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Research Articles, Behavioral/Cognitive

Dopamine Receptor Dop1R2 Stabilizes Appetitive Olfactory Memory through the Raf/MAPK Pathway in Drosophila

Huan Sun, Tomoki Nishioka, Shun Hiramatsu, Shu Kondo, Mutsuki Amano, Kozo Kaibuchi, Toshiharu Ichinose and Hiromu Tanimoto
Journal of Neuroscience 1 April 2020, 40 (14) 2935-2942; DOI: https://doi.org/10.1523/JNEUROSCI.1572-19.2020
Huan Sun
1Graduate School of Life Sciences, Tohoku University, Aoba-Ku, Sendai 980-8577, Japan,
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Tomoki Nishioka
2Department of Cell Pharmacology, Graduate School of Medicine, Nagoya University, Nagoya 466-8550, Japan,
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  • ORCID record for Tomoki Nishioka
Shun Hiramatsu
1Graduate School of Life Sciences, Tohoku University, Aoba-Ku, Sendai 980-8577, Japan,
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Shu Kondo
3Invertebrate Genetics Laboratory, National Institute of Genetics, Mishima 411-8540, Japan,
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Mutsuki Amano
2Department of Cell Pharmacology, Graduate School of Medicine, Nagoya University, Nagoya 466-8550, Japan,
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Kozo Kaibuchi
2Department of Cell Pharmacology, Graduate School of Medicine, Nagoya University, Nagoya 466-8550, Japan,
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Toshiharu Ichinose
1Graduate School of Life Sciences, Tohoku University, Aoba-Ku, Sendai 980-8577, Japan,
4Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, Sendai 980-8578, Japan,
5Center for Transdisciplinary Research, Niigata University, Niigata 950-2181, Japan, and
6Department of Neuropharmacology, Nagoya City University, Nagoya 467-8603, Japan
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Hiromu Tanimoto
1Graduate School of Life Sciences, Tohoku University, Aoba-Ku, Sendai 980-8577, Japan,
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Abstract

In Drosophila, dopamine signaling to the mushroom body intrinsic neurons, Kenyon cells (KCs), is critical to stabilize olfactory memory. Little is known about the downstream intracellular molecular signaling underlying memory stabilization. Here we address this question in the context of sugar-rewarded olfactory long-term memory (LTM). We show that associative training increases the phosphorylation of MAPK in KCs, via Dop1R2 signaling. Consistently, the attenuation of Dop1R2, Raf, or MAPK expression in KCs selectively impairs LTM, but not short-term memory. Moreover, we show that the LTM deficit caused by the knockdown of Dop1R2 can be rescued by expressing active Raf in KCs. Thus, the Dop1R2/Raf/MAPK pathway is a pivotal downstream effector of dopamine signaling for stabilizing appetitive olfactory memory.

SIGNIFICANCE STATEMENT Dopaminergic input to the Kenyon cells (KCs) is pivotal to stabilize memory in Drosophila. This process is mediated by dopamine receptors like Dop1R2. Nevertheless, little is known for its underlying molecular mechanism. Here we show that the Raf/MAPK pathway is specifically engaged in appetitive long-term memory in KCs. With combined biochemical and behavioral experiments, we reveal that activation of the Raf/MAPK pathway is regulated through Dop1R2, shedding light on how dopamine modulates intracellular signaling for memory stabilization.

  • dopamine receptor
  • Drosophila
  • memory stabilization
  • Raf/MAPK pathway

Introduction

Dopamine signaling in the central nervous system is crucial for memory and behavioral adaptation. In addition to well described roles in conveying reinforcement signals and motivation (Busto et al., 2010; Puig et al., 2014), dopamine signaling has been shown to stabilize nascent memory traces. This role is shown to be conserved across animal phyla ranging from arthropoda to chordata (O'Carroll et al., 2006; Rossato et al., 2009; Plaçais et al., 2012; Musso et al., 2015; Takeuchi et al., 2016).

Studies using olfactory aversive memory of Drosophila melanogaster particularly provide solid evidence regarding how dopaminergic modulation stabilizes memory. Paired presentations of odor and electric shocks increase the activity of specific dopamine neurons (DANs) with oscillating intracellular calcium concentrations during consolidation (Plaçais et al., 2012). Consistently, the blockade of these DANs in the early retention period impedes long-term memory (LTM) consolidation, while activation of them facilitates it (Plaçais et al., 2012, 2017). Intriguingly, the same pair of DANs is also engaged in the consolidation of sugar-rewarded LTM with activity oscillation (Musso et al., 2015; Pavlowsky et al., 2018). Another class of DANs is required in the early consolidation phase for appetitive LTM as well (Ichinose et al., 2015). Similarly in courtship suppression learning, LTM consolidation is mediated by specific DANs (Krüttner et al., 2015). Notably, all of these DANs innervate a neural structure called the mushroom body (MB).

