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Research Articles, Cellular/Molecular

Submillisecond Optogenetic Control of Neuronal Firing with Two-Photon Holographic Photoactivation of Chronos

Emiliano Ronzitti, Rossella Conti, Valeria Zampini, Dimitrii Tanese, Amanda J. Foust, Nathan Klapoetke, Edward S. Boyden, Eirini Papagiakoumou and Valentina Emiliani
Journal of Neuroscience 1 November 2017, 37 (44) 10679-10689; DOI: https://doi.org/10.1523/JNEUROSCI.1246-17.2017
Emiliano Ronzitti
1Neurophotonics Laboratory, Wavefront Engineering Microscopy Group, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 8250, Université Paris Descartes, 75270 Paris Cedex 06, France,
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Rossella Conti
1Neurophotonics Laboratory, Wavefront Engineering Microscopy Group, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 8250, Université Paris Descartes, 75270 Paris Cedex 06, France,
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Valeria Zampini
1Neurophotonics Laboratory, Wavefront Engineering Microscopy Group, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 8250, Université Paris Descartes, 75270 Paris Cedex 06, France,
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Dimitrii Tanese
1Neurophotonics Laboratory, Wavefront Engineering Microscopy Group, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 8250, Université Paris Descartes, 75270 Paris Cedex 06, France,
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Amanda J. Foust
1Neurophotonics Laboratory, Wavefront Engineering Microscopy Group, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 8250, Université Paris Descartes, 75270 Paris Cedex 06, France,
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Nathan Klapoetke
3Media Laboratory and McGovern Institute, Departments of Biological Engineering and Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, and
4Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147
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Edward S. Boyden
3Media Laboratory and McGovern Institute, Departments of Biological Engineering and Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, and
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Eirini Papagiakoumou
1Neurophotonics Laboratory, Wavefront Engineering Microscopy Group, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 8250, Université Paris Descartes, 75270 Paris Cedex 06, France,
2Institut National de la Santé et de la Recherche Médicale (INSERM), 75013 Paris, France,
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Valentina Emiliani
1Neurophotonics Laboratory, Wavefront Engineering Microscopy Group, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 8250, Université Paris Descartes, 75270 Paris Cedex 06, France,
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Abstract

Optogenetic neuronal network manipulation promises to unravel a long-standing mystery in neuroscience: how does microcircuit activity relate causally to behavioral and pathological states? The challenge to evoke spikes with high spatial and temporal complexity necessitates further joint development of light-delivery approaches and custom opsins. Two-photon (2P) light-targeting strategies demonstrated in-depth generation of action potentials in photosensitive neurons both in vitro and in vivo, but thus far lack the temporal precision necessary to induce precisely timed spiking events. Here, we show that efficient current integration enabled by 2P holographic amplified laser illumination of Chronos, a highly light-sensitive and fast opsin, can evoke spikes with submillisecond precision and repeated firing up to 100 Hz in brain slices from Swiss male mice. These results pave the way for optogenetic manipulation with the spatial and temporal sophistication necessary to mimic natural microcircuit activity.

SIGNIFICANCE STATEMENT To reveal causal links between neuronal activity and behavior, it is necessary to develop experimental strategies to induce spatially and temporally sophisticated perturbation of network microcircuits. Two-photon computer generated holography (2P-CGH) recently demonstrated 3D optogenetic control of selected pools of neurons with single-cell accuracy in depth in the brain. Here, we show that exciting the fast opsin Chronos with amplified laser 2P-CGH enables cellular-resolution targeting with unprecedented temporal control, driving spiking up to 100 Hz with submillisecond onset precision using low laser power densities. This system achieves a unique combination of spatial flexibility and temporal precision needed to pattern optogenetically inputs that mimic natural neuronal network activity patterns.

  • holography
  • optogenetics
  • two-photon excitation

Introduction

Optogenetic manipulation of neuronal excitation and inhibition (Boyden et al., 2005; Yizhar et al., 2011a) has the potential to increase exponentially neuroscience's capacity to elucidate causal relationships between neural circuits and behavior (O'Connor et al., 2009). Reaching this goal depends on developing methods to manipulate neurons in spatiotemporal patterns that mimic physiological neuronal network activity, which necessitates single-cell optical control of spiking at high firing rates with submillisecond temporal precision (Lin, 2011; Peron and Svoboda, 2011). These goals necessitate the parallel development of fast and highly light-sensitive opsins and sophisticated light-delivery approaches.

Recently engineered channelrhodopsin variants (Gunaydin et al., 2010; Berndt et al., 2011; Klapoetke et al., 2014) have improved light-gated channel kinetics significantly while maintaining high light sensitivity. Fast opsins such as ChETA and Chronos exhibit conducting state lifetimes nearly two to three times shorter than conventional ChR2 (Gunaydin et al., 2010; Klapoetke et al., 2014). Combined with single-photon (1P) wide-field illumination, these opsins enabled sustained and precisely timed firing over a range of frequencies from 5 to 200 Hz both in cultured neurons and acute brain slices (Gunaydin et al., 2010; Klapoetke et al., 2014), with major improvements in terms of spike reliability and reduction of artifactual spikes and plateau potential during extended light trains.

Due to the lack of axial confinement and limited penetration depth of 1P wide-field illumination, in depth, neuron-resolution optogenetic control requires confined soma-targeted two-photon (2P) illumination. 2P single-cell photostimulation can be achieved either by scanning a focused 2P laser beam (Rickgauer and Tank, 2009; Packer et al., 2012, 2015; Prakash et al., 2012) or by generating 2P-parallel illumination patterns using computer-generated holography (CGH), generalized phase contrast (GPC), and low-NA Gaussian beams combined with temporal focusing (Papagiakoumou et al., 2010; Bègue et al., 2013; Rickgauer et al., 2014; Chaigneau et al., 2016).

