RT Journal Article
SR Electronic
T1 Glycine-insensitive NMDA-sensitive receptor expressed in Xenopus oocytes by guinea pig cerebellar mRNA
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 2148
OP 2155
DO 10.1523/JNEUROSCI.10-07-02148.1990
VO 10
IS 7
A1 M Sekiguchi
A1 K Okamoto
A1 Y Sakai
YR 1990
UL http://www.jneurosci.org/content/10/7/2148.abstract
AB The electrophysiological and pharmacological properties of N-methyl-D- aspartate (NMDA)-sensitive receptors expressed in Xenopus oocytes by injection of total poly(A)+RNAs (mRNAs) from the cerebellum and cerebrum of guinea pigs were compared. The inward current induced by NMDA under voltage-clamp in cerebellar mRNA-injected oocytes was depressed in a voltage-dependent fashion by Mg2+ to show a negative slope conductance and selectively antagonized by D-2-amino-5- phosphonovalerate (D-APV) and phencyclidine (PCP). Glycine (0.01–10 microM) did not potentiate NMDA-induced currents in cerebellar mRNA- injected oocytes, while it potentiated NMDA-induced currents in cerebral mRNA-injected oocytes in a dose-dependent fashion. 6-Cyano-7- nitroquinoxaline-2,3-dione and 7-chlorokynure-nate suppressed the NMDA response but significantly less potently in cerebellar mRNA-injected oocytes than in cerebral mRNA-injected oocytes. These results suggest that the NMDA-sensitive receptor expressed in Xenopus oocytes by guinea pig cerebellar mRNA resembles the cerebral NMDA receptor in its high sensitivities to Mg2+, PCP, and D-APV, but it is distinct from the cerebral NMDA receptor in responsiveness to glycine.