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Featured ArticleResearch Articles, Cellular/Molecular

Astrocytic IGF-IRs Induce Adenosine-Mediated Inhibitory Downregulation and Improve Sensory Discrimination

José Antonio Noriega-Prieto, Laura Eva Maglio, Jonathan A. Zegarra-Valdivia, Jaime Pignatelli, Ana M. Fernandez, Laura Martinez-Rachadell, Jansen Fernandes, Ángel Núñez, Alfonso Araque, Ignacio Torres-Alemán and David Fernández de Sevilla
Journal of Neuroscience 2 June 2021, 41 (22) 4768-4781; DOI: https://doi.org/10.1523/JNEUROSCI.0005-21.2021
José Antonio Noriega-Prieto
1Departamento de Anatomía, Histología y Neurociencia, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, Spain
7Department of Neuroscience, University of Minnesota, Minneapolis, Minnesota 55455
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Laura Eva Maglio
1Departamento de Anatomía, Histología y Neurociencia, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, Spain
2Departamento de Ciencias Médicas Básicas (Fisiología) and Instituto de Tecnologías Biomédicas, Universidad de La Laguna, 38071 San Cristóbal de La Laguna, Tenerife, Spain
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Jonathan A. Zegarra-Valdivia
3 Instituto Cajal, Consejo Superior de Investigaciones Científicas, 28002 Madrid, Spain
4Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas, 28031 Madrid, Spain
5Universidad Nacional de San Agustín de Arequipa, 04001 Arequipa, Perú
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Jaime Pignatelli
3 Instituto Cajal, Consejo Superior de Investigaciones Científicas, 28002 Madrid, Spain
4Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas, 28031 Madrid, Spain
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Ana M. Fernandez
3 Instituto Cajal, Consejo Superior de Investigaciones Científicas, 28002 Madrid, Spain
4Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas, 28031 Madrid, Spain
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Laura Martinez-Rachadell
3 Instituto Cajal, Consejo Superior de Investigaciones Científicas, 28002 Madrid, Spain
4Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas, 28031 Madrid, Spain
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Jansen Fernandes
3 Instituto Cajal, Consejo Superior de Investigaciones Científicas, 28002 Madrid, Spain
6Universidade Federal de São Paulo, São Paulo 04021-001, Brazil
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Ángel Núñez
1Departamento de Anatomía, Histología y Neurociencia, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, Spain
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Alfonso Araque
7Department of Neuroscience, University of Minnesota, Minneapolis, Minnesota 55455
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Ignacio Torres-Alemán
3 Instituto Cajal, Consejo Superior de Investigaciones Científicas, 28002 Madrid, Spain
4Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas, 28031 Madrid, Spain
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David Fernández de Sevilla
1Departamento de Anatomía, Histología y Neurociencia, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, Spain
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Abstract

Insulin-like growth factor-I (IGF-I) signaling plays a key role in learning and memory processes. While the effects of IGF-I on neurons have been studied extensively, the involvement of astrocytes in IGF-I signaling and the consequences on synaptic plasticity and animal behavior remain unknown. We have found that IGF-I induces long-term potentiation (LTPIGFI) of the postsynaptic potentials that is caused by a long-term depression of inhibitory synaptic transmission in mice. We have demonstrated that this long-lasting decrease in the inhibitory synaptic transmission is evoked by astrocytic activation through its IGF-I receptors (IGF-IRs). We show that LTPIGFI not only increases the output of pyramidal neurons, but also favors the NMDAR-dependent LTP, resulting in the crucial information processing at the barrel cortex since specific deletion of IGF-IR in cortical astrocytes impairs the whisker discrimination task. Our work reveals a novel mechanism and functional consequences of IGF-I signaling on cortical inhibitory synaptic plasticity and animal behavior, revealing that astrocytes are key elements in these processes.

SIGNIFICANCE STATEMENT Insulin-like growth factor-I (IGF-I) signaling plays key regulatory roles in multiple processes of brain physiology, such as learning and memory. Yet, the underlying mechanisms remain largely undefined. Here we demonstrate that astrocytes respond to IGF-I signaling, elevating their intracellular Ca2+ and stimulating the release of ATP/adenosine, which triggers the LTD of cortical inhibitory synapses, thus regulating the behavioral task performance related to cortical sensory information processing. Therefore, the present work represents a major conceptual advance in our knowledge of the cellular basis of IGF-I signaling in brain function, by including for the first time astrocytes as key mediators of IGF-I actions on synaptic plasticity, cortical sensory information discrimination and animal behavior.

  • astrocytes
  • barrel cortex
  • IGF-I
  • long-term depression
  • long-term potentiation
  • sensory discrimination

Introduction

Insulin-like growth factor-I (IGF-I) is a peptide involved in learning and memory (Ross, 2005; Aleman and Torres-Alemán, 2009). Indeed, a deficiency in circulating IGF-I results (Nishijima et al., 2010) in an impairment of the processing speed and deficiencies in both spatial and working memories (Deijen et al., 1996; Lijffijt et al., 2003; Sytze van Dam, 2005; Koltowska-Häggström et al., 2006; Adamsky et al., 2018), whereas replacing IGF-I reverses many of these cognitive deficits (Trejo et al., 2007). IGF-I regulates neuronal firing (Nuñez et al., 2003; Gazit et al., 2016) and modulates excitatory synaptic transmission in many areas of the brain (Nilsson et al., 1988; Araujo et al., 1989; Castro-Alamancos and Torres-Aleman, 1993; Seto et al., 2002; Maya-Vetencourt et al., 2012). IGF-I produces a long-lasting depression of glutamate-mediated GABA release by Purkinje cells in the cerebellum (Castro-Alamancos and Torres-Aleman, 1993) or a long-term potentiation (LTP) of GABA release in the olfactory bulb (Liu et al., 2017). However, whether IGF-I is involved in the long-lasting plasticity of inhibitory synaptic transmission in the neocortex remains unexplored.

The level of IGF-I modulates the plasticity of the somatosensory cortex in rats exposed to sensory motor restriction (Mysoet et al., 2015). The barrel cortex is the area of the somatosensory cortex involved in processing tactile information coming from the vibrissae, which is an extremely important factor in survival (Carvell and Simons, 1990). Interestingly, the uptake of IGF-I by the brain correlates with frequency-dependent changes in cerebral blood flow in the barrel cortex during whisker stimulation (Nishijima et al., 2010), indicating that IGF-I-induced plasticity at the barrel cortex is crucial in information processing in this cortical area. Moreover, somatosensory cortex astrocytes respond to sensory stimuli and regulate the sensory-evoked neuronal network (Lines et al., 2020). Indeed, astrocytes are of key importance in the induction of the long-term modulation of excitatory synaptic transmission induced by the spike timing-dependent protocol (Andrade-Talavera et al., 2016). They respond with Ca2+ elevations to neurotransmitters released by neurons and induce changes in neuronal excitability and synaptic transmission by releasing gliotransmitters (Araque et al., 2001; Nedergaard et al., 2003; Volterra and Meldolesi, 2005; Haydon and Carmignoto, 2006; Perea et al., 2009; Parpura and Zorec, 2010; Singh et al., 2014). In addition to glutamate, astrocytes also release ATP that in turn depresses excitatory synaptic transmission in the hippocampus (Serrano et al., 2006; Andersson et al., 2007; Chen et al., 2013). Moreover, in the neocortex, the exocytosis of ATP from astrocytes leads to a short-term downregulation of inhibitory synaptic currents by inhibiting postsynaptic and extrasynaptic GABAA receptors in layer II/III pyramidal neurons (Lalo et al., 2014). Further, astrocytes can release IGF-I (Chisholm and Sohrabji, 2016), and several studies have shown the presence of IGF-I and IGF-I receptors (IGF-IRs) in neurons, astrocytes, and microglia (Ocrant et al., 1988; Zhou et al., 1999; Garwood et al., 2015; Rodriguez-Perez et al., 2016). However, the effect of astrocytic IGF-IR activation on the long-term synaptic plasticity in the barrel cortex is yet to be elucidated.

Here, we have investigated whether astrocytic IGF-IR activation is participating in long-term synaptic transmission changes. We found that IGF-I induces an LTP of the postsynaptic potentials [PSPs (LTPIGFI)] that matches a presynaptic long-term depression (LTD) of IPSCs that depend on an increase in cytosolic calcium in the astrocytes, and the activation of A2A adenosine receptors. This LTD is absent in mice in which the IGF-IR has been deleted specifically in astrocytes (IGF-IR−/− mice). We show that the LTPIGFI favors the NMDAR-dependent LTP of the PSPs, in contrast to IGF-IR−/− mice that show impairment in their performance of a whisker discrimination task. We show that the activation of astrocytes by IGF-I is essential for inducing Hebbian plasticity. Therefore, we present a novel mechanism of long-term synaptic depression of inhibition at the barrel cortex induced by the activation of astrocytic IGF-IRs and ATP/adenosine (ATP/Ado) release from astrocytes that have an important impact on the processing of somatosensory information occurring during the whisker discrimination task.

Materials and Methods

Materials

2-(2-Furanyl)−7-(2-phenylethyl)-7H-pyrazolo[4,3-e][1,2,4] triazolo [1,5-c] pyrimidin-5-amine [SCH58261 (SCH), 100 nm], 8-cyclopentyltheophylline (CPT; 5 µm) and BAPTA-AM (acetyloxymethyl ester; 10 µm) were purchased from Tocris Bioscience. NVP AEW541 (NVP; 400 nm) was purchased from Cayman Chemical. Recombinant human IGF-I (IGF-I; 10 nm) was from Peprotech. BAPTA-K4 (40 mm) was from Santa Cruz Biotechnology. Fluo-4-AM (2 µm) was purchased from Thermo Fisher Scientific, and the ATP Assay Kit was from Abcam. All the remaining drugs were purchased from Sigma-Aldrich (CNQX, 20 µm; d-AP5, 50 m).

Key resources table

Bacterial and virus strains.