Scanning approaches rely mainly on slow opsins (i.e., C1V1; Yizhar et al., 2011b) with currents that temporally sum during the scan to reach spike threshold. Scanning thus necessitates a trade-off between scan time and temporal current integration of the light-gated channels serially illuminated across a cell. Evoking action potentials (APs) with scanning approaches required so far illumination durations ranging from 5 to 70 ms to achieve 6–20 ms AP onset jitter (Packer et al., 2012, 2015; Prakash et al., 2012) and AP rates up to 40 Hz (Prakash et al., 2012).

Parallel light-delivery approaches simultaneously actuate currents from every opsin in the cell membrane, integrating spatially to bring the neuron quickly to spike threshold. While scanning approaches necessitate slow-off kinetic opsins for temporal integration, parallel light delivery modalities enable spike with comparable latency and onset accuracy using either slow or fast opsins (Papagiakoumou et al., 2010; Bègue et al., 2013; Chaigneau et al., 2016). Parallel illumination of conventional ChR2 (Papagiakoumou et al., 2010) and slow opsins such as C1V1 (Bègue et al., 2013) and ReaChR (Chaigneau et al., 2016) have shown firing at up to 20–30 Hz spike rates. They are also susceptible to spike time variation and generation of spurious extra spikes. These shortcomings arise from opsins limited kinetics. Indeed, high-frequency, light-driven AP trains require opsins with rapid off kinetics for fast membrane repolarization and inactivation recovery after each spike.

These considerations indicate 2P parallel photostimulation of fast opsins as the optimal strategy for in-depth optical control of neural firing with high precise spatiotemporal precision. Here, we characterize the 2P action spectrum and kinetics of the fast opsin Chronos (Klapoetke et al., 2014) with holographically shaped light pulses. We demonstrate efficient current integration with 2P parallel illumination, enabling AP generation with submillisecond temporal precision and neuronal spike frequencies up to 100 Hz. Importantly, we used a fiber-amplifier, high-energy pulse laser to reduce average illumination power drastically. Our results highlight the potential of this approach to mimic a broad range of physiological firing patterns with submillisecond temporal precision, key to analyzing how specific patterns of network activity contribute to behavior and pathological states.

Materials and Methods

Imaging and photostimulation setup

The optical system was built around a commercial upright microscope (SliceScope; Scientifica) and combined a multi-light-path imaging architecture with a holographic-based photoactivation apparatus.

The imaging system was composed of three different imaging pathways: a 2P raster scanning, a 1P wide-field epifluorescence, and an infrared (IR) off-center oblique illumination imaging (see Fig. 1a).

2P imaging was provided by a mode-locked Ti-Sapphire source (Coherent Chameleon Vision II, pulse width 140 fs, tuning range 680–1080 nm). The femtosecond pulsed beam was raster scanned on the sample via a pair of XY galvanometric mirrors (3 mm aperture, 6215H series; Cambridge Technology) imaged at the back aperture of the microscope objective (40× W APO NIR; Nikon) through an afocal telescope (scan lens: f = 100 mm; Thorlabs #AC508-300-B; tube lens: f = 300 mm; Thorlabs #AC508-100-B). Galvanometric mirrors were driven by two servo drivers (MicroMax series 671; Cambridge Technology) controlled by a digital/analog converter board (PCI-6110; National Instrument). Emitted fluorescence was collected by a fiber-coupled detection scheme (Ducros et al., 2011) including a large diameter collector lens (f = 75 mm; Thorlabs #LB1309-A) and a 5-mm-diameter liquid light guide (Lumatec Series 300 customized with an f = 14.5 mm doublet lens glued at the fiber entrance by Till Photonics and an anti-reflective coating provided at the fiber exit). The fiber exit was imaged onto two photomultiplier tubes (PMT) GaAsP (H10770-40 SEL; Hamamatsu #H10770-40 SEL) by a set of three matching aspheres lenses (f = 23.5 mm; Melles Griot #LAG-32.5-23.5-C). After the fiber exit, fluorescence light was filtered by two IR-light blocking filters (Semrock #FF01-750sp), split into two channels by a dichroic mirror (Semrock #FF555-Di03) and detected through two emission filters (Semrock #FF01-510/84 and #FF02-617/73). 2P imaging laser power was tuned by combining an electrically controlled liquid crystal variable phase retarder (Meadowlark Optics #LRC-200-IR1) and a polarizer cube (Meadowlark Optics #BB-050-IR1).

1P imaging was provided by a LED source (Thorlabs #M470L2) filtered by a bandwidth excitation filter (Semrock #FF01-452/45) and coupled to a diffuser (Thorlabs #DG10-1500) and an achromatic lens (f = 30 mm, #LA1805 Thorlabs) to provide widefield illumination on the sample. 1P excited fluorescence was collected through a tube lens (f = 200 mm), separated from 1P excitation light using a dichroic mirror (Semrock #FF510-Di02) and detected by a charge-coupled device (CCD) camera (Hamamatsu Orca-05G) after passing through a visible bandwidth filter (Semrock FF01-609/181).

The collection of the fluorescence induced by 2P raster scanning or 1P wide-field illumination through either PMTs or CCD, respectively, is ensured by the presence in the fluorescence detection pathway of a downstream movable dichroic mirror (70 × 50 mm custom size; Semrock #FF705-Di01) and an upstream dichroic mirror (Chroma #ZT670rdc-xxrxt).

IR oblique illumination imaging was provided by an IR-LED source (Thorlabs #M780L2) installed at the rear port of the microscope coupled with a condenser focusing the light on the sample. IR light transmitted through the sample was collected with an IR CCD (DAGE-MIT IR-1000).