MB intrinsic neurons, Kenyon cells (KCs), express all four of the following dopamine receptors (Croset et al., 2018): Dop1R1 and Dop2R, the functional counterparts of mammalian dopamine D1 and D2 receptors, respectively (Sugamori et al., 1995; Hearn et al., 2002); and two other invertebrate-specific receptors, Dop1R2 and DopEcR (Mustard et al., 2005; Srivastava et al., 2005). Indeed, three of these receptors have been reported to function in KCs for consolidated memories, which are Dop1R1 (Qin et al., 2012; Ichinose et al., 2015; Krüttner et al., 2015), Dop1R2 (Musso et al., 2015; Plaçais et al., 2017), and Dop2R (Scholz-Kornehl and Schwärzel, 2016). Nevertheless, how dopamine regulates intracellular signaling in KCs to stabilize memory remains sparsely understood.

We here examined the role of different dopamine receptors in KCs for appetitive memory. Furthermore, with combined biochemical, genetic, and behavioral characterization, we found that Dop1R2 stabilizes appetitive memory through the Raf and mitogen-activated protein kinase (MAPK) pathway in KCs. As MAPK is a well characterized hub molecule to mediate synaptic plasticity, cytoskeleton reorganization, or transcriptional change, our work provides a critical link between the extracellular dopaminergic modulation and the intracellular signaling in the context of LTM processing.

Materials and Methods

Fly culture and strains.

Flies were cultured on the standard cornmeal medium at 24°C in a 12 h light/dark cycle. Canton-S was used as wild-type fly strain. MB010B-GAL4 (Aso et al., 2014) was obtained from Janelia Farm Research Campus. MBSW-GAL4 is the P{MB-switch}12–1 line (Mao et al., 2004). Fly strains obtained from the Bloomington Drosophila Stock Center include the following: UAS-IVS-mCD8::GFP (catalog #32189; Pfeiffer et al., 2010); GMR57C10-GAL4 (catalog #39171); GMR13F02-GAL4 (catalog #48571; Jenett et al., 2012); UAS-Dop1R1.RNAi (P{TRiP.HMC02344}; catalog #55239); UAS-Dop1R2.RNAi (P{TRiP.HMC02893}; catalog #51423); UAS-Dop2R.RNAi (P{TRiP.HMC02988}; catalog #50621); UAS-DopEcR.RNAi (P{TRiP.JF03415}; catalog #31981); UAS-Raf.RNAi [1] (P{TRiP.HMC04133}; catalog #55863); UAS-Raf.RNAi [2] (P{TRiP.HMC03854}; catalog #55679) and UAS-MAPK.RNAi (P{TRiP.HMS00173}; catalog #34855). UAS-Raf.GOF (catalog #106635; Brand and Perrimon, 1994) was acquired from the Kyoto Stock Center. Venus-tagged endogenous dopamine receptor strains were generated as described previously (Kondo et al., 2020).

Behavior assay.

Female and male flies, 5–9 d of age after eclosion, were subjected to behavioral assay. Flies were wet starved before and after conditioning so that the mortality reaches ∼10% at the test. Apparatus and protocol for the appetitive conditioning and test were described previously (Ichinose and Tanimoto, 2016). Unless specially stated, appetitive differential conditioning was performed, during which one odor [conditioned stimulus (CS+)] was reinforced by dried sucrose (Sigma-Aldrich) reward, while the other odor (CS−) was not (Figs. 1–5, 6A,B). Exposure of CS+ or CS− lasted for 1 min, with a 1 min interval in between. 4-Methylcyclohexanol (MCH; Sigma-Aldrich) and octan-3-ol (OCT; Merck), diluted in paraffin wax oil to 2% and 1.2%, respectively, were used as odors. At the test, flies were transferred into a T-maze, where two odors were sucked from the two ends. Flies were allowed to freely explore between two arms and were recorded by cameras (FFMV-03M2M and GS3-U3–51S5M, FLIR) for 2 min. The fly number in each arm was automatically counted using an ImageJ (National Institutes of Health) custom-made macro. Based on these numbers, the learning index (Tempel et al., 1983) was calculated for every second. An average of learning indices in the last 60 s, when the performance usually reaches the plateau (Ichinose and Tanimoto, 2016), was used as a single data point. As for single-odor learning (Fig. 6C–G), the protocol followed exactly that of differential learning, except that 1.2% OCT was replaced with the pure paraffin wax oil during both the conditioning and test phase. For unpaired stimuli presentation (Fig. 5), the sucrose reward was presented first for 1 min, followed by a rest interval of 2 min. Then 2% MCH and 1.2% OCT were presented, each for 1 min, with another 1 min interval in between.

Western blot analysis.

