Abstract
The amygdalar anterior basolateral nucleus (BLa) plays a vital role in emotional behaviors. This region receives dense cholinergic projections from basal forebrain which are critical in regulating neuronal activity in BLa. Cholinergic signaling in BLa has also been shown to modulate afferent glutamatergic inputs to this region. However, these studies, which have used cholinergic agonists or prolonged optogenetic stimulation of cholinergic fibers, may not reflect the effect of physiological acetylcholine release in the BLa. To better understand these effects of acetylcholine, we have used electrophysiology and optogenetics in male and female mouse brain slices to examine cholinergic regulation of afferent BLa input from cortex and midline thalamic nuclei. Phasic ACh release evoked by single pulse stimulation of cholinergic terminals had a biphasic effect on transmission at cortical input, producing rapid nicotinic receptor-mediated facilitation followed by slower mAChR-mediated depression. In contrast, at this same input, sustained ACh elevation through application of the cholinesterase inhibitor physostigmine suppressed glutamatergic transmission through mAChRs only. This suppression was not observed at midline thalamic nuclei inputs to BLa. In agreement with this pathway specificity, the mAChR agonist, muscarine more potently suppressed transmission at inputs from prelimbic cortex than thalamus. Muscarinic inhibition at prelimbic cortex input required presynaptic M4 mAChRs, while at thalamic input it depended on M3 mAChR-mediated stimulation of retrograde endocannabinoid signaling. Muscarinic inhibition at both pathways was frequency-dependent, allowing only high-frequency activity to pass. These findings demonstrate complex cholinergic regulation of afferent input to BLa that is pathway-specific and frequency-dependent.
SIGNIFICANCE STATEMENT Cholinergic modulation of the basolateral amygdala regulates formation of emotional memories, but the underlying mechanisms are not well understood. Here, we show, using mouse brain slices, that ACh differentially regulates afferent transmission to the BLa from cortex and midline thalamic nuclei. Fast, phasic ACh release from a single optical stimulation biphasically regulates glutamatergic transmission at cortical inputs through nicotinic and muscarinic receptors, suggesting that cholinergic neuromodulation can serve precise, computational roles in the BLa. In contrast, sustained ACh elevation regulates cortical input through muscarinic receptors only. This muscarinic regulation is pathway-specific with cortical input inhibited more strongly than midline thalamic nuclei input. Specific targeting of these cholinergic receptors may thus provide a therapeutic strategy to bias amygdalar processing and regulate emotional memory.
Introduction
The basolateral amygdala is a brain region central to emotional processing and is necessary for associating cues with both positive and negative valence outcomes (LeDoux et al., 1990; Baxter and Murray, 2002; Janak and Tye, 2015). Compared with other brain regions, the basolateral amygdala, especially the anterior subdivision of the basolateral nucleus (BLa), receives the densest cholinergic projections from basal forebrain (BF) (Woolf, 1991; Muller et al., 2011; Zaborszky et al., 2012), suggesting that acetylcholine (ACh) plays a central role in regulating neurons in this region. Indeed, cholinergic mechanisms in the BLa are important modulators of emotional memory (Power et al., 2003; McGaugh, 2004; Jiang et al., 2016; Wilson and Fadel, 2017). Cholinergic mechanisms in the BLa are also thought to regulate reward devaluation learning (Salinas et al., 1997), performance in tests of anxiety and depression-like behaviors (Mineur et al., 2016, 2018), and conditioned cue reinstatement of cocaine seeking (See et al., 2003; See, 2005), suggesting roles for ACh in the BLa in anxiety and fear disorders and drug addiction. These findings underscore the impact of cholinergic activity in the BLa in the pathophysiology of several neuropsychiatric diseases and highlight the need to better understand cholinergic modulation of the BLa.
ACh is thought to modulate neuronal circuits through both a slow mode of volume transmission as well as a more temporally precise phasic mode and thereby regulate neural activity over a range of temporal and spatial scales (Disney and Higley, 2020; Sarter and Lustig, 2020). These two modes of cholinergic transmission likely engage different types of ACh receptors with different kinetics, affinities, desensitization characteristics, and cellular locations. Thus, the nature of the cholinergic response may depend on the dynamics of ACh release. In the BLa, cholinergic signaling shapes neural activity through multiple mechanisms, including the regulation of presynaptic release probability (Jiang and Role, 2008; Jiang et al., 2016). Cholinergic projections from the BF converge with excitatory terminals providing an anatomic basis for cholinergic regulation of glutamatergic transmission in this area (Muller et al., 2011, 2013). As in other brain regions, ACh in the BLa is thought to act on presynaptic nicotinic receptors to enhance glutamate release (Jiang and Role, 2008; Jiang et al., 2016) and on muscarinic receptors to suppress release (Sugita et al., 1991; Yajeya et al., 2000). However, prior studies examining cholinergic modulation have used exogenous agonists or sustained optogenetic stimulation of cholinergic afferents, which results in broad spatial and temporal activation of cholinergic receptors. Evidence that cholinergic regulation can occur in a rapid and precise timescale sufficient to modulate individual synaptic events is lacking. This is significant as cholinergic neurons in BF can exhibit fast and precise responses to behaviorally relevant cues (Hangya et al., 2015; Crouse et al., 2020). Furthermore, little is known about the types of cholinergic receptors engaged by different modes of release or the relative role of ACh in modulating different afferent inputs to this region.
In the present study, we have investigated cholinergic regulation of afferent input to the BLa in mouse brain slices. The BLa receives major excitatory projections from prelimbic cortex (PL) and midline thalamic nuclei (MTN), which are thought to play distinct roles in amygdalar-dependent behaviors (Corcoran and Quirk, 2007; Arruda-Carvalho and Clem, 2014; Salay et al., 2018; Amir et al., 2019; Ahmed et al., 2021). We find that endogenously released ACh from single pulse optical stimulation can rapidly and precisely regulate glutamatergic transmission at cortical inputs, suggesting that cholinergic neuromodulation can serve precise, computational roles in the BLa at this timescale. This modulation differs from that during a sustained elevation of ACh, indicating involvement of different ACh receptors. During sustained ACh, cholinergic regulation is pathway-specific, producing stronger regulation of cortical than thalamic input. It is also frequency-dependent and acts as a high pass filter for incoming signals. Through these mechanisms, ACh dynamically shapes afferent input to BLa to bias amygdalar processing of salient cues.
