Figure 1B. Self-administration | Food | | | |
Repeated-measures ANOVA | Session (1–6), within subjects | F(5, 50) = 0.9 | 0.46 | 0.09 |
| Fentanyl | | | |
| Session (1–12), within subjects | F(11 110) = 1.1 | 0.37 | 0.10 |
Figure 1C. Food vs. fentanyl choice | Preference Score | | | |
Repeated-measures ANOVA | Session (1–10), within subjects | F(9,90) = 6.6 | <0.001* | 0.40 |
Figure 1D. Relapse test | Lever (active, inactive), within subjects | F(1,4) = 18.3 | 0.01* | 0.82 |
Total responding | | | | |
Repeated-measures ANOVA | | | | |
Figure 1D. Relapse test | Session Time (30, 60, 90), within subjects | F(2,8) = 13.0 | 0.003* | 0.76 |
Time course | Lever (active, inactive), within subjects | F(1,4) = 18.3 | 0.01* | 0.82 |
Repeated-measures ANOVA | Session Time × Lever interaction | F(2,8) = 11.9 | 0.004* | 0.75 |
Figure 1E. Fos and CTb counting | AI | | | |
Univariate ANOVA | Fos | | | |
| Test Condition (Test, No Test), between subjects | F(1,9) = 6.8 | 0.03* | 0.43 |
| CTb | | | |
| Test Condition (Test, No Test), between subjects | F(1,9) = 1.3 | 0.29 | 0.13 |
| Fos plus CTb | | | |
| Test Condition (Test, No Test), between subjects | F(1,9) = 7.4 | 0.02* | 0.45 |
| PL | | | |
| Fos | | | |
| Test Condition (Test, No Test), between subjects | F(1,9) = 6.6 | 0.03* | 0.42 |
| CTb | | | |
| Test Condition (Test, No Test), between subjects | F(1,9) = 2.3 | 0.16 | 0.20 |
| Fos plus CTb | | | |
| Test Condition (Test, No Test), between subjects | F(1,9) = 11.0 | 0.009* | 0.55 |
| IL | | | |
| Fos | | | |
| Test Condition (Test, No Test), between subjects | F(1,9) = 1.6 | 0.24 | 0.15 |
| CTb | | | |
| Test Condition (Test, No Test), between subjects | F(1,9) = 0.6 | 0.47 | 0.06 |
| Fos plus CTb | | | |
| Test Condition (Test, No Test), between subjects | F(1,9) = 6.0 | 0.04* | 0.40 |
| BLA | | | |
| Fos | | | |
| Test Condition (Test, No Test), between subjects | F(1,9) = 0.7 | 0.43 | 0.07 |
| CTb | | | |
| Test Condition (Test, No Test), between subjects | F(1,9) = 0.0 | 0.86 | 0.00 |
| Fos plus CTb | | | |
| Test Condition (Test, No Test), between subjects | F(1,9) = 0.1 | 0.82 | 0.01 |
Figure 2B. Self-administration | Food | | | |
Repeated-Measures ANOVA | Session (1–6), within subjects | F(5155) = 1.0 | 0.42 | 0.03 |
| Fentanyl | | | |
| Session (1–12), within subjects | F(11 341) = 12.5 | <0.001* | 0.29 |
Figure 2C. Food vs. fentanyl choice Repeated-Measures ANOVA | Preference score | | | |
| Session (1–12), within subjects | F(11 341) = 7.5 | <0.001* | 0.20 |
Figure 2D. Relapse test | Drug Condition (vehicle, M + B ipsi, M + B contra), between subjects | F(2,29) = 5.2 | 0.01* | 0.26 |
Total responding | Lever (active, inactive) within subjects | F(1,29) = 204.8 | <0.001* | 0.88 |
Mixed ANOVA | Drug Condition × Lever interaction | F(2,29) = 4.4 | 0.02* | 0.23 |
Figure 2D. Relapse test | Drug Condition (vehicle, M + B ipsi, M + B contra), between subjects | F(2,29) = 5.2 | 0.01* | 0.26 |
