Figure 4. Newly deorphanized LGICs are expressed broadly in the nervous system. A–E, Fluorescent reporters of intercistonically spliced mKate2 or GFP driven under the promoter and/or genomic sequence reveals broad neuronal expression of cholinergic channels with little overlap. lgc-40 expression was identified in OLQs, BAG, M2, MC, ALM, PVM, and PLM. lgc-57 expression was identified in M3, RIP, URY, AIB, RMH, SMB, ADA, ventral cord neurons, and PVR. lgc-58 expression was identified in I3, IL1, CEPs, URA, URB, SIA, ADE, RIS, RMG, VC3-6, PVQ, and PHC. lgc-49 expression was identified in CEPs, URX, AFD, ASJ, RIG, PVQ, PVC, PLN, and ALN. lgc-39 expression was identified in URB, RIA, AVA, AVD, M1, AQR, AVK, AS, VA, and DA. F, Schematic depicting a subset of the synaptic connections received by the lgc-39-expressing AVA neurons; numbers in brackets show the total number of synapses for each connection (Extended Data Fig. 4-1, cell ID of still orphan LGCs; Extended Data Tables 4-1, strain list, 4-2, primer list, 4-3, expression overlap compared with the CeNGEN dataset).