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Research Articles, Cellular/Molecular

Active Zone Maturation Controls Presynaptic Output and Release Mode and Is Regulated by Neuronal Activity

Yulia Akbergenova, Jessica Matthias, Sofya Makeyeva and J. Troy Littleton
Journal of Neuroscience 26 November 2025, 45 (48) e1143252025; https://doi.org/10.1523/JNEUROSCI.1143-25.2025
Yulia Akbergenova
1Departments of Brain and Cognitive Sciences, The Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
2Biology, The Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
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Jessica Matthias
3Abberior Instruments America, Bethesda, Maryland 20814
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Sofya Makeyeva
1Departments of Brain and Cognitive Sciences, The Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
2Biology, The Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
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J. Troy Littleton
1Departments of Brain and Cognitive Sciences, The Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
2Biology, The Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
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Article Information

DOI 
https://doi.org/10.1523/JNEUROSCI.1143-25.2025
PubMed 
41087171
Published By 
Society for Neuroscience
History 
  • Received June 10, 2025
  • Revision received August 25, 2025
  • Accepted September 16, 2025
  • First published October 14, 2025.
  • Version of record published November 26, 2025.
Copyright & Usage 
Copyright © 2025 Akbergenova et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Yulia Akbergenova1,2,
  2. Jessica Matthias3,
  3. Sofya Makeyeva1,2, and
  4. J. Troy Littleton1,2
  1. 1Departments of Brain and Cognitive Sciences, The Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
  2. 2Biology, The Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
  3. 3Abberior Instruments America, Bethesda, Maryland 20814
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Author contributions

  1. Author contributions: Y.A. and J.T.L. designed research; Y.A., J.M., and S.M. performed research; Y.A. and J.T.L. analyzed data; Y.A. and J.T.L. wrote the paper.

Disclosures

    • Received June 10, 2025.
    • Revision received August 25, 2025.
    • Accepted September 16, 2025.
  • We thank the Bloomington Drosophila Stock Center (Indiana University; NIH P40OD018537), the Developmental Studies Hybridoma Bank (University of Iowa), Gerry Rubin, Kate O’Connor-Giles, Ethan Graf, Stephan Sigrist, and Dion Dickman for providing Drosophila stocks or antisera and Dina Volfson for the assistance with strain generation. This work was supported by the Freedom Together Foundation and NIH Grants MH104536 and NS117588 to J.T.L.

  • J.M. is an employee of Abberior Instruments America, which commercializes super-resolution microscopy systems, including MINFLUX.

  • This paper contains supplemental material available at: https://doi.org/10.1523/JNEUROSCI.1143-25.2025

  • Correspondence should be addressed to Yulia Akbergenova at yulakb{at}mit.edu or J. Troy Littleton at troy{at}mit.edu.

Funding

  • HHS | NIH | National Institute of Mental Health (NIMH)

    MH104536
  • HHS | NIH | National Institute of Neurological Disorders and Stroke (NINDS)

    NS117588
  • Freedom Together Foundation

Other Version

  • previous version (October 14, 2025).
  • You are viewing the most recent version of this article.

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The Journal of Neuroscience: 45 (48)
Journal of Neuroscience
Vol. 45, Issue 48
26 Nov 2025
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Active Zone Maturation Controls Presynaptic Output and Release Mode and Is Regulated by Neuronal Activity
Yulia Akbergenova, Jessica Matthias, Sofya Makeyeva, J. Troy Littleton
Journal of Neuroscience 26 November 2025, 45 (48) e1143252025; DOI: 10.1523/JNEUROSCI.1143-25.2025

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Active Zone Maturation Controls Presynaptic Output and Release Mode and Is Regulated by Neuronal Activity
Yulia Akbergenova, Jessica Matthias, Sofya Makeyeva, J. Troy Littleton
Journal of Neuroscience 26 November 2025, 45 (48) e1143252025; DOI: 10.1523/JNEUROSCI.1143-25.2025
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Keywords

  • active zone
  • Drosophila
  • neurotransmitter release
  • NMJ
  • synapse
  • synaptic vesicle fusion

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