Skip to main content

Main menu

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Collections
    • Podcast
  • ALERTS
  • FOR AUTHORS
    • Information for Authors
    • Fees
    • Journal Clubs
    • eLetters
    • Submit
    • Special Collections
  • EDITORIAL BOARD
    • Editorial Board
    • ECR Advisory Board
    • Journal Staff
  • ABOUT
    • Overview
    • Advertise
    • For the Media
    • Rights and Permissions
    • Privacy Policy
    • Feedback
    • Accessibility
  • SUBSCRIBE

User menu

  • Log out
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Neuroscience
  • Log out
  • Log in
  • My Cart
Journal of Neuroscience

Advanced Search

Submit a Manuscript
  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Collections
    • Podcast
  • ALERTS
  • FOR AUTHORS
    • Information for Authors
    • Fees
    • Journal Clubs
    • eLetters
    • Submit
    • Special Collections
  • EDITORIAL BOARD
    • Editorial Board
    • ECR Advisory Board
    • Journal Staff
  • ABOUT
    • Overview
    • Advertise
    • For the Media
    • Rights and Permissions
    • Privacy Policy
    • Feedback
    • Accessibility
  • SUBSCRIBE
PreviousNext
Featured ArticleResearch Articles, Systems/Circuits

Molecular, Circuit, and Stress Response Characterization of Ventral Pallidum Npas1-Neurons

Gessynger Morais-Silva, Rianne R. Campbell, Hyungwoo Nam, Mahashweta Basu, Marco Pagliusi, Megan E. Fox, C. Savio Chan, Sergio D. Iñiguez, Seth Ament, Nathan Cramer, Marcelo Tadeu Marin and Mary Kay Lobo
Journal of Neuroscience 18 January 2023, 43 (3) 405-418; https://doi.org/10.1523/JNEUROSCI.0971-22.2022
Gessynger Morais-Silva
1Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore, Maryland 21201
2Sao Paulo State University (UNESP), School of Pharmaceutical Sciences, Laboratory of Pharmacology, Araraquara, Sao Paulo 14800903, Brazil
3Joint Graduate Program in Physiological Sciences, Federal University of São Carlos/Sao Paulo State University, CEP 13565-905, São Carlos/Araraquara, Brazil
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Gessynger Morais-Silva
Rianne R. Campbell
1Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore, Maryland 21201
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hyungwoo Nam
1Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore, Maryland 21201
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mahashweta Basu
4Department of Psychiatry, University of Maryland School of Medicine, Baltimore, Maryland 21201
5Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, Maryland 21201
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Marco Pagliusi
1Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore, Maryland 21201
6Department of Structural and Functional Biology, State University of Campinas, SP-13083-872, Campinas, Brazil
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Megan E. Fox
1Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore, Maryland 21201
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
C. Savio Chan
7Department of Neuroscience, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for C. Savio Chan
Sergio D. Iñiguez
8Department of Psychology, University of Texas at El Paso, El Paso, Texas 79902
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Sergio D. Iñiguez
Seth Ament
4Department of Psychiatry, University of Maryland School of Medicine, Baltimore, Maryland 21201
5Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, Maryland 21201
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nathan Cramer
1Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore, Maryland 21201
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Nathan Cramer
Marcelo Tadeu Marin
2Sao Paulo State University (UNESP), School of Pharmaceutical Sciences, Laboratory of Pharmacology, Araraquara, Sao Paulo 14800903, Brazil
3Joint Graduate Program in Physiological Sciences, Federal University of São Carlos/Sao Paulo State University, CEP 13565-905, São Carlos/Araraquara, Brazil
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mary Kay Lobo
1Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore, Maryland 21201
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Mary Kay Lobo
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Article Figures & Data

Figures

  • Extended Data
  • Figure 1.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 1.

    Representative images of the injection site in the VP. A, The first column (from the left) shows the expression of the eYFP (in green), while the second column shows the expression of the tdTomato in Npas1 neurons (in red). The third column shows the merged images. B, Representative of the injection site in the VP at low magnification (2.5×). Scale bar, 1 mm. ac, Anterior commissure; lv, lateral ventricle; 3v, third ventricle.

  • Figure 2.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 2.

    Representative images of DIO-eYFP and tdTomato expression in multiple brain regions of the Npas1-cre mouse. The first and fourth columns (from the left) show the expression of the eYFP (in green) within the brain regions, while the second and fifth columns show the expression of the tdTomato (in red). The third and sixth columns show the merged images. Scale bar, 100 µm. ac, Anterior commissure; LS, lateral septum; MS, medial septum; Hb, habenula; MHb, medial habenula; PVT, paraventricular nucleus of the thalamus; LH, lateral hypothalamus; opt, optical nerve; Thal, thalamus; LV, lateral ventricle; 3V, third ventricle; PAG, periaqueductal gray; Aq, cerebral aqueduct.

