Figure 5. Epileptic phenotype of the kainate mouse model of TLE. A, Occurrence per minute of HPDs reminiscent of epileptic seizures in control animals (n = 23), 7 d after kainate injection (n = 12), 14 d after kainate injection (n = 12), and 28 d after kainate injection (n = 21). The increase is significant across the disease (control: median = 0, 25–75 percentile = 0–0, D7: 0, 0–0, D14: 0, 0–0, D28: 0.05, 0–0.26, **p < 0.01, ***p < 0.001). Although 1 animal presented a 0.14/min occurrence of seizures at D14, the 75th percentile remains equal to 0. B, E, Occurrence per minute of GS (B) and FRs (E) in the longitudinal paired group (n = 12 recordings at each time point for GS; n = 10 for FRs). FRs were not analyzed in 2 animals because of a small sampling rate. The increase is significant across the disease in GS (control: mean = 0, SEM = 0, D7: 0.39, 0.12, D14: 1.51, 0.34, D28: 1.59, 0.29) and FRs (control: median = 0.11, 25–75 percentile = 0–0.38, D7: 0.1, 0.04–0.29, D14: 0.62, 0.3–2, D28: 5.8, 3.58–12.54). C, F, Number of GSs (C) and FRs (F) in control preinjected animals (n = 23) and an unpaired group of 21 animals recorded at D28. The difference is statistically significant in GS (control: mean = 0, SEM = 0, D28: 1.68, 0.31) and FRs (control: median = 0.29, 25–75 percentile = 0.12–0.75, D28: 6.53, 3.65–17.39). D, G, Number of GS (D) and FRs (G) in control animals (n = 4) recorded 28 d after saline injection and an unpaired group of animals (n = 4) recorded 28 d after kainate injection. The difference is statistically significant in GSs (saline: mean = 0, SEM = 0, D28: 2.86, 0.98) and FRs (saline: 0.71, 25–75 percentile: 0.27–0.98, D28: 20.11, 11.65–58.28). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. For the statistical tests used, see Materials and Methods.