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Featured ArticleResearch Articles, Neurobiology of Disease

Loss of Activity-Induced Mitochondrial ATP Production Underlies the Synaptic Defects in a Drosophila Model of ALS

Nicholas E. Karagas, Richa Gupta, Elham Rastegari, Kai Li Tan, Ho Hang Leung, Hugo J. Bellen, Kartik Venkatachalam and Ching-On Wong
Journal of Neuroscience 19 October 2022, 42 (42) 8019-8037; DOI: https://doi.org/10.1523/JNEUROSCI.2456-21.2022
Nicholas E. Karagas
1Department of Integrative Biology and Pharmacology, McGovern Medical School at the University of Texas Health Sciences Center, Houston, Texas 77030
5Graduate Program in Biochemistry and Cell Biology, MD Anderson Cancer Center and University of Texas Health Sciences Center Graduate School of Biomedical Sciences, Houston, TX, 77030
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Richa Gupta
1Department of Integrative Biology and Pharmacology, McGovern Medical School at the University of Texas Health Sciences Center, Houston, Texas 77030
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Elham Rastegari
1Department of Integrative Biology and Pharmacology, McGovern Medical School at the University of Texas Health Sciences Center, Houston, Texas 77030
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Kai Li Tan
2Departments of Molecular and Human Genetics and Neuroscience, Baylor College of Medicine, Houston, TX 77030
3Graduate Program in Developmental Biology, Baylor College of Medicine, Houston, Texas 77030
4Duncan Neurological Research Institute, Texas Children Hospital, Houston, Texas 77030
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Ho Hang Leung
7Department of Biological Sciences, Rutgers University, Newark, New Jersey 07102
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Hugo J. Bellen
2Departments of Molecular and Human Genetics and Neuroscience, Baylor College of Medicine, Houston, TX 77030
3Graduate Program in Developmental Biology, Baylor College of Medicine, Houston, Texas 77030
4Duncan Neurological Research Institute, Texas Children Hospital, Houston, Texas 77030
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Kartik Venkatachalam
1Department of Integrative Biology and Pharmacology, McGovern Medical School at the University of Texas Health Sciences Center, Houston, Texas 77030
5Graduate Program in Biochemistry and Cell Biology, MD Anderson Cancer Center and University of Texas Health Sciences Center Graduate School of Biomedical Sciences, Houston, TX, 77030
6Graduate Program in Neuroscience, MD Anderson Cancer Center and University of Texas Health Sciences Center Graduate School of Biomedical Sciences, Houston, TX, 77030
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Ching-On Wong
7Department of Biological Sciences, Rutgers University, Newark, New Jersey 07102
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Article Information

DOI 
https://doi.org/10.1523/JNEUROSCI.2456-21.2022
PubMed 
36261266
Published By 
Society for Neuroscience
History 
  • Received December 14, 2021
  • Revision received August 23, 2022
  • Accepted August 27, 2022
  • First published September 8, 2022.
  • Version of record published October 19, 2022.
Copyright & Usage 
Copyright © 2022 the authors SfN exclusive license.

Author Information

  1. Nicholas E. Karagas1,5,
  2. Richa Gupta1,
  3. Elham Rastegari1,
  4. Kai Li Tan2,3,4,
  5. Ho Hang Leung7,
  6. Hugo J. Bellen2,3,4,
  7. Kartik Venkatachalam1,5,6, and
  8. Ching-On Wong7
  1. 1Department of Integrative Biology and Pharmacology, McGovern Medical School at the University of Texas Health Sciences Center, Houston, Texas 77030
  2. 2Departments of Molecular and Human Genetics and Neuroscience, Baylor College of Medicine, Houston, TX 77030
  3. 3Graduate Program in Developmental Biology, Baylor College of Medicine, Houston, Texas 77030
  4. 4Duncan Neurological Research Institute, Texas Children Hospital, Houston, Texas 77030
  5. 5Graduate Program in Biochemistry and Cell Biology, MD Anderson Cancer Center and University of Texas Health Sciences Center Graduate School of Biomedical Sciences, Houston, TX, 77030
  6. 6Graduate Program in Neuroscience, MD Anderson Cancer Center and University of Texas Health Sciences Center Graduate School of Biomedical Sciences, Houston, TX, 77030
  7. 7Department of Biological Sciences, Rutgers University, Newark, New Jersey 07102
  • N. E. Karagas' present address: Department of Neurology, University of Washington, Seattle, Washington 98195.

  • K. L. Tan's present address: Emergent BioSolutions, Gaithersburg, Maryland 20879.

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Author contributions

  1. Author contributions: N.E.K., K.L.T., H.J.B., K.V., and C.-O.W. designed research; N.E.K., R.G., E.R., K.L.T., H.H.L., K.V., and C.-O.W. performed research; N.E.K., K.L.T., K.V., and C.-O.W. analyzed data; N.E.K., K.L.T., H.J.B., K.V., and C.-O.W. edited the paper; K.V. wrote the first draft of the paper; K.V. and C.-O.W. wrote the paper.

  2. R.G. and E.R. contributed equally to this work.

  • N. E. Karagas' present address: Department of Neurology, University of Washington, Seattle, Washington 98195.

  • K. L. Tan's present address: Emergent BioSolutions, Gaithersburg, Maryland 20879.

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Disclosures

    • Received December 14, 2021.
    • Revision received August 23, 2022.
    • Accepted August 27, 2022.
  • This work was supported by the National Institutes of Health Grants R03AG063251 (to C.-O.W.), RF1AG069076 (to K.V.), RF1AG067414 (to K.V.), and R21AG072176 (to K.V.). We thank the Bloomington Drosophila Stock Center for fly stocks. We also thank Yufang Chao for technical help. Confocal and live cell microscopy were performed at the University of Texas Health Sciences Center Center for Advanced Microscopy, Department of Integrative Biology and Pharmacology at McGovern Medical School, and the Advanced Imaging Core, Department of Biological Sciences, Rutgers University, Newark.

  • The authors declare no competing financial interests.

  • Correspondence should be addressed to Kartik Venkatachalam at kartik.venkatachalam{at}uth.tmc.edu or Ching-On Wong at chingon.wong{at}rutgers.edu

Funding

  • HHS | NIH | National Institute on Aging (NIA)

    RF1AG069076; RF1AG067414; R21AG072176; R03AG063251

Other Version

  • previous version (September 08, 2022).
  • You are viewing the most recent version of this article.

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The Journal of Neuroscience: 42 (42)
Journal of Neuroscience
Vol. 42, Issue 42
19 Oct 2022
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Loss of Activity-Induced Mitochondrial ATP Production Underlies the Synaptic Defects in a Drosophila Model of ALS
Nicholas E. Karagas, Richa Gupta, Elham Rastegari, Kai Li Tan, Ho Hang Leung, Hugo J. Bellen, Kartik Venkatachalam, Ching-On Wong
Journal of Neuroscience 19 October 2022, 42 (42) 8019-8037; DOI: 10.1523/JNEUROSCI.2456-21.2022

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Loss of Activity-Induced Mitochondrial ATP Production Underlies the Synaptic Defects in a Drosophila Model of ALS
Nicholas E. Karagas, Richa Gupta, Elham Rastegari, Kai Li Tan, Ho Hang Leung, Hugo J. Bellen, Kartik Venkatachalam, Ching-On Wong
Journal of Neuroscience 19 October 2022, 42 (42) 8019-8037; DOI: 10.1523/JNEUROSCI.2456-21.2022
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Keywords

  • ALS
  • Drosophila neurobiology
  • ER calcium channels
  • mitochondrial ATP production
  • neurodegeneration
  • VAPB

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