The bacterial and virus strains used were as follows: AAV8-GFAP-mCherry [3.13 × 1013 genome copies (GC)/ml] and AAV8-GFAP-mCherry-CRE (3.8 × 1012 GC/ml) were from University of Minnesota (UMN) Viral Vector and Cloning Core; and AAV5-pZac2.1-gfaABC1D-cyto-GCAMP6f (7 × 1012 viral genomes/ml) were from the Penn Vector Core.

Experimental models: organisms/strains.

The organisms and strains used were as follows: IGF-IR flox/flox (stock #012251, The Jackson Laboratory); and CreERT2.GFAP (stock #012849, The Jackson Laboratory).

Ethics statement and animals

All animal procedures were approved by the Ethical Committee of the Universidad Autónoma of Madrid, Cajal Institute, and the University of Minnesota Animal Care and Use Committee and are in accordance with Spanish Directives (R.D. 1201/2005) and European Community Directives (86/609/EEC and 2003/65/EC), and the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals. Male C57BL/6J or transgenic mice were housed under a 12 h light/dark cycle with up to five animals per cage and were used for slice electrophysiology. Transgenic mice with a deletion of IGF-IR in astrocytes (GFAP-IGF-IR−/− mice) were obtained by injecting IGF-IRf/f mice (B6, 129 background; The Jackson Laboratory) with AAV8-GFAP-mCherry (UMN Viral Vector and Cloning Core) or AAV8-GFAP-mCherry-CRE viral vectors (UNC Vector Core). In the Behavioral studies, adult male and female control mice (C57BL/6JolaHsd; age, 6–12 months; weight, 28–35 g) and transgenic mice were used. Transgenic mice with tamoxifen-regulated deletion of IGF-IR in astrocytes (IGF-IR−/− mice) were obtained by crossing IGF-IRf/f mice (B6, 129 background; IGF-IRflox/flox mice; stock #012251, The Jackson Laboratory) with CreERT2.GFAP mice (C57&B/6xSJL/J mixed background; stock #012849, The Jackson Laboratory; Ganat et al., 2006).

Slice preparation

Barrel cortex slices were prepared as described previously (Díez-García, 2017). Briefly, the mice were killed, and their brains submerged in cold (4°C) cutting solution containing the following (in mm): 189.0 sucrose, 10.0 glucose, 26.0 NaHCO3, 3.0 KCl, 5.0 Mg2SO4, 0.1 CaCl2, and 1.25 NaH2PO4.2H2O. Coronal slices (350 µm thick) were cut using a vibratome (model VT 1200S, Leica), and incubated (>1 h, at 25–27°C) in artificial CSF (ACSF) containing the following (in mm): 124.00 NaCl, 2.69 KCl, 1.25 KH2PO4, 2.00 Mg2SO4, 26.00 NaHCO3, 2.00 CaCl2, and 10.00 glucose). The pH was stabilized at 7.3 with a 95% O2, 5% CO2 mixture. The slices were transferred to a 2 ml chamber fixed to an upright microscope stage (model BX51WI, Olympus) equipped with infrared differential interference contrast (DIC) video microscopy and superfused at room temperature with 95% O2, 5% CO2-bubbled ACSF (2 ml/min).

Electrophysiological recordings in slices

Patch-clamp recordings from layer II/III pyramidal neurons of the barrel cortex were performed in whole-cell voltage-clamp and current-clamp configurations, as described previously (Díez-García, 2017). Briefly, patch pipettes (4–8 MΩ) were filled with an internal solution that contained the following (in mm): 135 KMeSO4, 10 KCl, 10 HEPES-K, 5 NaCl, 2.5 ATP-Mg+2, and 0.3 GTP-Na+, pH 7.3. In some experiments, the following BAPTA-based intracellular solution was used (in mm): 40 BAPTA-K4, 2 ATP-Na2+, 10 mm HEPES, 1 MgCl2, and 8 NaCl, pH 7.3. The holding potential was adjusted in a range from −70 to −80 mV, and series resistance was compensated to ∼80%. Layer II/III pyramidal neurons located over the barrels (layer IV) were accepted only when the seal resistance was >1 GΩ and the series resistance (10–20 MΩ) did not change (>20%) during the experiment. IPSCs were isolated in the presence of AMPAR and NMDAR antagonists (20 µm CNQX and 50 µm d-AP5, respectively). IPSCs were evoked with a bipolar stimulation electrode pulled from a theta borosilicate glass capillary (tip diameter, 5–8 µm), filled with ACSF, and connected to a stimulator (model S88, Grass) and a stimulus isolation unit (Quincy Digital) through chloride-silver electrodes. The stimulating electrodes were placed at layer IV of the barrel cortex near the tip (∼100–150 µm) of the recording pipette. Paired pulses (duration, 200 µs; interval, 50 ms) were continuously delivered at 0.33 Hz. After recording 5 min of stable baseline of the IPSCs, IGF-I was added to the bath for 35 min. To check for the induction of long-lasting synaptic plasticity, the IGF-I was washed for at least 10 min and the amplitude of the IPSCs after IGF-I washout was continuously checked. The presynaptic or postsynaptic origin of the observed regulation of synaptic currents was tested by estimating changes in the paired-pulse ratio (PPR) of the IPSCs (Creager et al., 1980; Clark et al., 1994; Kuhnt and Voronin, 1994). To estimate the modification of the synaptic current variance, we calculated the noise-free coefficient of variation of the synaptic responses before (control conditions) and during IGF-I (Maglio et al., 2018).

Electrophysiological recording in vivo

We studied 3-month-old male C57BL6/J mice weighing 25–30 g. Mice were group housed in clear plastic cages under a 12 h light/dark cycle and given ad libitum access to food and water. Surgery and recording were performed as previously described (Díez-García, 2017). Briefly, the mice were placed in a stereotaxic device under urethane (1.4 g/kg) in which surgical procedures and recordings were made. Local field potentials (LFPs) were obtained through tungsten microelectrodes (0.5–1 MΩ) located into the cortex. Signals were filtered (0.3–300 Hz for electrocorticogram) and amplified via a preamplifier (model AC P15, Grass). After a stable baseline recording, IGF-I or saline (six applications of 84 nl, one every 5 min, at a speed of 84 nl/min) was administered intracranially with a syringe (Gas Tight, Hamilton) coupled to a pump controller (Microinjection Syringe Pump Controller, WPI) stereostaxically placed at layer II/III of the barrel cortex.

Stereotaxic surgery and virus delivery

Mice between 8 and 10 weeks of age were anesthetized with a ketamine (100 mg/kg)/xylazine (10 mg/kg) mix. To selectively delete the IGF-IRs in cortical astrocytes, IGF-IRf/f mice were injected in the barrel cortex with 500 nl of the viral vector AAV8-GFAP-mCherry-CRE (or AAV8-GFAP-mCherry as a control). To monitor the astrocyte calcium levels, the mice were injected with 500 nl of AAV5-pZac2.1-gfaABC1D-cyto-GCAMP6f (Penn Vector Core). Injections were made at layer II/III of barrel cortex with a Hamilton syringe attached to a 29 gauge needle at a rate of 0.1 µl/min. The coordinates used to reach the area were as follows: anteroposterior, −1.0 mm; mediolateral, ±3.50 mm; dorsoventral, −0.15 mm. Three weeks after the injection, successful delivery was confirmed by the location of the virus based on mCherry expression. Immunohistochemistry analysis confirmed the selective expression of the virus in cortical astrocytes.

Ca2+ imaging

In GFAP-IGF-IR−/− mice and their controls, Ca2+ levels in the astrocyte soma and processes were obtained by two-photon microscopy (model DM6000 CFS upright multiphoton microscope with TCS SP5 MP laser, Leica) using the GCaMP6f viral vector targeted at astrocytes. In some experiments, the somatic Ca2+ levels were obtained using epifluorescence microscopy and the Ca2+ indicator Fluor-4-AM (5 µm in 0.01% pluronic, incubated for 30–45 min at room temperature). Loaded cells were illuminated every 100 ms at 490 nm with a monochromator (Polychrome IV, T.I.L.L. Photonics), and successive images were obtained at 1 Hz with a cooled monochrome CCD camera (Hamamatsu) attached to the Olympus microscope equipped with a filter cube (Chroma Technology). The camera control and synchronization for the epifluorescence were made by ImagingWorkbench software (INDEC BioSystems) and for two-photon imaging by LAS software (Leica).

Astrocyte cultures and ATP release

Pure astroglial cultures were prepared as described previously (Fernandez et al., 2007). Postnatal brains [postnatal day 0 (P0) to P2] from C57BL/6J [wild type (WT)] and constitutively GFAP IGF-IR−/− mice (Hernandez-Garzón et al., 2016) were removed and immersed in ice-cold Earle's balanced salt solution (Thermo Fisher Scientific). The cortices were dissected and mechanically dissociated. The resulting cell suspension was centrifuged and plated in DMEM/F-12 (Thermo Fisher Scientific) with 10% fetal bovine serum (FBS; Thermo Fisher Scientific) and a 100 mg/ml antibiotic-antimycotic solution (Sigma-Aldrich). Cell cultures were maintained in an incubator at 37°C and 95% humidity with 5% CO2. Pure astrocyte monolayer cultures were replated at 2.5 × 105 cells/well in DMEM/F-12 with 10% FBS medium. After 2 d, the medium was replaced by DMEM/F-12 for 3 h followed by a 1 h treatment with 10 nm IGF-I (PreproTech) or a 1 h treatment with the following inhibitors: 400 nm NVP-AEW541 (IGF-I receptor antagonist, Cayman Chemical), 10 µm BAPTA-AM (intercellular Ca2+ chelator, Sigma-Aldrich), followed by a 1 h stimulation with 10 nm IGF-I.