Materials and Methods
Animals
All experiments were performed in adult ChAT-Cre mice (B6;129S6-Chattm2(cre)Lowl/J; JAX stock #006410) of either sex. These mice express Cre-recombinase under the control of the choline acetyltransferase gene. Alternately, in some experiments, the adult F1 progeny of ChAT-Cre mice crossed with Ai32 mice (B6;129S-Gt(ROSA)26SORtm21(CAG-COP4*H134R/EYFP)Hze/J, JAX stock #012569) were used. Mice were group-housed in a climate-controlled facility with a 12/12 light/dark cycle and provided with ad libitum access to food and water. All animal care and use procedures were approved by the University of South Carolina's Institutional Animal Care and Use Committee and performed in compliance with the guidelines approved by the National Institutes of Health's Guide for the care and use of laboratory animals (Department of Health and Human Services).
AAV delivery
Mice 1.5-3 months old were anesthetized under deep isoflurane anesthesia and placed in a stereotaxic surgery device (Stoelting). For ex vivo slice electrophysiology experiments using released ACh, 0.2 μl of rAAV5-EF1a-DIO-hChR2(H134R)-EYFP (UNC Viral Vector Core) was delivered bilaterally into the BF, including the ventral pallidum/substantia innominata (from bregma: AP 1.2 mm; ML ±1.3 mm; DV −5.3 mm), the main source of cholinergic inputs to the BLa. For ex vivo slice electrophysiology experiments examining PL input to the BLa, 0.15 μl of rAAV5-CAMKII-hChR2(H134R)-EYFP-WPRE (UNC Viral Vector Core) was delivered bilaterally to the PL (from bregma: AP 1.9 mm; ML ±0.3 mm; DV −2.0 mm). For experiments examining MTN input to the BLa, single injections of 0.2 μl of rAAV5-CAMKII-hChR2(H134R)-EYFP-WPRE (UNC Viral Vector Core) were delivered to the MTN (from bregma: AP −0.3 mm; ML 0.0 mm; DV −3.9 mm). For experiments examining ventral subicular (vSUB) input to the BLa, single injections of 0.2 μl of rAAV5-CAMKII-hChR2(H134R)-EYFP-WPRE (UNC Viral Vector Core) were delivered to the vSUB (from bregma: AP −2.5 mm; ML ±3.2 mm; DV −5.3 mm). Mice were used for experiments at least 3 weeks after surgery.
Immunofluorescence
To validate expression of channelrhodopsin in BF cholinergic neurons, ChAT-Cre mice injected in the BF with rAAV5-EF1a-DIO-hChR2(H134R)-EYFP or ChAT-Cre/Ai32 mice were transcardially perfused with ice-cold PBS containing 0.5% nitrite followed with 4% PFA. Brains were postfixed overnight in 4% PFA at 4°C; 50 μm coronal brain sections were cut using a vibratome (VT1200S, Leica). Slices were blocked in TBS containing 0.5% Triton X-100 and 10% normal donkey serum and incubated for 30 min at room temperature. Sections were then incubated for 48 h at room temperature in goat anti-ChAT primary antibody (1:1000, AB144P, Millipore). Following rinse, sections were again incubated at room temperature for 3 h in TBS containing an AlexaFluor-546-conjugated donkey anti-goat IgG secondary antibody (1:400, A-11056, Fisher Scientific), 0.5% Triton X-100, and 2% normal donkey serum. Sections were rinsed and mounted on slides with ProLong diamond antifade mountant DAPI (Fisher Scientific, P36971) and imaged on a Leica SP8 Multiphoton confocal microscope (Leica Microsystems). ChR2-EYFP was assessed by the endogenous EYFP fluorescent signal. The number of neurons positive for both EYFP and ChAT, or EYFP alone, were counted in each image using ImageJ (National Institutes of Health).
Slice preparation
Mice were deeply anesthetized with isoflurane and the brain quickly extracted and submerged in ice-cold (4°C) “cutting” ACSF containing the following (in mm): 110 choline chloride, 2.5 KCL, 25 NaHCO2, 1.0 NaH2PO4, 20 glucose, 5 MgCl2, 0.5 CaCl2, and continuously bubbled with 95% O2/5% CO2. Choline chloride-based cutting solution with choline as a sodium-ion replacement has been previously used for slice electrophysiological recordings, as it preserves health of the slices (Hoffman and Johnston, 1998; Suzuki and Momiyama, 2021; Perumal and Sah, 2022). Brains were cut into 300-μm-thick (for whole-cell experiments) or 500-μm-thick (for field recordings) coronal sections using a vibratome (VT1000S, Leica). Slices were transferred to an incubation chamber filled with warmed ACSF containing the following (in mm): 125 NaCl, 2.7 KCl, 25 NaHCO2, 1.25 NaH2PO4, 10 glucose, 5 MgCl2, 0.5 CaCl2, and bubbled with 95% O2/5% CO2 at 34°C–36°C. After a minimum of 20 min, the incubation temperature was allowed to equilibrate to room temperature for at least 40 min before slices were used for recording.
Slice electrophysiology recordings
For recording, slices were placed in a submersion chamber and continuously perfused with oxygenated (95% O2/5% CO2), recording ACSF a rate of 4–6 ml/min (for field potentials) or 1–2 ml/min (for whole cell) at 30°C–32°C. Recording ACSF contained the following (in mm): 125 NaCl, 2.7 KCl, 25 NaHCO2, 1.25 NaH2PO4, 10 glucose, 1 MgCl2, 2 CaCl2 (pH 7.4, 305 mOsm). Field potentials were recorded from the BLa with an Axoprobe 1A amplifier (Molecular Devices) using borosilicate glass electrodes, which had a resistance of 1–3 mΩ when filled with recording ACSF. For whole-cell recording, pyramidal neurons in the BLa were visualized using infrared-differential interference contrast optics through a 40× objective (Olympus BX51WI). Borosilicate glass electrodes of 4–6 mΩ resistance were used for recordings and filled with a potassium gluconate internal solution consisting of the following (in mm): 135 K-gluconate, 5 KCl, 10 HEPES, 2 MgCl2, 2 MgATP, 0.3 NaGTP, and 0.5 EGTA. Voltage-clamp recordings were made at a holding potential of −70 mV with a Multiclamp 700B (Molecular Devices) amplifier. Experiments were discarded if significant changes occurred in input or series resistance which were monitored throughout. All responses were filtered at 1 kHz, digitized using a Digidata 1440A A-D board (Molecular Devices) and analyzed using pClamp10 software (Molecular Devices).