2P photoactivation was performed by generating arbitrary light shapes selectively matching specific opsin-tagged patterns in the sample via a spatially controlled phase modulation of the illumination beam wave front. In particular, a femtosecond pulsed beam delivered by a diode pumped, fiber amplifier system (Amplitude Systemes Satsuma HP; pulse width 250 fs, tunable repetition rate 500–2000 kHz, gated from single shot up to 2000 kHz with an external modulator, maximum pulse energy 20 μJ, maximum average power 10 W, wavelength λ = 1030 nm) operated at 500 kHz, was widened through an expanding telescope and reflected on the sensitive area of a reconfigurable liquid crystal on silicon spatial light modulator (SLM) (Hamamatsu Photonics LCOS-SLM #X10468-07). The reflected beam was projected at the back focal plane of the objective with an afocal telescope (f = 300 mm; Thorlabs #AC508-300-B and f = 500 mm Thorlabs #AC508-500-B). The SLM was controlled by custom-designed software (Lutz et al., 2008) based on a Gerchberg and Saxton iterative algorithm (Gerchberg and Saxton, 1972), which converts an arbitrary intensity pattern on the sample plane to a specific phase profile to be addressed at the SLM plane. The effect of the zero order of diffraction in the sample was suppressed by introducing a cylindrical lens (Hernandez et al., 2014).

2P imaging scan and 2P photoactivation beams were combined through a large dichroic mirror (50 × 70 mm; Chroma #T970dcspxr).

Intensity patterns matching the cellular surface were used for photoactivation of Chinese hamster ovary (CHO) cells, whereas holographich circular spots of 10–20 μm were used to photoactivate neurons. Power densities herein are given at the output of the objective and calculated referring to the excitation spot's surface.

Cell line culture and electrophysiology

CHO cells were cultured in an incubator at 32°C and 5% CO2 in a D-MEM/F12 GlutaMAX medium (Life Technologies) with the addition of 1 mm glutamine, 1% streptomycin, and 10% fetal bovine serum. Cells were plated on Thermanox plastic coverslips (Thermo Fisher Scientific) 24 h before transfection. Cells were transfected with a homemade plasmid (FCK-Gene90-GFP provided by Ed Boyden's laboratory) containing the Chronos opsin bound to GFP. The DNA was transfected using the ExGen 500 (Euromedex) transfection reagent and cells were recorded 24–48 h after transfection. Cultured cells were transferred for recording in a chamber mounted on the headstage of the microscope. Recordings were performed at room temperature. The extracellular medium during electrical recording contained the following (in mm): 140 NaCl, 5 KCl, 2 CaCl2, 1 MgCl2, 20 HEPES, and 25 glucose, pH adjusted to 7.5 by NaOH. Electrophysiological recordings were performed through a Multiclamp 700B Amplifier (Molecular Devices) in the whole-cell voltage-clamp recording configuration. Patch pipettes, pulled from borosilicate glass capillaries, had a resistance in the bath that ranged from 4 to 6 MΩs. Intracellular solution was composed of the following (in mm): 140 KCl, 2 MgCl2, 2 Mg ATP, 0.4 Na GTP, 10 HEPES, and 20 BAPTA, pH adjusted to 7.3 and osmolarity 330. Cells were maintained at −40 mV throughout recordings. The holding current ranged from 0.8 to 80 pA.

Stereotaxic injections of viral vector

All experimental procedures were approved by the Paris Descartes Ethics Committee for Animal Research (Registered Number CEEA34.EV.118.12) and followed European Union and Institutional guidelines for laboratory animals care and use (Council Directive 86/609 EEC). Stereotaxic injections of the viral vector rAAV8/Synapsin-ChR90-GFP (University of North Carolina Vector Core) were performed in 4-week-old Swiss male mice (Janvier laboratory). Mice were anesthetized with ketamine (80 mg/kg)–xylazine (5 mg/kg) solution and a small craniotomy (0.7 mm) was made on the skull overlying V1 cortex. Injection of 1–1.5 μl of solution containing the viral vector was made with a cannula at ∼80–100 nl/min at 200–250 μm below the dural surface. The craniotomy and the skull were sutured and the mouse recovered from anesthesia. The combination of our injection protocol and the viral vector used, resulted in a higher percentage of opsin-expressing interneurons with respect to pyramidal cells. Moreover, in the latter, the level of expression was relatively low, as can be seen from the data in Table 1. In pyramidal cells, we had to double the illuminated surface (surface diameter from 10–15 μm for interneurons to 15–20 μm for pyramidal cells) to obtain similar average photocurrents.

Acute slice preparation and electrophysiology

Acute parasagittal slices of the visual cortex were made from adult mice 6–8 weeks after viral injection. Animals were decapitated after being anesthetized with isofluorane. The brain was quickly removed and put in an ice-cold sucrose cutting solution containing the following (in mm): 85 NaCl, 65 sucrose, 2.5 KCl, 0.5 CaCl2, 4 MgCl2, and 25 glucose, pH 7.4, saturated with 95% O2 and 5% CO2.

The brain was dissected and 300-μm-thick slices were cut on a vibratome (Leica Biosystems VT1200S). Slices were maintained at 32°C for 30 min in standard ACSF (sACSF) containing the following (in mm): 125 NaCl, 2.5 KCl, 26 NaHCO3, 1.25 NaH2PO4, 1 MgCl2, 1.5 CaCl2, 25 glucose, and 0.5 ascorbic acid saturated with 95% O2 and 5% CO2 and then transferred at room temperature in the same solution until use. During the experiments, the slices were kept in a chamber and perfused continuously with fresh sACSF saturated with 95% O2 and 5% CO2. Cells were patched between 30 to 60 μm from the slice surface because more superficial cells are potentially damaged during slicing. The pipette solutions contained the following (in mm): 130 K-gluconate, 7 KCl, 4 MgATP, 0.3 GTP, 10 phosphocreatine-Na, and 10 HEPES, pH 7.2 with KOH. Borosilicate glass pipette resistance in the bath was 8–12 MΩs. Series resistances ranged between 20 and 40 MΩs (average: 30 ± 7 MΩs) and were compensated for by 60–70%. For interneuron recordings, input resistance ranged between 105 and 520 MΩs (average: 220 ± 100 MΩs), membrane capacitance ranged between 18 and 79 pF (average: 37 ± 15 pF), and membrane resting potential ranged between −54 and −76 mV (average: −65 ± 5 mV). For pyramidal cell recordings, input resistance ranged between 172 and 770 MΩs (average: 400 ± 200 MΩs), membrane capacitance ranged between 42 and 158 pF (average: 90 ± 40 pF), and membrane resting potential ranged between −70 and −82 mV (average: −76 ± 5 mV).