  • Figure 3.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 3.

    Cell type-specific profiling of mRNA from VP Npas1+ neurons reveal potential functions and regulators these neurons. A, Validation of Npas1 enrichment in ribosome-associated mRNA from VP Npas1+ neurons used in genetic profiling. B, Volcano plot of DEGs within Npas+ VP cells versus non-cell type-specific VP cells. Supporting data listing the significantly enriched genes from VP Npas1+ neurons compared with nonspecific VP neurons are available in Extended Data Figure 3-1. C, Top hits from Biological Process and Molecular Function GO (Gene Ontology) term analysis and an example from one of the top hits, regulation of neurogenesis, with the degree of fold change of genes within the GO term. Supporting data listing top hits from Biological Process and Molecular Function GO term analysis in VP Npas1+ neurons are available in Extended Data Figure 3-2. D, Two top hits from upstream regulator prediction analysis using the top two GO terms identified, neurogenesis and development of nervous system. Predicted DEG targets of each regulator are identified with arrows along with their fold change analyzed in VP Npas1+ neurons.

  • Figure 4.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 4.

    Validation of DREADDs activation on Npas1+ neurons. A, Representative current-clamp recordings from VP Npas1+ neurons in vitro expressing the hM4Di (left) or hM3Dq (right) receptor. Following CNO wash-on, hM4Di cells (n = 4) showed a decrease in spiking, and hM3Dq cells (n = 3) showed an increase in spiking in response to depolarizing current steps. B, A subset of hM3Dq cells (n = 8) that did not have an increase in spiking displayed enhanced depolarization but also a reduction in membrane resistance. *p < 0.05.

  • Figure 5.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 5.

    Chemogenetic activation of hM3Dq receptors in VP Npas1+ neurons increases susceptibility to the SSDS in male mice. A, Representative timeline of the experiments involving the manipulation of VP Npas1+ neurons during the SSDS. B–D, Effects of the chemogenetic activation of hM3Dq receptors in VP Npas1+ neurons during the SSDS in the SI test and FST. E–G, Effects of chemogenetic activation of hM4Di receptors in VP Npas1+ neurons during the SSDS in the SI and FST. Data are presented as the mean plus the individual values obtained for each animal (n = 5–9 animals/group). *p < 0.05, **p < 0.01, ***p < 0.001.

  • Figure 6.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 6.

    Chemogenetic activation of hM4Di receptors in VP Npas1+ neurons decreases susceptibility to the CSDS in male mice. A, Representative timeline of the experiments involving the activation of hM4Di receptors in VP Npas1+ neurons during the CSDS. B–D, Effects of the chemogenetic activation of hM4Di receptors in VP Npas1+ neurons during the CSDS in the SI and FST. Data are presented as the mean plus the individual values obtained for each animal (n = 6–8 animals/group). *p < 0.05, **p < 0.01, ***p < 0.001.

  • Figure 7.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 7.

    Chemogenetic activation of hM3Dq receptors in VP Npas1+ neurons is anxiogenic in male mice. A, Representative timeline of the experiments involving the activation of hM3Dq receptors in VP Npas1+ neurons during behavioral tests that evaluate anxiety-like and depressive-like behaviors. B–E, effects of chemogenetic activation of hM3Dq receptors in VP Npas1+ neurons during the EPM test. F, G, effects of the chemogenetic activation of hM3Dq receptors in VP Npas1+ neurons during the OFT. H, I, Effects of the chemogenetic activation of hM3Dq receptors in VP Npas1+ neurons during the SI. J, Effects of chemogenetic activation of hM3Dq receptors in VP Npas1+ neurons during the ST. K, L, Effects of chemogenetic activation of hM3Dq receptors in VP Npas1+ neurons on the sucrose preference and intake on the SPT. Data are presented as the mean plus the individual values obtained for each animal (n = 8–9 animals/group). *p < 0.05.

  • Figure 8.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 8.

    Chemogenetic activation of hM4Di receptors in VP Npas1+ neurons decreases the susceptibility to CWDS in female mice. A, Representative timeline of the experiments involving the activation of hM4Di receptors in VP Npas1+ neurons during the CWDS. B, Effects of the chemogenetic activation of hM4Di receptors in VP Npas1+ neurons during the CWDS in the three-chamber social preference test. Data are presented as the mean plus the individual values obtained for each animal (n = 6–10 animals/group). *p < 0.05.