To evoke glutamatergic field EPSPs (fEPSPs), PL or MTN fibers expressing hChR2(H134R)-EYFP were stimulated with single or dual (50 ms apart) pulses (1–3 ms duration) of 490 nm blue LED light (M490F3, ThorLabs) delivered through a fiber optic cable directly over the recording site in the BLa every 30 s. For whole-cell recording, the pulse of 490 nm blue light (pE-4000, CoolLED) was delivered through the 40× objective. Alternately, in some experiments, fEPSPs or whole-cell EPSCs in BLa were electrically evoked using a 0.1 ms current pulse delivered through a monopolar platinum-iridium stimulating electrode (FHC) placed in the external capsule (EC). A two-opsin strategy to independently activate PL and MTN inputs was not used due, in part, to concerns about cross-talk between the opsins under the conditions of our field potential experiments (Venkatachalam and Cohen, 2014; Christoffel et al., 2021). Non-NMDA glutamatergic responses were pharmacologically isolated by blocking GABAA receptors (10–100 μm picrotoxin or 10 μm bicuculline), GABAB receptors (2 μm CGP55845), and NMDA receptors (50 μm D-APV or 10 μm MK801). CNQX (25 μm) was added at the conclusion of some experiments to confirm that the response was mediated by AMPA/kainate receptors.
To study the effects of released ACh, cholinergic fibers expressing hChR2(H134R)-EYFP were optogenetically stimulated with 2–3 ms pulses of 490 nm blue LED light delivered directly over the recording site in the BLa every 90 s. A single light pulse was delivered either immediately (2 ms) before or 250 ms before electrical stimulation. Alternately, a theta burst of light [4 bursts of light (four pulses at 50 Hz) every 200 ms] was delivered 250 ms before electrical stimulation (such that the last light pulse in the burst was 250 ms before electrical stimulation). In whole-cell experiments to determine the effect of light on the EPSC amplitude, any direct postsynaptic currents produced by optically released ACh alone were recorded and subtracted from evoked EPSC traces where light was applied.
Drugs
Drugs used in this study are listed in Table 1. Baclofen, muscarine chloride, N-ethylmaleimide, mecamylamine, physostigmine, and telenzepine were purchased from Millipore Sigma. Bicuculline, D-AP5, CNQX, CGP55845 hydrochloride, MK 801 maleate, picrotoxin, and AM251 were purchased from HelloBio. WIN 55212-2 and oxotremorine-M were purchased from Tocris Bioscience. 4DAMP, AF-DX 116, VU0255025, and atropine were purchased from Abcam. AM630 was purchased from Cayman Chemical, and VU0467154 was purchased from StressMarq Biosciences. All reagents were added from freshly prepared stock solution to the ACSF. Drugs were applied using bath perfusion and drug concentration in the bath during wash-in was allowed to equilibrate before measurements were taken.
Data analysis and statistics
Electrophysiological data analysis was performed using pClamp 10 (Molecular Devices) and OriginPro 2018b (Microcal) software. For released ACh experiments, consecutive sweeps in “Light ON” or “Light OFF” conditions (2–6 sweeps) were averaged and the peak amplitude of the averaged EPSC or fEPSP was measured. For experiments involving optogenetically stimulated PL and MTN input and bath application of muscarine, the peak amplitude of fEPSPs was measured as the average peak amplitude of the steady-state evoked fEPSP response in each pharmacological condition. All peak amplitudes were normalized to the baseline condition (“control”) and are expressed as the mean ± SEM. Concentration–response curves represent a least-squares fit of each dataset to a sigmoidal (logistic) curve (GraphPad Prism, GraphPad Software). The IC50 and Hill slope were calculated from this curve. Means, SEs and 95% CIs were determined by the fitting algorithm. In some experiments, multiple slices per animal were used, so for all experiments, n = slice number and N = animal number. Statistical significance was determined using a Student's t test (paired or unpaired), a one-way ANOVA, or a repeated-measures ANOVA with a post hoc Tukey test (α < 0.05 was taken as significant).
Results
Immunofluorescent verification of ChR2 expression in BLa-projecting cholinergic neurons in the BF
In order to optogenetically evoke ACh release, two strategies were used to selectively express channelrhodopsin in BF cholinergic axons in BLa. First, ChR2-EYFP was expressed in BF cholinergic neurons of ChAT-Cre mice through Cre-dependent rAAV-mediated transfection (Unal et al., 2015; Aitta-aho et al., 2018). Four weeks after AAV injection, we verified selective ChR2-EYFP expression in neurons labeled with ChAT antibody (ChAT+) in the BF (Fig. 1A,B). Cell counts of ChAT+ neurons, ChR2-EYFP+ neurons (ChR2+), or neurons expressing both ChAT+ and ChR2-EYFP+ at the injection site revealed that most ChAT+ neurons expressed ChR2-EYFP (70.2 ± 4.26%, N = 5, Fig. 1B). Furthermore, immunoreactivity for ChAT in the majority of ChR2-EYFP+ cells (89.8 ± 2.4%, N = 5) confirmed that expression of ChR2 was restricted to cholinergic neurons in this region (Fig. 1B, bottom). Axons from labeled ChAT+ neurons in BF densely innervated the BLa (Fig. 1Av), as previously described (Aitta-aho et al., 2018), further supporting the selective labeling of cholinergic projections to BLa.
Channelrhodopsin was also expressed in ChAT+ neurons using a double transgenic strategy in which ChAT-Cre mice were crossed with an Ai32 reporter mouse line expressing Cre-dependent ChR2-EYFP (Hedrick et al., 2016; Baker et al., 2018). In the F1 generation of these mice (ChAT-Cre/Ai32 mice), the majority of BF ChAT+ neurons (80 ± 3%, 877 cells, N = 3) were immunopositive for ChR2-EYFP. Furthermore, immunoreactivity for ChAT in most ChR2-EYFP-immunopositive cells (99.1 ± 0.8%, 694 cells, N = 3) confirmed that expression of ChR2 was restricted to cholinergic neurons (Fig. 1C,D). Notably, the BLa of these mice did not contain any cell bodies positive for ChR2, ensuring selective activation of BF derived cholinergic terminals with optogenetic stimulation during BLa slice recordings.
