Figure 4-1
OLIG2+ cell numbers and GFAP+ area are unchanged after PLX5622 administration. (A) Representative images of OLIG2 expression (red) in the cortex, corpus callosum, and striatum of MBP29 and WT mice fed with either PLX5622 or control diet for 12 weeks. Nuclei were visualized using DAPI (white). Dashed lines frame the corpus callosum. Scale bar: 20 μm. Quantification of OLIG2+/mm2 cells of MBP29 and WT mice fed with PLX5622 or control is indicated on the right. Data are mean ± SD (n = 10 animals per group, sex-matched). (B) Representative maximum intensity images of GFAP expression (cyan) and SOX2 (red) in the cortex, corpus callosum, and striatum of MBP29 and WT mice fed with either PLX5622 or control for 12 weeks. Nuclei were visualized using DAPI (white). Dashed lines frame the corpus callosum. Scale bar: 10 μm. Quantification of GFAP+ area of MBP29 and WT mice fed with PLX5622 or control is indicated on the right. Data are mean ± SD (n = 4 animals per group, sex-matched). Statistical analyses were performed using Shapiro–Wilk to test normal distribution and the Levene test for homogeneity of variance followed by Kruskal–Wallis test for pairwise comparison in combination with Dunn-Bonferroni post hoc method. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001. Download Figure 4-1, TIF file.