The amount of ATP released into the medium was measured using an ATP Assay Kit (fluorometric; catalog #ab83355, Abcam) according to the manufacturer instructions. First, the cells were washed with cold PBS and resuspended in 100 µl of ATP assay buffer. Then the cells were quickly homogenized by pipetting up and down and centrifuged for 2 min at 4°C (18,000 × g) to remove any insoluble material. The supernatant was collected and transferred to a clean tube and kept on ice. An ATP assay buffer (400 µl) and 100 µl of ice-cold 4 m perchloric acid were added to the homogenate to deproteinate the samples. The homogenates were briefly vortexed and incubated on ice for 5 min, centrifuged at 18,000 × g for 2 min at 4°C, and the supernatant was transferred to a fresh tube. The supernatant volume was measured, and an equal volume of ice-cold 2 m KOH was added. Finally, the homogenate was centrifuged at 18,000 × g for 15 min at 4°C, and the supernatant collected. ATP reaction mix (50 µl) and 50 µl of sample were added to each well and incubated at 24°C for 30 min, protected from light. The samples were measured on a FLUOstar microplate reader (BMG LabTech) at 535/587 nm. Each sample was run in duplicate. The concentrations of ATP released from the astrocyte primary cultures were obtained from standard curves and normalized to the total amount of protein.

Behavioral experiments

Adult male and female control (C57BL/6JolaHsd; age, 6–12 months; weight, 28–35 g), and transgenic mice with tamoxifen-regulated deletion of IGF-IR in astrocytes (IGF-IR−/− mice; see above) were used. The IGF-IR activity was knocked down after the administration of tamoxifen, as described previously (Hirrlinger et al., 2006). Tamoxifen was injected for 5 consecutive days intraperitoneally (75 mg/kg; Sigma-Aldrich) from the age of 1 month, and the mice were used at 2–7 months of age. The controls used were their siblings that had been treated only with the vehicle for tamoxifen injections (corn oil). Using the tdTomato/eGFP reporter mouse to detect Cre-mediated deletion in response to tamoxifen administration in CreERT2.GFAP mice, we previously documented that it was restricted to the astrocytes (García-Cáceres et al., 2016). Multiplex PCR for mouse genotyping included a common forward primer (P3: 5'−CTG TTT ACC ATG GCT GAG ATC TC − 3'), and two reverse primers specific for the wild-type (P4: 5'−CCA AGG ATA TAA CAG ACA CCA TT − 3') and mutant (P2: 5′-CGC CTC CCC TAC CCG GTA GAA TTC-3') alleles. The GFAP-T-IGF-IR mice showed lower levels of IGF-IR in their brains after tamoxifen injection. The mice were housed in standard cages (48 × 26 cm2) with four to five animals per cage, kept in a room with controlled temperature (22°C) under a 12 h light/dark cycle, and fed with a pellet rodent diet and water available ad libitum. All experimental protocols were performed during the light cycle between 14 and 18 p.m. The animal procedures followed European guidelines (2010/63/EU) and were approved by the local Bioethics Committee (Madrid Government).

qPCR.

Total RNA isolation from their brain tissue was conducted with TRIzol. One milligram of RNA was reverse transcribed using a high-capacity cDNA reverse transcription kit (Thermo Fisher Scientific) according to the manufacturer instructions. cDNA (62.5 ng) was amplified using TaqMan probes for mouse IGF-IR, and rRNA 18S as the endogenous control (Thermo Fisher Scientific). Each sample was run in triplicate in 20 µl of reaction volume using a TaqMan Universal PCR Master Mix according to the manufacturer instructions (Thermo Fisher Scientific). All the reactions were performed in a 7500 Real Time PCR System (Thermo Fisher Scientific). Quantitative real-time PCR analysis was conducted as described previously (Pfaffl, 2001). The results were expressed as relative expression ratios on the basis of group means for target transcripts versus the reference 18S transcript.

For the behavioral tests, both sexes were used and balanced between experimental groups. Since the wild-type mice and their control littermates performed similarly in the tests, they were pooled and presented as a single control group. We performed the following three behavioral tests.

Gap-crossing test.

To assess sensory perception through the whiskers, we used the gap-crossing test, which consists of a series of trials in which the mouse has to cross a gap with increasing distances (Hutson and Masterton, 1986; Barnéoud et al., 1991). Each mouse was placed in the center of an elevated platform (5 cm wide, 9 cm long) connected to a safe black cylindrical tube (diameter, 8 cm; length, 9 cm). The gap distance between the platform and the cylindrical tube ranged from 0 to 8 cm in 1 cm increments. The test was performed under infrared lighting, the trials were recorded with a video camera, and the maximum distance crossed by each animal was measured.

Y-maze spontaneous alternation test.

Working memory was assessed by recording spontaneous activity while exploring a Y-maze (Sarter et al., 1988). The maze was made of black-painted wood, and each arm was 25 cm long, 14 cm high, 5 cm wide, and positioned at equal angles. The trials lasted 8 min each. After each trial, the maze was cleaned with 70% ethanol to remove any olfactory cues. An off-line analysis of the videos was carried out to obtain the sequence of entries during the test. Alternate behavior was calculated as the percentage of real alternations (number of triplets with nonrepeated entries) versus total alternation opportunities (total number of triplets).

Whisker discrimination test.

To assess the ability of the mice to discriminate different textures with their whiskers, we adapted the two-trial Y-maze test described in previous work (Dellu et al., 2000; Hausrat et al., 2015). The apparatus was constructed of black-painted wood with three arms, each 25 cm long, 5 cm wide, and 14 cm high. The walls of the maze arms were covered with two different grades of black sandpaper. While two arms (familiar) were covered with a 500 grit sandpaper, the third (novel) was covered with 220 grit sandpaper. Because the three arms of the maze were identical, and there are no extramaze cues, the discrimination of novelty versus familiarity relies only on the different textures that the mouse can perceive with its whiskers. The experiments were conducted in a room with dim illumination (6 lux). During the acquisition phase, the mice were placed at the end of one of the familiar arms (in a random order) and were allowed to explore both familiar arms (500 grit sandpaper) for 5 min while the third arm (novel; 220 grit sandpaper) was closed with a guillotine door. At the end of the first trial, the mice were returned to their home cage for 5 min. In the retrieval phase, the mice were again placed at the end of the same arm where they started the acquisition phase and were allowed to freely explore all three arms for 5 min. To remove any possible olfactory cues, the maze was cleaned with 70% ethanol between the trials. The time spent in each of the arms was recorded using a video camera, and the discrimination index [novel arm/(novel + familiar arms)] × 100 calculated.

Data analysis

Electrophysiological data analysis was conducted in Clampfit 10 (Axon Instrument), and ImageJ (NIH) was used for the calcium imaging. The graphs were drawn in SigmaPlot 11 (Systat Software). The fluorescence values are given as ΔF/F0 (ΔF/F0 = 100 × (F – F0)/F0), where F0 is the prestimulus fluorescence level when cells were at rest, and F is the fluorescence at different times during activity. Data normality was tested using a Kolmogorov–Smirnov test. We used the Student's two-tailed t tests for unpaired or paired data as required in the electrophysiology experiments. Data are presented as the mean ± SEM. The thresholds for statistical significance were *p < 0.05, **p < 0.01, and ***p < 0.001 for the Student's test. Statistical analysis was performed using GraphPad Prism 5.0. To compare differences between two groups and compare multiple variables, two-way ANOVA, one-way ANOVA (parametric test) or Kruskal -Wallis tests (nonparametric test) are used, followed by Holm-Sidak and Dunn tests (for parametric and nonparametric test, respectively) or Bonferroni post hoc test to compare replicate means. Statistical differences were considered at p < 0.05. The results are presented as the mean ± SEM of five independent experiments (#p < 0.05, ##p < 0.01, ###p < 0.001).

Results

IGF-I induces LTP of PSPs (LTPIGFI) at layer II/III pyramidal neurons

The experience-dependent plasticity of barrel cortex layer IV neurons is largest when the experience is manipulated early (before P7), but the plasticity of layer II/III responses persists beyond the first week of life (Fox, 1992). During the second postnatal week, the layer IV map serves as a template for the growth and Hebbian refinement of the cortical circuitry underlying the layer II/III map, and layer IV to layer II/III feedforward excitatory projections are confined almost exclusively to one barrel column (Lübke et al., 2000). Therefore, we investigated whether IGF-I modulates the efficacy of these synapses. We recorded the PSPs at layer II/III pyramidal neurons evoked by the stimulation of layer IV (Fig. 1A). After recording 5 min of stable baseline of the IPSCs, IGF-I was added to the bath for 35 min in an attempt to mimic the long-lasting increase in serum IGF-I observed after a long-term resistance exercise (Tsai et al., 2015) and the associated increase in the IGF-I levels in the brain (Carro et al., 2000). After 5 min of stable recording, 10 nm IGF-I was applied for 35 min. The addition of IGF-I increased the number of synaptic stimuli that evoked action potentials (APs; from 6.5 ± 2.1 basal to 32.6 ± 4.5 after 15 min of IGF-I application; Fig. 1B, ###p = 0.0004). In addition, it also induced a potentiation of the PSPs that remained for 30 min after the IGF-I washout (percentage of peak amplitude: from 100.70 ± 1.58% basal to 146.80 ± 9.06% at 60 min after IGF-I application; Fig. 1B, **p = 0.0014). These IGF-I-induced effects were prevented by bath perfusion of the IGF-IR-selective inhibitor NVP-AEW 541 [number of Aps: from 6.4 ± 0.5 basal to 12.4 ± 2.1 at 15 min after IGF-I application (p = 0.101); percentage of peak amplitude: from 97.00 ± 1.87% basal to 103.60 ± 12.27% at 60 min after IGF-I application (Fig. 1B, p = 0.6472)], indicating that they were mediated by IGF-IR activation. Therefore, activation of IGF-IR induces an LTP of the PSPs (termed LTPIGFI).

Figure 1.
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Figure 1.