Neuronal type was established based on cell morphology under IR oblique illumination and their firing properties upon suprathreshold current injections of increasing amplitudes up to 350 pA in the current-clamp recording configuration. Cells were inspected visually before patch with a 40× water-immersion objective. The characteristic AP firing was the main parameter used during recordings to distinguish the type of cells. The firing of pyramidal cells shows a classic spike frequency adaptation and the maximal frequency of firing is close to 20 Hz; the second AP in a series is wider than the first, and the development of an adaptive hump as the cell is further depolarized can be often observed. Most interneurons were fast spiking, which show almost no adaptation in their firing, their AP characterized by a big component of the fast after-hyperpolarization and with frequencies well beyond 20 Hz (Cauli et al., 2000). A few cases (n = 3) were regular spiking nonpyramidal or stuttering interneurons.

Photostimulation procedure

Two different approaches were used to search for cells expressing the Chronos opsin. In most of the experiments expression was sparse and it was then relatively simple to detect single-transfected cells through wide-field fluorescence illumination. In cases of higher expression, in which the background fluorescence was too high, we used the 2P imaging. In regions of really high levels of expression, we instead adopted the option of blindly patched cells, and their level of expression was verified by electrical recordings of the currents evoked by photostimulation. Recorded cells were monitored through IR oblique light illumination during the patching phase.

Photostimulation of CHO cells.

2P photocurrents were evoked while maintaining the cell in voltage-clamp configuration at −40 mV. For the characterization of the current response as a function of the light power density, a holographic shape completely covering the cell surface was used. In this way, all channels fell within the illuminated area and we could avoid problems of spurious recruitment of channels outside of the holographic pattern as the power increases.

The experiments to characterize the spectral response of Chronos were performed on a different setup equipped with a tunable Ti:Sapphire laser and with a GPC holographic illumination delivering large-diameter spots that covered the whole cell (Papagiakoumou et al., 2010; Chaigneau et al., 2016).

Photostimulation of neuronal cells.

Recorded cells were photostimulated with 2P holographic spots of 10–20 μm diameter centered on the soma of the cell. The duration and intensity of the stimulus varied depending on the experimental procedure and are indicated in the results. The value of the power density used during the stimulation was calculated as the ratio of the laser power measured after the objective and the surface of the designed spot.

Data acquisition

For the experiments on spectral characterization, electrical signals were recorded through a Multiclamp 700B patch-clamp amplifier and digitized using a Digidata 1322A interface and pClamp software (Molecular Devices). All other recordings were performed using a Multiclamp 700B patch-clamp amplifier and digitized using a National Instruments data acquisition board (#USB-6259). NeuroMatic software environment in conjunction with IgorPro software (Wavemetrics) were used for data acquisition and analysis. Results are quoted as mean ± SD unless otherwise specified in the text. Voltage- and current-clamp recordings were filtered at 10 kHz and sampled at 50 kHz.

Results

Photophysical properties of Chronos under 2P illumination

To determine Chronos kinetic parameters and wavelength sensitivity under 2P, we used CHO cultured cells transfected with a plasmid encoding GFP-tagged Chronos. We used extended 2P holographic patterns (Lutz et al., 2008; Papagiakoumou et al., 2008) shaped over the cell's surface to photoactivate Chronos-expressing cells and whole-cell patch-clamp recordings to measure the light-induced ionic currents (Figure 1a, inset). A high-energy pulse, ultrafast fiber amplifier laser at λ = 1030 nm (see Materials and Methods for details) acted as the photostimulation source, allowing illumination at high peak powers and low average powers.

Figure 1.
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Figure 1.

Chronos 2P kinetics and spectra. a, 2P holographic photostimulation and 2P scanning imaging optical setup. PC, polarizer cube; λ/2, half-wave plate; M, mirror; L, lens; CL, cylindrical lens; D, dichroic; LC-VR, liquid crystal variable retarder; GM, galvanometric mirrors; F, filter; OBJ, objective; C, condenser. Inset, IR-transmitted (left) and 2P fluorescence (right) images of Chronos-expressing CHO cells. A patched cell is photostimulated by a holographic illumination pattern matching its surface (red pattern). b, Normalized peak photocurrent (n = 6 cells) over different illumination power densities. Inset, Representative photocurrents generated by irradiating a Chronos-expressing CHO cell with different illumination power densities (p = 0.04, 0.06, 0.09, 0.1, 0.12, 0.14 mW/μm2; Δt = 4 ms; λ = 1030 nm). c, d, On kinetics (c; measured as time to 90% of the peak) and off kinetics over different illumination power densities (d; n = 4 cells; different symbols and colors correspond to different cells). e, Chronos 2P action spectra (data are shown as mean ± SEM; n = 4 cells). Inset, Representative photocurrents generated by irradiating a Chronos-expressing CHO cell with different illumination wavelengths using equal photon flux.

Initially, we studied the amplitude and kinetics of the photocurrents elicited by photostimulating with short light pulses of varying power densities. The dependence of the photocurrent peak on the illumination power density shows the typical behavior characterized by a nonlinear regime at low power and a saturation phase at high power (>0.15 mW/μm2) (Rickgauer and Tank, 2009; Prakash et al., 2012; Chaigneau et al., 2016) (Fig. 1b). Interestingly, the fast photo cycling of Chronos leads to saturation power levels nearly three times higher than for the slow opsin ReaChR (Chaigneau et al., 2016). As reported previously under 1P activation (Klapoetke et al., 2014), the temporal dependence of Chronos 2P photocurrents reveals unprecedented fast on and off rates (Fig. 1c,d). The 2P turn-on time (τon) diminishes by increasing the illumination power (Fig. 1c) and reaches a value of 1.2 ± 0.1 ms (n = 4 cells) at powers near the saturation regime, whereas τoff is almost independent of the illumination power and equal to 3.8 ± 0.2 ms (Fig. 1d).

