Figure 3. Transient lighting during the latter half of cVA stimulations modulated the responses of Or67d OSNs. A, WT with its antennal nerve amputated. B, WT whose head except for the antenna being recorded was painted black. C, eya2 mutant. D, hdcJK910 mutant. E, longGMR-GAL4/UAS-TNT. Left panels, The firing rates in each 300 ms bin. Blue lines indicate 1.5 s of the lighting period. Red lines indicate the timing of 3 s odor stimulations. Light blue lines indicate the firing rate when both odor and light stimulations were applied. Gray lines indicate those where odor stimulations were solely applied. Right panels, The firing rates during Periods I, II, and III, as shown in Figure 1B. For statistical analyses, Wilcoxon matched-pairs signed rank tests were performed. Error bars indicate SEM. F–I, Expression patterns of longGMR-GAL4. Photoreceptors in the ocelli (arrowheads in F) and eyelet (arrowheads in G–I) neurons as well as in the retinae are labeled. Magenta in G and white in H represent anti-choline acetyltransferase (ChAT) antibodies, whereas white in F and I and green in G represent the presence of anti-GFP antibodies. The area surrounded by dotted square in G is enlarged in H and I. Dorsal side is on the top, lateral is represented on the left (G–I). OL, Optic lobe. Single frontal confocal sections (H,I) and 3D-reconstructed images (F,G) are shown. Scale bars: H, 10 µm; F, G, 100 µm. Genotype: w; +/UAS-GFP; UAS-20XmCD8::GFP/longGMR-GAL4.