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Research Articles, Behavioral/Cognitive

Nicotinic Acetylcholine Receptors Expressed by Striatal Interneurons Inhibit Striatal Activity and Control Striatal-Dependent Behaviors

Alice Abbondanza, Irina Ribeiro Bas, Martin Modrak, Martin Capek, Jessica Minich, Alexandra Tyshkevich, Shahed Naser, Revan Rangotis, Pavel Houdek, Alena Sumova, Sylvie Dumas, Veronique Bernard and Helena Janickova
Journal of Neuroscience 30 March 2022, 42 (13) 2786-2803; DOI: https://doi.org/10.1523/JNEUROSCI.1627-21.2022
Alice Abbondanza
1Laboratory of Neurochemistry, Institute of Physiology of the Czech Academy of Sciences, Prague, 14220, Czech Republic
6Neuroscience ParisSeine, Institut de Biologie Paris Seine, Institut National de la Santé et de la Recherche Médicale, Centre National de la Recherche Scientifique, Sorbonne Université, Paris, 75005, France
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Irina Ribeiro Bas
1Laboratory of Neurochemistry, Institute of Physiology of the Czech Academy of Sciences, Prague, 14220, Czech Republic
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Martin Modrak
4Bioinformatics Core Facility, Institute of Microbiology of the Czech Academy of Sciences, Prague, 14220, Czech Republic
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Martin Capek
3Laboratory of Biomathematics, Institute of Physiology of the Czech Academy of Sciences, Prague, 14220, Czech Republic
5Light Microscopy Core Facility, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, 14220, Czech Republic
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Jessica Minich
1Laboratory of Neurochemistry, Institute of Physiology of the Czech Academy of Sciences, Prague, 14220, Czech Republic
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Alexandra Tyshkevich
1Laboratory of Neurochemistry, Institute of Physiology of the Czech Academy of Sciences, Prague, 14220, Czech Republic
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Shahed Naser
1Laboratory of Neurochemistry, Institute of Physiology of the Czech Academy of Sciences, Prague, 14220, Czech Republic
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Revan Rangotis
1Laboratory of Neurochemistry, Institute of Physiology of the Czech Academy of Sciences, Prague, 14220, Czech Republic
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Pavel Houdek
2Laboratory of Biological Rhythms, Institute of Physiology of the Czech Academy of Sciences, Prague, 14220, Czech Republic
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Alena Sumova
2Laboratory of Biological Rhythms, Institute of Physiology of the Czech Academy of Sciences, Prague, 14220, Czech Republic
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Sylvie Dumas
7Oramacell, Paris, 75006, France
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Veronique Bernard
6Neuroscience ParisSeine, Institut de Biologie Paris Seine, Institut National de la Santé et de la Recherche Médicale, Centre National de la Recherche Scientifique, Sorbonne Université, Paris, 75005, France
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Helena Janickova
1Laboratory of Neurochemistry, Institute of Physiology of the Czech Academy of Sciences, Prague, 14220, Czech Republic
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Abstract

Acetylcholine is an important modulator of striatal activity, and it is vital to controlling striatal-dependent behaviors, including motor and cognitive functions. Despite this significance, the mechanisms determining how acetylcholine impacts striatal signaling are still not fully understood. In particular, little is known about the role of nAChRs expressed by striatal interneurons. In the present study, we used FISH to determine which neuronal types express the most prevalent beta2 nicotinic subunit in the mouse striatum. Our data support a common view that nAChR expression is mostly restricted to striatal interneurons. Surprisingly though, cholinergic interneurons were identified as a population with the highest expression of beta2 nicotinic subunit. To investigate the functional significance of beta2-containing nAChRs in striatal interneurons, we deleted them by injecting the AAV-Cre vector into the striatum of beta2-flox/flox male mice. The deletion led to alterations in several behavioral domains, namely, to an increased anxiety-like behavior, decrease in sociability ratio, deficit in discrimination learning, and increased amphetamine-induced hyperlocomotion and c-Fos expression in mice with beta2 deletion. Further colocalization analysis showed that the increased c-Fos expression was present in both medium spiny neurons and presumed striatal interneurons. The present study concludes that, despite being relatively rare, beta2-containing nAChRs are primarily expressed in striatal neurons by cholinergic interneurons and play a significant role in behavior.

SIGNIFICANCE STATEMENT A large variety of nAChRs are expressed in the striatum, a brain region that is crucial in the control of behavior. The complexity of receptors with different functions is hindering our understanding of mechanisms through which striatal acetylcholine modulates behavior. We focused on the role of a small population of beta2-containing nAChRs. We identified neuronal types expressing these receptors and determined their impact in the control of explorative behavior, anxiety-like behavior, learning, and sensitivity to stimulants. Additional experiments showed that these alterations were associated with an overall increased activity of striatal neurons. Thus, the small population of nicotinic receptors represents an interesting target for a modulation of response to stimulant drugs and other striatal-based behavior.

  • acetylcholine
  • behavior
  • Fos
  • interneurons
  • nAChRs
  • striatum

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The Journal of Neuroscience: 42 (13)
Journal of Neuroscience
Vol. 42, Issue 13
30 Mar 2022
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Nicotinic Acetylcholine Receptors Expressed by Striatal Interneurons Inhibit Striatal Activity and Control Striatal-Dependent Behaviors
Alice Abbondanza, Irina Ribeiro Bas, Martin Modrak, Martin Capek, Jessica Minich, Alexandra Tyshkevich, Shahed Naser, Revan Rangotis, Pavel Houdek, Alena Sumova, Sylvie Dumas, Veronique Bernard, Helena Janickova
Journal of Neuroscience 30 March 2022, 42 (13) 2786-2803; DOI: 10.1523/JNEUROSCI.1627-21.2022

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Nicotinic Acetylcholine Receptors Expressed by Striatal Interneurons Inhibit Striatal Activity and Control Striatal-Dependent Behaviors
Alice Abbondanza, Irina Ribeiro Bas, Martin Modrak, Martin Capek, Jessica Minich, Alexandra Tyshkevich, Shahed Naser, Revan Rangotis, Pavel Houdek, Alena Sumova, Sylvie Dumas, Veronique Bernard, Helena Janickova
Journal of Neuroscience 30 March 2022, 42 (13) 2786-2803; DOI: 10.1523/JNEUROSCI.1627-21.2022
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Keywords

  • acetylcholine
  • behavior
  • Fos
  • interneurons
  • nAChRs
  • striatum

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