Figure 1. Zcchc12 genetically represented a subpopulation of Gal+ DRG neurons. A, ISH showed the expression of Zcchc12 in a lumbar DRG. Histogram represents the size distribution of Zcchc12+ DRG neurons. Scale bar, 50 μm. B, Histogram represents the size distribution of Zcchc12+, Cldn9+, Gal+, Nppb+, Th+, Mrgpra3+, and Mrgprd+ DRG neurons. C, RNAscope ISH showed the localization of the Zcchc12 with Gal, Cldn9, Nppb, Vglut3, Mrgpra3, Mrgprd, and S100b in the lumbar DRG of the WT C57 mouse. Scale bar, 50 μm. D-F, RNAscope ISH showed coexpression of Zcchc12 with Tac1, Calca, and Nppb. Scale bar, 50 μm. G, Histogram represents the percentage of the colocalization with Zcchc12+ neurons. H-J, Expression of tdTomato in the lumbar DRG, spinal cord, and brain of the Zcchc12-CreERT2::Ai9 transgenic mouse. Scale bar, 100 μm. K, RNAscope ISH showed the expression of tdTomato and Gal, Cldn9, Nppb, Vglut3, Mrgpra3, Mrgprd, and S100b in the lumbar DRG of Zcchc12-CreERT2::Ai9 transgenic mice. Scale bar, 50 μm.