Skip to main content

Main menu

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Collections
    • Podcast
  • ALERTS
  • FOR AUTHORS
    • Information for Authors
    • Fees
    • Journal Clubs
    • eLetters
    • Submit
    • Special Collections
  • EDITORIAL BOARD
    • Editorial Board
    • ECR Advisory Board
    • Journal Staff
  • ABOUT
    • Overview
    • Advertise
    • For the Media
    • Rights and Permissions
    • Privacy Policy
    • Feedback
    • Accessibility
  • SUBSCRIBE

User menu

  • Log out
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Neuroscience
  • Log out
  • Log in
  • My Cart
Journal of Neuroscience

Advanced Search

Submit a Manuscript
  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Collections
    • Podcast
  • ALERTS
  • FOR AUTHORS
    • Information for Authors
    • Fees
    • Journal Clubs
    • eLetters
    • Submit
    • Special Collections
  • EDITORIAL BOARD
    • Editorial Board
    • ECR Advisory Board
    • Journal Staff
  • ABOUT
    • Overview
    • Advertise
    • For the Media
    • Rights and Permissions
    • Privacy Policy
    • Feedback
    • Accessibility
  • SUBSCRIBE
PreviousNext
Articles

Visualization of endogenous glycine in cat retina: an immunocytochemical study with Fab fragments

RG Pourcho and DJ Goebel
Journal of Neuroscience 1 April 1987, 7 (4) 1189-1197; https://doi.org/10.1523/JNEUROSCI.07-04-01189.1987
RG Pourcho
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DJ Goebel
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Fab fragments of a glycine antiserum were prepared and used for immunocytochemical visualization of glycine in the cat retina. The use of Fab fragments in conjunction with Fab-specific secondary and tertiary antisera improved tissue penetration and made it possible to identify a number of the immunoreactive neurons. Staining was observed in several subpopulations of amacrine cells, including the A7(AII) rod amacrine. Multiple subpopulations of cone bipolar cells were also seen to be immunoreactive. Many neurons exhibited no detectable immunostaining, indicating that general metabolic levels of glycine do not interfere with the visualization of those cells that contain large amounts of endogenous glycine. The distribution of immunostaining appears to parallel the pattern of glycine labeling seen previously with autoradiographic techniques and implicates these cells in glycine- mediated neurotransmission.

Back to top

In this issue

The Journal of Neuroscience: 7 (4)
Journal of Neuroscience
Vol. 7, Issue 4
1 Apr 1987
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
Email

Thank you for sharing this Journal of Neuroscience article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Visualization of endogenous glycine in cat retina: an immunocytochemical study with Fab fragments
(Your Name) has forwarded a page to you from Journal of Neuroscience
(Your Name) thought you would be interested in this article in Journal of Neuroscience.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
View Full Page PDF
Citation Tools
Visualization of endogenous glycine in cat retina: an immunocytochemical study with Fab fragments
RG Pourcho, DJ Goebel
Journal of Neuroscience 1 April 1987, 7 (4) 1189-1197; DOI: 10.1523/JNEUROSCI.07-04-01189.1987

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Respond to this article
Request Permissions
Share
Visualization of endogenous glycine in cat retina: an immunocytochemical study with Fab fragments
RG Pourcho, DJ Goebel
Journal of Neuroscience 1 April 1987, 7 (4) 1189-1197; DOI: 10.1523/JNEUROSCI.07-04-01189.1987
Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Responses to this article

Respond to this article

Jump to comment:

No eLetters have been published for this article.

Related Articles

Cited By...

More in this TOC Section

  • Memory Retrieval Has a Dynamic Influence on the Maintenance Mechanisms That Are Sensitive to ζ-Inhibitory Peptide (ZIP)
  • Neurophysiological Evidence for a Cortical Contribution to the Wakefulness-Related Drive to Breathe Explaining Hypocapnia-Resistant Ventilation in Humans
  • Monomeric Alpha-Synuclein Exerts a Physiological Role on Brain ATP Synthase
Show more Articles
  • Home
  • Alerts
  • Follow SFN on BlueSky
  • Visit Society for Neuroscience on Facebook
  • Follow Society for Neuroscience on Twitter
  • Follow Society for Neuroscience on LinkedIn
  • Visit Society for Neuroscience on Youtube
  • Follow our RSS feeds

Content

  • Early Release
  • Current Issue
  • Issue Archive
  • Collections

Information

  • For Authors
  • For Advertisers
  • For the Media
  • For Subscribers

About

  • About the Journal
  • Editorial Board
  • Privacy Notice
  • Contact
  • Accessibility
(JNeurosci logo)
(SfN logo)

Copyright © 2025 by the Society for Neuroscience.
JNeurosci Online ISSN: 1529-2401

The ideas and opinions expressed in JNeurosci do not necessarily reflect those of SfN or the JNeurosci Editorial Board. Publication of an advertisement or other product mention in JNeurosci should not be construed as an endorsement of the manufacturer’s claims. SfN does not assume any responsibility for any injury and/or damage to persons or property arising from or related to any use of any material contained in JNeurosci.