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Research Articles, Cellular/Molecular

Inducible CRISPR Epigenome Systems Mimic Cocaine Induced Bidirectional Regulation of Nab2 and Egr3

Eric Y. Choi, Daniela Franco, Catherine A. Stapf, Madeleine Gordin, Amanda Chow, Kara K. Cover, Ramesh Chandra and Mary Kay Lobo
Journal of Neuroscience 29 March 2023, 43 (13) 2242-2259; DOI: https://doi.org/10.1523/JNEUROSCI.1802-22.2022
Eric Y. Choi
1Department of Anatomy and Neurobiology
2Graduate Program in Life Sciences, Biochemistry and Molecular Biology
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Daniela Franco
1Department of Anatomy and Neurobiology
3Program in Neuroscience, Graduate Program in Life Sciences
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Catherine A. Stapf
1Department of Anatomy and Neurobiology
3Program in Neuroscience, Graduate Program in Life Sciences
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Madeleine Gordin
1Department of Anatomy and Neurobiology
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Amanda Chow
1Department of Anatomy and Neurobiology
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Kara K. Cover
1Department of Anatomy and Neurobiology
3Program in Neuroscience, Graduate Program in Life Sciences
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Ramesh Chandra
1Department of Anatomy and Neurobiology
4Center for Innovative Biomedical Resources, Virus Vector Core, University of Maryland School of Medicine Baltimore, Maryland, 21201
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Mary Kay Lobo
1Department of Anatomy and Neurobiology
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Article Information

DOI 
https://doi.org/10.1523/JNEUROSCI.1802-22.2022
PubMed 
36849419
Published By 
Society for Neuroscience
History 
  • Received September 19, 2022
  • Revision received December 6, 2022
  • Accepted December 22, 2022
  • First published February 27, 2023.
  • Version of record published March 29, 2023.
Copyright & Usage 
Copyright © 2023 the authors SfN exclusive license.

Author Information

  1. Eric Y. Choi1,2,
  2. Daniela Franco1,3,
  3. Catherine A. Stapf1,3,
  4. Madeleine Gordin1,
  5. Amanda Chow1,
  6. Kara K. Cover1,3,
  7. Ramesh Chandra1,4, and
  8. Mary Kay Lobo1
  1. 1Department of Anatomy and Neurobiology
  2. 2Graduate Program in Life Sciences, Biochemistry and Molecular Biology
  3. 3Program in Neuroscience, Graduate Program in Life Sciences
  4. 4Center for Innovative Biomedical Resources, Virus Vector Core, University of Maryland School of Medicine Baltimore, Maryland, 21201
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Author contributions

  1. Author contributions: E.Y.C., R.C., and M.K.L. designed research; E.Y.C., C.A.S., M.G., A.C., K.K.C., and R.C. performed research; E.Y.C., D.F., R.C., and M.K.L. analyzed data; E.Y.C. and D.F. wrote the first draft of the paper; E.Y.C. and M.K.L. edited the paper; E.Y.C. and M.K.L. wrote the paper.

Disclosures

    • Received September 19, 2022.
    • Revision received December 6, 2022.
    • Accepted December 22, 2022.
  • This work was supported by the National Institutes of Health Grants R21 DA046227, R21 DA052101, and R01 DA038613 to M.K.L. and F31 DA052967 to E.Y.C. We thank Alexandros Poulopoulos, Ryan Richardson, Thomas A. Blanpied, and Aaron D. Levy (University of Maryland School of Medicine) for advice and shared resources for Light Plate Apparatus experiments.

  • The authors declare no competing financial interests.

  • Correspondence should be addressed to Ramesh Chandra at rchandra{at}som.umaryland.edu or Mary Kay Lobo at mklobo{at}som.umaryland.edu

Funding

  • HHS | NIH | National Institute on Drug Abuse (NIDA)

    F31DA052967; R21DA046227; R21DA052101; R01DA038613

Other Version

  • previous version (February 27, 2023).
  • You are viewing the most recent version of this article.

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The Journal of Neuroscience: 43 (13)
Journal of Neuroscience
Vol. 43, Issue 13
29 Mar 2023
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Inducible CRISPR Epigenome Systems Mimic Cocaine Induced Bidirectional Regulation of Nab2 and Egr3
Eric Y. Choi, Daniela Franco, Catherine A. Stapf, Madeleine Gordin, Amanda Chow, Kara K. Cover, Ramesh Chandra, Mary Kay Lobo
Journal of Neuroscience 29 March 2023, 43 (13) 2242-2259; DOI: 10.1523/JNEUROSCI.1802-22.2022

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Inducible CRISPR Epigenome Systems Mimic Cocaine Induced Bidirectional Regulation of Nab2 and Egr3
Eric Y. Choi, Daniela Franco, Catherine A. Stapf, Madeleine Gordin, Amanda Chow, Kara K. Cover, Ramesh Chandra, Mary Kay Lobo
Journal of Neuroscience 29 March 2023, 43 (13) 2242-2259; DOI: 10.1523/JNEUROSCI.1802-22.2022
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Keywords

  • addiction
  • cocaine
  • CRISPR
  • drug
  • Egr3
  • Nab2

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