Starved flies were subjected to appetitive differential conditioning described above and were deep frozen by liquid nitrogen at the indicated time points afterward. The “naive” groups were similarly starved and mock trained without odors and sugar reward, and subsequently were frozen. Around 200 frozen flies were put into a tube and were vortexed to separate heads and bodies. The mixture was poured onto a prechilled metal mesh (710 μm aperture; TS-50–20, Tokyo Screen) to sieve the bodies, and subsequently onto another mesh (425 μm aperture; TS-50–20, Tokyo Screen) to sieve the heads. The frozen heads were ground to powder by using Multibeads shocker (Yasui Kikai) and were resuspended in lysis buffer (20 mm Tris/HCl, pH 7.4; 1 mm EDTA; 1 mm dithiothreitol; 150 mm NaCl; 1% igepal CA-630; PhosSTOP (Sigma-Aldrich); proteinase inhibitor cocktail (Sigma-Aldrich). The samples were sonicated and centrifuged at 20,000 × g for 20 min. Supernatants were collected and used as the protein extracts. Concentrations of the protein extracts were measured by BCA assay (Wako). For Western blot analysis, 15 μg of each sample was loaded to 10% acrylamide gel and subjected to SDS-PAGE. Separated proteins were transferred to PVDF membranes (Immobilon-FL, Millipore). The membranes were blocked with Blocking one-P (Nacalai Tesque) for 1 h and probed with primary antibodies [rabbit anti-phospho-p44/42 MAPK (catalog #4370, Cell Signaling Technology), or rabbit anti-p44/42 MAPK (catalog #4695, Cell Signaling Technology)]. Goat anti-rabbit Alexa Fluor 680 antibody (catalog #A20984, Thermo Fisher Scientific) was used as a secondary antibody. Bands of antibody binding were detected and quantified using Odyssey CLx imaging system (LI-COR).

Immunohistochemistry.

For Venus-tagged dopamine receptors and MAPK immunohistochemistry (Figs. 1, 3), brains were dissected in PBS, followed by the fixation in 4% paraformaldehyde in PBS for 2 h at room temperature. Brains were subsequently washed three times and incubated in blocking solution (PBS with 3% goat serum and 0.1% Triton X-100). Then brains were incubated with antibodies in PBS containing 1% goat serum and 0.1% Triton X-100. The following primary antibodies were used at the indicated dilution: rabbit anti-GFP (1:1000; catalog #A11122, Thermo Fisher Scientific), rabbit anti-p44/42 MAPK (1:500; catalog #4695, Cell Signaling Technology). Alexa Fluor 488 goat anti-rabbit (1:1000; catalog #A11034, Thermo Fisher Scientific) was used as the secondary antibody. As for phosphorylated MAPK (pMAPK) immunohistochemistry (Fig. 5), flies were instantly anesthetized on ice and brains were dissected in ice-cold fixative (PBS containing 4% paraformaldehyde and 15% saturated picric acid solution). Then brains were fixed in the preceding fixative for 2 h on ice. Washing and blocking were performed similarly as stated above, but on ice instead. Rabbit anti-phospho-p44/42 MAPK (1:500; catalog #4370, Cell Signaling Technology) and Alexa Fluor 568 goat anti-rabbit (1:1000; catalog #A11036, Thermo Fisher Scientific) were used as primary and secondary antibodies, respectively, at the indicated dilution. Images were obtained using an Olympus FV1200 confocal microscope, acquired at the same time periods under the identical microscope setting. For counting pMAPK-positive cells, the experimenter was blinded regarding the sample identity: samples were randomized by another experimenter so that they could not be identified by the one who counted the cells. The average number from the two brain hemispheres was used as a single data point (Fig. 5B).

Drug administration.

RU486 (mifepristone; Sigma-Aldrich) was administrated with food for 2 d, then with water during food deprivation. RU486 was removed for the last 2 h before conditioning and after conditioning to avoid any nonspecific effects (Mao et al., 2004). RU486 was dissolved in ethanol (10 mg/ml) and mixed with melted food or water in a final concentration of 200 μm (Mao et al., 2004). The same amount of ethanol was added to the food or water for the control groups.

Data analysis and statistics.

Statistical analyses were performed on GraphPad Prism 6 (GraphPad Software). Data were analyzed with parametric statistics: one-sample t test or one-way ANOVA followed by Sidak's multiple-comparisons test, when the assumption of normal distribution (Shapiro–Wilk normality test) and homogeneity of variance (Bartlett's test) were not violated. Otherwise, nonparametric statistics, Kruskal–Wallis test followed by Dunn's multiple-comparisons test, were performed. The significance level of statistical tests was set to 0.05.