We then examined Chronos spectral responses by measuring the amplitude of the photocurrents elicited at various illumination wavelengths (Fig. 1e). In this case, we used an optical system equipped with a tunable Ti:Sapphire laser as photostimulation source combined with GPC to generate illumination spots entirely covering the cell (see Materials and Methods) (Papagiakoumou et al., 2010; Chaigneau et al., 2016). Photon flux was maintained constant across wavelengths for each cell, ranging between 1.7 and 2.4 × 1026 photons/m2s. Within the range of tunable wavelengths (750–1000 nm), the 2P action spectrum shows an absorption bandwidth between 850 and 1000 nm, peaking at 975 nm (Fig. 1e).

2P chronos spiking

To assess the spiking fidelity of Chronos-expressing neurons under 2P illumination in brain slices at various irradiances and frequencies, we injected layer 2/3 of visual cortex of 4-week-old Swiss mice with adeno-associated virus rAAV8/Synapsin-Chronos-GFP. Slices were prepared 6–8 weeks after injection. We photoactivated Chronos-expressing neurons by illuminating the soma of the cells with 2P holographic circular spots (10–15 μm spot diameter; λ = 1030 nm, fiber amplifier laser system). We recorded cellular activity by whole-cell patch clamp (Fig. 2a).

Figure 2.
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Figure 2.

2P activation of Chronos-expressing interneurons. a, 2P image of a portion of an acute cortical brain slice of a virus-injected mouse. A patched/Alexa Fluor 594-filled (white) interneuron of layer 2/3 expressing Chronos-GFP (green) is photostimulated by a 10 μm holographic spot (red spot). Scale bar, 20 μm. b, c, Representative light-evoked photocurrents (b) and membrane potential depolarization (c) over different illumination powers in a Chronos-expressing interneuron. d, Light-evoked currents in spiking (black) and nonspiking (red) neurons for power densities between 0.05 and 0.12 mW/μm2. Each circle corresponds to one cell. e, f, Latencies (e; calculated from the start of the light pulse to the spike depolarization threshold) and jitter (f; calculated as mean deviation of latency) of light-evoked spikes (n = 24 cells) by 10- to 15-μm-diameter spots (data are shown as mean ± SD). Red lines connect data from same cells.

To demonstrate the achievable temporal precision at high firing rates, we mainly focused the analysis and recordings on fast spiking interneurons. Firing properties of slow-spiking cells (pyramidal) were also investigated (Table 1).

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Table 1.

Resuming currents and spiking probability of Chronos-expressing neurons photoactivated by illuminating the cellular soma with circular 2P holographic spots

In voltage clamp, photocurrents of hundreds of picoamperes were evoked upon somatic photostimulation at moderate power density levels (I = 415 ± 213 pA, p = 0.08 ± 0.02 mW/μm2; mean ± SD on n = 38 interneurons; Fig. 2b,d). In current clamp, generation of single APs was obtained using 2–3 ms irradiation durations (Fig. 2c; cells successfully photoeliciting single AP: 74% for p < 0.12 mW/μm2; 84% for p < 0.3 mW/μm2, n = 38 interneurons). In a few cases of highly expressing cells (peak photocurrent in voltage clamp I = 817 ± 315 pA, p = 0.08 ± 0.02 mW/μm2, n = 5 cells), we could shorten the stimulation pulse and still obtain AP generation with 1 ms (n = 2 cells, APs evoked in 3/3 photostimulation trials per cell) and 0.7 ms (n = 3 cells, APs evoked in 3/3 photostimulation trials per cell) pulse duration (p = 0.13 ± 0.05 mW/μm2). APs occurred with short latencies (2.83 ± 0.97 ms, p = 0.11 ± 0.03 mW/μm2; mean ± SD. on n = 24 cells, Fig. 2e) and submillisecond temporal precision (jitter 0.19 ± 0.16 ms, p = 0.11 ± 0.03 mW/μm2; mean ± SD. on n = 24 cells, Fig. 2f). Increasing the illumination power enabled reducing spike latency and jitter (see line-connected cells in Fig. 2e,f).

The ability to evoke precisely timed, high-frequency spike trains demands that the following conditions are satisfied: short light-evoked AP latency, small jitter, and opsins featuring fast off kinetics and recovery from inactivation. In particular, light-evoked AP latency, determined in large part by the optical stimulation method (i.e., scanning vs holography) imposes a hard limit on the maximum spike actuation frequency. In practice, 100 Hz spike actuation requires latencies well <10 ms. The light-gated channel off time determines the minimum delay between two light-evoked APs necessary to fully repolarize the neuron. Recovery from inactivation determines the time necessary to make all opsins available again after a photostimulation pulse. These minimum delays guarantee that the neuron responds to each photostimulus in the very same manner, thus ensuring consistent responses and avoiding artifactual spike doublets or plateau potentials. Finally, the light-evoked AP jitter determines the time precision of light-driven firing. Our holographic actuation of Chronos is the first 2P optical system to satisfy all of these conditions.

