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Isolated frog olfactory cilia: a preparation of dendritic membranes from chemosensory neurons

Z Chen, U Pace, J Heldman, A Shapira and D Lancet
Journal of Neuroscience 1 August 1986, 6 (8) 2146-2154; https://doi.org/10.1523/JNEUROSCI.06-08-02146.1986
Z Chen
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U Pace
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J Heldman
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A Shapira
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D Lancet
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Abstract

The recently introduced frog olfactory cilia preparation (Chen and Lancet, 1984; Pace et al., 1985) has been useful for studies of molecular chemosensory mechanisms. Here we describe in detail the properties of this cilia preparation. The “calcium shock” procedure leads to a complete removal of the cilia from the olfactory epithelial surface. Isolated cilia constitute segments of proximal regions with 9 X 2 + 2 microtubular arrangement and a large proportion of membrane vesicles, probably derived from the ciliary distal segments. Polypeptides unique to the olfactory cilia preparation, compared to a control preparation of palate respiratory cilia, are identified by Coomassie brilliant blue staining, silver staining, and radiolabeled lectin overlays, as well as by biosynthetic labeling with 35S- methionine in epithelial explants and protein phosphorylation in isolated cilia. The olfactory cilia preparation contains odorant- sensitive adenylate cyclase, which is absent in control membranes from deciliated epithelium. High activities of tyrosine and serine/threonine protein kinases are also present. The olfactory cilia preparation described should be instrumental in the further elucidation of the biochemistry and molecular biology of vertebrate olfaction.

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The Journal of Neuroscience: 6 (8)
Journal of Neuroscience
Vol. 6, Issue 8
1 Aug 1986
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Isolated frog olfactory cilia: a preparation of dendritic membranes from chemosensory neurons
Z Chen, U Pace, J Heldman, A Shapira, D Lancet
Journal of Neuroscience 1 August 1986, 6 (8) 2146-2154; DOI: 10.1523/JNEUROSCI.06-08-02146.1986

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Isolated frog olfactory cilia: a preparation of dendritic membranes from chemosensory neurons
Z Chen, U Pace, J Heldman, A Shapira, D Lancet
Journal of Neuroscience 1 August 1986, 6 (8) 2146-2154; DOI: 10.1523/JNEUROSCI.06-08-02146.1986
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