IGF-I induces LTP of the PSPs at layer II/III pyramidal neuron of barrel cortex. A, DIC image showing the recording (Rec.) and the stimulation (Stim.) electrodes in a slice. Scale bar, 100 µm. B, Top, Representatives PSPs in control [black, left, (1)] and during IGF-I exposure [red, right, (4)]. In the middle, representative AP responses before IGF-I [black (2), 6 of 60 stimuli] and during IGF-I [red (3), 32 of 60 stimuli]. Bottom, Left, Time course of the PSP before, during, and after washing out the IGF-I in ACSF (full circles; n = 8 cells/3 animals; control vs IGF-I, **p < 0.01), and in the presence of NVP-AEW 541 [empty circles; n = 5 cells/2 animals; NVP vs NVP+IGF-I, ns (nonsignificant)]. Two-tailed Student's paired t test. Right, Number of APs every 5 min in before and during the first 15 min of IGF-I exposure in ACSF (filled bars; n = 8 cells/3 animals; control vs IGF-I; ##p < 0.01, ###p < 0.001; F(3,28) = 8.757) and in the presence of NVP-AEW 541 (empty bars; n = 5 cells/2 animals; NVP vs NVP+IGF-I, ns; F(3,16) = 2.687) One-way ANOVA. C, Top, Time course scheme showing the local injections of saline or IGF-I (arrows) during LFP recordings in layer II/III of the barrel cortex in vivo. Bottom, Drawing illustrating the recording electrode and Hamilton syringe location, and air puff stimulation. D, Left, Time course of LFP recorded in mice injected (arrows) with IGF-I (red; n = 10 animals; control vs IGF-I, ***p < 0.001) and saline (black; n = 9 animals; control vs saline, ns) two-tailed Student's paired t test. Right, Representative superimposed LFPs recorded in the control [black, (1)] and after 80 min of the injections [gray, (2)] in mice injected with saline (top, LFP saline) and mice injected with IGF-I (bottom, LFP IGF-I). Note that IGF-I induce LTP of the synaptic potentials recorded at layer II/III of the barrel cortex both in vivo and in the slices.

To test whether the LTP can be also induced in vivo, we recorded the extracellular LFPs at layer II/III of the barrel cortex evoked by low-frequency deflections delivered at the principal whisker (Fig. 1C). Following 15 min of control whisker-evoked LFP recording, IGF-I was applied in six IGF-I applications, one injection of 84 nl IGF-I (0.076 mg/ml) every 5 min via a Hamilton syringe placed close to the recording electrode (see Materials and Methods). IGF-I application induced a long-lasting enhancement of the LFP (percentage of LFP area: from 99.45 ± 1.80% basal to 177.60 ± 21.00% at 80 min after IGF-I application; Fig. 1D, ***p = 0.0002). This enhancement in the synaptic efficacy was absent in the controls using a saline injection (percentage of LFP area: from 99.94 ± 0.33% basal to 90.98 ± 10.74% at 80 min after IGF-I application; Fig. 1D, p = 0.4317). Therefore, these results suggest that IGF-I induces the LTP of the synaptic potentials at layer II/III pyramidal neurons both ex vivo and in vivo.

Activation of IGF-IR in astrocytes induces presynaptic LTD of IPSCs (LTDIGFI)

The increase in the efficacy of the synaptic potentials could be because of the modulation of the inhibitory and/or excitatory synaptic transmission. To elucidate this issue, we first investigated whether IGF-I reduces the efficacy of inhibitory synaptic transmission. IPSCs were isolated in the presence of the glutamate receptor antagonists 20 µm CNQX and 50 µm AP5. After 5 min of IPSC recording, IGF-I was bath applied for 35 min and then washed out. The IGF-I induced an LTD of the IPSC peak amplitude (termed LTDIGFI) that persisted after IGF-I washout (percentage of peak amplitude: from 100.60 ± 1.42% basal to 60.73 ± 5.08% at 60 min after IGF-I application; Fig. 2A,B, ***p = 0.0002). The IGF-I-induced effects were prevented by NVP-AEW 541 (percentage of peak amplitude: from 98.72 ± 0.50% basal to 96.15 ± 3.59% at 60 min after IGF-I application; Fig. 2A, p = 0.9881), indicating that they were mediated by IGF-IR activation. Because postsynaptic calcium increases are known to be required for the induction of long-term synaptic plasticity (Zucker, 1999), we then tested whether LTDIGFI induction required cytosolic calcium elevation in the recorded neuron. Therefore, we included the Ca2+chelator BAPTA (40 mm) in the patch pipette to prevent neuronal calcium elevations. However, a similar LTDIGFI was induced in both the control and the neuron BAPTA-loaded conditions [percentage of peak amplitude: from 100.08 ± 0.60% basal to 68.67 ± 6.76% at 60 min after IGF-I application; Fig. 2C, BAPTAn (BAPTA in the postsynaptic neuron), **p = 0.0066]. To test the possibility of a presynaptic origin for the effect of IGF-I, we recorded the IPSCs evoked by paired-pulse stimulation (50 ms delay) and analyzed changes in PPRs. The PPRs were increased during LTDIGFI recorded both in the control condition and during the BAPTA loading in neurons [percentage of PPR: from 100.00 ± 3.23% basal to 120.37 ± 5.33% (***p = 0.0002) in control; and from 100.00 ± 3.40% basal to 114.34 ± 4.59% at 60 min after IGF-I application (**p = 0.0015), in postsynaptic BAPTA condition; Fig. 2D, control and BAPTAn], indicating the presynaptic origin of the LTDIGFI. This increase in the PPR was prevented by NVP (percentage of PPR: from 100.00 ± 3.23% basal to 99.95 ± 14.00% at 60 min after IGF-I application; p = 0.9636; Fig. 2D, NVP). Together, these data suggest that IGF-I induces a presynaptic LTD of IPSCs, which do not require cytosolic calcium elevations in the recorded postsynaptic neuron.

Figure 2.
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Figure 2.

Astrocytic IGF-IR activation induces adenosine-mediated LTD of the IPSCs. A, Time course of the IPSC peak amplitude before, during, and after washing out of IGF-I in ASCF (control, empty circles; n = 7 cells/4 animals; control vs IGF-I, **p < 0.001), and in the presence of NVP-AEW 541 [gray circles; n = 7 cells/3 animals; NVP vs NVP+IGF-I, ns (nonsignificant), two-tailed Student's paired t test]. Right, Representative IPSCs in control (gray) and after 20 min of IGF-I washout (red). B, Cartoon showing that the LTD of the IPSCs induced by IGF-I in layer II/III pyramidal neurons (green cell) depends on the IGF-IR activation on astrocytes (orange cell with blue processes) and the adenosine receptor-mediated reduction of GABA release form the GABAergic interneurons (gray cell). C, Effect of IGF-I on the IPSC peak amplitude (red bar), as a percentage of the control (gray bar), in different experimental conditions, as follows: ACSF, NVP, BAPTAn (n = 6 cells/3 animals; control vs IGF-I, **p < 0.01), BAPTA loaded astrocyte (BAPTAa; n = 6 cells/3 animals; control vs IGF-I, **p < 0.01), ACSF in the IGF-IR−/− mice (n = 5 cells/2 animals; control vs IGF-I, ns) and its littermates (IGF-IR+/+; n = 5 cells/2 animals; control vs IGF-I, *p < 0.05), MPEP+LY 367385 (n = 6 cells/3 animals; MPEP+LY vs MPEP+LY+IGF-I, **p < 0.01), SCH (n = 8 cells/4 animals; SCH vs SCH+IGF-I, ns), and CPT (n = 7 cells/2 animals; CPT vs CPT+IGF-I, ***p < 0.001, two-tailed Student's paired t test). Note that the LTD of the IPSCs by IGF-I is prevented when IGF-IRs are absent on astrocytes or when they are filled with BAPTA, and when adenosine A2 receptors or IGF-I receptors are blocked by CPT and NVP, respectively. D, Left, Effect of IGF-I on the IPSC PPR (red bar), as a percentage of the control (gray bar), in the same different experimental conditions of C. Right, Representative IPSCs evoked by paired pulses recorded before (gray trace, control) and during IGF-I (red trace, IGF-I). Note that IGF-I increases the PPR (R2/R1) because it reduces the first IPSC (R1) more than the second IPSC (R2). ###p < 0.001.

Astrocytes are emerging as important cells involved in the regulation of synaptic transmission and plasticity (Araque et al., 2014) that are able to regulate inhibitory synaptic transmission in the hippocampus (Kang et al., 1998). Therefore, we investigated whether LTDIGFI requires cytosolic calcium elevations in astrocytes. The application of IGF-I induced an increase in the frequency of astrocyte calcium elevations (from 0.71 ± 0.09 basal to 1.35 ± 0.12 after IGF-I application; Fig. 3A, control, **p = 0.0023) that was absent under NVP (from 0.98 ± 0.10 basal to 1.00 ± 0.05 after IGF-I application; p = 0.8909; Fig. 3A, NVP). We then tested whether the IGFI-induced enhancement of the astrocyte calcium signal contributed to the LTDIGFI. We prevented the Ca2+ signal selectively in astrocytes by recording a cortical astrocyte with a patch pipette containing 40 mm BAPTA. Since cortical astrocytes are gap junction connected, injecting BAPTA into a single astrocyte can diffuse through a large extension of the gap junction-coupled astrocytic network (Navarrete and Araque, 2010). However, BAPTA loading the astrocytic network, IGF-I failed to modulate the IPSCs [percentage of peak amplitude: from 99.79 ± 0.62% basal to 92.75 ± 6.20% at 60 min after IGF-I application; p = 0.3222; Fig. 2C, BAPTAa (BAPTA loaded astrocyte)] or the PPR (percentage of PPR: from 100.00 ± 3.86% basal to 98.01 ± 2.48% at 60 min after IGF-I application; p = 0.5896; Fig. 2D, BAPTAa). Therefore, these data suggest that cytosolic Ca2+ increases in the astrocytes, not in the postsynaptic neurons, are essential for the induction of LTDIGFI.

Figure 3.
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Figure 3.

