Synaptic morphology | | | | |
n | 45 | 45 | | |
Number of SV2 clusters (puncta/100 µm²) | 41.55 ± 2.6 | 41.76 ± 2.96 | 0.96 | |
Area of SV2 clusters (µm²) | 7.98 ± 0.58 | 7.16 ± 0.56 | 0.32 | |
Relative SV2 immunoreactivity | 3.56 ± 0.11 | 3.54 ± 0.13 | 0.92 | |
AZ composition | | | | |
n | 45 | 45 | | |
Number Bassoon clusters (particles/100 µm²) | Mir-183/96 wt: 38.19 ± 2.29 | Mir-183/96dko: 32.44 ± 2.10 | 0.067 | |
| Cldn14wt: 40.28 ± 2.30 | Cldn14ko: 34.37 ± 2.75 | 0.11 | |
Size Bassoon clusters (µm²) | Mir-183/96 wt: 0.046 ± 0.0024 | Mir-183/96dko 0.056 ± 0.0022 | 0.0022 | 0.683 |
| Cldn14wt: 0.044 ± 0.0014 | Cldn14ko: 0.048 ± 0.0019 | 0.26 | |
| | Mir-183/96dko vs. Cldn14ko | 0.0238 | 0.555 |
Relative Bassoon immunoreactivity | Mir-183/96 wt: 4.85 ± 0.17 | Mir-183/96dko: 4.89 ± 0.15 | 0.85 | |
| Cldn14wt: 5.50 ± 0.15 | Cldn14ko: 5.40 ± 0.21 | 0.73 | |
Number Piccolo clusters (particles/100 µm²) | Mir-183/96 wt: 28.81 ± 1.83 | Mir-183/96dko: 27.45 ± 1.67 | 0.56 | |
| Cldn14wt: 37.18 ± 2.95 | Cldn14ko: 36.06 ± 2.03 | 0.77 | |
Size Piccolo clusters (µm²) | Mir-183/96 wt: 0.062 ± 0.0025 | Mir-183/96dko: 0.080 ± 0.0028 | <0.0001 | 1.023 |
| Cldn14wt: 0.054 ± 0.0016 | Cldn14ko: 0.061 ± 0.0020 | 0.13 | |
| | Mir-183/96dko vs. Cldn14ko | <0.0001 | 1.173 |
Relative Piccolo immunoreactivity | Mir-183/96 wt: 4.13 ± 0.11 | Mir-183/96dko: 4.63 ± 0.12 | 0.02 | 0.693 |
| Cldn14wt: 4.39 ± 0.13 | Cldn14ko: 4.27 ± 0.16 | 0.57 | |
AZ ultrastructure | | | | |
n | 36–41 | 31–25 | 0.349 | |
AZ length (nm) | 343 ± 10 | 358 ± 12 | 0.586 | |
SV diameter (nm) | 38 ± 0.4 | 38 ± 0.7 | 0.0029 | 0.809 |
Rel. fraction SVs within 20 nm | 0.058 ± 0.004 | 0.089 ± 0.008 | 0.012 | 0.675 |
Number SVs within 20 nm/AZ | 0.766 ± 0.06 | 1.012 ± 0.074 | | |
GluA1 puncta | | | | |
n | 45 | 45 | | |
Number (puncta/100 µm²) | Mir-183/96 wt: 15.15 ± 1.19 | Mir-183/96dko: 27.78 ± 1.39 | <0.0001 | −3.92 |
| Cldn14wt: 6.92 ± 0.86 | Cldn14ko: 10.89 ± 0.98 | 0.12 | |
| | Mir-183/96dko vs. Cldn14ko | <0.0001 | 2.741 |
Size (µm²) | Mir-183/96 wt: 0.026 ± 0.00084 | Mir-183/96dko: 0.041 ± 0.0024 | <0.0001 | 1.263 |
| Cldn14wt: 0.024 ± 0.00073 | Cldn14ko: 0.021 ± 0.00074 | 0.59 | |
| | Mir-183/96dko vs. Cldn14ko | <0.0001 | 1.572 |
Homer puncta | | | | |
n | 30 | 30 | | |
cumulative Homer1 area (µm²) | 31.11 ± 3.03 | 47.67 ± 2.71 | <0.0001 | −1.061 |
Synaptic GluA1 | | | | |
n | 30–45 | 30–45 | | |
Co-localization with Homer1 (puncta/Homer area) | 0.439 ± 0.053 | 0.939 ± 0.131 | 0.0009 | −0.919 |
Co-localization with Piccolo (puncta/100 µm²) | Mir-183/96 wt: 13.82 ± 1.31 | Mir-183/96dko: 21.61 ± 1.52 | 0.0017 | 0.8 |
| Cldn14wt: 20.08 ± 1.76 | Cldn14ko: 24.21 ± 1.67 | 0.19 | |