Figure 8. Physiological assessment of MF boutons. A, Example two-photon image of a cerebellar GC from a Pclogt/gt rat filled with ATTO dye. B, Average data of biophysical properties of GCs for Pclowt/wt and Pclogt/gt rats. The input resistance of GCs was higher in Pclogt/gt compared with Pclowt/wt (Pclowt/wt = 722.6 ± 76.24 mΩ, n = 22 cells; Pclogt/gt = 1160 ± 154.9 mΩ, n = 23 cells; n = 3 rats per genotype; Mann–Whitney U test, U = 165, *p = 0.0462). Whereas no differences were found in capacitance (Pclowt/wt = 3.717 ± 0.269 pF, n = 21 cells; Pclogt/gt = 3.643 ± 0.231 pF, n = 23 cells; n = 3 rats per genotype; Mann–Whitney U test, U = 236.5, p = 0.912), resting membrane potential (Vm) (Pclowt/wt = −99.96 ± 1.261 mV, n = 18 cells; Pclogt/gt = −100.8 ± 0.786 mV, n = 23 cells; n = 3 rats per genotype; Mann–Whitney U test, U = 187, p = 0.612), the half-duration of the AP (Pclowt/wt = 179.7 ± 11.84 μs, n = 18 cells; Pclogt/gt = 172.2 ± 7.366 μs, n = 23 cells; n = 3 rats per genotype; Mann–Whitney U test, U = 200, p = 0.866), the amplitude of the AP (Pclowt/wt = 67.85 ± 3.016 mV, n = 18 cells; Pclogt/gt = 63.92 ± 2.761 mV, n = 23 cells; n = 3 rats per genotype; Mann–Whitney U test, U = 166, p = 0.291), and the voltage threshold to elicit an AP (Pclowt/wt = −51.27 ± 1.748 mV, n = 18 cells; Pclogt/gt = −48.6 ± 1.659 mV, n = 23 cells; n = 3 rats per genotype; Mann–Whitney U test, U = 153 p = 0.162). C, mEPSCs from Pclogt/gt GCs were not different in their amplitude (Pclowt/wt = −19.62 ± 1.682 pA, n = 15 cells from 2 rats; Pclogt/gt = −22.44 ± 1.765 pA, n = 23 cells from 3 rats; Mann–Whitney U test, U = 136, p = 0.286) but in their frequency (Pclowt/wt = 0.102 ± 0.0167 Hz, n = 15 cells; Pclogt/gt = 0.257 ± 0.0481 Hz, n = 22 cells; n = 3 rats per genotype; Mann–Whitney U test, U = 98.5, *p = 0.0392). D, EPSCs from GCs measured after stimulation of single MFs were increased in Pclogt/gt compared with Pclowt/wt (Pclowt/wt = 47.58 ± 12.12 pA, n = 13 cells; Pclogt/gt = 67.62 ± 9.64 pA, n = 15 cells; n = 3 rats per genotype; Mann–Whitney U test, U = 52, *p = 0.0356), whereas the decay of the EPSCs was not altered (Pclowt/wt = 1.79 ± 0.258 ms, n = 12 cells; Pclogt/gt = 1.404 ± 0.141 ms, n = 14 cells; n = 3 rats per genotype; Mann–Whitney U test, U = 60, *p = 0.231). Right, Example traces of evoked EPSCs, as quantified in D, in response 1 Hz stimulation in the presence of 20 μm SR95531 and 40 μm d-APV. Scale bar: A, 20 μm. Error bars indicate SEM. Data points represent individual cells from 3 rats per genotype. Animals used in this experiment are as follows: wt18-wt20; ko17-ko19. ns, not significant.