Synaptically released ACh biphasically regulates cortico-amygdalar transmission in the BLa through both nicotinic and muscarinic receptors
In vivo recordings indicate that a behaviorally relevant cue can recruit BF cholinergic neurons to synchronously fire a single, precisely timed spike or brief burst of action potentials (Hangya et al., 2015). To determine the effect of this cholinergic neuron activity on afferent input to the BLa, cholinergic terminals were stimulated with a single blue light pulse (490 nm) and the effect on synaptic transmission at cortical inputs to BLa in ChAT-Cre/Ai32 mice examined. EPSCs were evoked in BLa pyramidal cells by electrical stimulation of cortical afferents in the EC (Fig. 2A) (Jiang et al., 2016). Optogenetic stimulation of cholinergic terminals had a biphasic effect on the amplitude of this EPSC in the majority of cells (Fig. 2). Stimulation of cholinergic terminals immediately (2 ms) before stimulation of cortical afferents (Early interval) evoked a facilitation of the EPSC. This early facilitation was sensitive to the frequency at which cholinergic terminals were stimulated. In these experiments, cholinergic terminals were stimulated every 90 s, as stimulation at shorter intervals resulted in a rundown or loss of the facilitation. The extent of the facilitation varied between cells (range: 92%-137%, mean: 107.5 ± 2%, n = 23, N = 10) with 17 of 23 cells (73.9%) exhibiting a facilitation (Fig. 2C). Increasing the interval between the light pulse and cortical afferent stimulation caused this facilitation to rapidly diminish and become a depression at intervals >20 ms. When the cortical afferents were stimulated 250 ms after the light pulse, the EPSC was suppressed (range: 46%-96%; mean: 79.4 ± 3%; n = 17, N = 10; Fig. 2C,D) with 17 of 17 cells (100%) showing inhibition. All cells that exhibited early facilitation also exhibited late inhibition. However, EPSCs in five cells exhibited late inhibition with no early facilitation. Late inhibition was similar in amplitude whether cholinergic terminals were stimulated with a single light pulse or a theta burst [4 bursts of light (four pulses at 50 Hz) delivered every 200 ms] of light pulses (Fig. 2D).
Pharmacological analysis revealed that the early facilitation by ACh was completely blocked by the nicotinic antagonist, mecamylamine (10 μm; Fig. 3A), indicating that it was nAChR-mediated. Mecamylamine had no effect on the EPSC amplitude at the 250 ms interval (Fig. 3C), demonstrating the absence of any delayed effect of nAChRs on the EPSC, as has been reported in cortex (Urban-Ciecko et al., 2018). The rundown of this nicotinic response at stimulus intervals <90 s is consistent with nAChR desensitization or depletion of transmitter in the presynaptic cholinergic terminal, as has been reported in hypothalamus (Hatton and Yang, 2002). However, other factors, such as presynaptic inhibition of ACh release, may also contribute (Zhang et al., 2002). The site of action of nAChRs was investigated by examining the effect of cholinergic stimulation on paired pulse facilitation. Nicotinic facilitation significantly reduced the paired pulse ratio at the early interval (Fig. 3B), indicating a presynaptic site of action in agreement with prior studies (Jiang and Role, 2008; Cheng and Yakel, 2014; Tang et al., 2015). In contrast, late cholinergic suppression of the EPSC was blocked by bath application of the muscarinic antagonist, atropine (5 μm; Fig. 3C), demonstrating that it was mAChR-mediated. This mAChR-mediated depression of the EPSC significantly increased the paired pulse ratio at the later interval (Fig. 3D), suggesting that the mAChRs were also presynaptic.
A similar cholinergic-induced late inhibition of cortical-evoked transmission was also evident in field potential recordings in the BLa. As shown in Figure 3E, EC stimulation evoked fEPSPs in BLa that reflected EPSCs in pyramidal neurons during whole-cell recording. Optogenetic stimulation of cholinergic terminals with theta burst stimulation [4 bursts of light (four pulses at 50 Hz) delivered every 200 ms] significantly inhibited the fEPSP evoked by EC stimulation 250 ms later. Theta burst stimulation of cholinergic terminals was subsequently delivered every 90 s to study cholinergic inhibition of the cortical fEPSP. Theta burst stimulation was chosen for these studies to reflect BF activity during active waking and paradoxical sleep (Lee et al., 2005). Cholinergic inhibition of the fEPSP was unaffected by mecamylamine (10 μm) but was completely reversed by application of atropine (5 μm; Fig. 3F), indicating that it was muscarinic receptor-mediated. Together, these findings suggest that single pulse stimulation of ACh terminals evokes a biphasic modulation of cortical input by ACh, whereby ACh acts via a precisely timed action on presynaptic nAChRs to rapidly facilitate cortical neurotransmission to the BLa and on presynaptic mAChRs to cause a delayed suppression. Similarly, an mAChR-mediated delayed suppression of fEPSPs is also seen following theta pattern stimulation of ACh release.
ACh differentially regulates cortical and thalamic input to the BLa
A behaviorally salient cue can recruit BF cholinergic neurons to fire, producing a phasic release of ACh into BLa (Aitta-aho et al., 2018; Crouse et al., 2020). In contrast, during prolonged emotional arousal, extracellular ACh levels in the amygdala exhibit a sustained increase (Kellis et al., 2020). To investigate the impact of sustained ACh on synaptic transmission, we increased endogenous, extracellular ACh by applying physostigmine to inhibit acetylcholinesterase, the enzyme that catalyzes the breakdown of ACh. We compared the effect of increasing concentrations (0.3–10 μm) of physostigmine on the amplitude of the EC-evoked fEPSP. Blocking AChE led to a concentration-dependent suppression of the EC-evoked fEPSP (Fig. 4A,B). Antagonism of muscarinic receptors (5 μm atropine) reversed this suppression, indicating that the inhibition was muscarinic receptor-mediated. The ability of AChE inhibition alone to suppress the EC-evoked fEPSP in the absence of stimulation of cholinergic inputs demonstrates the presence of endogenously released ACh in the brain slice and suggests that the impact of released ACh on synaptic transmission in this pathway is limited by this enzyme, in line with previous studies (Aitta-aho et al., 2018).
Inputs from both cortex and MTN exert significant influence over BLa activity to regulate amygdalar responses to emotionally arousing stimuli (Corcoran and Quirk, 2007; Arruda-Carvalho and Clem, 2014; Salay et al., 2018; Amir et al., 2019; Ahmed et al., 2021). Cholinergic mechanisms have the potential to play a significant role in shaping afferent input through these pathways. However, the relative role of ACh in regulating transmission in these pathways has not been examined. To compare cholinergic regulation of thalamic and cortical inputs, we injected an rAAV containing ChR2-EYFP under the control of the CaMKII promoter into the MTN of mice. After at least 3 weeks, brain slices were prepared and glutamatergic terminals in BLa from MTN and cortex were stimulated in the same slice and evoked field responses recorded at the same site. MTN fEPSPs were evoked by optogenetic stimulation of MTN terminals in BLa with single pulses of blue light, while cortical fEPSPs were evoked by electrical stimulation of cortical afferents in the EC (Fig. 3E). The effect of a sustained increase in ACh was assessed in each pathway following application of physostigmine (10 μm). As previously observed (Fig. 4A), elevated ACh strongly suppressed the cortical fEPSP (Fig. 4B). This inhibition was blocked by atropine (5 μm), indicating that it was mediated by muscarinic receptors. Subsequent application of mecamylamine (10 μm) had no additional effect, suggesting that nicotinic receptors were not involved. In contrast, at the same recording site, elevation of ACh with physostigmine had no significant effect on baseline responses to MTN pathway stimulation. However, application of atropine significantly increased the MTN fEPSP, and this increase was blocked by mecamylamine. These findings suggest that, at baseline, elevated ACh engaged both muscarine and nicotinic receptors at MTN inputs to produce opposing and offsetting effects on the fEPSP. Application of atropine blocked the muscarinic inhibition, revealing the unopposed nicotinic facilitation which was subsequently blocked by mecamylamine. These differences in response to physostigmine in the two pathways could be caused, in part, by differences in the electrical versus optical method of stimulation. To evaluate this possibility, we examined the effect of physostigmine on fEPSPs evoked by optogenetic stimulation of PL inputs to BLa. These experiments were conducted in a separate group of mice that had been injected in PL cortex 4 weeks earlier with an rAAV containing ChR2-EYFP under the control of the CaMKII promoter. Results from these optogenetic experiments (Fig. 4B, right) were similar to results obtained using electrical stimulation of cortical input, indicating that differences in the effect of physostigmine in the two pathways is not caused by differences in the stimulation method. Overall, these findings reveal distinct effects of muscarinic and nicotinic receptors in cortical and MTN pathways. During sustained elevation of ACh, cortical input was strongly inhibited by muscarinic receptors, but little affected by nicotinic receptors. In contrast, thalamic input was more strongly facilitated by nicotinic receptors with markedly less muscarinic inhibition than at cortical inputs.
