The whole-mount image shows the motor axons (NF-positive; red) innervating the neuromuscular junctions (α-bungarotoxin; white) in the dilator nasi muscle of the rat. The positive synaptophysin signals (green) are colocalized with the innervated neuromuscular junctions. This staining demonstrates active synaptic transmission of the innervated neuromuscular junctions. For more information, see the article by Tereshenko V et al. (pages 8297–8307). Cover image: Roland Blumer and Vlad Tereshenko.