Results

Dopamine receptors in the MB are differently engaged in short-term and long-term appetitive memory

To examine the role of the dopamine receptors for appetitive memory retention, we systematically characterized the requirement of them from short-term memory (STM) to LTM. To this end, we knocked down each of them in KCs by transgenic RNAi, using a KC-specific split-GAL4 strain MB010B-GAL4 that labels α/β, α′/β′ and γ KCs (Vogt et al., 2014). The transgenic RNAi effectively reduced the level of Dop1R1, Dop1R2, and Dop2R in the MB, visualized by the endogenous receptors tagged with the Venus yellow fluorescent protein (Fig. 1A; Kondo et al., 2020). Because the RNAi against DopEcR did not show a strong reduction (data not shown), we excluded the receptor from the following behavioral analysis. Then we tested the sugar-rewarded memory at 5 min (STM), 3 h [often referred to as middle-term memory (MTM)], and 24 h (LTM; Krashes and Waddell, 2008; Colomb et al., 2009) after conditioning (Fig. 1B). Attenuation of Dop1R1 expression in KCs severely impaired appetitive memory regardless of the retention time tested here. In contrast, attenuation of Dop1R2 expression in KCs left STM and MTM intact but impaired LTM, consistent with previous observations (Musso et al., 2015). We did not observe significant differences when Dop2R expression was attenuated. These results suggest distinct roles of dopamine receptors Dop1R1 and Dop1R2 for mediating dopaminergic appetitive reinforcement and stabilizing memory, respectively.

Figure 1.
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Figure 1.

Differential engagement of dopamine receptors in short-term and long-term olfactory appetitive memory. A, Verification of the transgenic RNAi. Each dopamine receptor is knocked down in Kenyon cells and the endogenous protein is visualized by tagging the Venus yellow fluorescent protein. The Venus protein is stained using antibodies described in Materials and Methods. Genotypes in the control and the knockdown groups are as follows: (top) Dop1R1-Venus,R13F02-GAL4/+ and Dop1R1-Venus,R13F02-GAL4/UAS-Dop1R1.RNAi; (middle) Dop1R2-Venus, R13F02-GAL4/+, and Dop1R2-Venus, R13F02-GAL4/UAS-Dop1R2.RNAi; (bottom) Dop2R-Venus/+;;R13F02-GAL4/+ vs Dop2R-Venus/+;; R13F02-GAL4/UAS-Dop2R.RNAi. R13F02-GAL4 labels α/β, α′/β′ and γ KCs. Z-projection images containing the horizontal and vertical lobes are shown. Scale bars, 20 μm. B, Each of the three dopamine receptors is knocked down in KCs, and appetitive memory is measured at different retention times. Attenuation of Dop1R1 expression severely impairs 5 min, 3 h, or 24 h appetitive memory (5 min: F(6,51) = 8.17, p < 0.0001; 3 h: H = 24.34, p = 0.0005, df = 6; 24 h: F(6,65) = 12.14, p < 0.0001; MB010B-GAL4/UAS-Dop1R1.RNAi vs GAL4 and UAS controls: 5 min: p < 0.0001 to both; 3 h: p = 0.0018 and p = 0.0002, respectively; 24 h: p < 0.0001 to both). Attenuation of Dop1R2 expression impairs 24 h memory but leaves 5 min and 3 h memory intact (MB010B-GAL4/UAS-Dop1R2.RNAi vs controls: 5 min: p = 0.073 and p = 0.1726, respectively; 3 h: p > 0.9 to both; 24 h: p = 0.0174 and p = 0.0072, respectively). Memory disturbance in Dop2R expression-attenuated flies is not detected (MB010B-GAL4/UAS-Dop2R.RNAi vs controls: 5 min: p > 0.9 to both; 3 h: p > 0.9 to both; 24 h: p > 0.9 and p = 0.588, respectively). n = 8–12. Bars and error bars, mean ± SEM, respectively. *p < 0.05, ***p < 0.001. ns, Not significant (p > 0.05).

The Raf/MAPK pathway stabilizes appetitive memory in KCs

To probe into intracellular signaling under these dopamine receptors, we referred to the results of our previous phosphoproteomic analysis in the mouse striatum (Nagai et al., 2016a,b). This analysis identified many upregulated or downregulated phosphorylation sites in response to the application of dopamine receptor agonists (Nagai et al., 2016a,b). The emergence of MAPK and numerous putative MAPK phosphorylation target proteins, along with Raf (MAPKKK) inspired us to hypothesize that the Raf/MAPK pathway as an effector of dopamine signaling.

To investigate the role of Raf in appetitive memory maintenance, we downregulated Raf expression in KCs and tested memory retention. Attenuation of Raf expression using two independent RNAi insertions caused a selective deficit in 24 h memory, while leaving 5 min and 3 h memories intact (Fig. 2A), suggesting the selective role of Raf in LTM. This retention time course was similar to that of the knockdown of Dop1R2 in KCs (Fig. 1B).

Figure 2.
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Figure 2.

