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Research Articles, Systems/Circuits

High-Frequency Neuronal Bursting is Essential for Circadian and Sleep Behaviors in Drosophila

Florencia Fernandez-Chiappe, Lia Frenkel, Carina Celeste Colque, Ana Ricciuti, Bryan Hahm, Karina Cerredo, Nara Inés Muraro and María Fernanda Ceriani
Journal of Neuroscience 27 January 2021, 41 (4) 689-710; https://doi.org/10.1523/JNEUROSCI.2322-20.2020
Florencia Fernandez-Chiappe
1Instituto de Investigación en Biomedicina de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Partner Institute of the Max Planck Society, Buenos Aires C1425FQD, Argentina
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Lia Frenkel
2Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires C1405BWE, Argentina
3Instituto de Biociencias, Biotecnología y Biología traslacional (IB3), Departamento de Fisiología Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires C1428EGA, Argentina
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Carina Celeste Colque
2Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires C1405BWE, Argentina
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Ana Ricciuti
1Instituto de Investigación en Biomedicina de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Partner Institute of the Max Planck Society, Buenos Aires C1425FQD, Argentina
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Bryan Hahm
1Instituto de Investigación en Biomedicina de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Partner Institute of the Max Planck Society, Buenos Aires C1425FQD, Argentina
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Karina Cerredo
1Instituto de Investigación en Biomedicina de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Partner Institute of the Max Planck Society, Buenos Aires C1425FQD, Argentina
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Nara Inés Muraro
1Instituto de Investigación en Biomedicina de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Partner Institute of the Max Planck Society, Buenos Aires C1425FQD, Argentina
2Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires C1405BWE, Argentina
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María Fernanda Ceriani
2Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires C1405BWE, Argentina
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Article Information

DOI 
https://doi.org/10.1523/JNEUROSCI.2322-20.2020
PubMed 
33262246
Published By 
Society for Neuroscience
History 
  • Received September 3, 2020
  • Revision received November 4, 2020
  • Accepted November 9, 2020
  • First published December 1, 2020.
  • Version of record published January 27, 2021.
Copyright & Usage 
Copyright © 2021 the authors SfN exclusive license.

Author Information

  1. Florencia Fernandez-Chiappe1,*,
  2. Lia Frenkel2,3,*,
  3. Carina Celeste Colque2,
  4. Ana Ricciuti1,
  5. Bryan Hahm1,
  6. Karina Cerredo1,
  7. Nara Inés Muraro1,2, and
  8. María Fernanda Ceriani2
  1. 1Instituto de Investigación en Biomedicina de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Partner Institute of the Max Planck Society, Buenos Aires C1425FQD, Argentina
  2. 2Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires C1405BWE, Argentina
  3. 3Instituto de Biociencias, Biotecnología y Biología traslacional (IB3), Departamento de Fisiología Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires C1428EGA, Argentina
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Author contributions

  1. Author contributions: N.I.M. and M.F.C. designed research; F.F.-C., L.F., C.C.C., A.R., B.H., K.C., and N.I.M. performed research; F.F.-C., L.F., C.C.C., A.R., B.H., K.C., and N.I.M. analyzed data; N.I.M. and M.F.C. wrote the paper.

  2. ↵*F.F.-C. and L.F. contributed equally to this work.

Disclosures

    • Received September 3, 2020.
    • Revision received November 4, 2020.
    • Accepted November 9, 2020.
  • This work was supported by Agencia Nacional de Promoción Científica y Tecnológica Grants PICT-2011-2185 (to M.F.C.) and PICT-2011-2364 and PICT-2015-2557 (to N.I.M.), the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) Grant PIP-11220130100378 (to N.I.M.) and the Fondo de Convergencia Estructural del MERCOSUR Grant COF 03/11 to Instituto de Investigación en Biomedicina de Buenos Aires (IBioBA). M.F.C., N.I.M., and L.F. are members of the CONICET. Stocks obtained from the Bloomington Drosophila Stock Center (National Institutes of Health Grant P40OD018537), the NIG-Fly Stock Center, and the Vienna Drosophila Resource Center (Dietzl et al., 2007) were used in this study. We thank Dr. Zuo Ren Wang for Ih mutant fly stocks. We also thank Esteban Beckwith and Quentin Geissmann for indispensable help with sleep quantification software. We thank Andres Liceri for fly maintenance.

  • The authors declare no competing financial interests.

  • Correspondence should be addressed to Nara Inés Muraro at nmuraro{at}ibioba-mpsp-conicet.gov.ar or María Fernanda Ceriani at fceriani{at}leloir.org.ar

Funding

  • MINCyT | ANPCyT | Fondo para la Investigación Científica y Tecnológica (FonCyT)

    PICT-2011-2185; PICT-2011-2364; PICT-2015-2557
  • Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)

    PIP-11220130100378

Other Version

  • previous version (December 01, 2020).
  • You are viewing the most recent version of this article.

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Total411126481325
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The Journal of Neuroscience: 41 (4)
Journal of Neuroscience
Vol. 41, Issue 4
27 Jan 2021
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High-Frequency Neuronal Bursting is Essential for Circadian and Sleep Behaviors in Drosophila
Florencia Fernandez-Chiappe, Lia Frenkel, Carina Celeste Colque, Ana Ricciuti, Bryan Hahm, Karina Cerredo, Nara Inés Muraro, María Fernanda Ceriani
Journal of Neuroscience 27 January 2021, 41 (4) 689-710; DOI: 10.1523/JNEUROSCI.2322-20.2020

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High-Frequency Neuronal Bursting is Essential for Circadian and Sleep Behaviors in Drosophila
Florencia Fernandez-Chiappe, Lia Frenkel, Carina Celeste Colque, Ana Ricciuti, Bryan Hahm, Karina Cerredo, Nara Inés Muraro, María Fernanda Ceriani
Journal of Neuroscience 27 January 2021, 41 (4) 689-710; DOI: 10.1523/JNEUROSCI.2322-20.2020
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Keywords

  • bursting neuron
  • Drosophila melanogaster
  • HCN
  • Ih
  • ion channel
  • pigment dispersing factor

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