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Featured ArticleResearch Articles, Cellular/Molecular

Indirect Effects of Halorhodopsin Activation: Potassium Redistribution, Nonspecific Inhibition, and Spreading Depolarization

R. Ryley Parrish, Connie MacKenzie-Gray Scott, Tom Jackson-Taylor, Alex Grundmann, Faye McLeod, Neela K. Codadu, Alexandru Călin, Hannah Alfonsa, Rob C. Wykes, Juha Voipio and Andrew J. Trevelyan
Journal of Neuroscience 1 February 2023, 43 (5) 685-692; https://doi.org/10.1523/JNEUROSCI.1141-22.2022
R. Ryley Parrish
1Newcastle University Biosciences Institute, Medical School, Newcastle upon Tyne, NE2 4HH, United Kingdom
2Department of Cell Biology and Physiology, Brigham Young University, Provo 84602, Utah
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Connie MacKenzie-Gray Scott
1Newcastle University Biosciences Institute, Medical School, Newcastle upon Tyne, NE2 4HH, United Kingdom
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Tom Jackson-Taylor
1Newcastle University Biosciences Institute, Medical School, Newcastle upon Tyne, NE2 4HH, United Kingdom
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Alex Grundmann
1Newcastle University Biosciences Institute, Medical School, Newcastle upon Tyne, NE2 4HH, United Kingdom
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Faye McLeod
1Newcastle University Biosciences Institute, Medical School, Newcastle upon Tyne, NE2 4HH, United Kingdom
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Neela K. Codadu
4Queen Square Institute of Neurology, University College London, WC1N 3BG, United Kingdom
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Alexandru Călin
1Newcastle University Biosciences Institute, Medical School, Newcastle upon Tyne, NE2 4HH, United Kingdom
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Hannah Alfonsa
1Newcastle University Biosciences Institute, Medical School, Newcastle upon Tyne, NE2 4HH, United Kingdom
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Rob C. Wykes
3Nanomedicine Lab, University of Manchester, Manchester, M13 9PL, United Kingdom
4Queen Square Institute of Neurology, University College London, WC1N 3BG, United Kingdom
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Juha Voipio
5Faculty of Biological and Environmental Sciences, Molecular and Integrative Biosciences, University of Helsinki, Helsinki, 00014, Finland
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Andrew J. Trevelyan
1Newcastle University Biosciences Institute, Medical School, Newcastle upon Tyne, NE2 4HH, United Kingdom
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Article Information

DOI 
https://doi.org/10.1523/JNEUROSCI.1141-22.2022
PubMed 
36639898
Published By 
Society for Neuroscience
History 
  • Received June 10, 2022
  • Revision received November 28, 2022
  • Accepted December 2, 2022
  • First published December 9, 2022.
  • Version of record published February 1, 2023.
Copyright & Usage 
Copyright © 2023 the authors SfN exclusive license.

Author Information

  1. R. Ryley Parrish1,2,
  2. Connie MacKenzie-Gray Scott1,
  3. Tom Jackson-Taylor1,
  4. Alex Grundmann1,
  5. Faye McLeod1,
  6. Neela K. Codadu4,
  7. Alexandru Călin1,
  8. Hannah Alfonsa1,
  9. Rob C. Wykes3,4,
  10. Juha Voipio5, and
  11. Andrew J. Trevelyan1
  1. 1Newcastle University Biosciences Institute, Medical School, Newcastle upon Tyne, NE2 4HH, United Kingdom
  2. 2Department of Cell Biology and Physiology, Brigham Young University, Provo 84602, Utah
  3. 3Nanomedicine Lab, University of Manchester, Manchester, M13 9PL, United Kingdom
  4. 4Queen Square Institute of Neurology, University College London, WC1N 3BG, United Kingdom
  5. 5Faculty of Biological and Environmental Sciences, Molecular and Integrative Biosciences, University of Helsinki, Helsinki, 00014, Finland
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Author contributions

  1. Author contributions: R.P., J.V., and A.T. designed research; R.P., C.M., T.J., A.G., F.M., N.C., A.C., H.A., R.W., J.V., and A.T. performed research; R.P., C.M., N.C., A.C., R.W., and A.T. analyzed data; R.P., R.W., and J.V. edited the paper; A.T. wrote the paper.

Disclosures

    • Received June 10, 2022.
    • Revision received November 28, 2022.
    • Accepted December 2, 2022.
  • The work was supported by Biotechnology and Biological Sciences Research Council Grant BB/P019854/1, Medical Research Council Grant MR/R005427/1, and European Union's Horizon 2020 Research and Innovation Program Grant Agreement 881603 (GrapheneCore3). A.C. was supported by National Institute for Health and Care Research, Academic Clinical Fellowship in Neurology. We thank the support staff in the animal facilities at both Newcastle University and University College London; Dr. Anton Guimera-Brunet and Dr. Eduard Masvidal-Codina (Institut de Mircroelectronica de Barcelona, CNM-CSIC) for providing the graphene micro-transistor arrays used in this study; Dr. Martin Smith (University College London) for technical assistance in preparing the mice for the graphene micro-transistor array in vivo experiments; and Joe Raimondo for commenting on drafts of this manuscript and discussion.

  • The authors declare no competing financial interests.

  • Correspondence should be addressed to Andrew J. Trevelyan at andrew.trevelyan{at}ncl.ac.uk

Funding

  • UKRI | MRC | Medical Research Foundation

    MR/R005427/1
  • UKRI | Biotechnology and Biological Sciences Research Council (BBSRC)

    BB/P019854/1
  • European Union Horizon 2020

    Grant Agreement No. 881603

Other Version

  • previous version (December 09, 2022).
  • You are viewing the most recent version of this article.

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Dec 2022608025
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Jul 2025137222
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Total 2025120923304
Total40982622990
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The Journal of Neuroscience: 43 (5)
Journal of Neuroscience
Vol. 43, Issue 5
1 Feb 2023
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Indirect Effects of Halorhodopsin Activation: Potassium Redistribution, Nonspecific Inhibition, and Spreading Depolarization
R. Ryley Parrish, Connie MacKenzie-Gray Scott, Tom Jackson-Taylor, Alex Grundmann, Faye McLeod, Neela K. Codadu, Alexandru Călin, Hannah Alfonsa, Rob C. Wykes, Juha Voipio, Andrew J. Trevelyan
Journal of Neuroscience 1 February 2023, 43 (5) 685-692; DOI: 10.1523/JNEUROSCI.1141-22.2022

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Indirect Effects of Halorhodopsin Activation: Potassium Redistribution, Nonspecific Inhibition, and Spreading Depolarization
R. Ryley Parrish, Connie MacKenzie-Gray Scott, Tom Jackson-Taylor, Alex Grundmann, Faye McLeod, Neela K. Codadu, Alexandru Călin, Hannah Alfonsa, Rob C. Wykes, Juha Voipio, Andrew J. Trevelyan
Journal of Neuroscience 1 February 2023, 43 (5) 685-692; DOI: 10.1523/JNEUROSCI.1141-22.2022
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Keywords

  • chloride
  • halorhodopsin
  • potassium
  • pyramidal cells
  • spreading depression

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