Antibodies | | | |
Cathepsin B Cathepsin D DNER NSG2 NSG2 EEA1 LAMP1 Rab7 Rab7 MAP2 CI-M6PR GM130 p150glued DIC Myc 9E10 Flag M2 β3-tubulin Alexa-488 anti-mouse Alexa-488 anti-rabbit Alexa-488 anti-goat Alexa-568 anti-mouse Alexa-568 anti-rabbit Alexa-568 anti-goat Alexa-647 anti-mouse Alexa-647 anti-rabbit Alexa 647 anti-goat Dylight 405 anti-chicken Alexa 680 anti-mouse Alexa 790 anti-rabbit Alexa-680 anti-chicken Alexa-800 anti-goat | Goat Goat Goat Goat Rabbit Mouse Mouse Rabbit Rabbit Chicken Rabbit Mouse Mouse Mouse Mouse Mouse Chicken Donkey Donkey Donkey Donkey Donkey Donkey Donkey Donkey Donkey Donkey Donkey Donkey Donkey Donkey | R&D Systems AF965 R&D Systems AF1029 R&D Systems AF2254 Everest Biotech EB12967 Abcam ab189513 BD Biosciences 610456 Enzo Life Sciences ADI-VAM-EN001 Cell Signaling 9367 Abcam ab137029 EnCor Biotechnology CPCA-MAP2 Abcam ab124767 BD Biosciences 610822 BD Biosciences 612708 Santa Cruz Biotechnology sc-13524 Santa Cruz Biotechnology sc-40 Sigma, F3165 Aves Labs, TUJ Invitrogen A21202 Invitrogen A21206 Invitrogen A11055 Invitrogen A10037 Invitrogen A10042 Invitrogen A11057 Invitrogen A31571 Invitrogen A31573 Invitrogen A21447 Jackson ImmunoResearch Laboratories 703-475-155 Jackson ImmunoResearch Laboratories 715–625-151 Jackson ImmunoResearch Laboratories 711–655-162 Jackson ImmunoResearch Laboratories 703–625-155 LICOR Biosciences 926–32214 | AB_2086949 AB_2087094 AB_355202 AB_2571866 AB_399409 AB_2038958 AB_10693925 AB_2629474 AB_2138173 AB_10974087 AB_398141 AB_399947 AB_668849 AB_627268 AB_259529 AB_2313564 AB_141607 AB_2535792 AB_2534102 AB_2534013 AB_2534017 AB_2534104 AB_162546 AB_2536183 AB_2535864 AB_2340373 |
Plasmids | | | |
NSG1-mcherry EGFP-Rab7 EGFP-Rab7T22N mCherry-Rab7 GFP-RILP GFP-RILP199-401 GFP-RILP1-198 Myc-RILP GFP-p150-CC1 GFP-DIC2C FYCO1-Flag GFP mCherry siRNA-rat Rab7 siControl-nontargeting RNA | Yap et al., 2017 Addgene, #12605, Richard Pagano laboratory (Choudhury et al., 2002) Addgene, #12660, Richard Pagano laboratory (Choudhury et al., 2002) Gift from Michael Davidson laboratory Wang and Hong, 2006 Wang and Hong, 2006 Wang and Hong, 2006 Wang and Hong, 2002 Gift from Dr. Kevin Pfister Gift from Dr. Kevin Pfister Genscript clone ID: OHu21346 Clontech Clontech 5′-GACCAAGAACACACACGUA, Dharmacon #J-089334-10-0010 5′-UGGUUUACAUGUCGACUAA, Dharmacon #D-001810-01-05 | | |
Chemicals | | | |
LysoTracker Red DND99 DQRedBSA Ciliobrevin D CHX DMSO | Thermo Fisher Scientific #L7528 Thermo Fisher Scientific #D12051 Millipore Sigma #250401 Millipore Sigma #C7698 Millipore Sigma #D2650 | | |