Figure 3. CRFR1cKO GrCs showing different electro-responsiveness from control mice. a, Examples of voltage responses elicited from −80 mV using step current injections in control for the floxed alleles (control flox), control for the Δα6- Cre (Control Cre), and CRFR1cKO mice. Instantaneous spike frequency is represented on top, showing rapid increase and marked adaptation in CRFR1cKO mice. b, Frequency/intensity plots for the same cells in a. Note that the CRFR1cKO GrC shows higher discharge frequency at low current injection and lower discharge frequency at high current injection compared with control and Cre cells, generating a characteristic bell-shaped response profile. The histograms at the bottom show average discharge frequency values at different current injection for all recorded cells (control flox n = 8, N = 4; control Cre n = 7, N = 4; CRFR1cKO n = 5, N = 3). c, Spike threshold, AHP, and spike peak in the different groups of GrC control flox n = 8, N = 4; control Cre n = 7, N = 4; CRFR1cKO n = 5, N = 3). CRFR1cKO GrCs spikes show higher threshold (control flox vs control Cre, p = 0.31; control flox vs CRFR1cKO, p = 0.0013; control Cre vs CRFR1cKO, p = 0.00015), smaller AHP (control flox vs control Cre, p = 0.73; control flox vs CRFR1cKO, p = 0.0037; control Cre vs CRFR1cKO, p = 0.0011), and smaller overshoot (control flox vs control Cre, p = 0.9; control flox vs CRFR1cKO, p = 0.052; control Cre vs CRFR1cKO, p = 0.029) compared with the 2 control groups. Data are represented as mean ± SEM. #p < 0.05, *p < 0.005 for one-way. Ind, Induction.