Astrocytic IGF-IR activation stimulates the calcium-dependent release of ATP. A, Effect of IGF-I on the spontaneous Ca2+ event frequency of the astrocytes in different experimental conditions: in ACSF (control, n = 55 astrocytes/4 animals; control vs IGF-I, **p < 0.01), NVP (n = 65 astrocytes/3 animals; NVP vs NVP+IGF-I, ns), MPEP+LY (n = 44 astrocytes/3 animals; MPEP+LY vs MPEP+LY+IGF-I, **p < 0.01), SCH (n = 48 astrocytes/4 animals; SCH vs SCH+IGF-I, *p < 0.05), and CPT (n = 72 astrocytes/2 animals; CPT vs CPT+IGF-I, ***p < 0.001, two-tailed Student's paired t test). B, Representative images of locally targeting astrocytes with GFAP-GCaMP6f-mCherry for IGF-IR+/+ (top images) and GFAP-GCaMP6f-mCherry-CRE for IGF-IR−/− (bottom images). Scale bar, 50 µm. C, Pseudocolor Ca2+ images showing the intensities of GCaMP6f-expressing astrocytes in the barrel cortex, in the basal condition and during IGF-I exposure in IGF-IR+/+ (top images) and IGF-IR−/− astrocytes (bottom images). Scale bar, 50 µm. Right, Representative Ca2+ traces in the astrocytes from IGF-IR+/+ (top traces) and the IGF-IR−/− astrocytes (bottom traces). D, Spontaneous Ca2+ event frequency in the processes of the astrocytes in the basal condition and during IGF-I exposure in IGF-IR+/+ (n = 135 astrocytes/2 animals; control vs IGF-I, **p < 0.01) and IGF-IR−/− (n = 67 astrocytes/2 animals; control vs IGF-I, ns, two-tailed Student's paired t test) astrocytes. E, Bar plot of ATP concentration in a culture of astrocytes before (black bar) and during IGF-I exposure (red bar) in different experimental conditions: ACSF (n = 6; basal vs IGF-I, ***p < 0.001), NVP (n = 6; IGF-I vs NVP+IGF-I, ***p < 0.001), BAPTA-AM (n = 6; IGF-I vs BAPTA-AM + IGF-I, **p < 0.01), and in the astrocytes from the IGF-IR+/+ and IGF-IR−/− mice (n = 6; IGF-I+/+ vs IGF-I−/−, **p < 0.01, two-way ANOVA with post hoc Bonferroni multiple-comparisons test). ns, not significant, ##p < 0.01, ###p <0.001.

Although LTDIGFI requires astrocyte calcium elevations, astrocyte stimulation by IGF-I could occur directly either through the activation of astrocytic receptors, or indirectly through the activation of neuronal receptors that trigger an indirect signaling pathway. To discriminate between these two possibilities, we deleted IGF-IR specifically in astrocytes using a combined viral and genetic approach. Mice with floxed IGF-IR gene (IGF-IRf/f mice) were injected in the barrel cortex with the virus AAV8-GFAP-Cre-mCherry (Fig. 3B, bottom images) or AAV8-GFAP-mCherry as a control (Fig. 3B, top images) that included the Cre-recombinase under the astroglial promoter GFAP, and mCherry as a reporter. In IGF-IRf/f mice injected with AAV8-GFAP-Cre-mCherry, mCherry expression was largely reduced and confined to the soma (Fig. 2F, mCherry, IGF-IR−/−) compared with the higher and more spread signal in control littermates (Fig. 2F, mCherry, IGF-IR+/+). As expected, in the IGF-IR+/+ mice, IGF-I elevated astrocyte calcium levels (frequency of calcium event: from 1.60 ± 0.03 basal to 2.93 ± 0.05 after IGF-I application; p = 0.0054; Fig. 3C,H, IGF-IR+/+), inducing the LTDIGFI and increasing the PPR [percentage of peak amplitude: from 99.89 ± 0.80% basal to 74.74 ± 4.97% (*p = 0.0108) at 60 min after IGF-I application; percentage of PPR: from 100.00 ± 2.64% basal to 120.92 ± 1.31% at 60 min after IGF-I application; **p = 0.0034; Fig. 2C,D, IGF-IR+/+]. By contrast, in the IGF-IR−/− mice, IGF-I did not modify the frequency of calcium elevations in astrocytes (frequency of calcium event: from 1.23 ± 0.02 basal to 1.04 ± 0.004 after IGF-I application; p = 0.2128) or alter the IPSC amplitude and PPR [percentage of peak amplitude: from 100.27 ± 1.55% to 107.16 ± 5.62% (p = 0.3106); percentage of PPR: from 100.00 ± 5.36% to 102.92 ± 5.05% (p = 0.7014) basal and at 60 min after IGF-I application, respectively; Figs. 2C,D, 3D, IGF-IR−/−]. These results further support the idea that LTDIGFI depends on IGF-IR activation in the astrocytes.

LTDIGFI requires A2A adenosine but not mGluR receptor activation

Astrocytic activation stimulates the release of glutamate that regulates synaptic transmission by acting on metabotropic glutamate receptors (mGluRs; Perea and Araque, 2007). We therefore checked whether the LTDIGFI is mediated by the activation of mGluRs. However, LTDIGFI was unaffected (percentage of peak amplitude: from 98.89 ± 0.90% basal to 60.78 ± 7.39% at 60 min after IGF-I application; Fig. 2C, MPEP+LY, **p = 0.0022) by treatment with the antagonists of group I metabotropic glutamate receptors MPEP and LY367385, and the increase observed in the PPR induced by IGF-I was preserved (percentage of PPR: from 100.00 ± 4.06% basal to 120.52 ± 7.66% at 60 min after IGF-I application; Fig. 2D, MPEP+LY, *p = 0.024). Moreover, we observed that IGF-I evoked an increase in calcium event frequency in the presence of MPEP + LY367385 (frequency of calcium event: from 0.51 ± 0.12 basal to 1.07 ± 0.14 after IGF-I application; Fig. 3A, MPEP + LY, **p = 0.0066). Although astrocytes are able to release glutamate, these data rule out the requirement of mGluR activation in the induction of LTDIGFI.

Astrocytes may also release ATP, which, after being converted to adenosine, may regulate synaptic transmission (Panatier et al., 2011; Araque et al., 2014). Therefore, we tested whether astrocytic ATP/Ado was responsible for the LTDIGFI. The LTDIGFI was abolished by the antagonist of adenosine A2A receptors, antagonist SCH 58261 (percentage of peak amplitude: from 100.02 ± 0.63% basal to 94.98 ± 2.43% at 60 min after IGF-I application; p = 0.0916; Fig. 2C, SCH), but not by the antagonist of adenosine A1 receptors CPT (percentage of peak amplitude: from 98.72 ± 1.75% basal to 55.74 ± 7.08% at 60 min after IGF-I application; ***p = 0.0008; Fig. 2C, CPT). Moreover, the increase in PPR induced by IGF-I was preserved in the presence of CPT but was absent in SCH 58261 [from 100.00 ± 2.09 to 99.76 ± 1.85 (p = 0.8967); percentage of PPR: from 100.00 ± 5.90% to 118.42 ± 4.95% (**p = 0.0023) basal and at 60 min after IGF-I application in SCH and CPT, respectively; Fig. 2D, SCH, CPT]. To test whether adenosine receptor activation occurs downstream of astrocytic calcium activity, we analyzed the effects of A2A and A1 receptor antagonists on astrocytic calcium signals. We observed that IGF-I still evoked an increase in calcium event frequency in the presence of SCH and CPT [frequency of calcium event: from 0.67 ± 0.12 to 1.40 ± 0.17 (*p = 0.0194) and from 0.59 ± 0.10 to 1.20 ± 0.13 (***p = 0.0008) basal and after IGF-I application in SCH and CPT respectively; Fig. 3A, SCH, CPT]. Together, these results suggest that IGF-I stimulates the astrocytic release of ATP that induces LTDIGFI acting downstream of astrocytic calcium activity.

Therefore, we tested whether IGF-I was capable of stimulating the release of ATP from astrocytes. We used an ATP Assay Kit (see Materials and Methods) to monitor ATP levels in cultured astrocytes before and after 1 h of treatment with IGF-I (10 nm). We found that IGF-I elevated the extracellular levels of ATP (1.1 ± 0.04 nmol/µg; Fig. 3E, control, ***p < 0.001), an effect that was prevented when cultures where simultaneously treated with NVP (0.25 ± 0.05 nmol/µg; Fig. 3E, NVP). Furthermore, the IGF-I-induced release of ATP was absent when astrocyte calcium signaling was prevented by treating cultures with BAPTA-AM (0.45 ± 0.04 nmol/µg; Fig. 3E, BAPTA-AM). Moreover, ATP was not released when astrocytes were obtained from mice lacking IGF-IR. (1.1 ± 0.02 and 0.3 ± 0.01 nmol/µg in IGF-IR+/+ and IGF-IR−/−, respectively; Fig. 3E, IGF-IR−/−, IGF-IR+/+). These data suggest that IGF-I, acting through the IGF-IR in astrocytes, stimulates the calcium-dependent release of ATP, which leads to the activation of A2A receptors, thus inducing LTDIGFI.

IGF-IR activation induces a short-term potentiation of the EPSCs

The next step was to analyze whether IGF-I could also modulate the efficacy of excitatory synaptic transmission. To measure the effect of IGFI in excitatory synaptic transmission when the inhibition is present, we repeated the experiment illustrated in Figure 1B, but instead of recording the PSPs, we recorded the EPSCs at the reversal potential of the inhibitory synaptic transmission (−70 mV). In these experiments, as in Figure 1B, IGF-I still increased the number of synaptic stimuli that evoked APs (number of APs: from 8.0 ± 2.5 basal to 30.5 ± 4.8 at 15 min after IGF-I application; Fig. 4A, ##p = 0.0044). Moreover, IGF-I induced a significant short-term potentiation (STP) of the ESPCs (termed STPIGF-I; percentage of peak amplitude: from 102.00 ± 2.16% basal to 141.32 ± 9.10% at 20 min after IGF-I application; **p = 0.0011; Fig. 4A,B). This effect was prevented by the IGF-IR selective inhibitor NVP-AEW 541 [number of APs: from 4.4 ± 2.3 basal to 5.2 ± 3.5 at 15 min after IGF-I application (p = 0.1806); percentage of peak amplitude: from 99.10 ± 0.79% basal to 103.0 ± 3.02% at 20 min after IGF-I application; p = 0.2338; Fig. 4A,C). Since an increase in cytosolic Ca2+ in astrocytes is required for the induction of LTDIGFI (see above), we explored whether the induction of STPIGFI also depended on the activation of astrocytes. After BAPTA loading into the astrocytic network, IGF-I was not able to modulate the EPSCs (percentage of peak amplitude: from 95.10 ± 1.46% basal to 108.21 ± 10.51% at 20 min after IGF-I application; p = 0.2571; Fig. 4C, BAPTAa). These results confirm that the STPIGFI not only depends on IGF-IR activation in astrocytes, but also on calcium elevations in their cytosol.

