Skip to main content

Main menu

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Collections
    • Podcast
  • ALERTS
  • FOR AUTHORS
    • Information for Authors
    • Fees
    • Journal Clubs
    • eLetters
    • Submit
  • EDITORIAL BOARD
  • ABOUT
    • Overview
    • Advertise
    • For the Media
    • Rights and Permissions
    • Privacy Policy
    • Feedback
  • SUBSCRIBE

User menu

  • Log in
  • My Cart

Search

  • Advanced search
Journal of Neuroscience
  • Log in
  • My Cart
Journal of Neuroscience

Advanced Search

Submit a Manuscript
  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Collections
    • Podcast
  • ALERTS
  • FOR AUTHORS
    • Information for Authors
    • Fees
    • Journal Clubs
    • eLetters
    • Submit
  • EDITORIAL BOARD
  • ABOUT
    • Overview
    • Advertise
    • For the Media
    • Rights and Permissions
    • Privacy Policy
    • Feedback
  • SUBSCRIBE
PreviousNext
Featured ArticleResearch Articles, Cellular/Molecular

Dual Regulation of Spine-Specific and Synapse-to-Nucleus Signaling by PKCδ during Plasticity

Lesley A. Colgan, Paula Parra-Bueno, Heather L. Holman, Xun Tu, Anant Jain, Mariah F. Calubag, Jaime A. Misler, Chancellor Gary, Goksu Oz, Irena Suponitsky-Kroyter, Elwy Okaz and Ryohei Yasuda
Journal of Neuroscience 26 July 2023, 43 (30) 5432-5447; DOI: https://doi.org/10.1523/JNEUROSCI.0208-22.2023
Lesley A. Colgan
Neuronal Signal Transduction, Max Planck Florida Institute for Neuroscience, Jupiter, Florida 33458
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Paula Parra-Bueno
Neuronal Signal Transduction, Max Planck Florida Institute for Neuroscience, Jupiter, Florida 33458
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Heather L. Holman
Neuronal Signal Transduction, Max Planck Florida Institute for Neuroscience, Jupiter, Florida 33458
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xun Tu
Neuronal Signal Transduction, Max Planck Florida Institute for Neuroscience, Jupiter, Florida 33458
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anant Jain
Neuronal Signal Transduction, Max Planck Florida Institute for Neuroscience, Jupiter, Florida 33458
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mariah F. Calubag
Neuronal Signal Transduction, Max Planck Florida Institute for Neuroscience, Jupiter, Florida 33458
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jaime A. Misler
Neuronal Signal Transduction, Max Planck Florida Institute for Neuroscience, Jupiter, Florida 33458
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chancellor Gary
Neuronal Signal Transduction, Max Planck Florida Institute for Neuroscience, Jupiter, Florida 33458
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Goksu Oz
Neuronal Signal Transduction, Max Planck Florida Institute for Neuroscience, Jupiter, Florida 33458
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Goksu Oz
Irena Suponitsky-Kroyter
Neuronal Signal Transduction, Max Planck Florida Institute for Neuroscience, Jupiter, Florida 33458
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Elwy Okaz
Neuronal Signal Transduction, Max Planck Florida Institute for Neuroscience, Jupiter, Florida 33458
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ryohei Yasuda
Neuronal Signal Transduction, Max Planck Florida Institute for Neuroscience, Jupiter, Florida 33458
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Ryohei Yasuda
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

The activity-dependent plasticity of synapses is believed to be the cellular basis of learning. These synaptic changes are mediated through the coordination of local biochemical reactions in synapses and changes in gene transcription in the nucleus to modulate neuronal circuits and behavior. The protein kinase C (PKC) family of isozymes has long been established as critical for synaptic plasticity. However, because of a lack of suitable isozyme-specific tools, the role of the novel subfamily of PKC isozymes is largely unknown. Here, through the development of fluorescence lifetime imaging-fluorescence resonance energy transfer activity sensors, we investigate novel PKC isozymes in synaptic plasticity in CA1 pyramidal neurons of mice of either sex. We find that PKCδ is activated downstream of TrkB and DAG production, and that the spatiotemporal nature of its activation depends on the plasticity stimulation. In response to single-spine plasticity, PKCδ is activated primarily in the stimulated spine and is required for local expression of plasticity. However, in response to multispine stimulation, a long-lasting and spreading activation of PKCδ scales with the number of spines stimulated and, by regulating cAMP response-element binding protein activity, couples spine plasticity to transcription in the nucleus. Thus, PKCδ plays a dual functional role in facilitating synaptic plasticity.