Differential regulation of cortical and thalamic input to the BLa by muscarinic receptors
To better examine pathway-specific differences in the effect of ACh, we injected an rAAV containing ChR2-EYFP under the control of the CaMKII promoter into either the PL or the MTN of mice. After 3-4 weeks, brain slices were prepared and the effect of muscarine, a selective mAChR agonist, on fEPSPs evoked by a single blue light pulse to either PL or MTN terminals in the BLa was examined. Muscarine (10 μm) inhibited fEPSPs in both the PL and MTN pathways with no sex-dependent difference at either input (% Control; PL males 18.2 + 2.2% (n = 26, N = 26); female 20.8 + 4.2% (n = 6, N = 6), p = 0.6, Student's t test; MTN males 51.8 + 6.4% (n = 14, N = 14), female 39.2 + 4.5% (n = 13, N = 13), p = 0.13, Student's t test) so data were collapsed across males and females for all experiments. Increasing concentrations of muscarine (0.03-30 μm) produced a monotonic decrease in the amplitude of the fEPSP at both inputs (Fig. 5A). The effect of muscarine on PL-evoked fEPSPs could be fit to a standard logistic equation yielding an IC50 of 0.56 μm (95% CI 0.38-0.80 μm) and Hill coefficient of 0.58 (95% CI 0.48-0.68; n = 5-35 slices). Similar analysis of the MTN-evoked fEPSP indicated that the effect of muscarine in this pathway was shifted ∼10 fold to the right (IC50 = 6.04 (95% CI 3.66-9.23 μm), Hill coefficient = 0.56 (95% CI 0.40-0.79; n = 4-27). The CIs of the IC50 concentrations at these two inputs did not overlap indicating that PL input was significantly more sensitive to inhibition by muscarine than was MTN input.
Input to BLa from ventral subiculum (vSub) also plays an important role in regulating amygdalar responses to emotionally arousing stimuli. Given the differing effects of muscarine at PL and MTN inputs, we also assessed muscarine inhibition of input from vSub. fEPSPs were evoked by optogenetic stimulation of vSub terminals in BLa 4 weeks after injection into vSub of AAV containing ChR2-EYFP. Muscarine (10 μm) strongly inhibited these fEPSPs, similar to its effect on PL-evoked fEPSPs, but significantly greater than its inhibition of MTN inputs (Fig. 5B). As observed following EC stimulation (Fig. 3D), the effect of muscarine on both PL and MTN inputs was presynaptic, since muscarine significantly enhanced the paired pulse ratio in both pathways (Fig. 5C,D). Together, these results indicate the presynaptic nature of muscarinic inhibition and that PL and vSUB input to BLa are significantly more sensitive to this inhibition than MTN input.
ACh acts via M3 and M4 receptors to suppress transmission
To identify the mAChR subtype(s) involved in the muscarine-mediated inhibition of the PL- and MTN-evoked fEPSP, we used a protocol in which 10 min of baseline recording was followed by perfusion with muscarine (10 μm) to inhibit the fEPSP before addition of selective muscarinic receptor antagonists. Each drug was perfused until a steady-state effect was observed before moving to the next drug. PL or MTN inputs were optogenetically stimulated, and AMPAR fEPSPs were isolated using 10 μm picrotoxin, 2 μm CGP55845, and 50 μm APV or 10 μm MK-801 to block GABA and NMDA receptors. M1 receptors are the most abundant mAChR in the BLa and have been reported to be present at presynaptic glutamatergic terminals (Muller et al., 2013). However, at both the PL and MTN inputs, the selective M1 receptor antagonist, telenzepine (100 nm; pKi = 8.46), at a concentration shown to inhibit the effects of muscarine (10 μm) in other systems (Christofi et al., 1991; Liu et al., 1998), had no significant effect on the fEPSP in the presence of muscarine. To further rule out a role for M1 receptors, we tested the effect of VU0255035 (5 μm; pKi = 7.8), a selective M1 receptor antagonist with >75-fold selectivity over M2-M5 receptors (Sheffler et al., 2009). VU0255035 (IC50 = 132.6 ± 28.5 nm) was used at a concentration of 5 μm (Bell et al., 2013; Grafe et al., 2021) as this concentration has been reported to block carbachol (CCh, 10 μm)-induced potentiation of NMDA currents (Sheffler et al., 2009) and CCh-induced neuronal depolarization (Xiang et al., 2012; Kurowski et al., 2015), demonstrating its effectiveness. Nevertheless, like telenzepine, VU0255035 also did not produce significant reversal of muscarinic inhibition in either pathway. Consequently, the effect of these two antagonists was combined (Fig. 6A,B) and indicated little functional involvement of M1 receptors in the muscarinic inhibition. The involvement of M2 receptors was tested using the highly selective M2 receptor antagonist AF-DX 116 (pKi = 6.7). AF-DX 116 (0.1-1 μm) blocks ACh (10 μm)-induced inhibition of transmitter release in hippocampus (Raiteri et al., 1990; Goswamee and McQuiston, 2019) and CCh induced suppression of EPSPs in cortex (Gigout et al., 2012). However, in BLa, AF-DX 116 (1 μm) had no effect on muscarinic inhibition of fEPSPs in either pathway (Fig. 6A,B), indicating that M2 receptors were not involved.
