Figure 6-1
Effect of TLQP21 on microglial migration. A) Boyden chamber assay: cells crossing the polycarbonate membrane (8 µm pore size) into the TLQP21-containing lower chamber were stained by Diff-quick and were displayed in light microscopic images, later processed with ImageJ for counting. TLQP21 was applied at the concentrations indicated, either with without (no) gradient as indicated. A scrambled TLQP21 peptide was also applied using a gradient. No gradient means that TLQP21 was present at the same concentration in the upper and lower chambers. B) Scratch assay: the scratch was implemented on a layer of microglial cells, and cells migrating into the initial scratched area were analyzed after an incubation for 3 h (n = 9/group). TLQP21 was applied at the indicated concentrations. Download Figure 6-1, TIF file