Raf is specifically required for appetitive long-term memory. A, Attenuation of Raf expression in KCs impairs 24 h, but not 5 min or 3 h memory (24 h: F(4,71) = 6.581, p = 0.0001; MB010B-GAL4/UAS-Raf.RNAi[1] vs GAL4 and UAS controls: p = 0.0013 and p = 0.0062, respectively; MB010B-GAL4/UAS-Raf.RNAi[2] vs controls: p = 0.0031 and p = 0.0239, respectively, n = 11–17; 5 min: F(4,44) = 0.3068, p = 0.8719; 3 h: F(4,45) = 1.091, p = 0.3723, n = 9–12). B, C, Knockdown of Raf in adult KCs using RU486-induced MBSW-GAL4 exhibits intact 5 min memory (B: F(4,35) = 0.919, p = 0.4640, n = 8), while showing 24 h memory defect (C: F(4,53) = 6.173, p = 0.0004; MBSW-GAL4/UAS-Raf.RNAi[1] vs controls: p = 0.0191 and p = 0.0150, respectively; MBSW-GAL4/UAS-Raf.RNAi[2] vs controls: p = 0.0113 and p = 0.0033, respectively, n = 9–14). D, Control flies without RU486 feeding attain intact 24 h memory (F(4,47) = 0.01589, p > 0.9, n = 9–11). Bars and error bars, mean ± SEM, respectively. *p < 0.05, **p < 0.01. ns, Not significant (p > 0.05).

The evolutionary conserved oncogene Raf acts as a core component in multiple cellular processes during development (Leicht et al., 2007). To address the role of Raf in adult but not in developing KCs, we used the RU486-inducible transgenic expression in the MB (MBSW-GAL4) to restrict the Raf knockdown spatially and temporally (Roman et al., 2001; Mao et al., 2004). The adult-specific Raf attenuation impaired 24 h memory (Fig. 2C), in contrast to the intact STM (Fig. 2B) or LTM in the control group without RU486 application (Fig. 2D).

Next, we questioned whether the downstream effector MAPK is also required for LTM. Consistent with the selective LTM impairment of the Raf knockdown, attenuating the expression of the rolled (rl) gene encoding MAPK in adult KCs significantly impaired 24 h memory, without perturbing 5 min memory (Fig. 3A–C). Immunohistochemistry of MAPK confirmed the reduction of the protein in the MB (Fig. 3D). Collectively, these results reveal that the Raf/MAPK pathway is required in KCs for appetitive memory stabilization.

Figure 3.
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Figure 3.

MAPK is specifically required for appetitive long-term memory. A, B, Knockdown of MAPK in adult KCs leaves 5 min memory intact (A: F(2,21) = 0.8162, p = 0.4556, n = 8), while impairs 24 h memory (B: F(2,42) = 11.39, p = 0.0001, n = 15). C, Flies without RU486 feeding show intact 24 h memory (F(2,21) = 0.019, p > 0.9, n = 8). D, MAPK expression is suppressed in the MB when the RNAi is induced in adult KCs (right, RU486+), compared with the control (left, RU486−). Arrowheads point to the β lobes. Scale bars, 20 μm. Bars and error bars, mean ± SEM, respectively. ***p < 0.001. ns, Not significant (p > 0.05).

Appetitive learning induces Dop1R2-dependent MAPK activation

The selective requirement of Dop1R2, Raf, and MAPK for appetitive LTM prompted us to hypothesize that the Raf/MAPK pathway is activated under the control of Dop1R2 upon appetitive learning. Electric shock conditioning was reported to induce MAPK diphosphorylation, thereby activating the kinase (Moressis et al., 2009; Pagani et al., 2009; Miyashita et al., 2018; Zhang et al., 2018). To examine whether appetitive training induces MAPK activation, we monitored the time course of pMAPK by sampling fly heads in different time points after appetitive conditioning (Fig. 4A). Quantification of the pMAPK ratio to the total MAPK (tMAPK) revealed significant increases immediately after learning (i.e., 1, 3, and 10 min), which returned to the basal level after 30 min. Interestingly, this experience-dependent increase of MAPK phosphorylation disappeared by neuronal knockdown of Dop1R2 (Fig. 4B).

Figure 4.
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Figure 4.