In contrast, the M3 antagonist 4-DAMP (1 μm, pKi = 9.3) completely reversed muscarinic inhibition at both pathways to the BLa (Fig. 6A,B). While 4-DAMP is considered an M3 antagonist, it shows limited selectivity over M1, M4, and M5 receptors (Dorje et al., 1991; Moriya et al., 1999; Watson et al., 1999). However, the inability of selective M1 or M2 receptor antagonists to block muscarinic inhibition and the lack of evidence supporting M5 receptors in the BLa (Lebois et al., 2018) suggest that 4-DAMP must block muscarinic inhibition by acting on either M3 or M4 receptors.
To investigate any contribution of M4 receptors to inhibition at PL and/or MTN input, we used the highly selective M4-positive allosteric modulator (M4 PAM) VU0467154 (VU154). In these experiments, a low dose of muscarine (0.3 μm) was initially applied followed by VU154 (3 μm). At the PL pathway, inhibition by this low dose of muscarine was significantly enhanced after application of the M4 PAM (Fig. 6C), indicating that presynaptic M4 receptors are present on PL terminals and inhibit glutamatergic transmission in this pathway. VU0154 (3 μm) also facilitated inhibition produced by another muscarinic agonist, oxotremorine. The M4 PAM increased oxotremorine (0.3 μm)-induced inhibition from 18.7 ± 2.8% in baseline to 60.4 ± 8.6% in the presence of the M4 PAM (n = 5; N = 5; p = 0.013, paired t test). In contrast, the M4 PAM had no effect on either muscarine-induced (Fig. 6D) or oxotremorine (5 μm)-induced inhibition (18.9 ± 2.5% inhibition in oxotremorine, 18.2 ± 1.1% inhibition in oxotremorine + VU154; n = 3; N = 3; p = 0.76, paired t test) at the MTN input. Together, these experiments suggest that M4 receptors contribute to muscarinic inhibition at PL input to BLa, while inhibition at MTN inputs is exclusively mediated by M3 receptors.
Muscarine inhibits synaptic transmission in the PL pathway through Gi/o protein-coupled M4 mAChRs
Because M3 receptors couple to Gq proteins and M4 receptors to Gi/o proteins, treating slices with an agent that inhibits Gi/o proteins should distinguish between inhibitory effects mediated by M3 and M4 receptors. Therefore, to further confirm a role for M4 receptors in producing inhibition in the PL pathway, we assessed the effect of Gi/o protein inactivation by bath application of the sulfhydryl alkylating agent n-ethylmaleimide (NEM) on the effects of muscarine (Shapiro et al., 1994; Morishita et al., 1997). Baclofen, a GABAB receptor agonist that inhibits glutamate release through a Gi/o-coupled mechanism in the BLa (Yamada et al., 1999), served as a positive control. As expected, baclofen (10 μm) significantly inhibited the fEPSP evoked by optogenetic stimulation of the PL input, and this inhibition was reversed by the selective GABAB antagonist, CGP55845 (2 μm, Fig. 7A). In separate experiments, we then used a protocol in which 10 min of baseline recording was followed by perfusion with muscarine (10 μm) to inhibit the PL-evoked fEPSP and establish the baseline level of muscarinic inhibition. Muscarine was then washed out and NEM (50 μm) (Shapiro et al., 1994) was bath-applied to slices for a minimum of 15 min. Muscarine (10 μm) was again applied, and the amplitude of the fEPSP after NEM treatment was compared with the fEPSP amplitude before NEM treatment. Baclofen (10 μm) was also applied following NEM treatment as a positive control and the extent of inhibition compared with that produced by baclofen in the absence of NEM in additional brain slices from the same animals. Incubation of slices with NEM was sufficient to inactivate Gi/o proteins, as effects of baclofen were significantly inhibited (Fig. 7B). Similar to its effects on baclofen inhibition, NEM also blocked muscarine inhibition (Fig. 7B). The similarity in the effect of NEM on baclofen and muscarine inhibition suggests that both agents act at PL input through Gi/o protein-dependent mechanisms and supports the conclusion that muscarine inhibits glutamate release at PL input through Gi/o-coupled presynaptic M4 receptors.
Muscarinic inhibition in the PL and MTN pathways occurs through mechanisms independent of GABAB receptors
An alternative explanation for the above findings is that muscarine acts on M3 receptors on GABAergic interneurons to increase interneuron excitability, releasing GABA, which acts on GABAB receptors to suppress synaptic transmission. This mechanism has recently been reported in hippocampal area CA1 (Goswamee and McQuiston, 2019). NEM would suppress this effect by blocking the action of Gi/o protein-coupled GABAB receptors. However, as our experiments are performed in the presence of picrotoxin and CGP55845, GABAA and GABAB receptors were not required for muscarinic inhibition at PL or MTN inputs to BLa. To determine whether muscarinic inhibition was greater when GABAB receptors were available, we compared the extent of inhibition by muscarine in the absence and presence of CGP55845. Bath application of CGP55845 (2 μm) had no effect on muscarine inhibition in either pathway, indicating that, although presynaptic GABAB receptors are present, muscarine suppression of glutamatergic fEPSPs at PL and MTN inputs is independent of GABAB receptors (Fig. 7C,D). Similarly, in whole-cell experiments, blockade of GABAergic inhibition by addition of picrotoxin (50 μm) and CGP55845 (5 μm) did not alter either the early facilitation (ACSF, 111.3 + 2.8% vs GABA blockers, 110.7 + 2.0%, n = 3, p = 0.87, paired t test) or late inhibition (ACSF, 84.5 + 3.3% vs GABA blockers, 82.2 + 3.7%, n = 5, p = 0.2, paired t test) produced by stimulation of cholinergic terminals, indicating that ACh did not act through GABAergic mechanisms to produce its effects.