Extended Data

  • Figures
  • Figure 3-1

    List of significantly enriched genes from VP Npas1+ neurons compared with nonspecific VP neurons. Download Figure 3-1, XLSX file.

  • Figure 3-2

    Top hits from Biological Process and Molecular Function GO (Gene Ontology) term analysis in VP Npas1+ neurons. Download Figure 3-2, XLSX file.

Back to top

In this issue

The Journal of Neuroscience: 43 (3)
Journal of Neuroscience
Vol. 43, Issue 3
18 Jan 2023
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Index by author
  • Masthead (PDF)
Email

Thank you for sharing this Journal of Neuroscience article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Molecular, Circuit, and Stress Response Characterization of Ventral Pallidum Npas1-Neurons
(Your Name) has forwarded a page to you from Journal of Neuroscience
(Your Name) thought you would be interested in this article in Journal of Neuroscience.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Print
View Full Page PDF
Citation Tools
Molecular, Circuit, and Stress Response Characterization of Ventral Pallidum Npas1-Neurons
Gessynger Morais-Silva, Rianne R. Campbell, Hyungwoo Nam, Mahashweta Basu, Marco Pagliusi, Megan E. Fox, C. Savio Chan, Sergio D. Iñiguez, Seth Ament, Nathan Cramer, Marcelo Tadeu Marin, Mary Kay Lobo
Journal of Neuroscience 18 January 2023, 43 (3) 405-418; DOI: 10.1523/JNEUROSCI.0971-22.2022

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Respond to this article
Request Permissions
Share
Molecular, Circuit, and Stress Response Characterization of Ventral Pallidum Npas1-Neurons
Gessynger Morais-Silva, Rianne R. Campbell, Hyungwoo Nam, Mahashweta Basu, Marco Pagliusi, Megan E. Fox, C. Savio Chan, Sergio D. Iñiguez, Seth Ament, Nathan Cramer, Marcelo Tadeu Marin, Mary Kay Lobo
Journal of Neuroscience 18 January 2023, 43 (3) 405-418; DOI: 10.1523/JNEUROSCI.0971-22.2022
Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Introduction
    • Materials and Methods
    • Results
    • Discussion
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Keywords

  • depression
  • DREAADs
  • Npas1
  • social defeat stress
  • ventral pallidum

Responses to this article

Respond to this article

Jump to comment:

No eLetters have been published for this article.

Related Articles

Cited By...

More in this TOC Section

Research Articles

  • Functional Roles of Gastrin-Releasing Peptide-Producing Neurons in the Suprachiasmatic Nucleus: Insights into Photic Entrainment and Circadian Regulation
  • Brain Topological Changes in Subjective Cognitive Decline and Associations with Amyloid Stages
  • The Functional Anatomy of Nociception: Effective Connectivity in Chronic Pain and Placebo Response
Show more Research Articles

Systems/Circuits

  • Hippocampal Sharp-Wave Ripples Decrease during Physical Actions Including Consummatory Behavior in Immobile Rodents
  • Developmental Olfactory Dysfunction and Abnormal Odor Memory in Immune-Challenged Disc1+/− Mice
  • Functional Roles of Gastrin-Releasing Peptide-Producing Neurons in the Suprachiasmatic Nucleus: Insights into Photic Entrainment and Circadian Regulation
Show more Systems/Circuits
  • Home
  • Alerts
  • Follow SFN on BlueSky
  • Visit Society for Neuroscience on Facebook
  • Follow Society for Neuroscience on Twitter
  • Follow Society for Neuroscience on LinkedIn
  • Visit Society for Neuroscience on Youtube
  • Follow our RSS feeds

Content

  • Early Release
  • Current Issue
  • Issue Archive
  • Collections

Information

  • For Authors
  • For Advertisers
  • For the Media
  • For Subscribers

About

  • About the Journal
  • Editorial Board
  • Privacy Notice
  • Contact
  • Accessibility
(JNeurosci logo)
(SfN logo)

Copyright © 2025 by the Society for Neuroscience.
JNeurosci Online ISSN: 1529-2401

The ideas and opinions expressed in JNeurosci do not necessarily reflect those of SfN or the JNeurosci Editorial Board. Publication of an advertisement or other product mention in JNeurosci should not be construed as an endorsement of the manufacturer’s claims. SfN does not assume any responsibility for any injury and/or damage to persons or property arising from or related to any use of any material contained in JNeurosci.