The Wiring Logic of an Identified Serotonergic Neuron That Spans Sensory Networks

This work was supported by National Institutes of Health DC 016293 and USAFOSR FA9550-17-1-0117 to A.M.D.; Howard Hughes Medical Institute Janelia Research Campus Visiting Scientist Program project to A.M.D. and K.E.C.; and Wellcome Trust Collaborative Award 203261/Z/16/Z and National Institutes of Health RF1 MH120679 01 Award to D.D.B. The Howard Hughes Medical Institute supported the generation and hosting of the FAFB dataset. We thank the FAFB tracing community for helpful insights and contributions of neuron tracings; Tom Kazimiers and Andrew Champion for CATMAID development; Peter Li for development of the autosegmented instance of the FAFB dataset; Eric Perlman for making the autosegmented dataset available in CATMAID; the Cambridge Drosophila Connectomics group, especially Greg Jefferis, Marta Costa, Philipp Schlegel, Alex Bates, Ruairi Roberts, Robert Turnbull, Lisa Marin, and Nik Drummond for use of hundreds of neuron reconstructions, assistance with custom analyses, and facilitating access to the autosegmented FAFB dataset; Feng Li, Mert Erginkaya, Johann Schor, Jeremy Johnson, Shrey Patel, and Nick Sweet for contributions of neuron tracings or review; Masayoshi Ito for identifying the R25C01-GAL4 line as expressing the WPN_Bs; Jay Milam for assistance with collecting confocal scans of the WPN_B transmitter colocalization; Tessa Cessario for fly care; Tyler Sizemore for assistance with recombining fly stocks; Quentin Gaudry for providing the UAS-RFP, LexAop-GFP;vGlut-LexA line; Tim Mosca for providing the UAS-Brp-short_mStraw, UAS-GFP; David Krantz for providing the hsFLP;;MCFO-1 line; Kevin Daly, Quentin Gaudry, Tim Mosca, and Tyler Sizemore for feedback on the manuscript; and Kevin Daly, Gary Marsat, and Keshav Ramachandra for insight on data analysis.