Figure 4.
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Figure 4.

IGF-I generates a transient potentiation of the EPSCs. A, Top, Representative EPSC responses before [black, (1)], during IGF-I [red, (4)], and during IGF-I washout [gray, (5)]. IGF-I increases the number of AP responses recorded every 60 stimuli (green box), from 8 before IGF-I [black trace, (2)] to 30 during IGF-I [red trace, (3)]. Bottom, Left, Time course of the EPSC peak amplitude before, during IGF-I, and during IGF-I washout in ACSF (full circles), and in the presence of NVP-AEW 541 (empty circles). Note that during the green box, EPSC peak amplitude values are missed because we recorded the effect of IGF-I on the number of stimuli evoking AP responses. Right, Number of APs recorded every 5 min before (black) and during IGF-I (red) in ACSF (filled bars; n = 8 cells/3 animals; control vs IGF-I, #p < 0.05, ##p < 0.01; F(3,28) = 5.474) and in NVP (empty bars; n = 5 cells/2 animals; NVP vs NVP+IGF-I, ns; F(3,16) = 0.2402, one-way ANOVA). B, Cartoon showing that the STP of the EPSCs induced by IGF-I depends on the IGF-IR activation on astrocytes and the adenosine receptor-mediated increase of the release of glutamate from the glutamatergic terminal (blue terminal). C, Effect of IGF-I on the EPSC peak amplitude (green bar), as a percentage of the control (gray bar), in different experimental conditions: ACSF, NVP, BAPTAa (n = 5 cells/3 animals; control vs IGF-I, ns), and SCH (n = 6 cells/3 animals; SCH vs SCH+IGF-I, ns, two-tailed Student's paired t test). Note that the modulation of the EPSCs by IGF-I is prevented in the same conditions in which the modulation of the IPSCs by IGF-I was abolished. D, As in A, right bottom, but in BAPTA-loaded astrocyte (n = 5 cells/3 animals; control vs IGF-I, ns; F(3,16) = 0.2457) and SCH (n = 6 cells/3 animals; SCH vs SCH+IGF-I, ns; F(3,20) = 0.1302). One-way ANOVA. E, Plot of the variance (1/CV2r) as a function of the mean EPSC peak amplitude (M) in the control, BAPTA-loaded astrocyte, and SCH. Note that the control condition does not follow a diagonal, suggesting a postsynaptic origin of the transient potentiation. ns, not significant, **p < 0.01.

We then checked whether the potentiation of EPSCs by IGF-I requires ATP released by astrocytes. The EPSC modulation by IGF-I was abolished by the A2A receptor antagonist SCH (percentage of peak amplitude: from 98.42 ± 2.41% in basal to 90.20 ± 3.36% at 20 min after IGF-I application; p = 0.2084; Fig. 4C, SCH). In addition, IGF-I did not increase the number of APs during SCH or when BAPTA was loaded into the astrocytic network [number of APs: from 3.8 ± 1.1 to 3.6 ± 1.4 (p = 0.9941) basal and from 6.0 ± 1.2 to 8.0 ± 2.9 (p = 0.994) at 15 min after IGF-I application in BAPTAa and SCH, respectively; Fig. 4D]. To test the presynaptic or postsynaptic origin of the effect of IGF-I on the EPSCs, we constructed 1/CV2 plots (see experimental procedures). We observed that the increase in the mean EPSC peak amplitude was not paralleled by an increase in the 1/CV2 parameter, which suggests a postsynaptic origin of the EPSC potentiation (R2 = 0.0107; Fig. 4E, control). These data suggest that potentiation of the EPSCs induced by IGF-I was mediated by a postsynaptic mechanism that was absent in the recordings under BAPTA in astrocytes (Fig. 4E). These combined results indicate that IGF-I induces an increase in calcium levels in astrocytes, which leads to the activation of A2A receptors, thus inducing a postsynaptic STP of the EPSCs and a presynaptic LTD of the IPSCs, which would result in the LTP of the PSPs.

IGF-I controls the induction threshold of Hebbian LTP (LTPH)

Because IGF-I induces the LTP of the PSPs, it may have a key role in the modulation of Hebbian LTP (LTPH), decreasing its induction threshold. Therefore, we tested the impact of IGF-I on the LTPH induced by spike timing-dependent plasticity (STDP). First, we analyzed the LTPH induction threshold under the control conditions. We used STDP protocols consisting of a subthreshold PSP followed by a backpropagating AP at delays of 10 ms repeated 10, 20, and 50 times at 0.2 Hz. While 10 and 20 pairings were unable to induce the LTPH of the PSPs [percentage of peak amplitude: from 99.89 ± 0.21% to 100.03 ± 4.17% basal (p = 0.9758); from 101.88 ± 0.71% to 101.51 ± 2.79% (p = 0.8339) at 40 min after 10 and 20 pairings, respectively; Fig. 5A, blue squares and circles, respectively], 50 pairings induced a robust LTP of PSPs (percentage of peak amplitude: from 96.01 ± 4.12% basal to 137.62 ± 8.33% at 40 min after 50 pairings; Fig. 5A, white triangles, ***p = 0.0007). We then performed similar experiments but in slices in which IGF-I had been bath applied previously. We allowed 25 min of IGF-I washout and a pyramidal neuron was “patched” and the number of pairings required to induce LTPH by STDP was tested. In these experiments, repeating 10 pairings was unable to induce the LTPH of the PSPs (percentage of peak amplitude: from 99.39 ± 1.46% basal to 99.79 ± 3.88% at 40 min after 10 pairings; p = 0.9271; Fig. 5B, red squares). However, 20 and 50 pairings induced a robust LTPH of PSPs [percentage of peak amplitude: from 99.36 ± 1.08% to 141.59 ± 4.38% (***p = 0.00008) basal; from 99.36 ± 0.89% to 169.13 ± 6.36% (***p = 0.0001) at 40 min after 20 and 50 pairings, respectively; Fig. 5B, red circles, white triangles). These results suggest that these pyramidal neurons had been facilitated by IGF-I, showing a reduction of the induction threshold of the LTPH (Fig. 5C).

Figure 5.
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Figure 5.

IGF-I favors Hebbian LTP ex vivo and in vivo. A, Top, Representative PSP followed by an AP with a 10 ms delay and a frequency of 0.2 Hz. Bottom, top, Representative PSPs in the control and 40 min after 10, 20, and 50 pairings for the STDP induction. Bottom, bottom, Time course of the PSPs with 10 pairings (n = 5 cells/2 animals basal vs after 40 min of STDP, ns), with 20 pairings (n = 6 cells/3 animals; basal vs after 40 min of STDP, ns), and with 50 pairings (n = 7 cells/3 animals; basal vs after 40 min of STDP, ***p < 0.001) for the STDP induction (black arrow). Two-tailed Student's paired t test. B, Top, Time course scheme showing the IGF-I exposure, washout, and STDP induction (black arrow). Bottom, top, Representative PSPs in the control and 40 min after 10, 20, and 50 pairings for the STDP induction after IGF-I exposure and washout. Bottom, bottom, Time course of the PSPs with 10 pairings (n = 5 cells/2 animals; basal vs after IGF-I, ns), 20 pairings (n = 6 cells/2 animals; basal vs after IGF-I, ***p < 0.001), and 50 pairings (n = 6 cells/3 animals; basal vs after IGF-I, ***p < 0.001) for the STDP induction (black arrow) after IGF-I exposure and washout. Two-tailed Student's paired t test. C, Cartoon showing that IGF-I exposure induces an LTP of the PSPs (LTPIGFI) that increases the magnitude of LTP by STDP and facilitates its induction. D, Top, Scheme showing the time course of local injection (arrows) and whisker stimulation [5 Hz, 10 stimuli (stim.), blue arrow] used in the in vivo experiments. Bottom, Drawing to illustrate the recording electrode and Hamilton syringe location, and air puff stimulation in the anesthetized mouse. E, Top, Representative LFPs in the control [black, (1)], after 80 min IGF-I and saline injections in the wild-type mouse [dark gray, (2)], and after 5 Hz train whisker stimulation [gray, (3)]. Bottom, Time course of LFP area with IGF-I injection (arrows) in the wild-type mouse (red triangle; n = 10 animals; basal vs after 80 min injection, ##p < 0.01; basal vs after 5 Hz train, ###p < 0.001; after 80 min injection vs after 5 Hz train, ##p < 0.01; F(2,26) = 20.65), previously intraperitoneally injected SCH (2 mg/kg; purple square; n = 7 animals; basal vs after 80 min injection, ns; basal vs after 5 Hz train, ns; after 80 min injection vs after 5HZ train, ns; nonparametric test; H = 1.983), IGF-IR−/− (green diamond; n = 6 animals; basal vs after 80 min injection, ns; basal vs after 5 Hz train, ns; after 80 min injection vs after 5HZ train, ns; nonparametric test; H = 0.3702), IGF-IR+/+ (dark green triangle; n = 7 animals; basal vs after 80 min injection, ##p < 0.01; basal vs after 5 Hz train, ###p < 0.001; after 80 min injection vs after 5 Hz train, ##p < 0.01; F(2,18) = 27.94), and saline injection (black circle; n = 9 animals; basal vs after 80 min injection, ns; basal vs after 5 Hz train, ns; after 80 min injection vs after 5 Hz train, ns; F(2,23) = 1.766). One-way ANOVA. F, Scheme showing the time course of the saline local injection (arrows) and the following 5 Hz train whisker stimulations. G, Time course of LFP area with IGF-I injection (arrows) in the wild-type mice with local saline injection (black arrows) and 10 (yellow arrow) and 30 (blue arrow) stimuli trains whisker stimulation at 5 Hz. Note that 30 stimuli induced the LTP of the LFP but 10 stimuli did not change the LFP amplitude (n = 7 animals; 10 stimuli train vs 30 stimuli train, *p < 0.05). H, LFP amplitude in the control, 80 min after injections, and after 5 Hz train whisker stimulation in different experimental conditions: the wild-type mice with IGF-I injection (red; n = 10 animals; basal vs after 80 min injection, ##p < 0.01; basal vs after 5 Hz train, ###p < 0.001; after 80 min injection vs after 5 Hz train, #p < 0.05; F(2,24) =18.97), saline injection (gray; n = 9 animals; basal vs after 80 min injection, ns; basal vs after 5 Hz train, ns; after 80 min injection vs after 5 Hz train, ns; F(2,24) = 0.4747), previous intraperitoneally injected SCH (purple; n = 7 animals; basal vs after 80 min injection, ns; basal vs after 5 Hz train, ns; after 80 min injection vs after 5 Hz train, ns; nonparametric test; H = 1.967), and the IGF-IR−/− mice (green; n = 6 animals; basal vs after 80 min injection, ns; basal vs after 5 Hz train, ns; after 80 min injection vs after 5 Hz train, ns; nonparametric test, H = 0.3702), and its littermates the IGF-IR+/+ mice (dark green; n = 7 animals; basal vs after 80 min injection, ##p < 0.01; basal vs after 5 Hz train, ###p < 0.001; after 80 min injection vs after 5 Hz train, ##p < 0.01; F(2,18) = 23.35, one-way ANOVA).