Muscarine inhibits MTN inputs through an M3 receptor-dependent facilitation of retrograde endocannabinoid (eCB) signaling
eCBs serve a retrograde inhibitory role in many brain regions (Ohno-Shosaku and Kano, 2014), allowing neurons to regulate their upstream neuronal inputs. Postsynaptic Gq-coupled muscarinic (M1/M3) receptors can facilitate retrograde eCB release, suppressing GABA (Kim et al., 2002; Ohno-Shosaku et al., 2003) or glutamate transmission (Chiu and Castillo, 2008; Kodirov et al., 2009). While this mechanism has not previously been reported at excitatory terminals in the BLa, it is possible that postsynaptic M3 receptors on BLa pyramidal cells could act through retrograde eCB release to inhibit glutamatergic transmission in the MTN or PL pathway. To examine this possibility, the selective CB1 antagonist, AM251 (1 μm), was applied in the presence of muscarine. At PL input, antagonism of CB1 receptors had no effect on muscarine inhibition (Fig. 8A). This lack of effect was somewhat surprising given the presence of CB1 receptors at these inputs, as application of CB1 receptor agonist WIN55212 (5 μm) suppressed PL-evoked fEPSPs in a manner reversible by AM251 (Fig. 8B). These data suggest that, although CB1 receptors can inhibit PL evoked fEPSPs in the BLa, muscarinic suppression of PL input is not CB1 receptor-dependent. Given the presence of CB2 receptors in the brain (Onaivi et al., 2008) and the ability of CB2 receptors to suppress transmitter release in some brain regions (Foster et al., 2016), in separate experiments, we also tested the effect of the CB2 antagonist, AM630. However, as with CB1 antagonists, AM630 (2 μm) had no effect on muscarine inhibition (Musc: 30.1 + 5.2%; Musc + AM630: 27.7 + 2.0%; n = 3; N = 3; p = 0.75, paired t test). In contrast, at MTN inputs blockade of CB1 receptors with AM251 completely reversed muscarinic inhibition of fEPSPs (Fig. 8C), while having no effect on baseline fEPSPs in the absence of muscarine (Fig. 8D). Muscarine inhibition at MTN input is dependent on M3 receptors (Fig. 6). These findings suggest that at MTN inputs, muscarine inhibition is mediated by a postsynaptic M3 receptor-mediated release of eCBs, which retrogradely acts on CB1 receptors on MTN terminals to inhibit glutamatergic transmission (Fig. 8E).
Frequency-dependent inhibition of glutamatergic input by mAChRs
PL and MTN inputs are differentially modulated by mAChRs in response to single pulse stimulation. However, theta (4-12 Hz) and γ (30-80 Hz) frequency activity occurs in the BLa during emotional behavior and associative learning (Stujenske et al., 2014; Bocchio et al., 2017), making it of considerable interest to understand how ACh regulates afferent synaptic transmission at different frequencies in each pathway. Therefore, we investigated the effect of muscarine on responses in PL and MTN pathways to short stimulus trains at frequencies within a behaviorally relevant range in vivo. Stimulus trains consisting of 10 light pulses were delivered to either input at frequencies ranging from 1 to 40 Hz in the absence or presence of muscarine (10 μm). At PL synapses, stimulation at 1 Hz evoked responses of similar amplitude throughout the train. Muscarine (10 μm) strongly and similarly suppressed each response of the train (Fig. 9A,B). Alternately, stimulation at 40 Hz evoked a facilitation on the second response of the train (Fig. 9A,B) in line with earlier results showing paired pulse facilitation in this pathway (Figs. 3 and 5). Subsequent pulses in the train evoked progressively smaller fEPSPs such that the last fEPSP was 35.5 ± 2.8% of the amplitude of the first fEPSP. Following addition of muscarine, the first response of the train was strongly inhibited, as seen with single pulses, but subsequent responses were facilitated relative to the first fEPSP. This facilitation was maintained throughout the remainder of the train, such that the fEPSP amplitude in response to the last pulse of the train in muscarine was similar to the fEPSP amplitude to the last pulse in control (Fig. 9A,B), reflecting a complete loss of muscarine inhibition during the train. When comparing the extent of muscarine inhibition on the last pulse of different frequency trains, it could be seen that muscarine inhibition during the train was frequency-dependent (Fig. 9C). Inhibition was preserved during low-frequency 1 Hz stimulation, but increasingly attenuated as the frequency of the train increased. At 40 Hz, a frequency in the γ range, inhibition was completely lost during the train. A similar result was also found at MTN input. As seen with single pulses, muscarine inhibition was significantly less in this pathway compared with PL input. However, as in the PL pathway, this muscarine inhibition was preserved during low-frequency (1–5 Hz) trains, but attenuated during trains with frequencies >5 Hz, reaching a complete loss of inhibition at 40 Hz. Thus, at both PL and MTN inputs, muscarinic receptors act as a high pass filter, blocking low-frequency signals, while allowing higher-frequency signals to reach the BLa.
Discussion
Our results show robust ACh regulation of afferent input to BLa that is pathway-specific and frequency-dependent. ACh released by single pulse stimulation of cholinergic terminals engaged both nAChRs and mAChRs, producing a biphasic excitatory-inhibitory modulation of cortical input in the BLa. By contrast, elevation of extracellular ACh by blockade of acetylcholinesterase produced solely monophasic muscarinic inhibition of cortical input. At thalamic input in the same brain slices, this increase in extracellular ACh had no net effect on synaptic transmission. The differences in sensitivity of cortical and thalamic inputs to muscarinic inhibition were attributed to distinct mechanisms of mAChR action at each site. Muscarine inhibition at both inputs disappeared at higher frequencies of stimulation, consistent with its action as a high pass filter for afferent BLa signals.
Pharmacological studies with persistent agonist application have demonstrated nicotinic and muscarinic receptor regulation of transmitter release in the BLa (Sugita et al., 1991; Yajeya et al., 2000; Jiang and Role, 2008). The present study extends those findings by showing rapid regulation of glutamatergic transmission by endogenously released ACh. These findings are consistent with anatomic studies showing cholinergic terminals converging on glutamatergic synapses in BLa (Li et al., 2001; Muller et al., 2011). Single pulse stimulation of cholinergic terminals produced an immediate (<20 ms) and short-lived nAChR-mediated facilitation of cortical input to BLa, followed by a slower mAChR-mediated inhibition, lasting for up to 1 s. Both facilitation and inhibition of afferent input were evoked by the same single cholinergic stimulus. Prior studies have reported postsynaptic responses to individual cholinergic stimuli in inhibitory neurons in thalamus and cortex (Sun et al., 2013; Urban-Ciecko et al., 2018). However, to our knowledge, this is the first study that demonstrates that ACh release can potentiate glutamate release on the timescale of an individual synaptic event. This is also the first demonstration of this form of excitatory-inhibitory neuromodulation by ACh in the amygdala and suggests that cholinergic neuromodulation can serve precise, computational roles in the BLa network. The presence of these forms of cholinergic modulation in BLa is consistent with the robust cholinergic innervation of this region and further supports the vital role of ACh in information processing in this region.
Cholinergic neurons in BF exhibit fast and precise responses to both appetitive and aversive behavioral cues (Hangya et al., 2015). Studies using fluorescent ACh sensors have found that, during emotionally salient stimuli, phasic release of ACh into the BLa (Crouse et al., 2020) mediates associative learning (Jiang et al., 2016). In addition, phasic BF cholinergic stimulation can induce acute appetitive behaviors (Aitta-aho et al., 2018). It is tempting to speculate that the excitatory-inhibitory modulation of glutamatergic transmission by endogenously released ACh observed here underlies the action of phasically released ACh in the BLa during these behaviors. Phasic ACh rapidly engaged nAChRs on cortical terminals in BLa to facilitate glutamate release for up to 20 ms following cholinergic terminal activation. In contrast, glutamate release 50-1000 ms after simulation of cholinergic inputs was suppressed by robust mAChR-mediated inhibition. Together, the biphasic action of endogenously released ACh on presynaptic nicotinic and muscarinic receptors suggests that it would entrain glutamatergic input in a tight temporal window following cholinergic terminal activation and suppress poorly timed input that arrived outside of this window. This mechanism would enhance the signal-to-noise ratio for cortical input to BLa, thereby facilitating attention to salient signals (Bloem et al., 2014; Dannenberg et al., 2017) and may be important in forms of heterosynaptic plasticity in the BLa (Jiang et al., 2016).
