Immunohistochemistry for astrocyte-specific GCaMP6f (green), glial fibrillary acidic protein (GFAP; red), and DAPI (blue). This technique was used to analyze the overlap (yellow) between the GCaMP6f and astrocytes in the basolateral amygdala (BLA) to ensure selective expression for fiber photometry recordings during fear learning. For more information, see the article by Suthard, Senne et al. (pages 4997–5013). Cover image: Steve Ramirez.