SIGNIFICANCE STATEMENT Synaptic plasticity, or the ability to change the strength of the connections between neurons, underlies learning and memory and is critical for brain health. The protein kinase C (PKC) family is central to this process. However, understanding how these kinases work to mediate plasticity has been limited by a lack of tools to visualize and perturb their activity. Here, we introduce and use new tools to reveal a dual role for PKCδ in facilitating local synaptic plasticity and stabilizing this plasticity through spine-to-nucleus signaling to regulate transcription. This work provides new tools to overcome limitations in studying isozyme-specific PKC function and provides insight into molecular mechanisms of synaptic plasticity.

  • CREB
  • FRET
  • PKC
  • plasticity
  • sensor
  • synapse

SfN exclusive license.

View Full Text

Member Log In

Log in using your username and password

Enter your Journal of Neuroscience username.
Enter the password that accompanies your username.
Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
Back to top

In this issue

The Journal of Neuroscience: 43 (30)
Journal of Neuroscience
Vol. 43, Issue 30
26 Jul 2023
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Index by author
  • Masthead (PDF)
Email

Thank you for sharing this Journal of Neuroscience article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Dual Regulation of Spine-Specific and Synapse-to-Nucleus Signaling by PKCδ during Plasticity
(Your Name) has forwarded a page to you from Journal of Neuroscience
(Your Name) thought you would be interested in this article in Journal of Neuroscience.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Print
View Full Page PDF
Citation Tools
Dual Regulation of Spine-Specific and Synapse-to-Nucleus Signaling by PKCδ during Plasticity
Lesley A. Colgan, Paula Parra-Bueno, Heather L. Holman, Xun Tu, Anant Jain, Mariah F. Calubag, Jaime A. Misler, Chancellor Gary, Goksu Oz, Irena Suponitsky-Kroyter, Elwy Okaz, Ryohei Yasuda
Journal of Neuroscience 26 July 2023, 43 (30) 5432-5447; DOI: 10.1523/JNEUROSCI.0208-22.2023

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Respond to this article
Request Permissions
Share
Dual Regulation of Spine-Specific and Synapse-to-Nucleus Signaling by PKCδ during Plasticity
Lesley A. Colgan, Paula Parra-Bueno, Heather L. Holman, Xun Tu, Anant Jain, Mariah F. Calubag, Jaime A. Misler, Chancellor Gary, Goksu Oz, Irena Suponitsky-Kroyter, Elwy Okaz, Ryohei Yasuda
Journal of Neuroscience 26 July 2023, 43 (30) 5432-5447; DOI: 10.1523/JNEUROSCI.0208-22.2023
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Introduction
    • Materials and Methods
    • Results
    • Discussion
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Keywords

  • CREB
  • FRET
  • PKC
  • plasticity
  • sensor
  • synapse

Responses to this article

Respond to this article

Jump to comment:

No eLetters have been published for this article.

Related Articles

Cited By...

More in this TOC Section

Research Articles

  • Optogenetics reveals roles for supporting cells in force transmission to and from outer hair cells in the mouse cochlea
  • Pre-saccadic neural enhancements in marmoset area MT
  • Interareal synaptic inputs underlying whisking-related activity in the primary somatosensory barrel cortex
Show more Research Articles

Cellular/Molecular

  • Optogenetics reveals roles for supporting cells in force transmission to and from outer hair cells in the mouse cochlea
  • Chronic desipramine reverses deficits in cell activity, norepinephrine innervation and anxiety-depression phenotypes in fluoxetine-resistant cF1ko mice
  • P2Y6 Receptor-Dependent Microglial Phagocytosis of Synapses during Development Regulates Synapse Density and Memory
Show more Cellular/Molecular
  • Home
  • Alerts
  • Visit Society for Neuroscience on Facebook
  • Follow Society for Neuroscience on Twitter
  • Follow Society for Neuroscience on LinkedIn
  • Visit Society for Neuroscience on Youtube
  • Follow our RSS feeds

Content

  • Early Release
  • Current Issue
  • Issue Archive
  • Collections

Information

  • For Authors
  • For Advertisers
  • For the Media
  • For Subscribers

About

  • About the Journal
  • Editorial Board
  • Privacy Policy
  • Contact
(JNeurosci logo)
(SfN logo)

Copyright © 2023 by the Society for Neuroscience.
JNeurosci Online ISSN: 1529-2401

The ideas and opinions expressed in JNeurosci do not necessarily reflect those of SfN or the JNeurosci Editorial Board. Publication of an advertisement or other product mention in JNeurosci should not be construed as an endorsement of the manufacturer’s claims. SfN does not assume any responsibility for any injury and/or damage to persons or property arising from or related to any use of any material contained in JNeurosci.