Experience-induced MAPK phosphorylation requires Dop1R2. A, Phosphorylation of MAPK before and after appetitive conditioning in wild-type flies. Representative Western blot shows pMAPK and tMAPK in naive (N) and conditioned flies at different time points (from 1 min to 3 h) after training. Ratio of pMAPK to tMAPK is plotted in the bar graph. pMAPK ratio elevates within the first 10 min, then returns to basal level after 30 min (1 min: t(16) = 2.859, p = 0.0114; 3 min: t(12.97) = 2.638, p = 0.0205; 10 min: t(12.81) = 2.628, p = 0.0211; 30 min: t(14.71) = 0.3083, p = 0.7622; 1 h: t(15.59) = 0.2725, p = 0.7888; 3 h: t(15.98) = 0.4946, p = 0.6276, n = 8–9). B, The experience-dependent MAPK activation disappears in Dop1R2-attenuated flies. Western blot shows pMAPK and tMAPK in naive (N) and conditioned (3 min and 10 min after conditioning) transgenetic flies. Ratio of pMAPK to tMAPK is shown. pMAPK ratio relatively increases after conditioning in genetic control flies, but not in the Dop1R2-attenuated flies (R57C10-GAL4/+: F(2,21) = 6.438, p = 0.0066; +/UAS-Dop1R2.RNAi: F(2,21) = 9.013, p = 0.0015; R57C10-GAL4/UAS-Dop1R2.RNAi: F(2,21) = 0.007, p > 0.9, n = 8). Bars and error bars, mean ± SEM, respectively. Each bar representing conditioned flies are compared with the corresponding naive controls. *p < 0.05, **p < 0.01. ns, Not significant (p > 0.05).

To monitor learning-dependent MAPK activation in KCs, we next performed immunohistochemistry of pMAPK. By counting the number of pMAPK-positive KCs, we found that the paired presentation of sugar reward and an odor induces MAPK phosphorylation in KCs, compared with the unpaired group (Fig. 5A,B). Strikingly, associative training failed to induce MAPK phosphorylation in KCs where Dop1R2 expression is downregulated (Fig. 5A,B). These results revealed that the association of sugar reward and an odor activates MAPK in KCs, in a Dop1R2-dependent manner.

Figure 5.
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Figure 5.

Paired presentation of sugar reward and an odor induces Dop1R2-dependent MAPK phosphorylation in KCs. A, pMAPK immunohistochemistry at the KC soma region (outlined) is shown in naive (left), 3 min after the unpaired presentation of sugar reward and an odor (middle) or 3 min after the paired presentation (right). KCs are labeled by mCD8::GFP and Dop1R2 is knocked down in KCs (Control in top, UAS-mCD8::GFP/+;MB010B-GAL4/+; Dop1R2 knockdown in bottom, UAS-mCD8::GFP/+;MB010B-GAL4/UAS-Dop1R2.RNAi). B, The number of pMAPK-positive KCs (pMAPK counts) increases in a coincidental and Dop1R2-dependent manner. Unpaired presentation of sugar reward and an odor increases pMAPK counts compared with the naive in both control (F(5,42) = 47.2, p < 0.0001; naive vs unpaired: p < 0.0001), and the Dop1R2 knockdown flies (naive vs unpaired: p < 0.0001). A further increase of pMAPK in the paired group is observed in control flies (paired vs unpaired: p = 0.0009), but not in Dop1R2 knockdown flies (paired vs unpaired: p = 0.3865). A significant difference in pMAPK counts between the control and the Dop1R2 knockdown flies is detected after paired presentation (control vs Dop1R2 knockdown: p < 0.0001). n = 7–9. pMAPK counts per hemispheres is shown. The sample images with median pMAPK counts in each group are selected for A. Bars and error bars, mean ± SEM, respectively. ***p < 0.001. ns, Not significant (p > 0.05).

Expression of constitutively active Raf rescues the LTM deficit caused by the Dop1R2 knockdown

If the Raf/MAPK pathway is the intracellular target of Dop1R2 signaling for appetitive LTM, activation of Raf could surrogate Dop1R2 signaling. To examine this hypothesis, we transiently expressed a truncated form of Raf lacking the regulatory domain (Raf.GOF; Brand and Perrimon, 1994) in KCs together with attenuating Dop1R2-expression. Intriguingly, the 24 h memory deficit in Dop1R2 knockdown flies was rescued by overexpressing Raf.GOF (Fig. 6A; MBSW-GAL4, UAS-Raf.GOF/UAS-Dop1R2.RNA). In contrast, the impairment due to the Dop1R1 knockdown was not rescued by Raf.GOF expression (Fig. 6A; MBSW-GAL4, UAS-Raf.GOF/UAS-Dop1R1.RNA). Together, these observations reveal that Raf/MAPK is a pivotal downstream effector of Dop1R2, but not Dop1R1, during appetitive memory stabilization.

Figure 6.
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Figure 6.

