Figure 7. Deletion of p53 partially rescues the phenotype in Chd8 deficient mice. A, Coronal sections of E12.5 cortices from control, Chd8 cKO, and Chd8 cKO animals heterozygous for p53 knock-out (Chd8 cKO; p53 cHet), and Chd8; p53 dKO mice immunostained for C-Casp3. Scale bar, 100 µm. DAPI, nuclei counterstain (blue). B, Quantification of C-Casp3+ cells from control, Chd8 cKO, Chd8 cKO; p53 cHet, and Chd8; p53 dKO mice. Data are means ± SEM (n = 6 animals per genotype); ***p < 0.001, n.s., no significance; one-way ANOVA followed by Tukey's multiple comparisons test. C, Coronal sections of E14.5 cortices from control, Chd8 cKO, Chd8 cKO; p53 cHet, and Chd8; p53 dKO mice immunostained for PH3. Scale bar, 200 µm. D) Quantification of PH3+ cells in VZ region from cortices of control, Chd8 cKO, Chd8 cKO; p53 cHet, and Chd8; p53 dKO mice. Data are means ± SEM (n = 6 animals per genotype); *p < 0.05, **p < 0.01, ***p < 0.001; one-way ANOVA followed by Tukey's multiple comparisons test. E, Coronal sections of E14.5 cortices from control, Chd8 cKO, Chd8 cKO; p53 cHet, and Chd8; p53 dKO mice immunostained for TBR2, Scale bar, 200 µm. F, Quantification of TBR2+ cells from cortices of control, Chd8 cKO, Chd8 cKO; p53 cHet, and Chd8; p53 dKO mice. Data are means ± SEM (n = 6 animals per genotype); *p < 0.05, **p < 0.01, ***p < 0.001; one-way ANOVA followed by Tukey's multiple comparisons test.