MS lesion. Decreased myelin thickness of optic nerves was observed in GSN knock-out mice (Zuchero et al., 2015). In general, the lack of severe phenotypes in knock-out mice due to compensatory effects appears to be a quite common phenomenon (Barbaric et al., 2007). Therefore, the lack of a severe phenotype on CNS myelination in GSN knock-out mice might be due to compensatory effects during development from other actin disassembly factors such as cofilin (Kronenberg et al., 2010; Nawaz et al., 2015). In our study, the dramatic inhibitory effect of cGSN siRNA on OPC differentiation in vitro may be due to the lack of the compensatory factors in an acute setting in 3 d cultures.
LINGO-1 is selectively expressed in the CNS in both oligodendrocytes and neurons (Mi et al., 2013). LINGO-1 functions by binding to a number of receptors on the cell surface, leading to activation or inactivation of downstream signaling pathways in a cell-type-dependent manner. For example, LINGO-1 forms complexes with NgR1 and p75 or Troy in neurons to activate RhoA pathway to inhibit neurite outgrowth (Mi et al., 2004; Park et al., 2005; Shao et al., 2005). LINGO-1 binds to EGFR in dopaminergic neurons and to TrkB in retinal ganglion cells, which blocks the respective EGF or BDNF pathways for neuronal survival (Inoue et al., 2007; Fu et al., 2010). In oligodendrocytes, LINGO-1 binds to ErbB2 to inhibit OPC terminal differentiation (Lee et al., 2014). Anti-LINGO-1 antibody treatment increased OPC differentiation, which was associated with increased production of MBP and other myelin-associated proteins. By studying this process, we discovered that blocking LINGO-1 promotes cGSN expression. Like anti-LINGO-1 antibody treatment, overexpression of cGSN increased OPC differentiation in vitro and remyelination in vivo. In contrast, overexpression of LINGO-1 or blocking cGSN by its siRNA blocked OPC differentiation. Coexpression of cGSN and LINGO-1 overcame the inhibitory effect of LINGO-1. Intriguingly, we observed that cGSN levels were significantly reduced in MS brains, in which OPC differentiation is stalled in the lesions (Chang et al., 2000) and LINGO-1 is highly expressed. Our findings suggest that cGSN works downstream of the LINGO-1 signaling pathway. How cGSN expression is regulated by LINGO-1 remains to be determined.
In summary, we discovered that LINGO-1 negatively regulates oligodendrocyte differentiation through the cGSN signaling pathway, thus identifying a novel role of cGSN in oligodendrocyte differentiation and maturation. This finding provides new insights into potential drug targets for CNS remyelination that can be used for the treatment of demyelination diseases such as MS.
Footnotes
We thank Colin Smith and Chris-Ann McKenzie at University of Edinburgh for providing the fresh-frozen brain samples from human MS and non-MS patients for Western blot analysis, and Yun Bian for helping to harvest tissues from the animals.
The authors hold stock in Biogen, Inc.
- Correspondence should be addressed to Sha Mi, Ph.D., Department of Neurology Research, Biogen, Inc., 115 Broadway, Cambridge, MA 02142. sha.mi{at}biogen.com