Article Information
- Received December 12, 2021
- Revision received April 28, 2022
- Accepted May 31, 2022
- First published July 22, 2022.
- Version of record published August 31, 2022.
Author Information
- Carlo N. G. Giachello1,2,*,
- Iain Hunter1,2,*,
- Tom Pettini3,
- Bramwell Coulson1,2,
- Athene Knüfer3,
- Sebastian Cachero4,
- Michael Winding3,
- Aref Arzan Zarin5,
- Hiroshi Kohsaka6,
- Yuen Ngan Fan1,2,
- Akinao Nose7,
- Matthias Landgraf3, and
- Richard A. Baines1,2
- 1Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, United Kingdom
- 2Manchester Academic Health Science Centre, Manchester M13 9NQ, United Kingdom
- 3Department of Zoology, University of Cambridge, Cambridge CB2 3EJ, United Kingdom
- 4Neurobiology Division, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, United Kingdom
- 5Department of Biology, Texas A&M University, College Station, Texas 77843-3258
- 6Graduate School of Informatics and Engineering, The University of Electro-Communications, Tokyo 182-8585, Japan
- 7Department of Complexity Science and Engineering, Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8561, Japan
Author contributions
Author contributions: C.N.G.G., I.H., A.N., M.L., and R.A.B. designed research; C.N.G.G., I.H., T.P., A.K., H.K., Y.N.F., A.N., and M.L. performed research; B.C., S.C., M.W., A.A.Z., H.K., Y.N.F., A.N., M.L., A.A.Z., and R.A.B. contributed unpublished reagents/analytic tools; C.N.G.G., I.H., T.P., A.K., H.K., and Y.N.F. analyzed data; C.N.G.G. and I.H. wrote the paper.
↵*CN.G.G. and I.H. are joint first authors.
Disclosures
- Received December 12, 2021.
- Revision received April 28, 2022.
- Accepted May 31, 2022.
This work was supported by funding from the Biotechnology and Biological Sciences Research Council to R.A.B. (Grant BB/N/014561/1) and to M.L. (Grant BB/R016666/1) and by a Joint Wellcome Trust investigator award to R.A.B. and M.L. (Grant 217099/Z/19/Z). S.C. was supported by a Dorothy Hodgkin Fellowship from the Royal Society to S.C. (Grant DH120072), and benefited from core support from the MRC (Grant MC-U105188491). Work on this project benefited from the Manchester Fly Facility, established through funds from the University and the Wellcome Trust (Grant 087742/Z/08/Z); and from the Imaging Facility, Department of Zoology, University of Cambridge, which was supported by Dr. Matthew Wayland and funds from Wellcome Trust Equipment Grant WT079204 with contributions by the Sir Isaac Newton Trust in Cambridge, including Research Grant 18.07ii(c). We thank Chris Doe for generous provision of GAL4 lines and for advice in conducting this study, and Jan Felix Evers for designing a cyan fluorescent version of GRASP (GFP Reconstitution across Synaptic Partners).
The authors declare no competing financial interests.
- Correspondence should be addressed to Richard A. Baines at Richard.Baines{at}manchester.ac.uk
Funding
UKRI | Biotechnology and Biological Sciences Research Council (BBSRC)
BB/N/014561/1; BB/R016666/1Wellcome
217099/Z/19/Z; 087742/Z/08/Z; WT079204Royal Society (The Royal Society)
DH120072Sir Isaac Newton Trust
18.07ii(c)
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