Figure 1-1
Anatomy of horizontal slices containing the presubiculum, including expression patterns of Channelrhodopsin and locations of biocytin filled recorded neurons (Extended data supporting Figure 1). A-D. The shape of presubiculum becomes increasingly broader from ventral (A) to dorsal (D) levels. Left column, horizontal DAPI (blue) stained sections, for dorso-ventral planes as indicated. A corresponds to the ventral presubiculum (close to DV -4 mm from Bregma), D corresponds to the dorsal part of the presubiculum (close to DV -2.8 mm from Bregma). Second from left, corresponding expression pattern of eYFP tagged Channelrhodopsin (green), for dorso-ventral levels A to D. Examples for individual slices containing biocytin filled presubicular neurons (white), matched to the dorso-central levels A to D. Two examples of recorded and biocytin filled neurons (MN903c1c2 and MNo09c1c2) have been reconstructed and are part of Figure 5. *indicates same slices within a row. DG, Dentate Gyrus; Sub, subiculum; PaS, parasubiculum; MEC, medial entorhinal cortex; ChR2, eYFP-tagged channelrhodopsin. Download Figure 1-1, TIF file