Figure 8. SWopto increases axonal sprouting during stroke recovery. A, Scheme of a brain coronal section 15 d after stroke (end point of experiment), representing tissue atrophy corresponding to the stroke area. Confocal micrography of iS1FL with 3D reconstruction of presynaptic and postsynaptic markers contact (right). Blue represents DAPI staining. Red represents Vglut1 presynaptic marker. Aquamarine represents PSD-95 postsynaptic marker. B, Comparison of Vglut1 puncta density between iS1FL and cS1FL cortical areas (mCherrystroke n = 4, ChR2stroke n = 4, ArchTstroke n = 4, mCherrysham n = 4) in ipsilateral (F(3,19) = 10.49, p = 0.0003) and contralateral layers II (F(3,19) = 1.069, p = 0.385), as well as in ipsilateral (F(3,18) = 16.02, p < 0.0001) and contralateral layers V (F(3,21) = 11.05, p = 0.0001) (C). D, Vglut1 puncta volume distribution in iS1FL layer II (F(2,1630) = 34.85, p < 0.0001) and layer V (E) summarized in bar graph (F(2,1617) = 155, p < 0.0001). F, Comparison of PSD-95 puncta density in ipsilateral (F(3,23) = 8.609, p = 0.0005) and contralateral (F(3,21) = 1.105, p = 0.369) layers II, as well as in ipsilateral (F(3,24) = 1.095, p = 0.370) and contralateral (F(3,24) = 2.498, p = 0.083) layers V (G). H, PSD-95 puncta volume distribution in iS1FL layer II summarized in bar graph (F(2,2070) = 9.164, p = 0.0001). I, iS1FL layer V (F(2,2111) = 75.13, p < 0.0001). One way-ANOVA. Data are mean ± SEM. *p < 0.05.