This confocal image from a postnatal day 26 mouse brain shows three cortical astrocytes labeled with GFAP (red) and membrane-targeted GFP (AAV5-gfaABC1D-lckGFP; cyan). Rosenberg et al. used this technique to analyze the morphology of individual astrocytes as they investigated the role of noradrenergic signaling in astrocyte maturation. See the manuscript by Rosenberg et al. (pages 8621–8636) for more information. Cover image: Marci Rosenberg.