To verify that high-frequency precisely timed spiking can be driven in Chronos-expressing neurons under 2P holographic illumination, we photostimulated with trains of light pulses (2–3 ms pulse width) at different frequencies neurons that successfully elicited a single AP. We found that 2P light stimulation could drive cells to spike up to a frequency of 100 Hz (Fig. 3a). 2P-driven spiking (with no failures) was achieved up to a stimulation frequency of 50 Hz, whereas spike failures might be encountered at 100 Hz stimulation frequency after 3–4 pulses depending on the Chronos expression level and the cell type (Fig. 3b). Contrary to what was obtained for slower opsins (Chaigneau et al., 2016), we observed only small plateau potentials even at high stimulation frequencies, indicating fast repolarization between illumination pulses (Gunaydin et al., 2010; Fenno et al., 2011). Furthermore, no asynchronous spikes were evoked and a modest increase of spike latency and jitter throughout the succession of photostimulations was recorded (Fig. 3c,d). Both observations confirm the high spike temporal precision of Chronos when driven by 2P patterned light during sustained stimulation of short light pulses. Importantly, due to the short pulse duration and relatively small amount of light necessary to trigger an AP, a prolonged series of photostimulations did not induce physiological damage detectable with whole-cell patch clamp. In a series of experiments (n = 5 cells), we tested cell stability during several minutes of successive series of 10 light pulses delivered at 10 Hz every 6 s. The AP amplitude, input resistance, and membrane potential did not change appreciably during 40 consecutive train stimulations (average AP amplitude: 99 ± 4 mV; AP amplitude variation: 0.8 ± 0.4 mV; input resistance: 143 ± 17 MΩ; input resistance variation 0 ± 6%; membrane potential variation: 0.9 ± 0.8 mV; Fig. 3e). Spike latencies showed modest augmentation during successive trains of photostimulation, suggesting that the proportion of inactivated channels gradually increased during photostimulation sustained over minutes (Fig. 3f).

Figure 3.
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Figure 3.

Light-driven spiking fidelity across different frequencies in Chronos-expressing interneurons. a, Sample trains of 10 photostimulation pulses of 2 ms duration each over different frequencies: 10 Hz, p = 0.09 mW/μm2 (bottom); 50 Hz, p = 0.09 mW/μm2 (middle); 100 Hz, p = 0.12 mW/μm2 (top). b, Spike probability within the 10 pulses (cells have been photostimulated either with four or 10 light-pulse trains; red shows the number of cells in which the i-th spike has been tested). c, d, Latency (c) and jitter (d) of each light-evoked spike of the train. Illumination power range: 0.05–0.17 mW/μm2, 2–3 ms illumination pulse duration, 10–15 μm diameter spot, λ = 1030 nm. Data are shown as mean ± SD. e, Trains of 10 pulses at 10 Hz triggered every 6 s after 5 min (black line) and after 15 min (blue) preceded by resistance steps. f, Latencies of each light-evoked spike within the train after 0 min (black) and 3.5 min (red; Top, n = 5 cells) and 0 min (black), 3.5 min (red), and 7 min (blue; bottom, n = 3 cells).

To assess the importance of fast kinetics for precisely timed, high-frequency spike stimulation, we compared the spiking performance of holographically actuated Chronos-expressing neurons with neurons expressing the slower opsin CoChR (τon ≈ 2.4 ms and τoff ≈ 30 ms; Papagiakoumou et al., 2017) under the same experimental conditions. We injected layer 2/3 of visual cortex of 4-week-old Swiss mice with adeno-associated virus AAV8/Synapsin-CoChR-GFP. In the CoChR-expressing interneurons, 1–3 ms illumination pulses produced single AP spike latency and jitter comparable to those of Chronos (CoChR AP latency: 4.76 ± 0.57 ms, AP jitter: 0.15 ± 0.05 ms; p = 0.09 ± 0.03 mW/μm2; mean ± SD. on n = 4 cells), corroborating the fact that, under 2P-CGH photostimulation, short light-evoked AP latency and jitter can be enabled independently both in fast and slow opsins (Chaigneau et al., 2016). We then assessed photostimulation with high-frequency illumination pulses. We could evoke spiking up to 50 Hz with high fidelity (Fig. 4a–d), although with higher plateau potential compared with Chronos. The spike rate could be increased above 50 Hz by increasing the frequency of the light stimulation train. However, depending on illumination power and pulse duration, light-driven spiking fidelity appeared distorted, exhibiting low synchronicity between the light pulses and spiking, missed APs, generation of extra spikes or plateau potentials, all indicating insufficient repolarization between consecutive pulses. In particular, tuning neither the illumination power nor the pulse duration could control spike timing and firing fidelity precisely (Fig. 4e). The analysis of the same type of photostimulation conducted in CHO cells further emphasized the limitations of high-frequency light-driven photostimulation combined with slow turn-off opsins. In CoChR-expressing CHO cells, photo-evoked currents decayed to zero between light pulses only for low-frequency stimulations (10 Hz), whereas significant current summation resulted for consecutive stimuli for higher photostimulation rates (50 and 100 Hz; Fig. 4f). Conversely, photocurrents evoked during high-frequency light-train stimulation of Chronos-expressing CHO cells decayed rapidly to baseline levels after each illumination without significantly affecting the following stimuli (Fig. 4f).

Figure 4.
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Figure 4.

Light-driven spiking fidelity across different frequencies in CoChR-expressing interneurons. a, Representative light-driven firing induced by a train of 10 illumination pulses at (top) 10 Hz (p = 0.1 mW/μm2; pulse width 2 ms; spot diameter 15 μm) and (bottom) 50 Hz (p = 0.12 mW/μm2; pulse width 1 ms; spot diameter 15 μm). b, Spike probability within the 10 pulses for illumination at 10 Hz (top) and 50 Hz (bottom; n = 4). c, d, Latency (c) and jitter (d) of each light-evoked spike of the train. Illumination power range: 0.05–0.12 mW/μm2, 1–3 ms illumination pulse duration, 15 μm diameter spot, λ = 1030 nm. Data are shown as mean ± SD (n = 4). e, Representative light-driven firing induced by a train of 10 illumination pulses at 100 Hz using different power and pulse width durations. f, Representative normalized photocurrents evoked illuminating with five pulses (2 ms pulse width) at different frequencies Chronos-expressing (black) and a CoChR-expressing (blue) CHO cells (top: 10 Hz, middle: 50 Hz, and bottom: 100 Hz).