Expression of constitutively active Raf rescues the LTM deficit caused by the Dop1R2 knockdown. A, Attenuation of Dop1R2 or Dop1R1 expression, as well as the overexpression of active Raf (Raf.GOF) in adult KCs impairs 24 h memory (F(8,125) = 14.68, p < 0.0001; MBSW-GAL4/UAS-Dop1R2.RNAi vs GAL4 and UAS controls: p = 0.0011 and p = 0.0083, respectively; MBSW-GAL4/UAS-Dop1R1.RNAi vs controls, p < 0.0001 to both; MBSW-GAL4/UAS-Raf.GOF vs controls: p = 0.0004 and p = 0.0034). The deficit in 24 h memory caused by Dop1R2 attenuation is rescued by the overexpression of Raf.GOF, whereas that by Dop1R1 attenuation is not (MBSW-GAL4, UAS-Raf.GOF/UAS-Dop1R2.RNAi vs MBSW-GAL4/UAS-Dop1R2.RNAi: p = 0.0275; MBSW-GAL4, Raf.GOF/Dop1R1.RNAi vs MBSW-GAL4/Dop1R1.RNAi: p > 0.9). n = 12–17. B, Overexpression of Raf.GOF does not alter 5 min memory (F(2,27) = 0.3734, p = 0.6919, n = 9–11). C, Schematics of single-odor learning by using only MCH. (Note that all preceding figures show the results of differential learning using two odors, MCH and octan-3-ol.) D–F, In single-odor learning, the overexpression of Raf.GOF does not impair 24 h memory (D: F(2,32) = 0.8472, p = 0.4380, n = 13–14), while knockdown of Raf or Dop1R2 does (E: F(4,36) = 6.789, p = 0.0004; MBSW-GAL4/UAS-Raf.RNAi[1] vs controls: p = 0.0008 and p = 0.008, respectively; MBSW-GAL4/UAS-Raf.RNAi[2] vs controls: p = 0.0140 and p = 0.0311, respectively; F: p = 0.0062, n = 8–12). G, In single-odor learning, the overexpression of Raf.GOF rescues the impaired 24 h memory caused by Dop1R2-attenuation in KCs (F(5,8) = 7.077, p < 0.0001; MBSW-GAL4, UAS-Raf.GOF/UAS-Dop1R2.RNAi vs MBSW-GAL4/UAS-Dop1R2.RNAi: p = 0.0222; MBSW-GAL4/UAS-Raf.GOF vs controls: p > 0.9 and p = 0.4995, respectively; MBSW-GAL4/UAS-Dop1R2.RNAi vs controls: p = 0.0004 and p = 0.0011, respectively, n = 8–11). Bars and error bars, mean ± SEM, respectively. *p < 0.05, **p < 0.01, ***p < 0.001. ns, Not significant (p > 0.05).

In the course of the experiments, we noticed that overexpression of Raf.GOF alone reduced LTM but not STM (Fig. 6A,B), a similar memory impairment to the knockdown of Raf (Fig. 2). In the previous experiments, we used the standard differential conditioning protocol, in which one of the two odors presented during conditioning was not paired with the sugar reward, and therefore serves as the reference odor (Tempel et al., 1983). As the presentation of an unpaired odor during training has been shown to be critical for odor discrimination (Barth et al., 2014; Schleyer et al., 2018), we simplified the paradigm by presenting only one odor in training and test, referred to as single-odor learning here (Fig. 6C). Interestingly, overexpression of Raf.GOF in KCs did not lead to 24 h memory deficit in the protocol without a reference odor, while knockdown of Raf or Dop1R2 did as in differential learning (Fig. 6D–F). Strikingly, expression of Raf.GOF in KCs significantly rescued the memory impairment of transient Dop1R2 knockdown in single-odor conditioning as well, suggesting that activation of Dop1R2 and Raf is critical for associative reward memory (Fig. 6G). A previous report showed that the expression of Raf.GOF does not significantly alter the basal pMAPK level (Zhang et al., 2018), but we observed a behavioral defect in the differential conditioning (Fig. 6A). These results imply that the titration of the Raf activity is critical for processes relevant to an unpaired reference odor.

Discussion

Our study supports the idea that Dop1R2 signaling through the Raf/MAPK pathway in KCs is critical in stabilizing appetitive memory. This could be achieved through acquisition or consolidation of appetitive LTM. How is post-training Dop1R2 signaling triggered in this context? Accumulating evidence implies that Dop1R2 detects the basal dopamine release after learning (Berry et al., 2012; Musso et al., 2015; Ichinose et al., 2017). In aversive olfactory learning, the post-training enhancement of the oscillatory activity of MB-projecting DANs (MB-MP1 and MB-MV1) underlies LTM consolidation (Plaçais et al., 2012), and Dop1R2 in KCs is responsible for detecting the enhanced dopamine signals (Plaçais et al., 2017). This signaling is also reported to mediate forgetting early labile memory (Berry et al., 2012), suggesting distinct neural mechanisms to regulate memories with different temporal dynamics. In appetitive learning, Dop1R2 is suggested to be the mediator of the oscillating DANs, which represent the energy value of the reward and consolidate LTM (Musso et al., 2015; Pavlowsky et al., 2018). Collectively, after conditioning Dop1R2 signaling upon specific reinforcement input is a conserved mechanism to stabilize LTM. As MB-projecting DANs are also engaged in conveying reward information during memory acquisition (Burke et al., 2012; Liu et al., 2012; Yamagata et al., 2015), the Dop1R2/Raf/MAPK pathway might additionally be involved during the acquisition of LTM.

