Figure 7. Sex differences in the requirement for PKA in long term potentiation. A, Representative individual traces and time course of mean ± SEM normalized fEPSP slope for 1× HFS LTP experiments in males (n = 7 control, purple; n = 6 mPKI, green). Scale bars, 0.2 mV, 25 ms (also in B, D, and E). B, Representative individual traces and time course of mean ± SEM normalized fEPSP slope for all 1× HFS LTP experiments in females (n = 6 control, purple; n = 6 mPKI, green). C, Normalized increase in fEPSP slope for all 1× HFS LTP experiments where the magnitude of potentiation is shown separately for each control and mPKI experiment in males and females. PKA inhibition had no effect on 1× HFS LTP in males, but blocked 1× HFS LTP in females (Bonferroni post hoc test, ** indicates p < 0.01). D, Representative individual traces and time course of mean ± SEM normalized fEPSP slope for all 3× HFS LTP experiments in males (n = 6 control, purple; n = 6 mPKI, green). E, Representative individual traces and time course of mean ± SEM normalized fEPSP slope for all 3× HFS LTP experiments in females (n = 6 control, purple; n = 8 mPKI, green). F, Normalized increase in fEPSP slope for all 3× HFS LTP experiments in which the magnitude of potentiation is shown separately for each control and mPKI experiment in males and females. PKA inhibition had no effect on 3× HFS LTP in males, but attenuated 3× HFS LTP in females (Bonferroni post hoc test, *p < 0.05). G, Representative individual traces and time course of mean ± SEM normalized EPSC amplitude for all pairing-induced LTP experiments in males (n = 10 control, purple; n = 10 mPKI, green). Scale bars, 50 pA, 25 ms (also in H). H, Representative individual traces and time course of mean ± SEM normalized EPSC amplitude for all pairing-induced LTP experiments in females (n = 14 control, purple; n = 11 mPKI, green). I, Normalized increase in EPSC amplitude for all pairing induced LTP experiments in which the magnitude of potentiation is shown separately for each control and mPKI experiment in males and females. PKA inhibition attenuated pairing-induced LTP in males, but blocked pairing-induced LTP in females (*p < 0.05, ***p < 0.001, Bonferroni post hoc test).