Finally, because combining optical photostimulation with 2P-scanning Ca2+ imaging is a practice of wide interest for optogenetic studies, we investigated the activity induced on Chronos-expressing interneurons during 2P scanning of a large field of view (FOV). We performed whole-cell patch-clamp recordings of membrane potential variations in Chronos-expressing interneurons during 2P raster scanning of a 920 nm femtosecond beam typically used for imaging genetically encoded calcium indicators (GCaMPs). In particular, we tested cell depolarization while performing 2P scanning imaging (Fig. 5) using two FOVs (294 × 294 μm2 and 147 × 147 μm2) at different frame rates obtained by maintaining constant time per line and varying the number of lines (n = 6 cells). Cell depolarization augments by increasing the illumination power and the number of lines (i.e., reducing the interline distance and thus augmenting the sampling density of the scanned beam on the cell) or reducing the imaging FOV (i.e., increasing the fraction of time during which the scanned beam illuminates the cell and thus augmenting the duty-cycle of the scanned beam on the cell). Although the majority of cells showed modest depolarizations (<5 mV), significantly higher depolarizations were observed under certain illumination conditions in two of six interneurons. Although Chronos's fast off-kinetics may render it less vulnerable to spurious actuation during imaging scans compared with slower opsins, these results indicate that particular care is still needed when imaged with standard galvanometric mirror scanning regimes for calcium indicators such as GCaMP. Further work is needed to minimize such artifacts, in particular to identify high signal-to-noise fluorescence reporters with 2P spectra that overlap less with that of Chronos.

Figure 5.
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Figure 5.

Scanning imaging effects on Chronos-expressing interneurons. a, 2P image of a patched Chronos-exressing neuron of acute cortical brain slices acquired by raster scanning a 920 nm femtosecond beam (FOV 294 μm; 1.15 μm/line; 2.96 Hz frame rate; p = 14 mW; top); corresponding membrane potential depolarization is shown at bottom. b–e, Membrane depolarizations obtained on different cells by raster scanning a 920 nm beam over 294 μm (b, d) and 147 μm (c, e) with 14 mW (b, c) and 5 mW (d, e) excitation power for various line-scanning resolutions at 1.32 ms/line.

Discussion

We have demonstrated optical control of neuronal firing up to 100 Hz with submillisecond AP onset precision in acute cortical slices with soma-targeted 2P holographic illumination.

Millisecond temporal control of neuronal firing opens the door to spatially and temporally mimicking neural activity patterns, which is critical for studying how spike temporal and rate codes convey information in the brain (Lisman, 1997; Izhikevich et al., 2003; Baranauskas, 2015), refine reverse-engineering neural circuits programs (O'Connor et al., 2009), as well as closed-loop and activity-guided perturbation of neural dynamics and behavior (Grosenick et al., 2015). Precise spike timing and high-frequency neural events have been linked to many relevant phenomena in neural plasticity, behavior, and pathology, including setting the direction of synaptic change in spike-timing-dependent plasticity (Dan and Poo, 2004), mediating neural coding in systems such as the auditory system (Köppl, 1997), and contributing to functions altered in diseases such as Parkinson's disease (Foffani et al., 2003). Some cell types such as parvalbumin-positive interneurons fire at high frequencies and the loss of such neurons may be a contributor to psychiatric illness (Behrens et al., 2007). Fast patterns of neural spiking may also govern whether spikes propagate throughout dendrites and thus affect localized cellular processes (Buzsáki et al., 1996).

The achieved high temporal precision depends on a combination of factors inherent to the opsin Chronos in combination with the holographic light-shaping approach. The ability to drive high-frequency firing optically without introducing spurious artifacts (i.e., spike multiplets or plateau potentials) arises from two main factors: (1) the light-evoked AP latency, which determines how quickly an opsin-expressing neuron can be depolarized to spike threshold; and (2) the opsin's off kinetics and inactivation recovery, which determine the time necessary for the neuron to fully repolarize and for all the actuated opsin to recover. Accordingly, short light-evoked AP latency must be reached and fast kinetics opsins adopted. 2P holographic stimulation achieves well the first condition because of spatial summation of current evoked throughout the soma. Moreover, parallel illumination such as 2P-CGH is required to actuate spiking in opsins satisfying the second condition because such fast off kinetics likely preclude the temporal summation needed for point-scanning approaches. By reducing light-evoked AP latency and adopting fast kinetic opsins, the natural physiological firing limits of a specific neuron type can in principle be actuated optically. A third fundamental parameter, the jitter of light-evoked APs, describes the AP timing precision and thus the temporal control accuracy of light-driven firing. Here, we evoked spikes holographically with millisecond latency and submillisecond jitter for both Chronos- and CoChR-expressing targeted neurons. In addition, in the case of Chronos, holographic stimulation could actuate spiking up to 100 Hz because of this opsin's short kinetics.

The low power densities needed for high temporal precision 2P Chronos activation were enabled by the use of a fiber-amplifier low repetition rate laser. The fiber-amplifier laser source (500 kHz) achieves higher 2P efficiency than typical mode-locked Ti:Sapphire laser oscillators (typically 80–100 MHz) due to lower pulse repetition rates of the femtosecond beam (2P excited signal, S ∝ Pavg2 (fτ)−1, where f is the repetition rate, τ the pulse width, and Pavg the average beam power; Xu and Webb, 1996; Zipfel et al., 2003). This enabled efficient photo current and spike generation with modest light powers (<0.15 mW/μm2; 12–27 mW/cell) and short light pulses (<3 ms), allowing repetitive cell stimulation without affecting electrophysiological parameters. These findings, together with the fact that amplified lasers can deliver high average power beams (≈10 W at laser output), indicate that laser power is not the limiting factor for the maximum achievable number of targets using 2P-CGH.

