Astrocytes (labeled for GFAP, green) surrounding a cerebrovessel (red blood cell nuclei labeled with DAPI and pseudo-colored red) in mouse barrel cortex (formalin fixed). Image acquired using a Zeiss LSM 880 with a 63x objective. Three-dimensional image rendering was performed with Imaris software. For more information, see the article by Sompol et al. (pages 1797–1813). Cover image: Pradoldej Sompol.