Figure 5. RS1-deficient retinas display aberrant spontaneous glial signals after P15. A, B, Spontaneous glial ripples at GCL of control (A) and KO (B) retinal explants, for each age examined. The three panels at the bottom display still images at key temporal points of the glial ripple. C, Example fluorescence traces for both genotypes at key developmental stage. D, Frequency of spontaneous glial ripple per explant. E–G, Statistical metrics for glial events including velocity (E), duration (E), and distance (F) for a maximum five events per explant. N = 11 (P15 control), 8 (P15 KO), 10 (P20 control), 11 (P20 KO), 8 (P60 control), and 12 (P60 KO) from six mice per condition. D, After eye opening, using a Mann–Whitney test with multiple comparisons, the p-values at each age when comparing genotypes were 0.028 (P15; N = 11 WT explants and 15 KO explants), <0.0001 (P20; N = 10 control explants and 11 KO explants), and 0.0014 (P60; N = 8 control explants and 12 KO explants). Mann–Whitney tests yielded p-values of 0.005 (E, distance, P15), 0.058 (E, distance, P20), 0.1944 (E, distance, P60), 0.646 (F, duration, P15), 0.0151 (F, duration, P20), 0.710 (F, duration, P60), 0.332 (G, velocity, P15), 0.024 (G, velocity, P20), and 0.537 (G, velocity, P60). non-signifiant (ns) p > 0.05, * p ≤ 0.05, **≤0.01, ****≤0.0001.