Article Figures & Data
Figures
- Figure 1.
Transduction pattern in two example cases. The sections presented here are representative of the sections used for each animal. A, DREADD transduction pattern shown in PFC of Case P. Regions within black squares are shown at higher magnification in C and D. B, DREADD transduction from PFC of Case Z. C, D, DREADD patches along principal sulcus from same slice as in A. Shown at 10× magnification. E, F, DREADD patches from same slice as in B. Shown at 10× magnification. Note DREADD-positive cells near principal sulcus (E) as well as orbital frontal cortex (F). Black arrowhead denotes presence of needle track. Scale bars: A and B, 2500 μm; C–F, 100 μm.
- Figure 2.
Spatial delayed response performance for vehicle and CNO sessions. A, Mean performance across all sessions for each case. B, Performance for each case broken down by delay (5, 10, 15, and 20 s). Case T had an additional trial time of 30 s.
- Figure 3.
Significant positive correlation between stereological and behavioral measures. Percentage of prefrontal neurons transduced with DREADD was significantly correlated with performance on the spatial delayed response task, calculated here as an aggregate deficit score from postoperative DREADD receptor activation (r = 0.8745, p = 0.0196).
Tables
- Table 1.
Surgery and injection schedule during the course of postoperative behavioral testing
Case Surgery date CNO (20 mg/kg) test session 1 2 3 4 A 12/11/13 11/04/14 11/25/14 B 12/10/13 11/04/14 11/07/14 11/25/14 12/10/14 P 04/09/15 05/19/15 05/21/15 07/21/15 T 08/02/16 10/06/16 11/06/16 12/13/16 Z 04/07/15 05/19/15 05/21/15 Case Vehicle: correct, % (SD) Pre-op/Post-op Drug (dose, mg/kg) Correct, % t statistic (p value) A 82.3 (13.3) Post-op CNO (10) 83.3 0.07 (0.47) Post-op CNO (20) 83.3 0.07 (0.47) Post-op CNO (20) 87.6 0.38 (0.35) B 86.4 (8.5) Post-op CNO (10) 83.3 −0.35 (0.37) Post-op CNO (20) 70.8 −1.79 (0.04) Post-op CNO (20) 54.2 −3.70 (0.001) Post-op CNO (20) 58.3 −3.22 (0.002) Post-op CNO (20) 87.5 0.13 (0.45) P 73.5 (7.8) Pre-op CNO (20) 70.8 −0.33 (0.37) Post-op CNO (20) 58.3 −1.87 (0.04) Post-op CNO (20) 58.3 −1.87 (0.04) Post-op CNO (10) 62.5 −1.36 (0.10) Post-op CNO (20) 70.8 −0.33 (0.37) Post-op CNO (20) 75.0 0.19 (0.43) T 86.1 (5.3) Pre-op C21 (20) 96.0 1.78 (0.05) Pre-op CNO (24.5) 92.0 1.066 (0.16) Post-op CNO (20) 93.3 1.30 (0.11) Post-op CNO (20) 56.7 −5.27 (<0.0005) Post-op CNO (20) 93.3 1.30 (0.11) Post-op C21 (20) 80.0 −1.09 (0.15) Post-op C21 (20) 76.7 −1.69 (0.06) Post-op C21 (20) 90.0 0.71 (0.25) Z 95.8 (5.6) Pre-op CNO (20) 83.3 −2.14 (0.03) Post-op CNO (20) 91.7 −0.71 (0.25) Post-op CNO (20) 100.0 0.72 (0.25) All sessions for each case are in chronological order. C21, Compound 21.
