Figure 1. RNA-Seq analysis of TRAP-isolated translating mRNAs from adult astrocytes in various brain regions of BAC aldh1l1 TRAP mice. A, Illustration of brain regions analyzed on a representative sagittal brain section. Ctx, Somatosensory cortex; hippo, hippocampus; thal, thalamus; hypo, hypothalamus; nacc, nucleus accumbens; cpu, caudate-putamen; DV axis, dorsoventral axis. Scale bar, 500 μm. B, Representative ALDH1L1 immunostaining in subcortical brain regions of BAC aldh1l1-TRAP mice (P70). Scale bar, 50 μm. White arrows indicate colocalization between ALDH1L1 immunostaining and eGFP fluorescence. C, Spearman rank correlation of sample replicates for each region. D, Principal component analysis plot of RNA-Seq results of all samples. Unique mRNA expression signature of cortex (E), hippocampus (F), caudate-putamen (Cpu, G), thalamus (H), nucleus accumbens (Nacc, I), and hypothalamus (J). Each region's astrocyte RNA-Seq dataset was compared with all other regions to identify the unique mRNA signature. q value, FDR-adjusted p value. q < 0.001 for Ctx and Hippo; q < 0.01 for all other regions. K, A partial Venn diagram with relatively enriched gene expression from each region's astrocytes. Values in yellow in parentheses are the number of genes that are enriched in each region's astrocytes. Values in italics and in parentheses are the number of genes that are shared from multiple (3) regions' astrocytes. Only intersection up to 3 regions is displayed, due to the large number of total intersections from 6 regions. FDR q value <0.01 was used to identify the genes that are relatively enriched in each region's astrocytes.