In contrast to the well characterized receptor tyrosine kinase signaling, it is rather unexpected to find the Raf/MAPK pathway as a downstream target of Dop1R2, a G-protein-coupled receptor (Figs. 5, 6). Dop1R2 was recently shown to have a preferential affinity to the Gαq subunit to elicit a robust intracellular Ca2+ increase upon ligand stimulation in KCs (Himmelreich et al., 2017; Handler et al., 2019). There are multiple lines of biochemical evidence suggesting that Gαq-dependent Ca2+ signals could trigger several pathways, such as small GTPase Rap1, protein kinase C, or Ras, to activate Raf (Liebmann, 2001; Goldsmith and Dhanasekaran, 2007). Furthermore, some reports suggested that calcium influx through N-methyl-d-aspartate receptor induces transient MAPK phosphorylation (English and Sweatt, 1996; Atkins et al., 1998). Hence, intracellular Ca2+ might be the key second-messenger system to link Dop1R2 and Raf/MAPK in appetitive LTM.

We found that MAPK has a pivotal role to stabilize appetitive memory in KCs (Fig. 3). MAPK signaling is known to regulate different cellular processes ranging from cytoskeletal dynamics to transcriptional modulation (Pullikuth and Catling, 2007; Giese and Mizuno, 2013). In Drosophila, a recent work unveiled that MAPK stabilizes presynaptic structural changes in KCs upon associative training with electric shocks, reportedly by changing the activity of an actin cytoskeleton regulator (Zhang et al., 2018). Such MAPK-induced cytoskeletal change might also occur in appetitive learning. Alternatively, a recent study showed that LTM consolidation involves MAPK translocation to the nuclei in KCs (Li et al., 2016). Consistently, it is reported that MAPK activates transcription factors like c-Fos and cAMP response element-binding protein (CREB) in KCs to form aversive LTM (Miyashita et al., 2018). Appetitive LTM is also dependent on CREB in KCs (Krashes and Waddell, 2008; Widmer et al., 2018). Collectively, we propose that MAPK stabilizes appetitive memory by regulating these transcription factors. Future investigation on the downstream of the MAPK pathway should reveal the newly transcribed genes for memory stabilization.

Footnotes

  • This study was supported by Ministry of Education, Culture, Sports, Science and Technology–Japan/JSPS KAKENHI (17H05545, 17H01378, 15K14307 to H.T.; 16K20919 to T.I.; 17H01380 to K.K.) and Strategic Research Program for Brain Sciences (H.T., K.K., M.A.). The Otsuka Toshimi Scholarship Foundation provided financial support during the study (to H.S.). We thank Nobuhiro Yamagata, Kei Ito, and Ayako Abe for technical and/or instructional supports at inception stage. We also thank Tomoyuki Miyashita, Yukinori Hirano, Bruce A. Edgar, and Minoru Saitoe for technical assistance and/or fly sharing, some of which were not shown in this article. In addition, we thank the Janelia FlyLight Project Team, the Bloomington Drosophila Stock Center, and the Kyoto Stock Center for fly strain provision.

  • The authors declare no competing financial interests.

  • Correspondence should be addressed to Hiromu Tanimoto at hiromut{at}m.tohoku.ac.jp or Toshiharu Ichinose at toshiharu.ichinose.c1{at}tohoku.ac.jp

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The Journal of Neuroscience: 40 (14)
Journal of Neuroscience
Vol. 40, Issue 14
1 Apr 2020
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Dopamine Receptor Dop1R2 Stabilizes Appetitive Olfactory Memory through the Raf/MAPK Pathway in Drosophila
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Dopamine Receptor Dop1R2 Stabilizes Appetitive Olfactory Memory through the Raf/MAPK Pathway in Drosophila
Huan Sun, Tomoki Nishioka, Shun Hiramatsu, Shu Kondo, Mutsuki Amano, Kozo Kaibuchi, Toshiharu Ichinose, Hiromu Tanimoto
Journal of Neuroscience 1 April 2020, 40 (14) 2935-2942; DOI: 10.1523/JNEUROSCI.1572-19.2020

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Dopamine Receptor Dop1R2 Stabilizes Appetitive Olfactory Memory through the Raf/MAPK Pathway in Drosophila
Huan Sun, Tomoki Nishioka, Shun Hiramatsu, Shu Kondo, Mutsuki Amano, Kozo Kaibuchi, Toshiharu Ichinose, Hiromu Tanimoto
Journal of Neuroscience 1 April 2020, 40 (14) 2935-2942; DOI: 10.1523/JNEUROSCI.1572-19.2020
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Keywords

  • dopamine receptor
  • Drosophila
  • memory stabilization
  • Raf/MAPK pathway

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