These results open the door to in vivo light-targeted multineuronal activation with high temporal control and cell-targeted patterned stimulation of entire neuronal networks. The maximum number of neurons that can be stimulated simultaneously will be mainly limited by two elements: the light-induced heating and the deterioration of the photostimulation spatial resolution. Data shown here demonstrate that, in the proposed activation conditions, individual stimulated cells conserve physiological integrity even under sustained train of illuminations, suggesting that thermic effects per cell are kept below damaging threshold. Nevertheless, cumulative heating effects all over the sample need to be evaluated carefully when large and dense groups of neurons are stimulated. Effects of heating can be further compounded by additional light exposure during functional imaging. Further limitations for large-scale multiple-cell stimulation arise from the photostimulation of cellular processes crossing the overall illumination volume. Indeed, illumination of multiple processes of opsin-expressing neurons could likely generate APs in nontargeted cells, thus limiting the maximum number of targets that can be stimulated by maintaining cellular resolution. The use of opsins with expression that is confined genetically to the somatic region of the neurons can provide a critical advantage for excitation of large pools of neurons without spurious activation of nontargeted cells (Shemesh et al., in press; Baker et al., 2016).

It is important to underline that 2P holographic-based illumination patterns are robust to scattering and well conserve the intensity patterns distribution up to a few hundred microns in depth (Bègue et al., 2013). This ensures that the activation of targeted neurons under 2P-CGH-based illumination will permit obtaining analogous neuronal spiking reliability and temporal control at various depths by simply providing enough illumination power to compensate scattering losses.

Efficient current integration achievable under 2P parallel photostimulation enables millisecond spike generation and submillisecond temporal jitter using opsins with significantly slower kinetics (Chaigneau et al., 2016) than Chronos. However, in this case, the slow off kinetics cause a slow repolarization between pulses with consequent extra spikes and pronounced prolonged plateau depolarization, thus significantly hampering the fidelity of high-frequency light-driven spiking (Chaigneau et al., 2016). CoChR exhibits off kinetics nearly 10 times longer than Chronos and our comparison between these opsins in cortical interneurons underlines the importance of short off kinetics for fast, repetitive AP actuation. While CoChR-expressing neurons could exhibit various artifactual responses when photostimulated with high-frequency trains of light, the fast kinetics of Chronos enabled submillisecond timed control of light-driven neural firing up to 100 Hz, with no extra spikes and small plateau potential. Both latency and jitter values were impressively well maintained during high-frequency (up to 100 Hz) trains.

We expect that performance similar to the one achieved with Chronos and 2P holography can be reached by alternative parallel patterning approaches as GPC (Papagiakoumou et al., 2010) or low-NA Gaussian beams (Rickgauer et al., 2014) and/or other fast opsins such as ChETA variants (Gunaydin et al., 2010), although in this last case the inferior light sensitivity (Lin, 2011) will probably require using higher excitation power levels. Also note that the 2P action spectrum of Chronos revealed that 2P evoked currents under 1030 nm excitation are close to 60% of the absorption peak (@950 nm), making this opsin compatible with stimulation by high-energy amplified lasers emitting around this wavelength.

Finally, we have shown the activity induced in Chronos-expressing interneurons by imaging under conditions similar to what is typically adopted for GCaMP 2P imaging (large FOV, fast and low-resolution scanning). Despite unfavorable temporal integration of photocurrents during scan illumination due to Chronos fast kinetics, in some cases, we recorded significant membrane depolarization. These results suggest that attention needs to be paid to the choice of the imaging conditions when coupling photostimulation and functional imaging investigations. Reduction of membrane depolarizations during functional imaging could be achieved by using more red-shifted calcium indicators with excitation that does not overlap with Chronos absorption spectrum. Further benefits could be obtained by minimizing the imaging beam dwell time on the cell using smart targeted-path scanning schemes (Lillis et al., 2008; Grewe et al., 2011) to define arbitrarily line-scan trajectories or scanless, camera-based detection (Quirin et al., 2014; Yang et al., 2015, 2016; Bovetti et al., 2017) able to minimize the imaging beam dwell time on the cell dendrites and axons.

Footnotes

  • This work was supported by the Agence Nationale de la Recherche (Grants ANR-10-INBS-0401 and ANR-14-CE13-0016), the National Institute of Health (Grant 1-U01-NS090501-01), the Getty Lab, the Medical Research Foundation (Grant DVS20131228920), the Federation pour la Recherche sur le Cervau (FRC), the Rotary Club through the program Espoir en Tete 2012, the Human Frontier Science Program (Grant RGP0015/2016), and a National Science Foundation International Postdoctoral Research Fellowship (A.J.F.). We thank Marta Gajowa and Fabio Simony-Conesa for help in mice viral injections; Christophe Tourain for technical support in building the holographic system; Vincent de Sars for software development; Cecile Jouffret for help in cell culture maintenance.

  • The authors declare no competing financial interests.

  • Correspondence should be addressed to Valentina Emiliani, Neurophotonics Laboratory, Wave Front Engineering Microscopy Group, CNRS UMR8250, Université Paris Descartes, 45 Rue des Saints Pères, 75270 Paris Cedex 06, France. valentina.emiliani{at}parisdescartes.fr

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The Journal of Neuroscience: 37 (44)
Journal of Neuroscience
Vol. 37, Issue 44
1 Nov 2017
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Submillisecond Optogenetic Control of Neuronal Firing with Two-Photon Holographic Photoactivation of Chronos
Emiliano Ronzitti, Rossella Conti, Valeria Zampini, Dimitrii Tanese, Amanda J. Foust, Nathan Klapoetke, Edward S. Boyden, Eirini Papagiakoumou, Valentina Emiliani
Journal of Neuroscience 1 November 2017, 37 (44) 10679-10689; DOI: 10.1523/JNEUROSCI.1246-17.2017

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Submillisecond Optogenetic Control of Neuronal Firing with Two-Photon Holographic Photoactivation of Chronos
Emiliano Ronzitti, Rossella Conti, Valeria Zampini, Dimitrii Tanese, Amanda J. Foust, Nathan Klapoetke, Edward S. Boyden, Eirini Papagiakoumou, Valentina Emiliani
Journal of Neuroscience 1 November 2017, 37 (44) 10679-10689; DOI: 10.1523/JNEUROSCI.1246-17.2017
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Keywords

  • holography
  • optogenetics
  • two-photon excitation

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