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Research Articles, Neurobiology of Disease

Ciliary ARL13B Is Essential for Vision and Morphogenesis of Cone Outer Segments

Alexis Crockett, Sierra Kuzak, Thamaraiselvi Saravanan, Tongju Guan, Tamara Caspary, Hunter Aliff, George Holmes, Faezeh Moakedi and Visvanathan Ramamurthy
Journal of Neuroscience 20 August 2025, 45 (34) e0752252025; https://doi.org/10.1523/JNEUROSCI.0752-25.2025
Alexis Crockett
1Departments of Neuroscience, West Virginia University, School of Medicine, Morgantown, West Virginia 26506
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Sierra Kuzak
2Biochemistry and Molecular Medicine, West Virginia University, School of Medicine, Morgantown, West Virginia 26506
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Thamaraiselvi Saravanan
2Biochemistry and Molecular Medicine, West Virginia University, School of Medicine, Morgantown, West Virginia 26506
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Tongju Guan
2Biochemistry and Molecular Medicine, West Virginia University, School of Medicine, Morgantown, West Virginia 26506
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Tamara Caspary
3Department of Human Genetics, Emory University School of Medicine, Atlanta, Georgia 30322
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Hunter Aliff
2Biochemistry and Molecular Medicine, West Virginia University, School of Medicine, Morgantown, West Virginia 26506
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George Holmes
2Biochemistry and Molecular Medicine, West Virginia University, School of Medicine, Morgantown, West Virginia 26506
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Faezeh Moakedi
2Biochemistry and Molecular Medicine, West Virginia University, School of Medicine, Morgantown, West Virginia 26506
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Visvanathan Ramamurthy
2Biochemistry and Molecular Medicine, West Virginia University, School of Medicine, Morgantown, West Virginia 26506
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Article Information

DOI 
https://doi.org/10.1523/JNEUROSCI.0752-25.2025
PubMed 
40721319
Published By 
Society for Neuroscience
History 
  • Received April 14, 2025
  • Revision received June 26, 2025
  • Accepted July 14, 2025
  • First published July 28, 2025.
  • Version of record published August 20, 2025.
Copyright & Usage 
Copyright © 2025 the authors SfN exclusive license.

Author Information

  1. Alexis Crockett1,
  2. Sierra Kuzak2,
  3. Thamaraiselvi Saravanan2,
  4. Tongju Guan2,
  5. Tamara Caspary3,
  6. Hunter Aliff2,
  7. George Holmes2,
  8. Faezeh Moakedi2, and
  9. Visvanathan Ramamurthy2
  1. 1Departments of Neuroscience, West Virginia University, School of Medicine, Morgantown, West Virginia 26506
  2. 2Biochemistry and Molecular Medicine, West Virginia University, School of Medicine, Morgantown, West Virginia 26506
  3. 3Department of Human Genetics, Emory University School of Medicine, Atlanta, Georgia 30322
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Author contributions

  1. Author contributions: A.C. and V.R. designed research; A.C., S.K., T.S., T.G., H.A., G.H., and F.M. performed research; T.C. contributed unpublished reagents/analytic tools; A.C., S.K., and V.R. analyzed data; A.C. wrote the paper.

Disclosures

    • Received April 14, 2025.
    • Revision received June 26, 2025.
    • Accepted July 14, 2025.
  • This work was supported by National Institutes of Health grants R01EY028035 (V.R.), R01EY031346 (V.R.), and R35 GM148416 (T.C.), a challenge grant from Research to Prevent Blindness (to WVU); Visual Sciences Center of Biomedical Research Excellence (VS-COBRE grant P20GM144230); and US National Science Foundation (award #2125872) NRT-HDR: Bridges in Digital Health. Antibodies for TULP1 were gifted by Dr Stephanie Hagstrom and antibodies for IFT88 and IFT40 were gifted by Dr. Gregory Pazour.

  • The authors declare no competing financial interests.

  • This paper contains supplemental material available at: https://doi.org/10.1523/JNEUROSCI.0752-25.2025

  • Correspondence should be addressed to Visvanathan Ramamurthy at ramamurthyv{at}hsc.wvu.edu.

Funding

  • HHS | NIH | National Eye Institute (NEI)

    R01EY028035; R01EY031346
  • HHS | NIH | National Institute of General Medical Sciences (NIGMS)

    P20GM144230
  • Research to Prevent Blindness (RPB)

  • National Science Foundation (NSF)

Other Version

  • previous version (July 28, 2025).
  • You are viewing the most recent version of this article.

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The Journal of Neuroscience: 45 (34)
Journal of Neuroscience
Vol. 45, Issue 34
20 Aug 2025
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Ciliary ARL13B Is Essential for Vision and Morphogenesis of Cone Outer Segments
Alexis Crockett, Sierra Kuzak, Thamaraiselvi Saravanan, Tongju Guan, Tamara Caspary, Hunter Aliff, George Holmes, Faezeh Moakedi, Visvanathan Ramamurthy
Journal of Neuroscience 20 August 2025, 45 (34) e0752252025; DOI: 10.1523/JNEUROSCI.0752-25.2025

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Ciliary ARL13B Is Essential for Vision and Morphogenesis of Cone Outer Segments
Alexis Crockett, Sierra Kuzak, Thamaraiselvi Saravanan, Tongju Guan, Tamara Caspary, Hunter Aliff, George Holmes, Faezeh Moakedi, Visvanathan Ramamurthy
Journal of Neuroscience 20 August 2025, 45 (34) e0752252025; DOI: 10.1523/JNEUROSCI.0752-25.2025
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Keywords

  • axoneme
  • blindness
  • cilia
  • cone photoreceptor
  • Joubert syndrome
  • photoreceptor

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