Skip to main content

Main menu

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Collections
    • Podcast
  • ALERTS
  • FOR AUTHORS
    • Information for Authors
    • Fees
    • Journal Clubs
    • eLetters
    • Submit
  • EDITORIAL BOARD
  • ABOUT
    • Overview
    • Advertise
    • For the Media
    • Rights and Permissions
    • Privacy Policy
    • Feedback
  • SUBSCRIBE

User menu

  • Log out
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Neuroscience
  • Log out
  • Log in
  • My Cart
Journal of Neuroscience

Advanced Search

Submit a Manuscript
  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Collections
    • Podcast
  • ALERTS
  • FOR AUTHORS
    • Information for Authors
    • Fees
    • Journal Clubs
    • eLetters
    • Submit
  • EDITORIAL BOARD
  • ABOUT
    • Overview
    • Advertise
    • For the Media
    • Rights and Permissions
    • Privacy Policy
    • Feedback
  • SUBSCRIBE
PreviousNext
Articles

Demonstration of postsynaptic opioid modulation of thalamic projection neurons by the combined techniques of retrograde horseradish peroxidase and enkephalin immunocytochemistry

MA Ruda, J Coffield and R Dubner
Journal of Neuroscience 1 August 1984, 4 (8) 2117-2132; DOI: https://doi.org/10.1523/JNEUROSCI.04-08-02117.1984
MA Ruda
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
J Coffield
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
R Dubner
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Thalamic projection neurons represent a major source of nociceptive information from the dorsal horn to higher centers of the neuraxis. The synaptic relationship between thalamic projection neurons and the opioid peptide enkephalin (ENK) was examined at the light (LM) and ultrastructural (EM) level using the combined techniques of retrograde transport of horseradish peroxidase and ENK immunocytochemistry. Utilizing two different chromogens to develop the peroxidase reaction product, the two labeled neural elements could be readily distinguished at the LM level in the same tissue section. In the medullary, cervical, and lumbar levels of the dorsal horn of both the cat and monkey, at least 30% of the thalamic projection neurons in lamina I were observed at the LM level to be contacted by ENK-immunoreactive varicosities. In lamina V, approximately 50% of the thalamic projection neurons received ENK contacts. Since some neurons were not observed to receive a dense ENK innervation on their somata and proximal dendrites, these data suggest that there may be different functional types of thalamic projection neurons. At the EM level, the ENK-immunoreactive varicosities were observed to form asymmetrical synaptic contacts on the labeled somata and proximal dendrites of the projection neurons. In all cases, the ENK varicosities were morphologically similar and contained round or oval agranular vesicles and a few dense-core vesicles. These observations suggest that ENK acts to a substantial degree on postsynaptic opiate receptors located on thalamic projection neurons.

Back to top

In this issue

The Journal of Neuroscience: 4 (8)
Journal of Neuroscience
Vol. 4, Issue 8
1 Aug 1984
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
Email

Thank you for sharing this Journal of Neuroscience article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Demonstration of postsynaptic opioid modulation of thalamic projection neurons by the combined techniques of retrograde horseradish peroxidase and enkephalin immunocytochemistry
(Your Name) has forwarded a page to you from Journal of Neuroscience
(Your Name) thought you would be interested in this article in Journal of Neuroscience.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
View Full Page PDF
Citation Tools
Demonstration of postsynaptic opioid modulation of thalamic projection neurons by the combined techniques of retrograde horseradish peroxidase and enkephalin immunocytochemistry
MA Ruda, J Coffield, R Dubner
Journal of Neuroscience 1 August 1984, 4 (8) 2117-2132; DOI: 10.1523/JNEUROSCI.04-08-02117.1984

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Respond to this article
Request Permissions
Share
Demonstration of postsynaptic opioid modulation of thalamic projection neurons by the combined techniques of retrograde horseradish peroxidase and enkephalin immunocytochemistry
MA Ruda, J Coffield, R Dubner
Journal of Neuroscience 1 August 1984, 4 (8) 2117-2132; DOI: 10.1523/JNEUROSCI.04-08-02117.1984
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Responses to this article

Respond to this article

Jump to comment:

No eLetters have been published for this article.

Related Articles

Cited By...

More in this TOC Section

  • Choice Behavior Guided by Learned, But Not Innate, Taste Aversion Recruits the Orbitofrontal Cortex
  • Maturation of Spontaneous Firing Properties after Hearing Onset in Rat Auditory Nerve Fibers: Spontaneous Rates, Refractoriness, and Interfiber Correlations
  • Insulin Treatment Prevents Neuroinflammation and Neuronal Injury with Restored Neurobehavioral Function in Models of HIV/AIDS Neurodegeneration
Show more Articles
  • Home
  • Alerts
  • Visit Society for Neuroscience on Facebook
  • Follow Society for Neuroscience on Twitter
  • Follow Society for Neuroscience on LinkedIn
  • Visit Society for Neuroscience on Youtube
  • Follow our RSS feeds

Content

  • Early Release
  • Current Issue
  • Issue Archive
  • Collections

Information

  • For Authors
  • For Advertisers
  • For the Media
  • For Subscribers

About

  • About the Journal
  • Editorial Board
  • Privacy Policy
  • Contact
(JNeurosci logo)
(SfN logo)

Copyright © 2022 by the Society for Neuroscience.
JNeurosci Online ISSN: 1529-2401

The ideas and opinions expressed in JNeurosci do not necessarily reflect those of SfN or the JNeurosci Editorial Board. Publication of an advertisement or other product mention in JNeurosci should not be construed as an endorsement of the manufacturer’s claims. SfN does not assume any responsibility for any injury and/or damage to persons or property arising from or related to any use of any material contained in JNeurosci.