ACh release from the BF occurs at multiple physiological timescales, ranging from milliseconds to hours (Disney and Higley, 2020; Sarter and Lustig, 2020). To better understand the consequences of sustained ACh elevation on glutamate transmission, we increased extracellular ACh by inhibiting acetylcholinesterase with physostigmine. In contrast to phasic ACh, sustained ACh elevation produced a steady state and reversible monophasic inhibition of cortical input. This inhibition was concentration-dependent, such that ACh elevation produced by even a low concentration of physostigmine inhibited cortical input. The inhibition was also mAChR-mediated as it was entirely reversed by atropine and unaffected by nAChR blockade. The lack of nAChR involvement is likely attributed to nAChR desensitization during sustained ACh, which has been well documented for these receptors (Quick and Lester, 2002; Giniatullin et al., 2005). In contrast to its effects at cortical inputs, under the same conditions and in the same brain slices, sustained ACh elevation produced little net effect at MTN input. The lack of effect was associated with both a larger persistent nicotinic facilitation and a smaller muscarinic inhibition that opposed and occluded each other. The persistence of nAChR-mediated facilitation at MTN input during elevated ACh may reflect distinct nAChR types at MTN compared with PL synapses (Quick and Lester, 2002; Venkatesan and Lambe, 2020) or differences in the anatomic arrangement of cholinergic release sites and thalamic terminals (Disney and Higley, 2020). This could result in lower concentrations of ACh at MTN synapses which would be less likely to desensitize nAChRs.
In addition to differences in nicotinic facilitation, MTN synapses were also subject to significantly less muscarinic inhibition than PL input. This disparity was caused by differential regulation of transmitter release by M4 and M3 receptors at the two inputs. These findings are consistent with growing evidence of highly specific localization of muscarinic receptor types to distinct neural pathways in the brain (Gil et al., 1997; Palacios-Filardo et al., 2021). The finding that M4 receptors regulate PL input is the first demonstration of presynaptic inhibition by M4 receptors in the BLa and builds on prior work showing presynaptic regulation by M4 receptors in other brain regions (Dasari and Gulledge, 2011; Pancani et al., 2014; Yang et al., 2020; Palacios-Filardo et al., 2021). Inhibition by M4 receptors was likely mediated by a suppression of presynaptic N- and P-type voltage-gated calcium channels through a Gi/o protein-dependent mechanism (Hille, 1994; Howe and Surmeier, 1995; Yan and Surmeier, 1996). Blockade of muscarinic inhibition by NEM in the present study supports this conclusion (see also Shapiro et al., 1994). Muscarinic modulation of these calcium channels is voltage-dependent and is attenuated by membrane depolarization (Yan and Surmeier, 1996). This voltage dependence could underlie the observed loss of muscarinic inhibition during high-frequency stimulation when the presynaptic membrane would be depolarized. This mechanism could explain why low-frequency transmission at cortical inputs would be suppressed by presynaptic mAChRs, but high-frequency or burst transmission would pass. Presynaptic mAChRs would thereby serve as a high pass filter for incoming salient information from cortex.
In contrast, at MTN inputs muscarinic inhibition is mediated by M3 receptors. The differences in muscarinic receptor type at PL and MTN inputs provide a mechanism for differential sensitivity to ACh in these two pathways and are consistent with the finding that muscarine was significantly less potent at MTN than PL input. Our data indicate that ACh suppressed glutamate release at MTN inputs by acting on postsynaptic M3 receptors to stimulate retrograde eCB release which subsequently engaged CB1 receptors on thalamic terminals. This conclusion is supported by the ability of an M3 receptor antagonist to block the inhibition and the inability of muscarine to produce inhibition in the presence of a CB1 receptor antagonist. Muscarinic receptor-induced suppression of excitation has been reported (Chiu and Castillo, 2008; Kodirov et al., 2009) but has not been demonstrated in BLa. However, its role in this region is consistent with both the high expression of CB1 receptors in the amygdala (Marsicano and Lutz, 1999) and the presence of these receptors in glutamatergic terminals in this area (Domenici et al., 2006; Fitzgerald et al., 2019). Our data show that a CB1 receptor agonist suppressed transmission at both PL and MTN inputs, indicating the presence of CB1 receptors at both glutamatergic synapses. The presence of muscarinic receptor-induced suppression of excitation only at MTN input thus reflects the localization of M3 receptors capable of stimulating eCB release. These findings highlight the pathway-specific control of glutamate release by distinct cholinergic receptors and provide targets to selectively modulate individual components of ACh's actions.
The marked difference in muscarinic inhibition at PL and MTN inputs suggests that during behavioral states associated with high cholinergic tone, thalamic input will more strongly influence BLa activity than will cortical input. These findings are consistent with prior work in cortex showing that ACh enhances the influence of thalamic sensory input on cortical activity through a nicotinic facilitation of glutamate release, and reduces internal corticocortical connections by presynaptic muscarinic inhibition (Hasselmo, 2006; Hasselmo and Sarter, 2011). The resulting reduction in cortical feedback excitation is postulated to reduce interference from previous retrieval and thereby enhance memory encoding and attention to novel sensory input. The differential cholinergic modulation of PL and MTN inputs seen in the present study may similarly favor thalamic sensory input and reduce cortical feedback in amygdala during behavioral states associated with high cholinergic tone. In this way, ACh would prioritize amygdala inputs to facilitate encoding of emotional memories and attention to novel cues.
Footnotes
This work was supported by National Institute of Mental Health R01MH104638 to D.D.M. and A.J.M.; University of South Carolina VP for Research ASPIRE2 Award to D.D.M.; University of South Carolina VP for Research Support to Promote the Advancement of Research and Creativity (SPARC) Research Grant; National Institutes of Health-NIGMS Grant T32-GM081740 to S.C.T.; and supported in part by Merit Award I01 BX001374 to Marlene A. Wilson (Principal Investigator) from the U.S. Department of Veterans Affairs Biomedical Laboratory Research and Development Service.
The authors declare no competing financial interests.
- Correspondence should be addressed to David D. Mott at david.mott{at}uscmed.sc.edu