Time course | Session Time (30, 60, 90) within subjects | F(2,58) = 85.6 | <0.001* | 0.75 |
Mixed ANOVA | Lever (active, inactive), within subjects | F(1,29) = 204.8 | <0.001* | 0.88 |
| Drug Condition × Session Time interaction | F(4,58) = 1.3 | 0.29 | 0.08 |
| Drug Condition × Lever interaction | F(2,29) = 4.4 | 0.02* | 0.23 |
| Session Time × Lever interaction | F(2,58) = 83.9 | <0.001* | 0.74 |
| Drug Condition × Session Time × Lever interaction | F(4,58) = 1.9 | 0.12 | 0.12 |
Figure 2E. Reacquisition | Drug Condition (vehicle, M + B ipsi, M + B contra), between subjects | F(2,29) = 1.3 | 0.28 | 0.08 |
Mixed ANOVA | Session Hour (1–6) within subjects | F(5145) = 10.7 | <0.001* | 0.27 |
| Drug Condition × Session hour interaction | F(10 145) = 0.6 | 0.79 | 0.04 |
Figure 3B. Self-administration | Food | | | |
Repeated-measures ANOVA | Session (1–6), within subjects | F(5200) = 3.8 | 0.003* | 0.09 |
| Fentanyl | | | |
| Session (1–12), within subjects | F(11 440) = 35.2 | <0.001* | 0.47 |
Figure 3C. Food vs fentanyl choice Repeated-measures ANOVA | Preference Score | | | |
| Session (1–12), within subjects | F(11 440) = 8.6 | <0.001* | 0.18 |
Figure 3D. Relapse test | Drug Condition (vehicle, M + B ipsi, M + B contra), between subjects | F(2,35) = 0.2 | 0.85 | 0.01 |
Total responding | Lever (active, inactive) within subjects | F(1,35) = 182.4 | <0.001* | 0.84 |
Repeated-measures ANOVA | Drug Condition × Lever interaction | F(2,35) = 0.0 | 0.97 | 0.00 |
Figure 3D. Relapse test | Drug Condition (vehicle, M + B ipsi, M + B contra), between subjects | F(2,35) = 0.2 | 0.85 | 0.01 |
Time course | Session Time (30, 60, 90) within subjects | F(2,70) = 103.6 | <0.001* | 0.75 |
Repeated-measures ANOVA | Lever (active, inactive), within subjects | F(1,35) = 182.4 | <0.001* | 0.84 |
| Drug Condition × Session Time interaction | F(4,70) = 1.4 | 0.23 | 0.08 |
| Drug Condition × Lever interaction | F(2,35) = 0.0 | 0.97 | 0.00 |
| Session Time × Lever interaction | F(2,70) = 96.3 | <0.001* | 0.73 |
| Drug Condition × Session Time × Lever interaction | F(4,70) = 1.3 | 0.26 | 0.07 |
Figure 3E. Reacquisition | Drug Condition (vehicle, M + B ipsi, M + B contra), between subjects | F(2,38) = 3.5 | 0.04* | 0.16 |
Mixed ANOVA | Session Hour (1–6) within subjects | F(5190) = 5.9 | <0.001* | 0.13 |
| Drug Condition × Session hour interaction | F(10 190) = 1.3 | 0.23 | 0.07 |
Figure 4B. Self-administration | Food | | | |
Repeated-measures ANOVA | Session (1–6), within subjects | F(5,65) = 3.8 | 0.004* | 0.23 |
| Fentanyl | | | |
| Session (1–12), within subjects | F(11 143) = 2.7 | 0.004* | 0.17 |
Figure 4C. Food vs. fentanyl choice Repeated-measures ANOVA | Preference score | | | |
| Session (1–12), within subjects | F(11 143) = 5.0 | <0.001* | 0.28 |
Figure 4D. Relapse test | Lever (active, inactive) within subjects | F(1,13) = 47.9 | <0.001* | 0.79 |
Total responding | | | | |
Repeated-measures ANOVA | | | | |