Repetitive whisker stimulation at the frequency used to explore the environment (5 Hz) induces a long-lasting increase in synaptic efficacy at layer II/III neurons depending on the activation of NMDA receptors (Barros-Zulaica et al., 2014). To test for a similar reduction in the LTPH induction threshold in vivo, we analyzed the effect of IGFI on this NMDAR-dependent LTP induced by repetitive stimulation of the vibrissae (Fig. 5D). In the control conditions using saline injections, 30 stimuli of the vibrissae at 5 Hz stimulation induced an LTP of the LFP (150.83 ± 12.20% of LFP area at baseline; Fig. 5F,G, *p = 0.0248), whereas 10 stimuli at 5 Hz train was not sufficient to modulate the LFP (percentage of LFP area: from 99.94 ± 0.33% basal to 101.76 ± 8.01% at 30 min after 5 Hz train (p = 0.5055); percentage of LFP amplitude: from 100.16 ± 0.57 basal to 99.29 ± 3.34% (p = 0.8699) at 30 min after 5 Hz train; Fig. 5E,G). However, 10 stimuli at 5 Hz induced an LTP of the LFP [percentage of LFP area: from 99.45 ± 1.80% basal to 201.66 ± 23.49% (###p < 0.0001) at 30 min after 5 Hz train; percentage of LFP amplitude: from 99.41 ± 0.55% basal to 223.53 ± 22.56% (###p < 0.0001) at 30 min after 5 Hz train; Fig. 5E,H, IGF-I] in mice in which LTPIGFI was previously induced by intracranial IGF-I injection (see above; Fig. 1C,D). This reduction in the threshold of induction of LTP was abolished when IGF-I was applied to mice in which the A2A adenosine receptor antagonist SCH had previously been intraperitoneally injected [percentage of LFP area: from 92.82 ± 1.21% basal to 96.74 ± 10.34% (p = 0.6545) at 30 min after 5 Hz train; percentage of LFP amplitude: from 97.61 ± 2.39% basal to 106.62 ± 9.12% (p = 0.5076) at 30 min after 5 Hz train; Fig. 5E,H, SCH+IGF-I] or when IGF-I was applied to the mice in which IGF-IR had been removed specifically from astrocytes [percentage of LFP area: from 101.51 ± 2.05% basal to 91.48 ± 11.85% (p = 0.9999) at 30 min after 5 Hz train; percentage of LFP amplitude: from 99.68 ± 0.31% basal to 106.40 ± 10.15% (p = 0.8977) at 30 min after 5 Hz train in IGF-IR−/− mice]. Interestingly, we observed an LTP of the LFP [percentage of LFP area: from 99.64 ± 0.43% basal to 241.08 ± 15.07% (***p < 0.0001) at 30 min after 5 Hz train; percentage of LFP amplitude: from 98.89 ± 1.02% basal to 206.60 ± 17.31% (***p < 0.0001) at 30 min after 5 Hz train in IGF-IR+/+ mice; Fig. 5E,H, IGF-IR−/−, IGF-IR+/+]. Therefore, these results indicate that IGF-I favors the induction of a LTP by reducing the threshold of its induction both ex vivo and in vivo (Fig. 6).

Figure 6.
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Figure 6.

Model showing the actions of IGF-I. Schematic diagram showing, from bottom to the top, that the activation of layer II/III astrocytes (1; orange cell in the bottom) by IGF-I (red circles) promotes the release of ATP/Ado (2). Ado acts on both the excitatory (3; blue) and inhibitory (3; gray) synapses inducing STP of the EPSCs (4), and LTD of the IPSCs (5), respectively (black and red superimposed responses are those recorded before and during the application of IGF-I). The net effect of this synaptic modulation is the LTPIGFI, an LTP of the PSP recorded from the soma of the layer II/III pyramidal neuron. The LTPIGFI facilitates the induction of the Hebbian LTP by STDP protocols and increases its magnitude.

The performance of a whisker discrimination task is impaired in the astrocyte-specific IGF-IR−/− mice

Finally, we tested whether the activation of IGF-IR on astrocytes is involved in the performance of a dependent discrimination task. We used a test based on the ability of the mice to discriminate different textures in the arms of a Y-maze (Fig. 6B). We compared the ability to perform this task on the astrocyte-specific IGF-IR−/− mice (Fig. 7A, ##p < 0.01) with their control littermates (IGF-I+/+ mice). No differences were observed between the IGF-IR−/− mice and their control littermates in the number of visits to the arms (p = 0.1142; Fig. 7B), indicating normal deambulatory activity. Interestingly, the IGF-IR−/− mice spent less time examining the arm with the novel texture (Fig. 7C, left, #p = 0.0336), indicating impaired texture discrimination (Fig. 7C, right, ##p = 0.0059). Conversely, whisker perception in the IGF-IR−/− mice was preserved, as indicated by their normal performance in the gap-crossing test (p = 0.6199; Fig. 7D, left). In addition, the working memory was also normal in the IGF-IR−/− mice, as indicated by preserved performance in the Y-maze alternation test (p = 0.7474; Fig. 7D, right). Together, these results indicate that the activation of IGF-IR on astrocytes increases the performance of the texture discrimination in mice, which suggests a crucial role in the learning and memory processes dependent on the barrel cortex activity.

Figure 7.
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Figure 7.

The performance of a whisker-based discrimination task is impaired in the astrocyte-specific IGF-IR−/− mice. A, Left, Schema of the transgenic mice generation with tamoxifen-regulated deletion of IGF-IR in astrocytes (IGF-IR−/− mice). Right, The brain levels of IGF-IR mRNA, as determined by quantitative PCR, are reduced in the IGF-IR−/− mice after tamoxifen injection, when compared with the vehicle-injected control mice (n = 5 animals injected with tamoxifen vs control, **p < 0.01). B, Left, Schema of Y-maze whisker-dependent texture discrimination test. Two arms (familiar) were covered with 500 grit sandpaper, and the third (novel) was covered with 220 grit sandpaper. Because the three arms of the maze are identical and there are no extramaze cues, discrimination of novelty versus familiarity relies only on the different textures that the mice can perceive with their whiskers. Right, IGF-IR−/− and the control mice show no difference in their entrance to the different arms (n = 13 animals; tamoxifen vs control, ns; two-way ANOVA with post hoc Bonferroni multiple-comparisons test. C, Left, However, the control mice spent more time exploring the novel arm (220 grit sandpaper) than the IGF-IR−/− mice (n = 13 animals; tamoxifen vs control, *p < 0.05). Right, The percentage of time spent in the novel-texture arm was higher in the controls than in the IGF-IR−/− mice (n = 13 animals; tamoxifen vs control, **p < 0.01, two-way ANOVA with post hoc Bonferroni multiple-comparisons test). D, Left, The control and the IGF-IR−/− mice showed a similar performance in the gap-crossing test, indicating that there were no alterations in their sensory perception (n = 5; tamoxifen vs control, ns). Right, No differences were observed between the control and the IGF-IR−/− mice in the Y-maze spontaneous alternation task, indicating a similar working memory (n = 5; tamoxifen vs control, ns; two-way ANOVA with post hoc Bonferroni multiple-comparisons test).

Discussion

The data in this study challenge the standard view that astrocytes play a key role in the induction of LTP or LTD, mainly on excitatory synapses in cortical circuits, and expand the view of the IGF-I action in the brain. Instead, we now provide conclusive evidence that astrocytes are mandatory intermediates for the LTD of inhibitory synapses in the barrel cortex, which relies on astrocytic release of ATP as a result of the IGF-IR activation. Although the observation that IGF-I action on the brain is crucial for learning and memory is well known, here we are proposing a reinterpretation of the classical IGF-I mechanism, from the IGF-I activation of neurons to its action on astrocytes. Therefore, the results presented in this study support the initial hypothesis that astrocytic IGF-IR is a key player in the IGF-I action related to learning and memory.

