Figure 1. mNAc D1 neurons project throughout the rostrocaudal extent of the VTA. A, Schematic showing injection of AAV2-EF1a-DIO-Syb2-EGFP into the NAc shell and medial core of D1-Cre animals. B, Fluorescent image showing Syb2-eGFP expression (green, 488 nm) in the NAc of a D1-Cre mouse. C, D, Fluorescence image showing Syb2-eGFP (green, 488 nm) in the ventral portion of the VTA, throughout rostrocaudal extent, adjacent to TH+ neurons (blue, 405 nm). Region of high magnification in E, F highlighted (dotted line box). E, Fluorescent image showing Syb2-eGFP expression (green, 488 nm) in the NAc of a D2-Cre mouse. F, Fluorescence image without visible expression of Syb2-eGFP in the VTA (green, 488 nm). G, H, High-magnification confocal image showing Syb2-eGFP (green, 488 nm) expression interspersed among TH+ cells (blue, 405 nm). Shown without (G) and with 405 channel imaging (I). LV, Lateral ventricle; ac, anterior commissure; LS, lateral septum. I, J, High-magnification confocal image showing minimal Syb2-eGFP in the VTA (green, 488 nm) expression interspersed among TH+ cells (blue, 405 nm). Shown without (I) and with 405 channel imaging (J).