The inhibitory transmission controls the operation of cortical circuits, and the modulation of synaptic inhibition plays an important role in the induction of cortical plasticity. Our results show that IGF-I induces a long-lasting depression of the IPSCs that would result in an LTP of the PSPs that we have termed LTPIGFI. Moreover, we further analyzed how IGF-IR activation is able to favor the induction of NMDAR-dependent LTP and improves the performance of the texture discrimination in mice. We have shown that the activation of IGF-IRs in astrocytes triggers the calcium-dependent release of ATP/Ado, and the activation of A2A adenosine receptors results in the LTDIGFI by decreasing the probability of GABA release at the inhibitory terminals (Fig. 2B, mechanistic diagram). In addition, the ATP/Ado also induced a short-term increase in the efficacy of the excitatory synaptic transmission. The lack of LTDIGFI in mice in which IGF-IR has been selectively ablated in astrocytes suggests that IGF-IR activation in astrocytes is crucial to the induction of this novel form of long-lasting downregulation of cortical synaptic inhibition.

IGF-I elicits a long-lasting depression of GABA release by cerebellar Purkinje cells in response to glutamate, indicating that IGF-I may act as a modulator of glutamatergic transmission in the adult rat olivocerebellar system (Castro-Alamancos and Torres-Aleman, 1993; Castro-Alamancos et al., 1996). Also, IGF-I has been shown to modulate GABAergic transmission in the olfactory bulb (Liu et al., 2017). However, this is the first time that an IGF-I-mediated long-lasting depression of fast GABAergic synaptic transmission in the neocortex been described. We have demonstrated that IGF-I induces the release of ATP from cortical astrocytes that generates the long-lasting depression of GABA release. Although IGF-IRs may be present in the inhibitory GABAergic terminals, there are no clear data showing a direct action of IGF-I acting on presynaptic IGF-IRs. The only evidence suggesting this mode of action is seen in the hippocampus where IGF-I, possibly acting via GABAergic neurons, can induce the release of GABA to regulate endogenous ACh release (Seto et al., 2002). Nevertheless, our observations contribute to the notion that IGF-I can modulate both excitatory and inhibitory synaptic activity throughout the CNS.

Although there is evidence showing that insulin signaling in astrocytes mediates tyrosine phosphorylation of Munc18cand syntaxin-4-dependent ATP exocytosis, which in turn modulates presynaptic dopamine release (Cai et al., 2018), we have not found any previous evidence for any stimulatory action of IGF-I on ATP release by astrocytes. In this regard, it has been shown previously that metabotropic protease-activated receptor 1 (PAR-1) induces exocytosis of ATP from cortical astrocytes, which leads to a short-term downregulation of inhibitory synaptic currents in layer II/III pyramidal neurons (Lalo et al., 2014). In contrast with the presynaptic LTD described herein, this short-term modulation is mediated by a postsynaptic mechanism in which Ca2+ entry through the neuronal P2X purine receptor leads to a phosphorylation-dependent downregulation of GABAA receptors. Therefore, astrocytes can downregulate or upregulate inhibitory synaptic transmission by a calcium-dependent release of ATP depending on whether IGF-IR or PAR-1 receptor, respectively, is activated.

Astrocytes have been shown to respond to both glutamate and GABA (Pasti et al., 1997; Kang et al., 1998; Guthrie et al., 1999; Navarrete and Araque, 2008, 2010), which allows them to sense the activity of excitatory and inhibitory neurons. In response to these neurotransmitters, they can release both glutamate and ATP/Ado (Min et al., 2012; Araque et al., 2014; De Pittà et al., 2016; Guerra-Gomes et al., 2018; Savtchouk and Volterra, 2018). In fact, hippocampal interneuron activity leads to GABAB receptor-mediated release of glutamate from astrocytes that potentiates both inhibitory (Kang et al., 1998) and excitatory (Perea et al., 2016) synaptic transmission. In addition to glutamate, hippocampal astrocytes may also release ATP, which is converted to adenosine that depresses (Pascual et al., 2005; Serrano et al., 2006; Andersson et al., 2007; Chen et al., 2013) or potentiates excitatory synaptic transmission (Panatier et al., 2011). Moreover, ATP released by astrocytes can depress excitatory synapses from basolateral amygdala and enhance inhibitory synapses from the lateral subdivision of the central amygdala via the activation of A1 and A2A adenosine receptors, respectively (Martin-Fernandez et al., 2017). Furthermore, cortical astrocytes have been shown to induce a short-term depression of inhibitory synaptic currents (Lalo et al., 2014). However, to our knowledge, our research is the first evidence that IGF-IR activation in astrocytes can induce a long-lasting depression of inhibitory cortical synaptic transmission through the release of ATP/Ado via a presynaptic mechanism. Therefore, we are presenting novel data showing that not only does the astrocyte-mediated LTPIGFI and its physiological consequence favor the associative synaptic plasticity described by Donald Hebbs, but also a novel mechanism in which IGF-I would induce cortical plasticity through the activation of adenosine receptors.

At the circuit level, interneurons control the flow of information and synchronization in the cerebral cortex. Synaptic inhibition is involved in the emergence of fast brain rhythms (Csicsvari et al., 2003) and in the induction of synaptic plasticity (Hensch, 2005), which jointly contribute to cognitive functions. Indeed, disruption of astrocytic vesicular release has been found to be crucial for gamma oscillatory hippocampal activity with a significant impact on recognition memory tasks (Lee et al., 2014). On the other hand, cortical astrocytes respond to sensory inputs and control sensory-evoked cortical neuronal network activity, indicating that astrocytes are actively involved in cortical sensory information processing (Lines et al., 2020). Our results demonstrate that the IGF-I receptor on astrocytes improves the performance of texture discrimination in mice, suggesting that the LTDIGFI is essential in this dependent task. The release of ATP/Ado from astrocytes and the LTDIGFI described here play a relevant role in this cognitive function by controlling brain rhythms and favoring the induction of Hebbian synaptic plasticity. Decreased synaptic inhibition would facilitate the backpropagation of action potentials into the dendrites and the induction of spike timing-dependent plasticity. Furthermore, by changing the ratio between synaptic excitation and inhibition, neuronal membranes can rapidly reach the threshold for action potential generation, and enhanced cortical activity is expected when cortical levels of IGF-I increase. Indeed, this increase in cortical activity would activate the neurotrophic coupling mechanism for the entrance of IGF-I from the plasma into the brain (Nishijima et al., 2010).

IGF-I is able to enhance glutamatergic synaptic transmission in the hippocampal slices of juvenile rats through a mechanism that involves AMPA, but not NMDA, postsynaptic receptors (Ramsey et al., 2005). However, IGF-I significantly increases both AMPA- and NMDA-mediated synaptic transmission by a postsynaptic mechanism in young adults and mature rats (Molina et al., 2012). Additionally, the increase in the expression levels of the NMDA receptor subunits 2A and 2B at the hippocampus by IGF-I in aged rats (Sonntag et al., 2000) may facilitate LTP induction. As in the hippocampus, we have shown that IGF-I increases the efficacy of excitatory synaptic transmission. The short-term potentiation of the EPSCs by IGF-I would establish a temporal window in which the threshold for inducing the Hebbian LTP would be the lowest because of the modulation of both excitation and inhibition by IGF-I. According to this scenario, another question arises as to how the IGF-IR activation on astrocytes is linked with the release of ATP. However, these questions should be the subject of analyses of further details in future articles.

In summary, our findings reveal novel mechanisms and functional consequences of IGF-I signaling in the cortex. It induces the long-lasting depression of inhibition and short enhancement of the excitation opening a temporal window to favor the generation of associative memories that impact on the behavioral performance of barrel cortex-related texture discrimination tasks, through the activation of cortical astrocytes.

Footnotes

  • This work was supported by Grants BFU2016-80802-P from Agencia Estatal de Investigación Spain/Fondo Europeo de Desarrollo Regional, and from the European Union [Ministerio de Economía y Competitividad (MINECO)] to D.F.d.S.; Grants R01-NS-097312 and R01-DA-048822 from National Institutes of Health/National Institute of Neurological Disorders and Stroke to A.A.; and grants from Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED) and Grant SAF2016-76462-C2-1-P from MINECO to I.T.-A. J.A.Z.-V. was supported by the National Council of Science, Technology and Technological Innovation (CONCYTEC, Perú) through the National Fund for Scientific and Technological Development (FONDECYT, Perú). J.F. received a postdoctoral fellowship from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP; Grants #2017/14742-0 and #2019/03368-5). We thank the University of Minnesota Viral Vector and Cloning Core for production of some of the viral vectors used in this study; and Dr. G. Perea and Dr. Washington Buño for helpful comments.

  • The authors declare no competing financial interests.

  • Correspondence should be a addressed to David Fernández de Sevilla at david.fernandezdesevilla{at}uam.es

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Journal of Neuroscience
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2 Jun 2021
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Astrocytic IGF-IRs Induce Adenosine-Mediated Inhibitory Downregulation and Improve Sensory Discrimination
José Antonio Noriega-Prieto, Laura Eva Maglio, Jonathan A. Zegarra-Valdivia, Jaime Pignatelli, Ana M. Fernandez, Laura Martinez-Rachadell, Jansen Fernandes, Ángel Núñez, Alfonso Araque, Ignacio Torres-Alemán, David Fernández de Sevilla
Journal of Neuroscience 2 June 2021, 41 (22) 4768-4781; DOI: 10.1523/JNEUROSCI.0005-21.2021

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Astrocytic IGF-IRs Induce Adenosine-Mediated Inhibitory Downregulation and Improve Sensory Discrimination
José Antonio Noriega-Prieto, Laura Eva Maglio, Jonathan A. Zegarra-Valdivia, Jaime Pignatelli, Ana M. Fernandez, Laura Martinez-Rachadell, Jansen Fernandes, Ángel Núñez, Alfonso Araque, Ignacio Torres-Alemán, David Fernández de Sevilla
Journal of Neuroscience 2 June 2021, 41 (22) 4768-4781; DOI: 10.1523/JNEUROSCI.0005-21.2021
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Keywords

  • astrocytes
  • barrel cortex
  • IGF-I
  • long-term depression
  • long-term potentiation
  • sensory discrimination

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